Effect of glyphosate on renal function: A study integrating epidemiological and experimental evidence by Lin Hu, Mingcong Chen, Xiaoran Xue, Mingyi Zhao, Qingnan He

“…The underlying mechanism of the association between glyphosate and renal function was explored by the real-time quantitative polymerase chain reaction (RT-qPCR) and non-targeted metabolomics analysis [embryos (0.75 hpf–96 hpf) were exposed to 90 μg/mL glyphosate]. 1170 participants were enrolled in the NHANES study. …”
Get full text

No microorganism was detected in amniotic fluid of healthy pregnancies from the second trimester to the delivery by Yu Liu, Jingmei Ma, Xiang Li, Haijuan Zhao, Qubo Ai, Lanying Zhang, Yulong Tong, Lingzhen Meng, Huixia Yang

“…Microbial profiles were assessed through cultivation, quantitative real-time polymerase chain reaction, 16S ribosomal RNA gene sequencing, and cytokine analysis. …”
Get full text

Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital by Funda Şahin, Nida Özcan, Erdal Özbek, Selahattin Atmaca, Hakan Temiz

“…The aim of our study was to retrospectively determine the causative agents in cerebrospinal fluid samples of patients who received a presumptive diagnosis of meningitis, using Multiplex Polymerase Chain Reaction (PCR) tests. Materials and Methods: The study included 206 cerebrospinal fluid samples from different patients with a preliminary diagnosis of meningitis sent from various clinics. …”
Get full text

Lactate Ameliorates Kainic Acid-Induced Neuroinflammation and Cognitive Impairment via the Chemokine Signaling Pathway in Mice by Chu X, Ge Y, Geng C, Cao P, Wei P, Fu B, Deng Z, Li Y, Zhao G

“…The neuronal damage, microglial activation, inflammatory responses, and functional changes were determined by TUNEL assays, immunohistochemistry, quantitative real-time polymerase chain reaction and behavioral tests. The differentially gene expression and functional enrichment were analyzed with RNA sequencing.Results: The in vitro lactate intervention reduced the number of apoptotic cells, the release of inflammatory factors, and the expression of vesicular glutamate transporter 1. …”
Get full text

Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain by Gui-Lin Jin, Sai-Di He, Shao-Mei Lin, Li-Mian Hong, Wan-Qing Chen, Ying Xu, Jian Yang, Su-Ping Li, Chang-Xi Yu

“…Western blot analysis and quantitative real-time polymerase chain reaction (qPCR) were used to examine the modulator effect of koumine on microglial M1 polarization. …”
Get full text

Xiaoyao Pills Attenuate Inflammation and Nerve Injury Induced by Lipopolysaccharide in Hippocampal Neurons In Vitro by Yang Fang, Boyu Shi, Xiaobo Liu, Jie Luo, Zhili Rao, Rong Liu, Nan Zeng

“…The expression levels of IL-6, TNF-α, IDO, 5-HT, brain-derived neurotrophic factor, and β-nerve growth factor were detected by enzyme-linked immunosorbent assay. mRNA levels of IL-6, TNF-α, 5-HT1A, IDO-1, brain-derived neurotrophic factor, nerve growth factor, tropomyosin receptor kinase B, tropomyosin receptor kinase A, and cAMP response element-binding protein were detected by reverse transcription-polymerase chain reaction. To further validate, protein expression was determined by western blot and immunofluorescence. …”
Get full text

Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens by Amira M. Helal, Osama Mahana, Ali Zanaty, Karim Selim, Mohamed. E. Taha, Mostafa M. Saleh, Mohamed I. Hassan, Mona S. Abdallah

“…Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. …”
Get full text

Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells by Yijun Yao, Zhifang Li, Bowen Qin, Xingrong Ju, Lifeng Wang

“…We also preliminary studied the lipid-lowering mechanism by Real-time fluorescent quantitative polymerase chain reaction (qPCR). The results indicated that highland barley extract contains many components with lipid-lowering effects, such as hyperoside and scoparone. …”
Get full text

RNA-Sequence Reveals the Regulatory Mechanism of miR-149 on Osteoblast Skeleton under Mechanical Tension by Yifan Wang, Guanyin Zhu, Fang Pei, Yigan Wang, Jun Liu, Caixia Lu, Zhihe Zhao

“…Real-time quantitative polymerase chain reaction (RT-qPCR) detected the expression of miR-149 and ARFGAP with SH3 domain, Ankyrin repeat, and Ph domain 3 (ASAP3) mRNA. …”
Get full text

Downregulation of TNF-α/TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury by Suhui Yu, Jianming Xie, Yukai Xiang, Shengjie Dai, Dinglai Yu, Hongwei Sun, Bicheng Chen, Mengtao Zhou

“…We used western blot, polymerase chain reaction (PCR), and immunofluorescence to investigate the downregulation of TNF-α signals in cellular and animal models of SAP-associated lung injury following AT-Lipoxin A4 intervention. …”
Get full text

Occupationally Acquired American Cutaneous Leishmaniasis by Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Éricka Lima de Almeida, Ângela Cristina Rapela Medeiros, Roberto Pereira Werkhäuser, Ana Isabele Freitas de Araújo, Sinval Pinto Brandão-Filho, Alzira Maria Paiva de Almeida, Eduardo Henrique Gomes Rodrigues

