Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural p... by Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol

“…Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. …”
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CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE by Ali Mazloum, N. G. Zinyakov, A. S. Igolkin, N. N. Vlasova

“…Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. …”
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RAREFAN: A webservice to identify REPINs and RAYTs in bacterial genomes by Fortmann-Grote, Carsten, Irmer, Julia von, Bertels, Frederic

“…Yet, there is at least one class of persistent prokaryotic mobile genetic elements: REPINs. REPINs are non-autonomous transposable elements replicated by single-copy transposases called RAYTs. …”
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N7-methylguanosine modification in cancers: from mechanisms to therapeutic potential by Qihui Wu, Xiaodan Fu, Guoqian Liu, Xiaoyun He, Yimin Li, Chunlin Ou

“…Abstract N7-methylguanosine (m7G) is an important RNA modification involved in epigenetic regulation that is commonly observed in both prokaryotic and eukaryotic organisms. Their influence on the synthesis and processing of messenger RNA, ribosomal RNA, and transfer RNA allows m7G modifications to affect diverse cellular, physiological, and pathological processes. m7G modifications are pivotal in human diseases, particularly cancer progression. …”
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Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus. by Hannah Ochner, Jan Böhning, Zhexin Wang, Abul K Tarafder, Ido Caspy, Tanmay A M Bharat

“…Comparison of the P. aeruginosa PAO1 T4P with T4P structures from other organisms, both at the level of the pilin subunits and the fully assembled pili, confirms previously described common architectural principles whilst highlighting key differences between members of this abundant class of prokaryotic filaments. This study provides a structural framework for understanding the molecular and cell biology of these important cellular appendages mediating interaction of prokaryotes to surfaces.…”
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Expression of the chloroplast genome: modern concepts and experimental approaches by M. G. Siniauskaya, N. G. Danilenko, N. V. Lukhanina, A. M. Shymkevich, O. G. Davydenko

“…The cpDNA expression retains prokaryotic features, cotranscription in the operon, bacteria-like RNA polymerases and promoters, 70S ribosomes etc., also new characters appear such as uncoupling of transcription with translation, phage-type RNA polymerases, RNA editing, and splicing of primary transcripts. …”
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Soft Lithography and Minimally Human Invasive Technique for Rapid Screening of Oral Biofilm Formation on New Microfabricated Dental Material Surfaces by Marta Alvarez-Escobar, Sidónio C. Freitas, Derek Hansford, Fernando J. Monteiro, Alejandro Pelaez-Vargas

“…Wide information is available about cell/biomaterial interactions using eukaryotic and prokaryotic cells on surfaces of dental materials with different topographies, but studies using human being are still limited. …”
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Comparison of Oral Microbe Quantities from Tongue Samples and Subgingival Pockets by André Göhler, Stefanie Samietz, Carsten Oliver Schmidt, Thomas Kocher, Ivo Steinmetz, Birte Holtfreter

“…To improve understanding of periodontitis pathology, we need more profound knowledge of relative abundances of single prokaryotic species and colonization dynamics between habitats. …”
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Expression evaluation of exogenous and endogenous alcohol dehydrogenase genes in transgenic Arabidopsis by Haoqiang Yu, Hong Lv, Fengzhong Lu, Qingqing Yang, Huaming Duan, Wanchen Li, Fengling Fu

“…To detect the differences in expression, protein accumulation, and enzyme activity between transformed exogenous and endogenous genes, the coding sequences (CDSs) of the alcohol dehydrogenase (ADH) genes were cloned from dicotyledonous Arabidopsis, monocotyledonous maize, and prokaryotic Escherichia coli, constructed into expression vector pBI121-cMycNY, and used to transform wild-type Arabidopsis, respectively. …”
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ADATs: roles in tRNA editing and relevance to disease by Mao Xue-Ling, Eriani Gilbert, Zhou Xiao-Long

“…The most studied example is the TadA- or ADAT2/3-mediated A-to-I conversion of the tRNA wobble position in the anticodon of prokaryotic or eukaryotic tRNAs, respectively. This review provides detailed information on A-to-I and C-to-U editing of tRNAs in different domains of life, presents recent new findings on ADATs for DNA editing, and finally comments on the association of mutations in the ADAT3 gene with intellectual disability.…”
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Optimization and Validation of Indirect ELISA Using Truncated TssB Protein for the Serodiagnosis of Glanders amongst Equines by Harisankar Singha, Praveen Malik, Sachin K. Goyal, Sandip K. Khurana, Chiranjay Mukhopadhyay, Vandana K. Eshwara, Raj K. Singh

