Engineered ipilimumab variants that bind human and mouse CTLA-4 by Brett Robison, SJ Diong, Anusha Kumar, Thomas M. Moon, Olin Chang, Bryant Chau, Christine Bee, Ishita Barman, Arvind Rajpal, Alan J. Korman, Sean West, Pavel Strop, Peter S. Lee

“…Testing of candidate monoclonal antibody therapeutics in preclinical models is an essential step in drug development. …”
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Biomechanical Impact of Vertebral Augmentation Techniques: Clinical and Radiological Results in the Literature by Eliodoro Faiella, Federica Vaccarino, Domiziana Santucci, Elva Vergantino, Bruno Beomonte Zobel, Rosario Francesco Grasso

“…Many of these studies have utilized indirect parameters, such as the Beck Index and kyphotic angles (α and γ) measured by the Cobb method, to evaluate improvements in vertebral deformity. Furthermore, preclinical studies indicate that third-generation devices like SpineJack^® enhance vertebral height restoration and stability, with performance influenced by bone quality and implant positioning, and have demonstrated superior initial and sustained height maintenance compared to kyphoplasty. …”
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Epicardially Placed Bioengineered Cardiomyocyte Xenograft in Immune-Competent Rat Model of Heart Failure by Ikeotunye Royal Chinyere, Pierce Bradley, Joshua Uhlorn, Joshua Eason, Saffie Mohran, Giuliana G. Repetti, Sherry Daugherty, Jen Watson Koevary, Steven Goldman, Jordan J. Lancaster

“…Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are under preclinical investigation as a cell-based therapy for heart failure post-myocardial infarction. …”
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Regional Medical Campuses in Canada by Aaron Johnston, Amanda Bell, Kristy Penner, Trushar Patel, Grace Perez

“…Combined model RMCs, offering both preclinical and clinical education have shown promising results in producing physicians who work in rural and regional settings and are currently a key avenue of expansion of medical training in Canada. …”
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Combination of rapamycin and adipose-derived mesenchymal stromal cells enhances therapeutic potential for osteoarthritis by Damien Veret, Gautier Tejedor, Esther Perez, Alison Chomette, Maylis Farno, Rosanna Ferreira-Lopez, Louis Dagneaux, Yves-Marie Pers, Christian Jorgsensen, Claire Gondeau, Jean-Marc Brondello

“…However, despite the promising regenerative properties documented in preclinical studies, for instance in osteoarthritis (OA), the therapeutic translation of these results in patients has not been fully conclusive. …”
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Clinical efficacy and learning curve of percutaneous endoscopic cervical discectomy for symptomatic cervical spondylotic radiculopathy by Xiao Sun, Lijuan Zhan, Zhongxin Tang, Mingkui Shen, Haijun Ma, Jun Tan

“…However, longer operative times do not negatively impact preclinical outcomes. Trial registration Retrospectively registered.…”
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Enhancing Medical Student Engagement Through Cinematic Clinical Narratives: Multimodal Generative AI–Based Mixed Methods Study by Tyler Bland

“… Abstract BackgroundMedical students often struggle to engage with and retain complex pharmacology topics during their preclinical education. Traditional teaching methods can lead to passive learning and poor long-term retention of critical concepts. …”
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Proximal tubule-on-chip as a model for predicting cation transport and drug transporter dynamics by Isy Petit, Quentin Faucher, Jean-Sébastien Bernard, Perrine Giunchi, Antoine Humeau, François-Ludovic Sauvage, Pierre Marquet, Nicolas Védrenne, Florent Di Meo

“…OoC are particularly relevant at the fundamental and preclinical stages of drug development by providing more accurate assessment of key pharmacokinetic events. …”
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Developing clinical reasoning along the cognitive continuum: a mixed methods evaluation of a novel Clinical Diagnosis Assessment by Lucinda E. Ainge, Amanda K. Edgar, Jacqueline M. Kirkman, James A. Armitage

“…Abstract Background Clinical reasoning is a professional capability required for clinical practice. In preclinical training, clinical reasoning is often taught implicitly, and feedback is focused on discrete outcomes of decision-making. …”
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Pharmacological effects of fibroblast growth factor 21 are sex-specific in mice with the <em>lethal yellow</em> (A^y) mutation by E. N. Makarova, T. V. Yakovleva, N. Yu. Balyibina, K. O. Baranov, E. I. Denisova, A. D. Dubinina, N. A. Feofanova, N. M. Bazhan

“…Sex is an important biological variable that must be considered when conducting preclinical studies; however, in laboratory animal models, the pharmacological effects of FGF21 are well documented only for male mice. …”
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Evolutionary constrained genes associated with autism spectrum disorder across 2,054 nonhuman primate genomes by Yukiko Kikuchi, Mohammed Uddin, Joris A. Veltman, Sara Wells, Christopher Morris, Marc Woodbury-Smith

