lnc-MRGPRF-6:1 Promotes ox-LDL-Induced Macrophage Ferroptosis via Suppressing GPX4 by Zhihuan You, Xiaotian Ye, Meihua Jiang, Ning Gu, Caihong Liang

“…Then, transcriptome sequencing, literature searching, and following quantitative real-time polymerase chain reaction and western blot were implemented to explore specific signaling pathway in the process. …”
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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8 by Zhongsi Hong, Haiyu Hong, Jian Liu, Xiaobin Zheng, Mingxing Huang, Chunna Li, Jinyu Xia

“…Materials and Methods. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. …”
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Development of RT-PCR Assays for Simple Detection and Identification of Sabin Virus Contaminants in the Novel Oral Poliovirus Vaccines by Olga Singh, Hasmik Manukyan, Erman Tritama, Shwu-Maan Lee, Jerry P. Weir, Majid Laassri

“…Methods: This study describes the development of pan quantitative reverse transcription polymerase chain reaction (panRT-PCR) and multiplex one-step RT-PCR (mosRT-PCR) assays for the straightforward detection and identification of contaminating Sabin viruses when present in significantly higher amounts of nOPV strains. …”
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Multi-Host of Ectomycorrhizal Fungi on Dipterocarpaceae in Tropical Rain Forests by Maliyana Ulfa, Eny Faridah, Su See Lee, Sumardi Sumardi, Christine le Roux, Antoine Galiana, Patahayah Mansor, Marc Ducousso

“…The research was conducted by identifying the ectomycorrhizal fungi via molecular approach by using Polymerase Chain Reaction (PCR) technique. To strengthen the sequence of DNA extracts, a specific primer pair of ITS 1F-ITS 4 was used. …”
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Correlation of Clinical Severity and Laboratory Parameters with Various Serotypes in Dengue Virus: A Hospital-Based Study by Pooja Rao, Achappa Basavaprabhu, Suchitra Shenoy, Nikhil Victor Dsouza, Basavaiah Sridevi Hanaganahalli, Vaman Kulkarni

“…Detection of dengue serotypes was done by multiplex reverse transcriptase polymerase chain reaction (RT_PCR). Results. Out of 106 RT-PCR-confirmed cases, DENV-3 was the most common serotype found in 56 (52.8%) patients, followed by DENV-3 and DENV-4 coinfection in 27 (25.4%) patients. …”
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Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolated in Canadian Hospitals: Results from CANWARD 2007 by Patricia J Baudry, Melissa McCracken, Philippe Lagacé-Wiens, Michael R Mulvey, George G Zhanel, Daryl J Hoban

“…Minimum inhibitory concentrations were determined by broth microdilution, and putative ESBL isolates were confirmed by the Clinical and Laboratory Standards Institute disk diffusion method. Polymerase chain reaction and DNA sequencing were used to detect blaSHV, blaTEM, blaCTX-M and blaOXA-like genes. …”
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Combination Therapy with Platelet-Rich Plasma and Epidermal Neural Crest Stem Cells Increases Treatment Efficacy in Vascular Dementia by Somayeh Akbari, Masoud Haghani, Mojtaba Ghobadi, Etrat Hooshmandi, Afshin Borhani Haghighi, Mohammad Saied Salehi, Sareh Pandamooz, Negar Azarpira, Afsoon Afshari, Shahrbanoo Zabihi, Marzieh Nemati, Mahnaz Bayat

“…The mRNA expression levels of IGF-1, TGF-β1, PSD-95, and GSk-3β were measured in the rat hippocampus by quantitative reverse transcription polymerase chain reaction. Results. The results demonstrated impaired learning, memory, and synaptic plasticity in the 2VO rats, along with a significant decrease in the expression of IGF-1, TGF-β1, PSD-95, and upregulation of GSK-3β. …”
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High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas by Shiwani Singh, Maneesh S. Bhandari, Neeraj Dwivedi

“…Also, the isolation of DNA in purified form has its significance in facilitating precise genetic analysis such as sequencing, restriction digestion and polymerase chain reaction (PCR)-based trials. Setting: This study was conducted under controlled laboratory environment at Manav Rachna Centre for Medicinal Plant Pathology, Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, India. …”
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Mortality Prediction in the Emergency Service Intensive Care Patients with Possible COVID-19: A Retrospective Cross-Sectional Study by Mehmed Ulu, M. Kaya, Y. Tunc, H. Yildirim, A. Halici, A. Coskun