“…In an attempt to develop recombinant protein based enzyme-linked immunosorbent assay (ELISA), N-terminal 200 amino acid sequences of B. mallei TssB protein—a type 6 secretory effector protein—were expressed in prokaryotic expression system. Diagnostic potential of recombinant TssB protein was evaluated in indirect ELISA using a panel of glanders positive (n=49), negative (n=30), and field serum samples (n=1811). …”
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Genomic Variability among Field Isolates and Laboratory-Adapted Strains of Leptospira borgpetersenii Serovar Hardjo by Alejandro Llanes, Carlos Mario Restrepo, Pablo Riesgo-Ferreiro, Sreekumari Rajeev

“…In addition, the bioinformatics methodology used in this study for variant calling can be useful to other groups working with nonmodel prokaryotic organisms such as Leptospira species.…”
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Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus by George Tetz, Kristina Kardava, Maria Vecherkovskaya, Alireza Khodadadi-Jamayran, Aristotelis Tsirigos, Victor Tetz

“…This system is composed of DNA- and RNA-based Teazeled receptors (TezRs) found on the surface of prokaryotic and eukaryotic cells, as well as integrases and recombinases. …”
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GENOME POLITICS: STRATEGY AND TACTICS by Jonas Rubikas

“…This is clearly seen when the comparison between the information content (the gene number) and the life cycle of small genomes (plasmids, viruses, singlecellular prokaryotic organisms) is made: large space is occupied by information on replication enzymes and the sequence of replication origin. …”
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Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects by Juliana Costa Gaspar, Lucas Santos de Santana, Camila Menezes Freire de Souza, Marcos Montani Caseiro, Rosane Rezende de Souza Giuliani, Cleide Barbieri de Souza

“…For this reason, the EZH2 gene was isolated from peripheral blood genomic DNA from CML patients and cloned in prokaryotic systems. Therefore, in this study, we proceed, defined and proved the interspe- cies gene transfer procedures and discussed the future of biotechnology in researches and treatments, especially in CML.…”
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pH-induced conformational changes in the selectivity filter of a potassium channel lead to alterations in its selectivity and permeation properties by Carlos Coll-Díez, Ana Marcela Giudici, Alberto Potenza, José Manuel González-Ros, José Antonio Poveda

“…Here we studied the KirBac1.1 channel, a prokaryotic analog of inwardly rectifying K+ channels, confronted to permeant (K+) and non-permeant (Na+) cations. …”
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Detection of CRISPR cassettes and cas genes in the Arabidopsis thaliana genome by Yu. M. Konstantinov, I. S. Petrushin

“…The discovery of CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins), which implement the adaptive immunity function in prokaryotes, raises the question whether such a mechanism of antiviral protection could be caught up by evolution and used by representatives of eukaryotes (in particular, plants). …”
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Comparative diagnostic performance of recombinant Tams1 protein based dot-ELISA in detecting tropical theileriosis in naturally infected cattle by Sanjhi Paliwal, Vikrant Sudan, Daya Shanker

“…The recombinant Tams1 protein was successfully cloned and expressed using a pET-30b (+) expression vector in a prokaryotic system. The protein was purified with Ni-NTA chromatography, confirmed for immunoreactivity with T. annulata positive serum via Western blot analysis, and used to optimize both dot-ELISA and plate-ELISA. …”
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Ag(I) camphorimine complexes with antimicrobial activity towards clinically important bacteria and species of the Candida genus. by João M S Cardoso, Soraia I Guerreiro, Andreia Lourenço, Marta M Alves, M Fátima Montemor, Nuno P Mira, Jorge H Leitão, M Fernanda N N Carvalho

“…These distinct results suggest that the complexes may have different mechanisms against prokaryotic and eukaryotic cells. The antifungal activity of the Ag(I) camphorimine complexes (in particular of complex 1) was found to be very high (MIC = 2 μg/mL) against C. parapsilosis, being also registered a prominent activity against C. tropicalis and C. glabrata. …”
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Taxonomic refinement of Bacillus thuringiensis by Nagham Shiekh Suliman, Reza Talaei-Hassanloui, Hamid Abachi, Sadegh Zarei, Ebrahim Osdaghi

“…WGS-based phylotaxonomic investigations showed that out of 885 strains 803 strains authentically belonged to B. thuringiensis while 82 strains were mislabeled as B. thuringiensis having dDDH and ANI values less than the acceptable threshold of 70 and 95% respectively, for prokaryotic species definition in comparison with the B. thuringiensis type strain. …”
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