Insights into sensitizing and eliciting capacity of gastric and gastrointestinal digestion products of shrimp (Penaeus vannamei) proteins in BALB/c mice by Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Wang Li, Na Sun

“…Meanwhile, spleen index, mouse mast cell protease-1 (mMCP-1) concentration and proportion of degranulated mast cells were significantly reduced in the GDS/GIDS groups (P < 0.05); simultaneously, allergic symptoms, vascular permeability and histopathological changes of tissues were alleviated. …”
Get full text

Engineering a tumor-selective prodrug T-cell engager bispecific antibody for safer immunotherapy by Amelia C. McCue, Stephen J. Demarest, Karen J. Froning, Michael J. Hickey, Stephen Antonysamy, Brian Kuhlman

“…This can be accomplished by masking the anti-CD3 arm of the TCE with an autoinhibitory motif that is released by tumor-enriched proteases. Here, we solve the crystal structure of the antigen-binding fragment of a novel anti-CD3 antibody, E10, in complex with its epitope from CD3 and use this information to engineer a masked form of the antibody that can activate by the tumor-enriched protease matrix metalloproteinase 2 (MMP-2). …”
Get full text

Anaerobic fermentation of soybean meal by Bacillus subtilis ED-3-7 and its effect on the intestinal microbial community of chicken by Wei Liu, Wei Wang, Jia Li, Hongya Li, Tongguo Gao, Baocheng Zhu

“…A strain named ED-3-7 with a high protease-producing ability was screened in a previous study. …”
Get full text

Severe Plasmodium falciparum malaria is associated with circulating ultra-large von Willebrand multimers and ADAMTS13 inhibition. by Deirdre Larkin, Bas de Laat, P Vince Jenkins, James Bunn, Alister G Craig, Virginie Terraube, Roger J S Preston, Cynthia Donkor, George E Grau, Jan A van Mourik, James S O'Donnell

“…Concomitant with the increase in VWF:Ag and VWF:CB was a significant persistent reduction in the activity of the VWF-specific cleaving protease ADAMTS13 (approximately 55% of normal; p<0.005). …”
Get full text

Murine model for chronic rhinosinusitis: an interventional study by Amr F. Hamour, John JW. Lee, Ewa Wasilewski, Eric Monteiro, John M. Lee, Allan Vescan, Lakshmi P. Kotra

“…Experimental animals (n = 20) were induced a CRS disease state upon receiving intraperitoneal sensitization with ovalbumin (OVA), followed by intranasal OVA with Aspergillus oryzae protease. Analysis of complete blood count with differential, peripheral blood smear, and histological markers from the nasal cavity mucosa were performed. …”
Get full text

Pasting Properties of Various Waxy Rice Flours: Effect of α-Amylase Activity, Protein, and Amylopectin by Yuehui Wang, Kuijie Sun, Kai Zheng, Wenchang Zhu, Wenping Ding, Qingyun Lyv, Lei Chen, Guozhen Wang, Kun Zhuang, Xi Chen

“…Rice flours were divided into four groups (Vietnam indica (VI), Jiangxi indica (JI), Anhui japonica (AJ), and Dongbei japonica (DJ) group) and treated with AgNO3 solution, DL-dithiothreitol (DTT), or protease (n = 3). Results suggested that both α-amylase activity and protein significantly decrease the pasting viscosity of waxy rice flours. …”
Get full text

Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts by Xinyang LU, Yishu SUN, Tao XU, Longbin FAN, Yaning CHANG, Yingjun ZHOU

“…The results demonstrated that the optimal enzymatic hydrolysis process parameters for rose bee pollen were a solid-to-liquid ratio of 1:30, a temperature of 55 °C, pH6, a composite enzyme system addition of 2.0% (cellulase comprising a ratio of 7:5 with composite protease), and a reaction time of 9 h, resulting in the highest wall-disruption rate of 73.13%. …”
Get full text

Cathepsin K Deficiency Impaired Ischemia-Induced Neovascularization in Aged Mice by Xueling Yue, Haiying Jiang, Ying Xu, Manli Xia, Xian-Wu Cheng

“…Aging is a major risk factor for cardiovascular disease. Cysteine protease cathepsin K (CatK) has been implicated in the process of angiogenesis, but the exact roles of individual CatK in vessel formation during aging are poorly understood. …”
Get full text

Process optimization, texture and microstructure of novel kelp tofu by Xianjiang Ye, Li Chen, Zhichen Su, Xiaojuan Lin, Jicheng Chen

“…The maximum IC50 value (4.14 mg/mL) of kelp enzymatic hydrolysate was observed at enzyme (at an alkaline protease:trypsin ratio of 2:1) concentration 1.5 %, temperature 55 °C and time 2 h. …”
Get full text

