Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma by Hai-Peng Wei, Song Zhan, Qing-An Zhu, Zhen-Juan Chen, Xian Feng, Jun-Yuan Chen, Qi-Lin Zhang, Jingjie Zhao, Lingzhang Meng

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Unbiased identification of cell identity in dense mixed neural cultures by Sarah De Beuckeleer, Tim Van De Looverbosch, Johanna Van Den Daele, Peter Ponsaerts, Winnok H De Vos

“…However, the variability between individual iPSC lines and the lack of efficient technology to comprehensively characterize iPSC-derived cell types hinder its adoption in routine preclinical screening settings. To facilitate the validation of iPSC-derived cell culture composition, we have implemented an imaging assay based on cell painting and convolutional neural networks to recognize cell types in dense and mixed cultures with high fidelity. …”
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Development and validation of an LC-MS/MS method for the simultaneous estimation of tadalafil and macitentan in rat plasma: Greenness assessment and design of experiment approach by Sravanthi Gandu, Kumaraswamy Gandla, Lalitha Repudi

“…This validated LC-MS/MS method provides a robust and sustainable analytical solution for preclinical pharmacokinetic studies, delivering reliable data to inform safe and effective dosing of tadalafil and macitentan.…”
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Protocol for a first-in-human feasibility study of T regulatory cells (TR004) for inflammatory bowel disease using (ex vivo) Treg expansion (TRIBUTE) by Jennifer Clough, Peter Irving, A Toby Prevost, Graham M Lord, Joana Vasconcelos, James B Canavan, Beverley Rodger, D Macallan

“…In vitro-expanded ‘thymus-derived’ regulatory T cells (tTreg) have shown promise in preclinical models of IBD, leading to interest in their use as a potential therapy in CD. …”
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Factors Affecting Performance in Clinical Practice among Preservice Diploma Nursing Students in Northern Tanzania by Helena Marco Gemuhay, Albino Kalolo, Robert Mirisho, Beatrice Chipwaza, Elijah Nyangena

“…Student’s factors and placement based factors played an important role to influence clinical learning experiences. Offering preclinical orientation, distributing and clarifying clinical learning objectives to students, and frequent visits and supervision of students in clinical area may improve student learning experience in clinical placement. …”
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Inflammation and Immune Escape in Ovarian Cancer: Pathways and Therapeutic Opportunities by Liu C, Yin Q, Wu Z, Li W, Huang J, Chen B, Yang Y, Zheng X, Zeng L, Wang J

“…COX-2 inhibitors, Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway blockers, and NF-kB inhibitors have shown potential in preclinical studies, while immune checkpoint inhibitors targeting PD-1/PD-L1 and CTLA-4 have been explored with mixed results in OC. …”
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Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma by Yuxuan Zhang, Jie Liu, Yunzhao Mo, Zetao Chen, Taoliang Chen, Yan Li, Yaofeng Zheng, Shaokang Deng, Xiangdong Xu, Huajian Chen, Haoqi He, Jiansheng Chen, Tao Jin, Xinlin Sun, Yiquan Ke, Jihui Wang

“…The limited passage and self-differentiation abilities of MSCs in vitro hinder preclinical studies on them. In this study, we focused on the safety of immortalized mesenchymal stem cells (im-MSCs) and, for the first time, studied the feasibility of im-MSCs as candidates for the treatment of glioma. …”
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Probiotic Therapy as an Adjuvant in the Treatment of Periodontal Disease: An Innovative Approach by Liliana Sachelarie, Ioana Scrobota, Ioana Romanul, Raluca Iurcov, Georgiana Ioana Potra Cicalau, Liana Todor

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Molecular Mechanisms of Neurodegeneration Related to C9orf72 Hexanucleotide Repeat Expansion by Mirjana Babić Leko, Vera Župunski, Jason Kirincich, Dinko Smilović, Tibor Hortobágyi, Patrick R. Hof, Goran Šimić

“…Although the exact molecular mechanisms through which C9orf72 repeat expansions account for neurodegeneration have not been elucidated, some potential therapeutics, such as antisense oligonucleotides targeting hexanucleotide GGGGCC repeats in mRNA, were successful in preclinical trials and are awaiting phase 1 clinical trials. …”
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Soybean and avocado unsaponifiables: a review of their potential use in the treatment of osteoarthritis by Ana Sabucedo-Suárez, Ana Sabucedo-Suárez, Mónica López-Peña, Mónica López-Peña, María Permuy, María Permuy, Fernando Muñóz, Fernando Muñóz

“…Two reviewers independently screened each article using inclusion and exclusion criteria and categorized the studies into in vitro, preclinical, and clinical groups. According to in vitro research, ASU affect the regulation of molecules related to OA, increasing structural elements like collagen and aggrecan and decreasing pro-inflammatory mediators. …”
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Nanoparticle accumulation and penetration in 3D tumor models: the effect of size, shape, and surface charge by Pierre Cybulski, Maria Bravo, Maria Bravo, Jim Jui-Kai Chen, Indra Van Zundert, Sandra Krzyzowska, Farsai Taemaitree, Hiroshi Uji-i, Hiroshi Uji-i, Hiroshi Uji-i, Johan Hofkens, Johan Hofkens, Susana Rocha, Beatrice Fortuni

