Evaluation of Anti-Cancer, Pro-Apoptotic, and Anti-Metastatic Effects of Synthetic Analogue Compound 2-Imino-7-Methoxy-4-(4-fluorophenyl)-2H-1,3-Thiazino [3,2-a] Benzimidazole on P... by Mpho Ndou, Marthe C. D. Fotsing, Michael H. K. Kengne, Edwin M. Mmutlane, Derek T. Ndinteh, Mthokozisi B.C. Simelane, Lesetja Motadi, Mpho Choene

“…Gene expression was analyzed using conventional polymerase chain reaction (PCR). Cell migration was assessed using wound healing. …”
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circRNA Expression Profile in Dental Pulp Stem Cells during Odontogenic Differentiation by Ming Chen, Yeqing Yang, Junkai Zeng, Zilong Deng, Buling Wu

“…Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was then performed to validate the microarray results. …”
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Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer by Naomi Yoshuantari, Didik Setyo Heriyanto, Susanna Hilda Hutajulu, Johan Kurnianda, Ahmad Ghozali

“…The quantitative expressions of CXCL12 and CXCR4 messenger RNA (mRNA) were evaluated in 32 patients with adenocarcinoma-type CRC. Real-time polymerase chain reaction (qRT-PCR) was performed on formalin-fixed tissues. …”
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Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model by Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji

“…Minor sequence polymorphisms, 1.67%, are observed in one of the isolates, whereas remarkable DNA sequence polymorphisms are noticed in three of the isolates. The polymerase chain reaction (PCR) products sequencing profiles revealed 100% polymorphisms in four of the isolates in comparison with the source gene (AY569356.1), instead, those isolates reveal various degrees of analogy, 80.33%, 80.87–89.05%, and 89.36% to G1, G3, and G6, respectively. …”
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The role of SASP in ischemic stroke: a deep dive into cellular mechanisms by Dong Xie, Yang Liu, Fang-Biao Xu, Jin-Sheng Zhang

“…Finally, authenticated core gene expression in serum of MCAO and Sham rats using real-time fluorescent polymerase chain reaction (RT-qPCR).ResultsFourteen hub genes were discerned. …”
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CDC25B Is a Prognostic Biomarker Associated With Immune Infiltration and Drug Sensitivity in Hepatocellular Carcinoma by Zixiang Huang, Liangzhi Xu, Zhengqiang Wu, Xiaofeng Xiong, Linfei Luo, Zhili Wen

“…CDC25B expression was verified in clinical HCC tissue samples using fluorescence quantitative polymerase chain reaction (q-PCR) and protein immunoblotting (Western blot). …”
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Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors by Behnaz Esmaeili, Behnaz Bayat, Atefe Alirezaee, Mona Delkhah, Mohammad Reza Mehdizadeh, Zahra Pourpak

“…HNA-1, HNA-3, HNA-4, and HNA-5 were genotyped, using the polymerase chain reaction sequence-specific primer (PCR-SSP) technique. …”
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Association between asymptomatic submicroscopic and microscopic malaria infections and anemia: A study in southern Benin. by Alejandro Rojas Chaves, Yannelle Dossou, Armel Djènontin, Elisée Adimi, Romuald Akoho, Justine Bailly, Aziz Bouraïma, Déborah Matondo, Yolande Sissinto, Dismand Houinato, Achille Massougbodji, Célia Dechavanne, Gilles Cottrell

“…Participants were monitored over two months and assessed for malaria infection through microscopy and polymerase chain reaction (PCR) during their three visits. …”
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Adiponectin Gene Polymorphisms and Possible Susceptibility to Metabolic Syndrome among the Sudanese Population: A Case-Control Study by Altaf S. Mosad, Ghada A. Elfadil, Alsadig Gassoum, Khanssa M. Elamin, Nazik Elmalaika Obaid Seid Ahmed Husain

“…The guanidine extraction procedure was used to extract genomic DNA from EDTA whole blood by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP); samples were genotyped for the polymorphisms (rs266729), (rs2241766), and (rs1501299). …”
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Odontogenic differentiation of dental pulp stem cells by glycogen synthase kinase-3β inhibitory peptides by Vidhyashree Rajasekar, Mohamed Mahmoud Abdalla, Mohammed S. Basbrain, Prasanna Neelakantan, Cynthia KY Yiu

“…The differentiation of hDPSCs following peptide treatment was determined using the alkaline phosphatase assay (ALP), calcium mineralization (alizarin red staining), and quantification of mRNA expression of differentiation markers via quantitative real-time polymerase chain reaction. The accumulation of β-catenin in the nucleus of GSK3-βi-treated hDPSCs was determined using immunofluorescence staining. …”
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Detection of Mycoplasma spp. from snakes from five different families by Marline M. Faulhaber, Florence Tardy, Franziska Saul, Elisabeth Müller, Michael Pees, Rachel E. Marschang

