Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Chan... by Mohamed A. Lebdah, Amal A. M. Eid, Reham M. ElBakrey, Abd Elgalil. El-Gohary, Mohamed G. Seadawy, Mohamed R. Mousa, Hagar F. Gouda, Nehal I. A. Goda, Mostafa F. El-Hosseny, Ahmed S. El-tahlawy, Rokayya Sami, Rasha A. Al-Eisa, Sarah S. Helal

“…GPV was detected in ducks using polymerase chain reaction, with the highest percentage of positive detection in the bursa of Fabricius (76%). …”
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Sibling Species Composition and Susceptibility Status of Anopheles gambiae s.l. to Insecticides Used for Indoor Residual Spraying in Eastern Uganda by Julius Iga, Stephen Ochaya, Richard Echodu, Elizabeth A. Opiyo, Alex K. Musiime, Angella Nakamaanya, Geoffrey M. Malinga

“…Mosquitoes were identified using morphological keys and species-specific polymerase chain reaction (PCR) assays. Susceptibility tests were conducted on 2- to 5-day-old non-blood-fed adult female Anopheles that emerged using insecticide-impregnated papers with 0.1% bendiocarb and 0.25% pirimiphos-methyl following standard World Health Organization (WHO) insecticide susceptibility bioassays. …”
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Genetic Polymorphisms of Interleukin-1 Alpha and the Vitamin D Receptor in Mexican Mestizo Patients with Intervertebral Disc Degeneration by Salvador Cervin Serrano, Dalia González Villareal, Maribel Aguilar-Medina, Jose Guillermo Romero-Navarro, Jose Geovanni Romero Quintana, Eliakym Arámbula Meraz, Ignacio Osuna Ramírez, Veronica Picos-Cárdenas, Julio Granados, Iris Estrada-García, Guzman Sánchez-Schmitz, Rosalío Ramos-Payán

“…Gene polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by age and gender: patients with symptomatic lumbar IDD n=100 and subjects with normal lumbar-spine MRI-scans n=100. …”
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Association of tumour mutation burden with prognosis and its clinical significance in stage III gastric cancer by Ya-Lin Han, Li Chen, Xu-Ning Wang, Mao-Lin Xu, Rui Qin, Fang-Ming Gong, Peng Sun, Hong-Yi Liu, Zhi-Peng Teng, Zhao-Xia Li, Guang-Hai Dai

“…Western blot and quantitative real-time polymerase chain reaction were used to detect the expression levels of both proteins and genes. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). …”
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Genomic analysis of the SMN1 gene region in patients with clinically diagnosed spinal muscular atrophy: a retrospective observational study by Tamaki Kato, Mamoru Yokomura, Yutaka Osawa, Kensuke Matsuo, Yuji Kubo, Taihei Homma, Kayoko Saito

“…SNV analysis of SMN1 (exon 1–8 and intron 1–7) was conducted by long-range polymerase chain reaction and next-generation sequencing. Results From 336 DNA samples collected from patients, 62 patient samples were included in the SNV analysis. …”
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Haptoglobin Gene Polymorphism among Sickle Cell Patients in West Cameroon: Hematological and Clinical Implications by Christian Bernard Kengne Fotsing, Constant Anatole Pieme, Prosper Cabral Biapa Nya, Jean Paul Chedjou, Samuel Ashusong, Gisele Njindam, Jocelyn Tony Nengom, Georges Teto, Carine Nguemeni, Wilfred Fon Mbacham, Donatien Gatsing

“…Haptoglobin genotype of 102 SCPs (SS) and 115 healthy individuals (60 AA and 55 AS) was determined by allele-specific polymerase chain reaction, and the complete blood count was determined using the AutoAnalyser. …”
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Salivary peptidomic profiling of chronic gingivostomatitis in cats by matrix‐assisted laser desorption/ionization‐time‐of‐flight mass spectrometry and nanoscale liquid chromatograp... by Sekkarin Ploypetch, Apisit Pornthummawat, Sittiruk Roytrakul, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sabrina Wahyu Wardhani, Sitthichok Lacharoje, Somporn Techangamsuwan

“…Saliva samples were analyzed for viral infections using polymerase chain reaction (PCR), peptide mass fingerprint (PMF) using MALDI‐TOF MS, and peptide identification using nano LC‐MS/MS. …”
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Wastewater Monitoring During the COVID-19 Pandemic in the Veneto Region, Italy: Longitudinal Observational Study by Honoria Ocagli, Marco Zambito, Filippo Da Re, Vanessa Groppi, Marco Zampini, Alessia Terrini, Franco Rigoli, Irene Amoruso, Tatjana Baldovin, Vincenzo Baldo, Francesca Russo, Dario Gregori

“…Wastewater samples were collected weekly from 10 wastewater treatment plants and analyzed using a reverse transcription–quantitative polymerase chain reaction. The CUSUM method was used to detect significant shifts in the data, with an initial analysis conducted from October 2021 to February 2022, followed by validation in a second period from February 2022 to August 2022. …”
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Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25 by Yuanyuan Ying, Fei Wu, Chongyang Wu, Yi Jiang, Min Yin, Wangxiao Zhou, Xinyi Zhu, Cong Cheng, Licheng Zhu, Kewei Li, Junwan Lu, Teng Xu, Qiyu Bao