“…Rhesus macaques (Macaca mulatta) have been extensively used for preclinical neurobiological research because of remarkable similarities to humans across biology and behaviour that cannot be captured by other experimental animals. …”
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The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research by Yun Liu, Ting‐Ting Feng, Wei Tong, Zhi Guo, Xia Li, Qi Kong, Zhi‐Guang Xiang

“…Conclusions The current in vitro viral infection study showed the key innated immunity character of macrophages, which suggested the replacement value of viral infection cells model, to reduce the animal usage in preclinical research.…”
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Antibody-induced erythrophagocyte reprogramming of Kupffer cells prevents anti-CD40 cancer immunotherapy-associated liver toxicity by Florence Vallelian, Marc Pfefferlé, Irina L Dubach, Raphael M Buzzi, Elena Dürst, Nadja Schulthess-Lutz, Livio Baselgia, Kerstin Hansen, Larissa Imhof, Sandra Koernig, Didier Le Roy, Thierry Roger, Rok Humar, Dominik J Schaer

“…Background Agonistic anti-CD40 monoclonal antibodies (mAbs) have emerged as promising immunotherapeutic compounds with impressive antitumor effects in mouse models. However, preclinical and clinical studies faced dose-limiting toxicities mediated by necroinflammatory liver disease. …”
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Acute and Subchronic Toxicity Assessment of Conventional Soxhlet Cymbopogon citratus Leaves Extracts in Sprague–Dawley Rats by Jacob Apibilla Ayembilla, Abdul Raouf Khalid, Sharif Buari Abubakari, Abdul Rashid Adams, Felix Abekah Botchway, Stephen Antwi, Phyllis Naa Yarley Otu, Michael Appiah, George Osei-Adjei, Kwame Owen Kottoh, Peace Ahiabenu-Williams, Olga Quasie

“…To encourage its usage, preclinical validation of the safety profile of the plant is required. …”
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Emerging PET Radiotracers and Targets for Imaging of Neuroinflammation in Neurodegenerative Diseases: Outlook Beyond TSPO by Vidya Narayanaswami PhD, Kenneth Dahl PhD, Vadim Bernard-Gauthier PhD, Lee Josephson PhD, Paul Cumming PhD, Neil Vasdev PhD

“…We provide a brief overview of the cellular expression and function of these targets, noting their selectivity for astrocytes and/or microglia, and highlight the classes of PET radiotracers that have been investigated in early-stage preclinical or clinical research studies of neuroinflammation.…”
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The quality and technological parameters of milk obtained from dairy cows with subclinical mastitis by Magdalena Zalewska, Paulina Brzozowska, Magdalena Rzewuska, Ewelina Kawecka-Grochocka, Daria M. Urbańska, Tomasz Sakowski, Emilia Bagnicka

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AKR1D1*36 C>T (rs1872930) allelic variant is associated with variability of the CYP2C9 genotype predicted pharmacokinetics of ibuprofen enantiomers – a pilot study in healthy volun... by Kapedanovska Nestorovska Aleksandra, Jakjovski Krume, Naumovska Zorica, Sterjev Zoran, Geskovska Nadica Matevska, Mladenovska Kristina, Suturkova Ljubica, Dimovski Aleksandar

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Evaluation of Intraperitoneal [18F]-FDOPA Administration for Micro-PET Imaging in Mice and Assessment of the Effect of Subchronic Ketamine Dosing on Dopamine Synthesis Capacity by Els F. Halff, Sridhar Natesan, David R. Bonsall, Mattia Veronese, Anna Garcia-Hidalgo, Michelle Kokkinou, Sac-Pham Tang, Laura J. Riggall, Roger N. Gunn, Elaine E. Irvine, Dominic J. Withers, Lisa A. Wells, Oliver D. Howes

“…This enables a direct comparison between preclinical and clinical output values. However, chronic intravenous catheters are technically difficult to maintain for longitudinal studies. …”
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Mechanisms of vagus nerve stimulation for the treatment of neurodevelopmental disorders: a focus on microglia and neuroinflammation by Makenna Gargus, Benneth Ben-Azu, Benneth Ben-Azu, Antonia Landwehr, Jaclyn Dunn, Joseph P. Errico, Marie-Ève Tremblay, Marie-Ève Tremblay

“…., modulation of microglia and various neurotransmitter pathways, as well as emerging preclinical and clinical evidence supporting VNS’s impact on symptoms associated with neurodevelopmental disorders, such as those related to CNS inflammation induced by infections. …”
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Raw Cured Poultry Meat Fortified with Bee Pollen: Biomedical Research on Laboratory Animals by Maksim A. Sukhov, Tatiana M. Giro, Sergey V. Kozlov, Irina V. Ziruk

“…However, the rats had normal live weight gain, and their liver demonstrated no histological deviations. In this preclinical study, bee pollen as part of jerky meat formulation had no negative effect on laboratory rats. …”
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