Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure by Rahul H.M. Pires, Maria P. Tomasino, Stéphane Simon, Rémy Buzier, Gilles Guibaud, C. Marisa R. Almeida, Ana P. Mucha

“…HTS and qPCR revealed changes, particularly in archaea, with reduced relative abundance at higher TE concentrations. Shifts in prokaryotic communities suggest alterations in AD metabolic pathways. …”
Get full text

mSphere of Influence: How the single cell contributes to the collective by Gina R. Lewin

“…In this mSphere of Influence article, she reflects on how two papers describing bacterial single-cell RNA-seq—“Prokaryotic single-cell RNA sequencing by in situ combinatorial indexing” by S. …”
Get full text

Structure and metabolic function of spatiotemporal pit mud microbiome by Zhihua Li, Chi Zhao, Zhenyu Mao, Fengju Zhang, Ling Dong, Chuan Song, Yao Chen, Xin Fu, Zonghua Ao, Yanfei Xiong, Qin Hui, Weizhi Song, Petri Penttinen, Suyi Zhang

“…Furthermore, we identified relationships between prokaryotes and 29 carboxyl metabolites, including medium- and long-chain fatty acids. …”
Get full text

Long-term effects of combining anaerobic digestate with other organic waste products on soil microbial communities by Daniela Mora-Salguero, Denis Montenach, Manon Gilles, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau, Sophie Sadet-Bourgeteau

“…The fertilization strategies moderately influenced prokaryotic and fungal community structures, with distinct patterns depending on the OWP and nitrogen source. …”
Get full text

The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria by Meina Neumann, Silke Leimkühler

“…Biogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. …”
Get full text

Structural basis for metal ion transport by the human SLC11 proteins DMT1 and NRAMP1 by Márton Liziczai, Ariane Fuchs, Cristina Manatschal, Raimund Dutzler

“…Their structures, while sharing general properties with known prokaryotic homologs, display distinct features that lead to stronger transition metal ion selectivity.…”
Get full text

Protein Machineries Involved in the Attachment of Heme to Cytochrome c: Protein Structures and Molecular Mechanisms by Carlo Travaglini-Allocatelli

“…To accomplish this task, prokaryotic and eukaryotic cells have evolved distinctive protein machineries composed of different proteins. …”
Get full text

Unveiling microbial succession dynamics on different plastic surfaces using WGCNA. by Keren Davidov, Sheli Itzahri, Liat Anabel Sinberger, Matan Oren

“…We employed long-read 16S rRNA metabarcoding to profile the prokaryotic microbiome's taxonomic composition at five time points throughout the experiment. …”
Get full text

The highly rugged yet navigable regulatory landscape of the bacterial transcription factor TetR by Cauã Antunes Westmann, Leander Goldbach, Andreas Wagner

“…This in-depth analysis of a prokaryotic gene regulator reveals a landscape that is navigable but much more rugged than the landscapes of eukaryotic regulators.…”
Get full text

The symbiotic origin of the eukaryotic cell by López-García, Purificación, Moreira, David

“…Here, we review the recent discoveries that led to this conceptual shift, briefly evoking current models of eukaryogenesis and the challenges ahead to discriminate between them and to establish a detailed, plausible scenario that accounts for the evolution of eukaryotic traits from those of their prokaryotic ancestors.…”
Get full text

Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform by Aleksandra Steć, Andrea Heinz, Szymon Dziomba

“…Extracellular vesicles (EVs) are a part of a cell-to-cell communication system of prokaryotic and eukaryotic organisms. Their ability to penetrate biological barriers and to transfer molecules between cells shows their potential as a novel class of drug delivery platform. …”
Get full text

Acholeplasma laidlawii PG8 Culture Adapted to Unfavorable Growth Conditions Shows an Expressed Phytopathogenicity by Vladislav M. Chernov, Natalia E. Moukhametshina, Yurii V. Gogolev, Tatiana N. Nesterova, Maxim V. Trushin, Olga A. Chernova

“…Mycoplasmas are the smallest, self-replicating, prokaryotic organisms with avid biochemical potential and spreading in higher eukaryotes in nature. …”
Get full text

Transmission Electron Microscopy Studies of Cellular Responses to Entry of Virions: One Kind of Natural Nanobiomaterial by Zheng Liu, Shuyu Liu, Jinming Cui, Yurong Tan, Jian He, Jingqiang Zhang

“…The bacteriophage-bacteria interactions are also introduced to elucidate how the bacteriophage conquers the barrier of cell walls in the prokaryotic cells to transport genome into the host.…”
Get full text

Application of Sequence-Dependent Electrophoresis Fingerprinting in Exploring Biodiversity and Population Dynamics of Human Intestinal Microbiota: What Can Be Revealed? by Geert Huys, Tom Vanhoutte, Peter Vandamme

“…The success of the SDE technology lays in the fact that it allows visualization of the predominant members of complex microbial ecosystems independent of their culturability and without prior knowledge on the complexity and diversity of the ecosystem. Mainly using the prokaryotic 16S rRNA gene as PCR amplification target, SDE-based community fingerprinting turned into one of the leading molecular tools to unravel the diversity and population dynamics of human intestinal microbiota. …”
Get full text

MsDpo4—a DinB Homolog from Mycobacterium smegmatis—Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches by Amit Sharma, Deepak T. Nair

“…Error-prone DNA synthesis in prokaryotes imparts plasticity to the genome to allow for evolution in unfavorable environmental conditions, and this phenomenon is termed adaptive mutagenesis. …”
Get full text

Evolution by natural selection is a scientific law and not just a theory by Daniel J.M. Crouch, Walter F. Bodmer

“…The developing knowledge of prokaryotic microorganisms and the discovery of DNA or RNA as the fundamental basis of inheritance in all living organisms, together with Mendel’s laws, now provide the four basic conditions for evolution by natural selection, namely reproduction that is stable, but allows for variation that can increase fitness in the broadest sense. …”
Get full text

Distribution of bacterial DNA repair proteins and their co-occurrence with immune systems by Sumanth K. Mutte, Patrick Barendse, Pilar Bobadilla Ugarte, Daan C. Swarts

“…Furthermore, we report co-occurrences of DNA repair proteins with immune systems, including specific CRISPR-Cas subtypes, prokaryotic Argonautes (pAgos), dGTPases, GAPS2, and Wadjet. …”
Get full text

The Transcriptional Network Structure of a Myeloid Cell: A Computational Approach by Jesús Espinal-Enríquez, Daniel González-Terán, Enrique Hernández-Lemus

“…We compare these results with those of RegulonDB, a highly curated transcriptional network for the prokaryotic organism E. coli, finding similarities between general hallmarks on both transcriptional programs. …”
Get full text

Selection and characterization of specific nanobody against bovine virus diarrhea virus (BVDV) E2 protein. by Tiansen Li, Meiling Huang, Hongran Xiao, Guoqi Zhang, Jinhua Ding, Peng Wu, Hui Zhang, Jinliang Sheng, Chuangfu Chen

“…We obtained a BVDV-E2 antigen epitope bait protein by prokaryotic expression using the nucleotide sequence of the E2 gene of the BVDV-NADL strain published in GenBank. …”
Get full text

CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE by Ali Mazloum, N. G. Zinyakov, A. S. Igolkin, N. N. Vlasova

“…Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. …”
Get full text