“…Preclinical studies have demonstrated that nanoparticles (NPs) hold significant potential for advancing cancer therapy by enhancing therapeutic efficacy while reducing side effects. …”
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Fractionated radiotherapy initiated at the early stage of bone metastasis is effective to prolong survival in mouse model by Yun Zhang, Zhunyi Gao, Ziwei Qi, Jiahe Xu, Jiao Xue, Lujie Xiong, Junhui Wang, Yuhui Huang, Songbing Qin

“…Therefore, these findings provide preclinical evidence to support the application of fractionated RT to treat patients with bone metastasis as earlier as possible.…”
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Evaluation of intranasal TLR2/6 agonist INNA-051: safety, tolerability and proof of pharmacology by Francesca A. Mercuri, Scott White, Hayley A. McQuilten, Charlotte Lemech, Stephan Mynhardt, Rana Hari, Ping Zhang, Nicole Kruger, Grant McLachlan, Bruce E. Miller, Nicholas P. West, Ruth Tal-Singer, Christophe Demaison

“…Conclusions The safety and pharmacology profile of INNA-051 confirms preclinical studies. INNA-051 increased expression of genes and pathways associated with host defence responses against influenza and was associated with a shorter duration of infection. …”
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Pathological roles of ubiquitination and deubiquitination systems in sepsis-induced myocardial dysfunction by Zhiping Wang, Simiao Sun, Lili Huang, Xinlong Chen, Huifen Xu, Hongwei Ma, Mingbing Xiao, Linhua Wang

“…Strategies aimed at modulating E3 ligase activity or restoring DUB balance have shown promise in preclinical studies. This review summarizes current findings on the roles of ubiquitination and deubiquitination in SIMD pathogenesis, highlights the key challenges in advancing this field, and proposes directions for future research. …”
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Bergenin, a bioactive flavonoid: advancements in the prospects of anticancer mechanism, pharmacokinetics and nanoformulations by Pratibha Pandey, Pratibha Pandey, Sorabh Lakhanpal, Danish Mahmood, Han Na Kang, Byunggyu Kim, Sojin Kang, Jinwon Choi, Seungjoon Moon, Shivam Pandey, Suhas Ballal, Sanjay Kumar, Fahad Khan, Bonglee Kim

“…Therefore, our review aimed to update the multifaceted benefits of bergenin to the future scientists and researchers, which can be leveraged to formulate safer and novel cancer therapies, while also establishing a robust framework for future investigations into bergenin in cancer treatment. More preclinical and clinical investigations are needed to validate the candidature of bergenin as a potent anticancer agent.…”
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Circulating Th1, Th2, Th17, Treg, and PD-1 Levels in Patients with Brucellosis by Rongjiong Zheng, Songsong Xie, Qiong Zhang, Ling Cao, Shaniya Niyazi, Xiaobo Lu, Lihua Sun, Yan Zhou, Yuexin Zhang, Kai Wang

“…These results indicate that peripheral T lymphocyte immunity was involved in patients with brucellosis and represents a target for the preclinical and clinical assessment of novel immunomodulating therapeutics. …”
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Learning by making – student-made models and creative projects for medical education: systematic review with qualitative synthesis by Tan Yong Yi, Panwar Shreyans, Ranganath Vallabhajosyula

“…One notable approach involves engaging students in producing creative projects to complement the learning of preclinical topics. A systematic review was conducted to characterize the impact of creative project-based learning on metacognition and knowledge gains in medical students. …”
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Passive infusion of an S2-Stem broadly neutralizing antibody protects against SARS-CoV-2 infection and lower airway inflammation in rhesus macaques. by Christopher T Edwards, Kirti A Karunakaran, Elijah Garcia, Nathan Beutler, Matthew Gagne, Nadia Golden, Hadj Aoued, Kathryn L Pellegrini, Matthew R Burnett, Christopher Cole Honeycutt, Stacey A Lapp, Thang Ton, Mark C Lin, Amanda Metz, Andrei Bombin, Kelly Goff, Sarah E Scheuermann, Amelia Wilkes, Jennifer S Wood, Stephanie Ehnert, Stacey Weissman, Elizabeth H Curran, Melissa Roy, Evan Dessasau, Mirko Paiardini, Amit A Upadhyay, Ian N Moore, Nicholas J Maness, Daniel C Douek, Anne Piantadosi, Raiees Andrabi, Thomas R Rogers, Dennis R Burton, Steven E Bosinger

“…Collectively, these data demonstrate the protective efficiency of broadly neutralizing S2-targeting antibodies against SARS-CoV-2 infection within the lower airway while providing critical preclinical work necessary for the development of pan-β-CoV vaccines.…”
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Enhancing immunotherapy efficacy with synergistic low-dose radiation in metastatic melanoma: current insights and prospects by Zahid Rafiq, Mingyo Kang, Hampartsoum B. Barsoumian, Gohar S. Manzar, Yun Hu, Carola Leuschner, Ailing Huang, Fatemeh Masrorpour, Weiqin Lu, Nahum Puebla-Osorio, James W. Welsh

“…We meticulously examine the preclinical evidence and ongoing clinical trials, throwing light on the promising prospects of LDRT as a complementary therapy in melanoma treatment. …”
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A Study on Diagnostic Accuracy of Dipsi Method Over Traditional Oral Glucose Tolerance Test Among Antenatal Women by Vijayadevi Shanmugam, Dharani Sudha Gopalraj, Shruthi Nanjudappan, Rajeswari Ravindran

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