“…We report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for Leishmania, subgenus Viannia. …”
Get full text

lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis by Shan-zheng Wang, Jun Jia, Chang-hong Chen

“…The expression of miRNA-141-5p (miR-141-5p) and lnc-KCNQ1OT1 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression of cleaved-caspase-3, caspase-3, and Bax was determined by Western blot. …”
Get full text

Utility of Ascitic Fluid Adenosine Deaminase Levels in the Diagnosis of Tuberculous Peritonitis in General Medical Practice by Ayako Kumabe, Shuji Hatakeyama, Naoki Kanda, Yu Yamamoto, Masami Matsumura

“…Tuberculous peritonitis is difficult to diagnose due to its varying clinical features, in addition to the low yield on bacterial culture or polymerase chain reaction using ascitic fluid samples. This study aimed to investigate the sensitivity and specificity of elevated adenosine deaminase (ADA) levels as a diagnostic marker for tuberculous peritonitis. …”
Get full text

TSP-1-1223 A/G Polymorphism as a Potential Predictor of the Recurrence Risk of Bladder Cancer in a Chinese Population by Xiao Yang, Pengchao Li, Xuejian Yang, Chao Qin, Qiang Cao, Zhengdong Zhang, Meilin Wang, Hongzhou Cai, Jinbao Gu, Jun Tao, Min Gu, Qiang Lu, Changjun Yin

“…Quantitative real-time polymerase chain reaction was performed to determine TSP-1 mRNA expression. …”
Get full text

Watermelon Germplasm with Resistance to Whitefly-transmitted Viruses by Alex Luckew, Nebahat Sari, Swikriti Pandey, Ted McAvoy, Alvin M. Simmons, Geoffrey Meru, Cecilia McGregor

“…Leaves were collected 5 weeks after transplanting (WAT) in 2022 and at 7 WAT in 2023 to determine the viral loads of Cucurbit leaf crumple virus (CuLCrV), Cucurbit yellow stunting disorder virus (CYSDV), and Cucurbit chlorotic yellows virus (CCYV) via a quantitative polymerase chain reaction. The CuLCrV and CYSDV viral loads were higher in 2022 than those in 2023, whereas the CCYV load was higher in 2023. …”
Get full text

Prevalence of ESBL resistance genes and fecal carriage of multidrug-resistant Pseudomonas aeruginosa isolates from patients with chronic kidney disease at the Laquintinie Hospital... by Ballue S.T. Dadjo, Armelle T. Mbaveng, Michael F. Kengne, Ornella D. Tsobeng, Giresse D.T. Fonjou, Victor Kuete

“…Antibiotic susceptibility testing was carried out using the Kirby-Bauer diffusion method. extended-spectrum β-lactamase (ESBL) resistance genes were detected by the polymerase chain reaction (PCR) method. Results: The prevalence of fecal carriage of Pseudomonas aeruginosa was 9.17 % (n = 42/458), including twenty-nine (69.05 %) in patients with chronic kidney disease and thirteen (30.95 %) in patients without chronic kidney disease. …”
Get full text

Comparison of Chest Computed Tomography (CT) Imaging Patterns and Severity among COVID-19 Patients during the First and Fourth Waves in Addis Ababa, Ethiopia by Lensa Million Baharu, Amir Alwan, Seife Teferi Dellie, Tesfaye Kebede Legesse, Kibruyisfaw Weldeab Abore

“…An institution-based cross-sectional study was conducted on 200 polymerase chain reaction test confirmed COVID-19 patients who underwent chest CT scan imaging in two diagnostic centers in Addis Ababa city. …”
Get full text

Virological and Immunological Aspects of AIDS Pathogenesis by Brian Conway, Francisco J Diaz-Mitoma

“…In the authors' laboratories, a quantitative polymerase chain reaction assay for the evaluation of circulating proviral load has been developed. …”
Get full text

Grazing mortality as a controlling factor in the uncultured non-cyanobacterial diazotroph (Gamma A) around the Kuroshio region by T. Sato, T. Sato, T. Yamaguchi, K. Hidataka, S. Sogawa, T. Setou, T. Kodama, T. Kodama, T. Shiozaki, K. Takahashi

“…In this study, we investigated the diazotroph community structure using <i>nifH</i> amplicon sequencing and quantified the growth and microzooplankton grazing mortality rate of Gamma A using a combination of dilution experiments and quantitative polymerase chain reaction (PCR) in well-lit waters at the northern edge of the Kuroshio Current off the southern coast of Japan. …”
Get full text

Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients by Maria Luisa Manca Bitti, Speranza Masala, Francesca Capasso, Novella Rapini, Simona Piccinini, Federica Angelini, Andrea Pierantozzi, Roberta Lidano, Silvia Pietrosanti, Daniela Paccagnini, Leonardo A. Sechi

“…MAP DNA was detected using IS900-specific polymerase chain reaction (PCR). The presence of antibodies towards a MAP antigen, heparin binding hemoagglutinin (HBHA), was detected by ELISA. …”
Get full text