HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model by Yi-fu He, Xi-min Hu, Md. Asaduzzaman Khan, Bo-yao Yu, Yi-cun Sheng, Xian-zhong Xiao, Xin-xing Wan, Si-pin Tan, Kun Xiong

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MyVivarium: A cloud-based lab animal colony management application with realtime ambient sensing by Robinson Vidva, Mir Abbas Raza, Jaswant Prabhakaran, Ayesha Sheikh, Alaina Sharp, Hayden Ott, Amelia Moore, Christopher Fleisher, Hailey Netherton, Evan Goldstein, Pothitos M. Pitychoutis, Tam V. Nguyen, Aaron Sathyanesan

“…Management of research-animal colonies is vital for lab productivity in preclinical research. Labs often rely on inefficient paper-based or spreadsheet-based methods to manage animal colonies. …”
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Generation of a genetically engineered porcine melanoma model featuring oncogenic control through conditional Cre recombination by Dongjin Oh, Nayoung Hong, Kiyoung Eun, Joohyeong Lee, Lian Cai, Mirae Kim, Hyerin Choi, Ali Jawad, Jaehyung Ham, Min Gi Park, Bohye Kim, Sang Chul Lee, Changjong Moon, Hyunggee Kim, Sang-Hwan Hyun

“…The novel porcine model provides a valuable tool for elucidating melanoma development and metastasis mechanism, advancing translational medicine, and facilitating preclinical evaluation of new anticancer drugs.…”
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Quantification and correlation of amyloid-β plaque load, glial activation, GABAergic interneuron numbers, and cognitive decline in the young TgF344-AD rat model of Alzheimer’s dise... by Anett Futácsi, Anett Futácsi, Anett Futácsi, Kitti Rusznák, Kitti Rusznák, Gergely Szarka, Gergely Szarka, Gergely Szarka, Béla Völgyi, Béla Völgyi, Ove Wiborg, Boldizsár Czéh, Boldizsár Czéh, Boldizsár Czéh

“…BackgroundAnimal models of Alzheimer’s disease (AD) are essential tools for investigating disease pathophysiology and conducting preclinical drug testing. In this study, we examined neuronal and glial alterations in the hippocampus and medial prefrontal cortex (mPFC) of young TgF344-AD rats and correlated these changes with cognitive decline and amyloid-β plaque load.MethodsWe compared TgF344-AD and non-transgenic littermate rats aged 7–8 months of age. …”
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Beyond metabolic messengers: Bile acids and TGR5 as pharmacotherapeutic intervention for psychiatric disorders by Arief Gunawan Darmanto, Ting-Lin Yen, Jing-Shiun Jan, Tran Thanh Duy Linh, Rajeev Taliyan, Chih-Hao Yang, Joen-Rong Sheu

“…These include dietary adjustments to enhance beneficial bile acids production and the use of specific TGR5 agonists that have shown promise in preclinical and clinical settings for their regulatory effects on critical pathways such as cAMP-PKA, NRF2-mediated antioxidant responses, and neuroinflammation. …”
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Afatinib and Necitumumab in EGFR-Mutant NSCLC with Acquired Resistance to Tyrosine Kinase Inhibitors by Nathaniel J. Myall, MD, Jennifer G. Whisenant, PhD, Joel W. Neal, MD, PhD, Wade T. Iams, MD, Karen L. Reckamp, MD, Sally York, MD, PhD, Lynne D. Berry, PhD, Yu Shyr, PhD, Leora Horn, MD, Heather A. Wakelee, MD, Sukhmani K. Padda, MD

“…Introduction: Although tyrosine kinase inhibitors (TKIs) are effective against NSCLC harboring sensitizing EGFR gene mutations, acquired resistance is inevitable. Preclinical studies suggest that combining EGFR TKI and monoclonal antibody therapies may have activity in EGFR-mutated NSCLC that has progressed on TKI therapy alone. …”
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The Usage of Lion’s Mane in Sports and Its Metabolic Impact – a literature review by Karolina Stankevič, Kamila Fuczyło, Ewelina Kopczyńska, Piotr Kulej, Wiktor Biesiada, Justyna Weronika Kmieć, Justyna Woźniak, Anna-Mariia Chernysh, Aleksandra Dusińska, Jakub Waszczyński