“…To better understand the occurrence of these bacteria in other snake species, samples submitted for routine testing for respiratory pathogens were also tested for mycoplasma by polymerase chain reaction (PCR). A total of 640 samples (mostly oral swabs) from snakes of 5 different families (Boidae n = 114, Colubridae n = 109, Elapidae n = 34, Pythonidae n = 301 and Viperidae n = 82) were included in the study. …”
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Bromhexine Hydrochloride Prophylaxis of COVID-19 for Medical Personnel: A Randomized Open-Label Study by Evgeny N. Mikhaylov, Tamara A. Lyubimtseva, Aleksandr D. Vakhrushev, Dmitry Stepanov, Dmitry S. Lebedev, Elena Yu. Vasilieva, Alexandra O. Konradi, Evgeny V. Shlyakhto

“…The composite primary endpoint was a positive nasopharyngeal swab polymerase chain reaction (PCR) test for SARS-CoV-2 or signs of clinical infection within 28 days and at week 8. …”
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Study on Management of Blood Transfusion Therapy in Patients with Hereditary Spherocytosis by Shiyue Ma, Lingjian Tang, Chaoli Wu, Hui Tang, Xue Pu, Jinhong Niu

“…DNA from all family members of the 3 HS probands was amplified by polymerase chain reaction (PCR) and sequenced by the Sanger method to assess genetic relation for HS. …”
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Positive Pneumocystis jirovecii Sputum PCR Results with Negative Bronchoscopic PCR Results in Suspected Pneumocystis Pneumonia by Kelly Pennington, John Wilson, Andrew H. Limper, Patricio Escalante

“…The diagnostic standard for Pneumocystis jirovecii pneumonia (PCP) is direct microscopic identification; however, in recent years, polymerase chain reaction (PCR) from bronchoalveolar lavage (BAL) samples to detect Pneumocystis nucleic acids has proven to be more sensitive and specific. …”
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Composition and Significance of Bacterial Microbiota and Volatile Organic Compounds of Swiss-Dutch-Type Cheese as Determined by PCR-DGGE and HS-GC by Beata Nalepa, Magdalena Anna Olszewska, Lidia Hanna Markiewicz, Marek Aljewicz

“…Bacterial diversity and VOCs (acetaldehyde; ketones: acetone, diacetyl, acetoin; alcohols: methanol, ethanol; esters: ethyl acetate, ethyl propionate, ethyl butyrate; fatty acids: acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, isocaproic acid, caproic acid, heptanoic acid) were determined by polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE), and headspace gas chromatography (HS-GC), respectively. …”
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Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania by Hyasinta Jaka, Nele Rüttgerodt, Wolfgang Bohne, Andreas Mueller, Uwe Gross, Christa Kasang, Stephen E. Mshana

“…Biopsies were taken for polymerase chain reaction (PCR) and sequencing to detect mutations conferring resistance to clarithromycin and fluoroquinolones. …”
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Inhibitory Effect of Lactobacillus delbrueckii subsp. bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage by Pan Wang, Fang Tan, Jianfei Mu, Hongjiang Chen, Xin Zhao, Yanan Xu

“…Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments. The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice. …”
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MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Quantitative polymerase chain reaction (qPCR) was used to detect the expression of MAC30 in 68 patients with gastric cancer and their adjacent tissues. …”
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m6A-modified lncRNA GAS5 promotes M1-polarization of microglia in alcohol use disorder by Shuang Zhu, Peng Wang, Jian Hu

“…We assessed the expression levels of long noncoding RNA GAS5 (lncRNA GAS5) and microRNA-136–5p (miR-136–5p) in AUD tissue samples and cell lines using reverse transcription-quantitative polymerase chain reaction. Detection of GAS5 N6-methyladenosine (m6A) modifications, facilitated by alkylation repair homolog 5 (ALKBH5), was performed using RNA immunoprecipitation and RNA pull-down assays. …”
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Killer-Cell Immunoglobulin-Like Receptors (KIR) in HIV-Exposed Infants in Cameroon by Kagoué Simeni Luc-Aimé, Yindom Louis-Marie, Loni Ekali Gabriel, Clauvis Kunkeng Yengo, F. Esemu Livo, Nguedia Jules Clement Assob

“…We designed a cross-sectional study to molecularly determine the frequencies of 15 KIR genes in 14 HIV-exposed infected (HEI), 39 HIV-exposed/uninfected (HEU), and 27 HIV-unexposed/uninfected (HUU) infants using the sequence specific primer polymerase chain reaction (PCR-SSP) method. We found that all 15 KIR genes were present in our cohort. …”
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