Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis by Lucano Billy M., Feria Manuel A., Zatan Adrian E., Toledo Odalis E., Aguilar Jorge L., Diringer Benoit M., Castañeda Arnaldo E.

“…The assays included morphological evaluation, catalase, oxidase, hemolysis, pathogen antagonism, antimicrobial susceptibility, protease, and amylase tests. Seven isolates exhibited antagonism against one of the three pathogens tested (Aeromonas hydrophila, Streptococcus agalactiae, and Lactococcus garvieae). …”
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A combined use of Panax ginseng extract and the probiotic, Lactobacillus plantarum in the diet of Asian seabass, Lates calcarifer: Effects on growth, immunity and survival after ba... by Xiaojie Hou, Jianfeng Zhang, Neda Behmagham, Hamed Ghafarifarsani

“…The immune components of serum [total immunoglobulin (Ig), lysozyme (lyz), alternative complement activity (ACH50), myeloperoxidase (MPO), nitroblue tetrazolium (NBT), total protein] and mucus [protease, Ig, alkaline phosphatase (ALP), Lyz] were mostly elevated in fish supplemented with LAC-P, GINS (1 %) and LAC-P + GINS (0.1–1 %) (P < 0.01). …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

“…Coagulation factor XIa is a new serine-protease family drug target for next-generation anticoagulants. …”
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The Role of Complement System in Septic Shock by Jean Charchaflieh, Jiandong Wei, Georges Labaze, Yunfang Joan Hou, Benjamin Babarsh, Helen Stutz, Haekyung Lee, Samrat Worah, Ming Zhang

“…Our preliminary study of MBL-associated serine protease-2 (MASP-2) in septic shock patients indicated that acute decrease of MASP-2 in the early phase of septic shock might correlate with in-hospital mortality. …”
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Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil by Wei Yin, Le Wang, Qian Han Shang, Yi Xin Li, Wei Sa, Quan Min Dong, Jian Liang

“…Results Morchella inoculation significantly increased the alkaline nitrogen (AN) and available phosphorus (AP) contents, protease activity (PA), and alkaline phosphatase activity (APA) of Poa pratensis rhizosphere soil. …”
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Modelling the immunopathogenesis of HIV-1 infection and the effect of multidrug therapy: The role of fusion inhibitors in HAART by Gesham Magombedze, Winston Garira, Eddie Mwenje

“…Then we extend the basic model to incorporate the effectof three major categories of anti-HIV-1 drugs: fusion/entry inhibitors (FIs),reverse transcriptase inhibitors (RTIs), and protease inhibitors (PIs). Modelanalysis establishes that the actual drug efficacy of FIs, γ and of PIs, κ isthe same as their effective efficacies while the effective drug efficacy for theRTIs, γ εis dependent on the rate of transcription of the HIV-1 RNA to DNA,and the lifespan of infected CD4+ T cells where virions have only entered thecytoplasm and that this effective efficacy is less than the actual efficacy, ε. …”
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Genome Sequencing of a Mung Bean Plant Growth Promoting Strain of P. aeruginosa with Biocontrol Ability by Devaraj Illakkiam, Manoharan Shankar, Paramasivan Ponraj, Jeyaprakash Rajendhran, Paramasamy Gunasekaran

“…In addition, niche-specific genes such as phosphate solubilising 3-phytase, adhesins, pathway-specific transcriptional regulators, a diguanylate cyclase involved in cellulose synthesis, a receptor for ferrienterochelin, a DEAD/DEAH-box helicase involved in stress tolerance, chemotaxis/motility determinants, an HtpX protease, and enzymes involved in the production of a chromanone derivative with potent antifungal activity were identified.…”
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Insights into sensitizing and eliciting capacity of gastric and gastrointestinal digestion products of shrimp (Penaeus vannamei) proteins in BALB/c mice by Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Wang Li, Na Sun

“…Meanwhile, spleen index, mouse mast cell protease-1 (mMCP-1) concentration and proportion of degranulated mast cells were significantly reduced in the GDS/GIDS groups (P < 0.05); simultaneously, allergic symptoms, vascular permeability and histopathological changes of tissues were alleviated. …”
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Engineering a tumor-selective prodrug T-cell engager bispecific antibody for safer immunotherapy by Amelia C. McCue, Stephen J. Demarest, Karen J. Froning, Michael J. Hickey, Stephen Antonysamy, Brian Kuhlman

“…This can be accomplished by masking the anti-CD3 arm of the TCE with an autoinhibitory motif that is released by tumor-enriched proteases. Here, we solve the crystal structure of the antigen-binding fragment of a novel anti-CD3 antibody, E10, in complex with its epitope from CD3 and use this information to engineer a masked form of the antibody that can activate by the tumor-enriched protease matrix metalloproteinase 2 (MMP-2). …”
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Anaerobic fermentation of soybean meal by Bacillus subtilis ED-3-7 and its effect on the intestinal microbial community of chicken by Wei Liu, Wei Wang, Jia Li, Hongya Li, Tongguo Gao, Baocheng Zhu

“…A strain named ED-3-7 with a high protease-producing ability was screened in a previous study. …”
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Exploring proteins within the coccolith matrix by Craig J. Dedman, Nishant Chauhan, Alba González-Lanchas, Chloë Baldreki, Adam A. Dowle, Tony R. Larson, Renee B. Y. Lee, Rosalind E. M. Rickaby