“…However, direct evidence on its impact on athletic metrics such as endurance, strength, or recovery times is limited. Preclinical studies are promising but need to be validated in well-designed human trials. …”
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Safety and immunogenicity of an optimized self-replicating RNA platform for low dose or single dose vaccine applications: a randomized, open label Phase I study in healthy voluntee... by Christian J. Maine, Shigeki J. Miyake-Stoner, Darina S. Spasova, Gaelle Picarda, Annie C. Chou, Emily D. Brand, Melanie D. Olesiuk, Christine C. Domingo, Hunter J. Little, Thomas T. Goodman, Jacqueline L. Posy, Jasmin Gonzalez, Terrina L. Bayone, Jessica Sparks, Ebony N. Gary, Zhi Xiang, Nicholas J. Tursi, Casey E. Hojecki, Hildegund C. J. Ertl, David B. Weiner, Irafasha C. Casmil, Anna K. Blakney, Brandon Essink, Guillermo Somodevilla, Nathaniel S. Wang, Andrew J. Geall, Zelanna Goldberg, Parinaz Aliahmad

“…Here, we develop an srRNA vaccine platform with optimized non-coding regions and demonstrate immunogenicity and safety in preclinical and clinical development. Optimized srRNA vaccines generate protective immunity (according to the WHO defined thresholds) at doses up to 1,000,000-fold lower than mRNA in female mouse models of influenza and rabies. …”
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Targeted inhibition of Aurora kinase A promotes immune checkpoint inhibition efficacy in human papillomavirus-driven cancers by Pragya Sinha, Faye M Johnson, Soma Ghosh, Jagannadha K Sastry, Madison P O'Hara, Tuhina Mazumdar, Lacin Yapindi

“…Here, we explored the potential of Aurora A inhibition to enhance response to ICIs in immune-competent preclinical models of HPV-driven cancers.Methods We assessed the induction of apoptosis, DNA damage, and immunogenic cell death (ICD) in response to treatment with the Aurora A inhibitor alisertib in vitro and antitumor efficacy of alisertib as a monotherapy and in combination with ICIs that inhibit programmed cell death protein-1 (PD-1) or cytotoxic T-lymphocyte associated protein 4 (CTLA-4) in murine HPV-positive immune-competent tumor models. …”
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Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer by Babak Jahangiri, Zahra-Soheila Soheili, Elahe Asadollahi, Mehdi Shamsara, Vahid Shariati, Alireza Zomorodipour

“…These genesshowed increased expression in TA-MSCs, TNBC cells, and tumor tissues.Conclusion: The findings of the present study revealed a complex interplay between TA-MSCs and TNBC cells thataffects tumor growth and metastasis. Preclinical results indicate that intratumoral IL-12 immunotherapy shows promisein overcoming TA-MSC-promoted tumor growth and metastasis.…”
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The APOE∗3-Leiden Heterozygous Glucokinase Knockout Mouse as Novel Translational Disease Model for Type 2 Diabetes, Dyslipidemia, and Diabetic Atherosclerosis by Marianne G. Pouwer, Suvi E. Heinonen, Margareta Behrendt, Anne-Christine Andréasson, Arianne van Koppen, Aswin L. Menke, Elsbet J. Pieterman, Anita M. van den Hoek, J. Wouter Jukema, Brendan Leighton, Ann-Cathrine Jönsson-Rylander, Hans M. G. Princen

“…There is a lack of predictive preclinical animal models combining atherosclerosis and type 2 diabetes. …”
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Short- and long-term changes in neurological, behavioural, and blood biomarkers following repeated mild traumatic brain injury in rats—potential biological sex-dependent effects by Rodrigo Moraga-Amaro, Oscar Moreno, Jordi Llop, Marion Bankstahl, Marion Bankstahl, Jens P. Bankstahl

“…These sex differences should be considered when translating preclinical biomarker candidates to the clinic. Understanding these differences could guide the diagnosis and treatment of CTE in a personalized manner, offering hope for more effective treatments in the future.…”
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Suppression of epileptic seizures by transcranial activation of K+-selective channelrhodopsin by Xiaodong Duan, Chong Zhang, Yujie Wu, Jun Ju, Zhe Xu, Xuanyi Li, Yao Liu, Schugofa Ohdah, Oana M. Constantin, Yifan Pan, Zhonghua Lu, Cheng Wang, Xiaojing Chen, Christine E. Gee, Georg Nagel, Sheng-Tao Hou, Shiqiang Gao, Kun Song

“…Our approach for transcranial optogenetic inhibition of neural hyperactivity may be adapted for cell type-specific neuromodulation in both basic and preclinical settings.…”
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Angel or devil: the dual roles of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside in the development of liver injury based on integrating pharmacological techniques: a system... by Jiajie Jiang, Jiajie Jiang, Qixiu Wang, Qiang Wu, Bobin Deng, Cui Guo, Jie Chen, Jinhao Zeng, Yaoguang Guo, Xiao Ma