“…The agar dilution method was conducted to determine the minimum inhibitory concentration (MIC) for florfenicol, and polymerase chain reaction (PCR) was performed to detect florfenicol resistance genes. …”
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Urinary exosomal miRNA-451a can be used as a potential noninvasive biomarker for diagnosis, reflecting tubulointerstitial damage and therapeutic response in IgA nephropathy by Qiong Zhang, Yan Zhao, Yankun Luo, Songjia Guo, Haizhu Hou, Xiaoli Han, Yun Zhou

“…The expression of miR-451a within exosomes was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). uE miR-451a was significantly upregulated in patients with IgAN compared to non-IgAN and HCs. …”
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Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell–Cell Fusion and Immune Activation by Yao Wang, Xunlei Pang, Ruirui Li, Jiuzhou Chen, Chen Wen, Huihuang Zhu, Tingyu Long, Jianjie Li, Lijun Zheng, Youcai Deng, Junnian Zheng, Bo Xu

“…RNA sequencing, quantitative reverse transcription polymerase chain reaction, and Western blot were performed to elucidate the mechanism of syncytia death. …”
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High-Grade Cervical Intraepithelial Neoplasia: Impact of Colposcopic Lesion Area on Systemic Immune Responses by Mantoani PTS, Micheli DC, Jammal MP, Vieira JH, Michelin MA, Ferreira CGX, Silva HN, Murta EFC, Nomelini RS

“…Genotyping for HPV (human papillomavirus) 16, 18, 45 and 52 was performed by PCR (Polymerase Chain Reaction). ROC (Receiver Operating Characteristic) curves were calculated to determine whether there existed a cut-off value for serum cytokines in patients with colposcopic lesion area smaller vs larger than 1 cm2. …”
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Clinical and anamnestic characteristics of patients with clinical manifestations of peripheral artery disease as a function of Т(–786)С polymorphism of the endothelial NO-synthase... by V.I. Tseluyko, L.M. Yakovleva, O.D. Yarova

“…The study of allelic polymorphism of eNOs gene promoter was performed by polymerase chain reaction. Results. According to the results of genotyping of Т(–786)С gene polymorphism of eNOs the share of T/T genotypes in the control group (45.7 %) and in patients with PAD (33 %) was not different (р = 0.18), and C/T more frequently found in the control group (48.6 % vs 26 %; p = 0.01), whereas the prevalence of the C/C genotype in patients with PAD was significantly higher than in healthy individuals (41 % vs 5.7 %; p = 0.0001). …”
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A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran by Sahar Samsami, Sahar Namavari, Saeed Ataei, Abdolmajid Ghasemian, Ava Yazdanpanah, Neda Sepahi, Gholamreza Hatam, Hossein Faramarzi, Hadi Mirzaei, Razie Ranjbar, Ali Ghanbariasad

“…The diagnostic performance of the multiplex LAMP method for VL was compared to each LAMP assay, real-time polymerase chain reaction (RT-qPCR), and nested PCR technique. …”
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Association of serum and local GRP78 and CHOP expressions with disease progression in patients with non-traumatic osteonecrosis of femoral head by Peng Zhang, Qing-He Ye, Wen-Xiu Zhu, Yong-Heng Zhao, Hong-Xun Zhu, Biao-Fang Wei

“…Local CHOP and GRP78 expressions were detected by immunohistochemistry, western blot, alongside real-time polymerase chain reaction (RT-PCR). Radiographic severity was assessed by FICAT grading system. …”
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Interaction of genetics risk score and fatty acids quality indices on healthy and unhealthy obesity phenotype by Niloufar Rasaei, Seyedeh Fatemeh Fatemi, Fatemeh Gholami, Mahsa Samadi, Mohammad Keshavarz Mohammadian, Elnaz Daneshzad, Khadijeh Mirzaei

“…Three single nucleotide polymorphisms-MC4R (rs17782313), CAV-1 (rs3807992), and Cry-1(rs2287161) were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and were combined to produce the genetic risk score (GRS). …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. …”
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Single-cell analyses of intestinal epithelium reveal the dysregulation of gut immune microenvironment in systemic lupus erythematosus by Qiaolin Wang, Yutong Wu, Lianlian Ouyang, Xiaoli Min, Meiling Zheng, Lingyu Gao, Xiaoyun Chen, Zhi Hu, Shuang Yang, Wenjuan Jiang, Sujie Jia, Qianjin Lu, Ming Zhao

“…The primary findings from scRNA-seq were further validated by quantitative polymerase chain reaction (qPCR), flow cytometry, and in vivo experiments including selenium supplementation. …”
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Human Umbilical Cord-Derived Mesenchymal Stem Cell Therapy Ameliorates Nonalcoholic Fatty Liver Disease in Obese Type 2 Diabetic Mice by Bing Li, Yu Cheng, Songyan Yu, Li Zang, Yaqi Yin, Jiejie Liu, Lin Zhang, Yiming Mu

“…Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), immunofluorescence staining, and western blot were performed to monitor the expression of the lipid metabolism- and regulatory pathway-related genes. …”
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