Enhancing maize seed resistance to chilling stress through seed germination and surface morphological changes using high voltage electrostatic field by Yao Lu, Yaoyao Li, Qian Peng, Xiangyun Sun, Qinglu Yang, Zhanhua Song, Fuyang Tian, Yinfa Yan, Mochen Liu

“…Optimal HVEF treatment resulted in cell wall expansion, enhanced fiber elasticity, reduced interstitial spaces, and swollen cells, indicating improved hydrophilicity and protease activity. This study offers valuable insights into the mechanisms by which HVEF improves seed performance under low-temperature stress.…”
Get full text

C-28 linker length modulates the activity of second-generation HIV-1 maturation inhibitors by K. C. Yuvraj, Aradhana Singh, Sayantani Datta, Ritika Das, Pranjal Raj Saxena, Subash Chapagain, T. J. Nitz, Carl Wild, Ritu Gaur

“…These MIs were also active against integrase inhibitor-resistant viruses and protease inhibitor-resistant viruses. Our data has provided vital information that in addition to the nature of the functional group at C28 position of the MI, the length of linker contributes significantly to its activity. …”
Get full text

Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen by Ling Zhang, Xiaocui Yang, Kaijun Xiao, Yuyi Lu, Chunhai Li, Zijin Zhang

“…The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. …”
Get full text

Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis by Lamiaa A. Salama, Hazem Hamed Saleh, Shaymaa H. Abdel-Rhman, Rasha Barwa, Ramadan Hassan

“…Different virulence factors as protease, urease and hemolysin production are detected. …”
Get full text

A multi-tissue longitudinal proteomics study to evaluate the suitability of post-mortem samples for pathophysiological research by Christian M. Beusch, Ken Braesch-Andersen, Ulrika Felldin, Pierre Sabatier, Anna Widgren, Jonas Bergquist, Karl-Henrik Grinnemo, Sergey Rodin

“…Additionally, we have conducted a comprehensive analysis of semi-tryptic peptides, revealing distinct consensus motifs for different organs, indicating organ-specific post-mortem protease activity. In conclusion, our findings emphasize the critical importance of considering PMI effects when designing proteomics studies, as these effects may significantly overshadow the impacts of diseases. …”
Get full text

Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. …”
Get full text

Research Progress on Enzymatic Preparation and Functional Properties of Bioactive Peptides in Hairtail by Yuanyuan WU, Kewei ZHENG, Wenzhu LI, Cheng LI, Xiaojun YAN, Qingbao MA, Wei JIANG

“…The antioxidative activity of enzymatic hydrolysates is extensively studied, with alkaline protease and papain being the most commonly used enzymes. …”
Get full text

Potential of Microbial Diversity of Coastal Sand Dunes: Need for Exploration in Odisha Coast of India by Shubhransu Nayak, Satyaranjan Behera, Prasad Kumar Dash

“…Many neutrophilic and alkaliphilic eubacterial species, endophytic fungi from dunes have proved their ability for the production of extracellular enzymes like cellulase, pectinase, amylase, protease, tannase, chitinase, etc., which are of great importance to various industries. …”
Get full text

Decreased TNF Levels and Improved Retinal Ganglion Cell Survival in MMP-2 Null Mice Suggest a Role for MMP-2 as TNF Sheddase by Lies De Groef, Manuel Salinas-Navarro, Griet Van Imschoot, Claude Libert, Roosmarijn E. Vandenbroucke, Lieve Moons

“…Pathological conditions in the CNS are therefore often related to imbalanced MMP activities and disturbances of the complex MMP-dependent protease network. Likewise, in the retina, various studies in animal models and human patients suggested MMPs to be involved in glaucoma. …”
Get full text

Virtual Screening for Potential Inhibitors of NS3 Protein of Zika Virus by Maheswata Sahoo, Lingaraja Jena, Sangeeta Daf, Satish Kumar

“…In this study, we performed molecular docking analysis of these drugs against nonstructural 3 (NS3) protein of ZIKV. The protease activity of NS3 is necessary for viral replication and its prohibition could be considered as a strategy for treatment of ZIKV infection. …”
Get full text

TMPRSS2 as a Key Player in Viral Pathogenesis: Influenza and Coronaviruses by Gilmara Barros de Lima, Everton Nencioni, Fábio Thimoteo, Camila Perea, Rafaela Fuzaro Alves Pinto, Sergio Daishi Sasaki

“…TMPRSS2, a human transmembrane protease enzyme, plays a crucial role in the spread of certain viruses, including influenza and coronaviruses. …”
Get full text