“…The purpose of this study is to evaluate both the hepatoprotective and hepatotoxic effects of TSG and give therapeutic guidance.MethodsThis study performed a systematic search of eight databases to identify preclinical literature up until March 2024. The CAMARADES system evaluated evidence quality and bias. …”
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Antibody-based delivery of interleukin-2 modulates the immunosuppressive tumor microenvironment and achieves cure in pancreatic ductal adenocarcinoma syngeneic mice by Carmine Carbone, Roberto De Luca, Emanuele Puca, Antonio Agostini, Alessia Caggiano, Lorenzo Priori, Annachiara Esposito, Serena Ascrizzi, Geny Piro, Lisa Salvatore, Francesco De Sanctis, Stefano Ugel, Vincenzo Corbo, Dario Neri, Giampaolo Tortora

“…This study investigates the preclinical efficacy of an innovative targeted therapy based on antibody-cytokine fusion proteins, specifically interleukin-2 (IL-2), a pivotal driver of cell-mediated immunity, fused to L19 antibody, which selectively binds to extra domain B of fibronectin (EDB-FN1) expressed in the tumor microenvironment. …”
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Incidence and risk of endocrine and metabolic abnormalities linked to PARP inhibitors in solid tumors: a meta-analysis by Shunlian Fu, Pingjin Zou, Zengyi Fang, Xinxiang Zhou, Junyang Chen, Cuicui Gong, Li Quan, Bing Lin, Qiu Chen, Jinyi Lang, Meihua Chen

“…Abstract Background Poly (ADP-ribose) polymerase inhibitors (PARPi) serve as crucial therapeutic agents in solid tumor treatment. Preclinical investigations suggest a potential protective function of PARPi against endocrine and metabolic impairments. …”
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Efficacy of Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) and Alternaria destruens (Ascomycota: Pleosporaceae) for managing Phenacoccus solenopsis Tinsley (Hemiptera:... by Mohamed El Aalaoui, Said Rammali, Fatima Zahra Kamal, Alin Ciobică, Bouchaib Bencharki, Abdellatif Rahim, Luminita Diana Hritcu, Laura Romila, Vasile Burlui, Mohamed Sbaghi

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Phenolic acids from Anisopus mannii modulates phosphofructokinase 1 to improve glycemic control in patients with type 2 diabetes: A double-blind, randomized, clinical trial by Peter U. Amadi, Justice O. Osuoha, Chidi N. Ekweogu, Suha J. Jarad, Esienanwan E. Efiong, Prince C. Odika, Chioma Ejiofor, Oluchi Aloy-Amadi, Govind S. Gill, Chiamaka W. Adumekwe, Ailun Gaowa, Dawei Zhang, Barbora de Courten, Emmanuel N. Agomuo

“…This herb rescued β-cells from streptozotocin-mediated destruction, thereby improving glycemic control. Supported by the preclinical trial, eighty-five patients with type 2 diabetes (T2D) receiving first-line medications were enrolled in a double-blind, randomized, placebo-controlled trial with a 90 % power level. …”
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PD-1 cis-targeted IL-2v in combination with radiotherapy inhibits lung cancer growth and remodels the immune microenvironment by Pedro J Romero, Sara Labiano, Gregory Verdeil, Christian Klein, Pablo Umana, Tania Wyss, Christine Trumpfheller, Marie-Catherine Vozenin, Céline Godfroid, Jackeline Romero, Vincent Roh, Genrich V Tolstonog, Chia-Hsien Chuang, Andrea Kelemen, Laura Codarri Deak

“…In this regard, we used a preclinical model of metastatic lung adenocarcinoma (SV2-OVA) to assess the safety and efficacy of novel radio-immunotherapy combining hypofractionated radiotherapy (HRT) with muPD1-IL2v immunocytokine and muFAP-CD40 bispecific antibody.Methods We evaluated the changes in the lung immune microenvironment at multiple timepoints following combination therapies and investigated their underlying antitumor mechanisms. …”
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Stabilizing versus Destabilizing the Microtubules: A Double-Edge Sword for an Effective Cancer Treatment Option? by Daniele Fanale, Giuseppe Bronte, Francesco Passiglia, Valentina Calò, Marta Castiglia, Florinda Di Piazza, Nadia Barraco, Antonina Cangemi, Maria Teresa Catarella, Lavinia Insalaco, Angela Listì, Rossella Maragliano, Daniela Massihnia, Alessandro Perez, Francesca Toia, Giuseppe Cicero, Viviana Bazan

“…Here we review several natural and synthetic microtubule-targeting agents, which showed antitumor activity and increased efficacy in comparison to traditional drugs in various preclinical and clinical studies. Cryptophycins, combretastatins, ombrabulin, soblidotin, D-24851, epothilones and discodermolide were used in clinical trials. …”
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