“…RESULTS: Of the 397 patients, 111 (28 %) patients had positive polymerase chain reaction (PCR). 75 (67.6 %) patients deceased in the PCR positive group while 163 (57.0 %) patients deceased in the negative group (p > 0,05). …”
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Massive Pulmonary Embolism Complicating Coronavirus Disease 2019 (COVID-19) Pneumonia: A Case Report by Shruti Hegde, Gemini Yesodharan, John Tedrow, Alena Goldman

“…His swab turned positive for SARS-CoV-2 infection on real-time reverse transcriptase-polymerase chain reaction assay (RT-PCR) on day 2 of his ED visit. …”
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Molecular Diagnosis of Periprosthetic Joint Infection by Quantitative RT-PCR of Bacterial 16S Ribosomal RNA by Mel S. Lee, Wen-Hsin Chang, Su-Chin Chen, Pang-Hsin Hsieh, Hsin-Nung Shih, Steve W. N. Ueng, Gwo-Bin Lee

“…This study used quantitative reverse transcription polymerase chain reaction (RT-qPCR) to amplify bacterial 16S ribosomal RNA in vitro and in 11 clinical samples. …”
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Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia by Abdulaziz Alqasim, Ahmad Abu Jaffal, Abdullah A. Alyousef

“…ESBL production was characterized at phenotypic and genotypic levels using double-disc synergy test and polymerase chain reaction, respectively. Detection of different ESBL variants was performed using DNA sequencing. …”
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Latent Toxoplasmosis among Breast Cancer Patients in Jahrom, South of Iran by Marzeieh Haghbin, Salar Maani, Mohammad Aref Bagherzadeh, Ahmadreza Bazmjoo, Heshmatollah Shakeri, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Amir Abdoli

“…Molecular detection was performed by polymerase chain reaction (PCR) using the primer pair targeting the repetitive element (RE) gene of T. gondii. …”
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Knockdown of YAP/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating Autophagy by Wei Zhou, Qin Shen, Hui Wang, Jie Yang, Chen Zhang, Zijing Deng, Keyan Wu, Yang Zhou, Jing Zeng, Yu Zhang, Weigan Shen

“…Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting assays were performed to examine the expression of indicated genes. …”
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Patterns of Antibiotic Resistance in Enterobacteriaceae Isolates from Broiler Chicken in the West Region of Cameroon: A Cross-Sectional Study by Jude Fonbah Leinyuy, Innocent Mbulli Ali, Ousenu Karimo, Christopher B. Tume

“…Plasmid-borne genes for extended-spectrum beta lactamase (ESBL) and resistance to Quinolones (PMQR) and Aminoglycosides were detected by standard endpoint polymerase chain reaction (PCR). Results. A total of 394 isolates were identified belonging to 12 Enterobacteriaceae genera, the most prevalent were Escherichia coli (81/394 = 20.56%), Salmonella spp (74/394 = 18.78%), and Klebsiella spp (39/394 = 9.90%) respectively. …”
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Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia by Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka

“…Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method. 17 out of 359 blood samples (4.7%) were positive for the isolation of DENV. …”
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The Effect of Chromium Picolinate Supplementation on the Pancreas and Macroangiopathy in Type II Diabetes Mellitus Rats by Shan Huang, Wenfang Peng, Xiaohong Jiang, Kan Shao, Lili Xia, Yubin Tang, Jiayin Qiu

“…Real-time reverse transcription polymerase chain reaction (RT-PCR) was applied for detecting the mRNA expression of APN and apelin. …”
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Clinical performance of real-time nanopore metagenomic sequencing for rapid identification of bacterial pathogens in cerebrospinal fluid: a pilot study by Yoon Hyun Sung, Yong Kuk Ju, Hak Jun Lee, Seung Min Park, Jin Woong Suh, Jeong Yeon Kim, Jang Wook Sohn, Young Kyung Yoon

“…We conducted a 16S rRNA amplicon sequencing using a nanopore-based platform, alongside routine polymerase chain reaction (PCR) testing or bacterial culture, to compare its clinical performance in pathogen detection on CSF samples. …”
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Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids by Somyeong Hwa, Hyun-Jin Lee, Youngkyung Ko, Jun-Beom Park

“…Additionally, quantitative polymerase chain reaction (qPCR) analysis was performed to determine the expression of RUNX2 and COL1A1. …”
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AMPK Activation in TET2 Downregulated Leukemia Cells Upon Glutamine Limitation by Ahsen Merve BAYRAK, Burcu YUCEL

“…GLUL mRNA expression was investigated using quantitative reverse transcription polymerase chain reaction and protein levels were evaluated using immunoblotting. …”
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