Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used by Anja Šterbenc, Maja M. Lunar, Matjaž Homan, Boštjan Luzar, Nina Zidar, Mario Poljak

“…Various polymerase chain reaction- (PCR-) based methods with varying positivity rates were designed to detect the Helicobacter pylori babA2 gene. …”
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Prognostic values of intracellular cell-related genes in esophageal cancer and their regulatory mechanisms by Wei Cao, Dacheng Jin, Weirun Min, Haochi Li, Rong Wang, Jinlong Zhang, Yunjiu Gou

“…Established methodologies included morphological mapping, enrichment analysis, immune infiltration analysis, prognostic gene expression validation, molecular docking, and Reverse Transcription Polymerase Chain Reaction (RT-PCR) validation. Thirty-eight intersecting genes were identified between the disease and normal groups in ESCA samples. …”
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Association of leptin receptor Gln223Arg genetic polymorphism with obesity and non-alcoholic fatty liver disease by A. V. Morozova, N. V. Mal'tseva, Y. A. Gorbatovsky, O. F. Lykova, S. V. Arkhipova

“…Genotyping for LEPR Gln223Arg gene polymorphism was carried out by allele — specific polymerase chain reaction.Results. In control group and ALD patients no correlation of obesity with LEPR Gln223Arg gene polymorphism was revealed. …”
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Synergistic attenuation of complete freund’s adjuvant-induced inflammation in mice using shinbaro-pelubiprofen: a novel therapeutic complex by Hyunseong Kim, Jin Young Hong, Wan-Jin Jeon, Hyun Kim, Changhwan Yeo, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha

“…Viability and cytotoxicity assays, immunohistochemistry, enzyme-linked immunosorbent assays, real-time polymerase chain reaction assays, nociception assays, writhing tests, and blood biochemical analyses were performed. …”
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Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice by Yingqun Zhou, Weiqi Dai, Chunlei Lin, Fan Wang, Lei He, Miao Shen, Ping Chen, Chenfen Wang, Jie Lu, Ling Xu, Xuanfu Xu, Chuanyong Guo

“…Levels of inflammatory cytokines with ConA-induced hepatitis were determined with real-time polymerase chain reaction (real-time PCR). The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining. …”
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Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation by Ji Zhang, Xuheng Jiang, Chao Zhang, Jun Zhong, Xuanyu Fang, Huanhuan Li, Fangke Xie, Xiaofei Huang, Xiaojun Zhang, Quan Hu, Hongfei Ge, Anyong Yu

“…Here, primary NSCs were cultured and the expression of actin alpha 2 (ACTA2) in NSCs was determined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining. Next, the role of ACTA2 in regulating NSC migration and the potential mechanism was explored. …”
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The expression and clinical significance of serum exosomal-long non-coding RNA DLEU1 in patients with cervical cancer by Yu Chen, Facai Cui, Xiaoyu Wu, Weifeng Zhao, Qingxin Xia

“…However, the potential clinical significance of DLEU1 in serum exosomes of patients with CC remains unclear.Methods The expression of serum exosomal DLEU1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR). A receiver operating characteristic (ROC) curve was plotted to evaluate the clinical diagnostic efficacy of DLEU1. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…Methods Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. …”
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A Systematic Review of the Prevalence and Diagnostic Workup of PIK3CA Mutations in HR+/HER2– Metastatic Breast Cancer by Elizabeth J. Anderson, Lea E. Mollon, Joni L. Dean, Terri L. Warholak, Ayal Aizer, Emma A. Platt, Derek H. Tang, Lisa E. Davis

“…The median prevalence of PIK3CA mutation was 36% (range: 13.3% to 61.5%); identified testing approaches more commonly used tissue over liquid biopsies and primarily utilized next-generation sequencing (NGS), polymerase chain reaction (PCR), or Sanger sequencing. There was concordance and stability between tissues (range: 70.4% to 94%) based on limited data. …”
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MATN3 Gene Polymorphism Is Associated with Osteoarthritis in Chinese Han Population: A Community-Based Case-Control Study by Jiaao Gu, Jiesheng Rong, Fulin Guan, Liying Jiang, Shuqing Tao, Guofa Guan, Tianzun Tao

“…After obtaining the DNA of case and control groups, genotypes of the MATN3 SNP6 were determined by polymerase chain reaction followed by restriction enzyme digestion. …”
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Effects of coal mine dust on lung function in rats by LIU Yang, LI Meng, LU Liyuan, WANG Ru, YANG He, ZHANG Huifang