“…Notably, several protein features were consistently seen across the examined coccolithophore species, including the cell signalling 14-3-3 domain, chromosome segregation SMC ATPase domain, as well as proteins involved in protein processing and protease inhibition. The copper-binding cupredoxin domain was observed in both Gephyrocapsa species, as well as other marine calcifiers, suggestive of a requirement of Cu in biomineralisation. …”
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Severe Plasmodium falciparum malaria is associated with circulating ultra-large von Willebrand multimers and ADAMTS13 inhibition. by Deirdre Larkin, Bas de Laat, P Vince Jenkins, James Bunn, Alister G Craig, Virginie Terraube, Roger J S Preston, Cynthia Donkor, George E Grau, Jan A van Mourik, James S O'Donnell

“…Concomitant with the increase in VWF:Ag and VWF:CB was a significant persistent reduction in the activity of the VWF-specific cleaving protease ADAMTS13 (approximately 55% of normal; p<0.005). …”
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Murine model for chronic rhinosinusitis: an interventional study by Amr F. Hamour, John JW. Lee, Ewa Wasilewski, Eric Monteiro, John M. Lee, Allan Vescan, Lakshmi P. Kotra

“…Experimental animals (n = 20) were induced a CRS disease state upon receiving intraperitoneal sensitization with ovalbumin (OVA), followed by intranasal OVA with Aspergillus oryzae protease. Analysis of complete blood count with differential, peripheral blood smear, and histological markers from the nasal cavity mucosa were performed. …”
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Pasting Properties of Various Waxy Rice Flours: Effect of α-Amylase Activity, Protein, and Amylopectin by Yuehui Wang, Kuijie Sun, Kai Zheng, Wenchang Zhu, Wenping Ding, Qingyun Lyv, Lei Chen, Guozhen Wang, Kun Zhuang, Xi Chen

“…Rice flours were divided into four groups (Vietnam indica (VI), Jiangxi indica (JI), Anhui japonica (AJ), and Dongbei japonica (DJ) group) and treated with AgNO3 solution, DL-dithiothreitol (DTT), or protease (n = 3). Results suggested that both α-amylase activity and protein significantly decrease the pasting viscosity of waxy rice flours. …”
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Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts by Xinyang LU, Yishu SUN, Tao XU, Longbin FAN, Yaning CHANG, Yingjun ZHOU

“…The results demonstrated that the optimal enzymatic hydrolysis process parameters for rose bee pollen were a solid-to-liquid ratio of 1:30, a temperature of 55 °C, pH6, a composite enzyme system addition of 2.0% (cellulase comprising a ratio of 7:5 with composite protease), and a reaction time of 9 h, resulting in the highest wall-disruption rate of 73.13%. …”
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The highly pathogenic strain of porcine deltacoronavirus disrupts the intestinal barrier and causes diarrhea in newborn piglets by Xin Yao, Wei-Hong Lu, Wen-Ting Qiao, Yu-Qian Zhang, Bao-Ying Zhang, Hui-Xin Li, Jin-Long Li

“…Protein functional analysis showed that the structural changes of the three regions changed the epitope of S protein, the O-GalNAc glycosylation site and the 3C-like protease cleavage site. In addition, oral administration of 107 TCID50 CH/LNFX/2022 to newborn piglets successfully reproduced obvious clinical signs of piglets, such as diarrhea and dehydration. …”
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Cathepsin K Deficiency Impaired Ischemia-Induced Neovascularization in Aged Mice by Xueling Yue, Haiying Jiang, Ying Xu, Manli Xia, Xian-Wu Cheng

“…Aging is a major risk factor for cardiovascular disease. Cysteine protease cathepsin K (CatK) has been implicated in the process of angiogenesis, but the exact roles of individual CatK in vessel formation during aging are poorly understood. …”
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Process optimization, texture and microstructure of novel kelp tofu by Xianjiang Ye, Li Chen, Zhichen Su, Xiaojuan Lin, Jicheng Chen

“…The maximum IC50 value (4.14 mg/mL) of kelp enzymatic hydrolysate was observed at enzyme (at an alkaline protease:trypsin ratio of 2:1) concentration 1.5 %, temperature 55 °C and time 2 h. …”
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Enhancing maize seed resistance to chilling stress through seed germination and surface morphological changes using high voltage electrostatic field by Yao Lu, Yaoyao Li, Qian Peng, Xiangyun Sun, Qinglu Yang, Zhanhua Song, Fuyang Tian, Yinfa Yan, Mochen Liu

“…Optimal HVEF treatment resulted in cell wall expansion, enhanced fiber elasticity, reduced interstitial spaces, and swollen cells, indicating improved hydrophilicity and protease activity. This study offers valuable insights into the mechanisms by which HVEF improves seed performance under low-temperature stress.…”
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C-28 linker length modulates the activity of second-generation HIV-1 maturation inhibitors by K. C. Yuvraj, Aradhana Singh, Sayantani Datta, Ritika Das, Pranjal Raj Saxena, Subash Chapagain, T. J. Nitz, Carl Wild, Ritu Gaur

“…These MIs were also active against integrase inhibitor-resistant viruses and protease inhibitor-resistant viruses. Our data has provided vital information that in addition to the nature of the functional group at C28 position of the MI, the length of linker contributes significantly to its activity. …”
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