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161
REAL-TIME PCR OPTIMIZATION FOR LISTERIA MONOCYTOGENES GENOME DETECTION
Published 2018-10-01“…L. monocytogenes bacteria genome detection technique using real-time polymerase chain reaction (qRT-PCR) was improved. The amplification target was a highly specific and suitable for qualification of all strains iap gen, coding L. monocytogenes р60 surface protein. …”
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162
Nonconvulsive Status Epilepticus Complicating Epstein-Barr Virus Encephalitis in a Child
Published 2014-01-01“…We report a 4-year-old girl with seizure and behavioral changes in whom the analysis of cerebrospinal fluid by polymerase chain reaction was positive for Epstein-Barr virus. …”
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163
Quantitative dynamic evaluation HBsAg in blood in comparison to viral load level in patients with chronic HBV-monoinfection
Published 2011-06-01“…Dynamic assessment of HBV viremia level by polymerase chain reaction and quantitative assessment of blood HBsAg by chemiluminescence was carried out.Results. …”
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164
Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
Published 2002-01-01“…Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. …”
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165
New Diagnostics for Fungal Infections in Transplant Infectious Disease: A Systematic Review
Published 2025-01-01“…In addition, we provide future research directions to substantiate and appropriately utilize these platforms in clinical practice. Directed polymerase chain reaction testing and targeted metagenomic sequencing are being used clinically and show the most promise, though only in conjunction with conventional methods at this time. …”
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166
An epidemiological study of malaria parasites among long–tailed macaques (Macaca fascicularis), pig–tailed macaques (Macaca nemestrina) and silver–leaf monkeys (Trachypithecus cris...
Published 2025-01-01“…Methods: Infections with Plasmodium spp. were determined morphologically from the blood smears which were stained with Giemsa solution and molecularly through nested polymerase chain reaction (PCR) in DNA samples from 68 primates, which were captured at three locations: Jambi (Bungo district), Bengkulu (Muko-Muko district), and Riau Islands (Lingga district). …”
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167
The utilization of microbial cell-free DNA next-generation sequencing for the detection of human herpesvirus-8 in a quaternary care center
Published 2025-01-01“…Results: The clinical and laboratory turnaround times of mcfDNA NGS were 3 and 1 days, respectively (8.5 and 7 days for immunohistochemistry vs 5.5 and 2 days for serum HHV8 polymerase chain reaction). Eight patients received HHV8-related diagnoses, while two had unrelated conditions. …”
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168
Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR
Published 1995-01-01“…Therefore, a polymerase chain reaction (PCR) aided analysis of cytokine gene expression by PBMC or T cells is a valuable tool. …”
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169
Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
Published 2015-01-01“…The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. …”
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170
Infrarenal Aorta Thrombosis Associated with H1N1 Influenza A Virus Infection
Published 2016-01-01“…The patient was tested for pandemic influenza A (H1N1) virus using polymerase chain reaction (PCR) tests, which were positive. …”
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171
A Case of Cutaneous Leishmaniasis Presenting to the Emergency Department
Published 2025-02-01“…The diagnosis was confirmed by the Center for Disease Control and Prevention (CDC) through a polymerase chain reaction (PCR) sample from the presenting skin lesion. …”
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172
Molecular detection of pore-forming leuko toxin in methicillin resistant Staphylococcus aureus isolated from skin infection
Published 2024-01-01“…Antibiotic susceptibility patterns, mecA, 16sRNA, and PVL genes were isolated and detected by polymerase chain reaction (PCR). Results: All isolates were determined to be resistant to cefoxitin discs and oxacillin by using phenotypic analysis, and a genotypic investigation revealed that 79.16% of them carried the mecA gene. …”
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173
Identification of pathogenic bacteria by biosensors in water and wastewater
Published 2024-11-01“…Therefore, timely diagnosis of these pathogens is very important. the use of specialized personnel, machines, and tools in molecular methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) are expensive, and traditional methods such as plate culture are time-consuming. …”
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174
Q Fever Presenting as Recurrent, Culture-Negative Endocarditis with Aortic Prosthetic Valve Failure: A Case Report and Review of the Literature
Published 2006-01-01“…Serum antibodies against Coxiella burnetii were strongly positive, and C burnetii DNA was detected by polymerase chain reaction from his resected aortic valve tissue. …”
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175
Fecal microbiota changes associated with pathogenic and non-pathogenic diarrheas in foals
Published 2025-01-01“…Results Foal diarrhea samples tested positive or negative for common equine neonatal diarrhea pathogens by diagnostic polymerase chain reaction (PCR), which allowed for samples to be segregated as pathogenic or non-pathogenic. …”
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176
New Insight into Laboratory Tests and Imaging Modalities for Fast and Accurate Diagnosis of COVID-19: Alternative Suggestions for Routine RT-PCR and CT—A Literature Review
Published 2020-01-01“…Nowadays, real-time polymerase chain reaction (RT-PCR) and computed tomography (CT) have been frequently utilized in the clinic. …”
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177
miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2
Published 2022-01-01“…The rat model of abdominal aortic constriction was constructed, the changes of myocardial morphology were observed by hematoxylin-eosin (HE) staining, and the hypertrophy-related marker molecules were detected by quantitative real-time polymerase chain reaction (qRT-PCR). At the cellular level, phenylephrine and angiotensin II were added to induce cardiomyocyte hypertrophy. …”
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178
A Practical Review of Cytomegalovirus in Gastroenterology and Hepatology
Published 2019-01-01“…Additionally, liver allograft dysfunction is a significant consequence of CMV infection in liver transplant patients. While polymerase chain reaction and immunohistochemistry techniques allow for the reliable and accurate detection of CMV in the human host, the diagnostic value of different serologic, endoscopic, and histologic tests depends on a variety of factors. …”
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179
Laboratory Diagnosis of Mumps in a Partially Immunized Population: The Nova Scotia Experience
Published 2009-01-01“…The present study describes the performance characteristics of reverse transcriptase polymerase chain reaction (RT-PCR) on buccal and urine specimens and immunoglobulin M (IgM) serology in this partially immune population.…”
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180
Ramsay Hunt Syndrome Associated with Central Nervous System Involvement in an Adult
Published 2016-01-01“…Varicella zoster virus was detected in the cerebrospinal fluid by polymerase chain reaction. She was diagnosed with Ramsay Hunt syndrome associated with spread to the central nervous system. …”
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