“…The relative mRNA expressions of peroxisome proliferators-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (TFAM) were detected using real-time fluorescent quantitative polymerase chain reaction assay. The relative protein expressions of PGC-1α and TFAM were detected using Western blotting. …”
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Protective Effect of High-Intensity Interval Training (HIIT) and Moderate-Intensity Continuous Training (MICT) against Vascular Dysfunction in Hyperglycemic Rats by Nurul Paramita, Brilliant C. Puspasari, Randika Arrody, Neng T. Kartinah, Trinovita Andraini, Julfiana Mardatillah, Hardiyanti Rusli, Dewi I. S. Santoso

“…Quantitative real-time polymerase chain reaction (PCR) was used to measure the expression of the receptor for advanced glycation end-products (RAGE) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). …”
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DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression by Rui Hua, Qingjun Chu, Feiyan Guo, Qinjie Chen, Maocai Li, Xuan Zhou, Yongtong Zhu

“…Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real-time polymerase chain reaction. Mouse-derived GC-1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. …”
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Prognostic importance of human leukocyte antigen DRβ1 gene and protein tyrosine phosphatase nonreceptor Type 22 gene polymorphism in rheumatoid arthritis by Pramod Kumar Verma, Usha Singh, Shyam Kumar Saraf, Narendra Kumar Singh

“…HLA DRβ1 typing was done by low-resolution sequence-specific primer polymerase chain reaction and auto-antibodies (Abs) (rheumatoid factor [RF], anti-cyclic citrullinated peptide 2 (CCP2) Ab, anti-nuclear Ab, double-stranded deoxyribose nucleic acid, anticardiolipin Ab) were done by enzyme-linked immunosorbent assay. …”
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Changes in Methylation Patterns of Tumor Suppressor Genes during Extended Human Embryonic Stem Cell Cultures by Kyung Min Kang, Jeoung Eun Lee, Ji Eun Park, Hyunjin Kim, Hee Yeon Jang, Minyeon Go, Dong Ryul Lee, Sung Han Shim

“…CGI methylation levels of 24 tumor suppressor genes were analyzed using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA), pyrosequencing, and real-time polymerase chain reaction (PCR). Different CGI methylation patterns of CASP8, FHIT, and CHFR genes were identified in between early and late passages in some hESC lines. …”
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Investigation of malaria vectors Anopheles in non-endemic areas of Thailand: in proximity to workplaces housing foreign migrant workers by Nurhadi Eko Firmansyah, Thaksaporn Thongseesuksai, Thidarut Boonmars, Porntip Laummaunwai

“…Subsequently, the collected female mosquitoes underwent morphological identification using Standard Thailand keys and polymerase chain reaction analysis with rDNA ITS2 primers. …”
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Evaluation of the Urine POC-CCA Test Accuracy in the Detection of Schistosoma mansoni Infection: A Systematic Review and Meta-Analysis by Getaneh Alemu, Endalkachew Nibret

“…Single POC-CCA test resulted in a pooled sensitivity estimate of 0.81 (95% CI: 0.78–0.84) as evaluated by the polymerase chain reaction (PCR) reference test. Conclusions. …”
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Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages by Ivana Beatrice Mânica da Cruz, Cibele Ferreira Teixeira, Neida Luiza Pellenz, Moisés Henrique Mastella, Verônica Farina Azzolin, Euler Esteves Ribeiro, Fernanda Barbisan

“…The effects on cell proliferation rate and the concentration and production of transcripts of pro-inflammatory cytokines interleukin 1β (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), as well as the anti-inflammatory cytokine IL-10, were assessed using spectrophotometric, immunoassay, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) techniques. Results: S. adstringens extract at all concentrations tested here reduced the cellular proliferation rate of activated macrophages. …”
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Association Between GSK3β Gene Polymorphisms (rs334558 and rs3755557) with Schizophrenia Risk by Maryam Montazeri Dehbarez, Dormohammad Kordi Tamandani, Nasim Naeimi, Shahram Vaziri, Mohammad Shekari

“…Then, we used a polymerase chain reaction (PCR) followed by the restriction fragment length polymorphism method (PCR-RFLP) to examine the GSK3ß SNPs (rs334558 and rs3755557) patterns. …”
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Genotyping <em> Escherichia coli </em> O157:H7 strains isolated from cattle and small ruminants in Fars province (Iran) using Enterobacterial Repetitive Intergenic Consensus PCR A... by Sedigheh Mokhtari, Yahya Tahamtan, Mohammad Kargar, Keyvan Tadayon, Elham Moazamian

“…Methods: An Enterobacterial Repetitive Intergenic Consensus (ERIC) polymerase chain reaction (PCR) was used to determine the genetic relationships and potential cross-contamination among isolates. …”
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