Gastrointestinal protozoa in pet cats from Anhui province: prevalence and molecular characterization by Hao Zhang, Xing Tong, Zhonghui Ma, Tao Zhang, Feng Wu, Qiong Wu, Congshan Yang, Chunyang Han

“…IntroductionTo investigate the prevalence of Tritrichomonas foetus, Pentatrichomonas hominis, Giardia intestinalis, Cryptosporidium, Microsporidium, and Sarcocystis in domestic cats in Anhui Province, China, and their potential role as zoonotic hosts for human infection, a total of 304 fecal samples from two different sources were screened for the presence of related pathogens.MethodsUsing microscopy, along with polymerase chain reaction (PCR) or nested PCR amplification, followed by genotyping through sequence analysis.ResultsThe infection rates of T. foetus, P. hominis, G. intestinalis, Cryptosporidium, Enterocytozoon bieneusi, and Sarcocystis were 5.6%, 0%, 1.7%, 0.7%, 2.6%, and 0%, respectively. …”
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Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro by Shuai Wu, Zhongliang Cheng, Guohua Liu, Xinfeng Zhao, Liang Zhong, Yingjian Zhu, Jiang Zhu

“…Urothelial cell specific marker uroplakin II (UPII) and cytokeratins were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence technology. …”
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Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assays. by Sukalyani Banik, Kaheerman Saibire, Shraddha Suryavanshi, Glenn Johns, Soumitesh Chakravorty, Robert Kwiatkowski, David Alland, Padmapriya P Banada

“…<h4>Methods</h4>We evaluated a guanidium thiocyanate-based buffer, eNAT™ (Copan) as a possible transport and inactivation medium for downstream Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) testing to detect SARS-CoV-2. …”
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Assessing the influence of matcha green tea extract on NF-κB/MAPK signaling pathway activation in rats: A study on irradiation-induced acute hepatic inflammatory response by Noha Sayed Hamed, Eithar K. El Adham, Amal I. Hassan

“…Liver miR-122 expression levels were determined using real-time reverse transcription polymerase chain reaction. In addition, the present study investigated the mechanism by which M stops the inflammatory process's crucial NF-κB/MAPK signaling. …”
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CpG Oligodeoxynucleotides Inhibit RANKL-Induced Osteoclast Formation by Upregulating A20 Deubiquitinase in RAW 264.7 Cells by Seong-Kyu Kim, Jung-Yoon Choe, Ki-Yeun Park

“…Osteoclast-specific genes and their related signaling molecules were measured by real-time quantitative polymerase chain reaction and Western blot assay. Morphological assessment for osteoclast formation was performed using tartrate-resistant acid phosphatase (TRAP) staining and F-actin ring formation staining. …”
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Two Imported Malaria Cases with Delayed Response to Treatment in Hatay by Tuğba Kaya, Mehmet Çabalak, Gülnaz Çulha, Ceren Ünal

“…The P. falciparum18S rRNA gene was detected using real-time polymerase chain reaction. Artemether-lumefantrine treatment protocol was started. …”
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Aging restricts the initial neural patterning potential of developing neural stem and progenitor cells in the adult brain by Saeideh Aran, Mohammad Ghasem Golmohammadi, Mohsen Sagha, Kamran Ghaedi

“…Thus, the embryonic and adult-derived NSPCs for cardinal characteristics were analyzed, and then, the expression of candidate genes related to neural patterning using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was evaluated at various stages of embryonic (E14 and E18), neonatal, and adult brains. …”
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DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines by E. T. Ilnitskaya, M. V. Makarkina, S. V. Tokmakov, L. G. Naumova

“…The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. …”
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The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats by Caipan Gong, Li Li, Chunmei Qin, Weihua Wu, Qi Liu, Ying Li, Linwang Gan, Santao Ou

“…VSMC markers and osteogenic markers as well as target molecules in Notch1-RBP-Jk/Msx2 were determined by quantitative real-time polymerase chain reaction and immunohistochemical analysis. …”
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Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids by Eliška Zezulová, Tomáš Kiss, Kateřina Baránková, Tomáš Nečas

“…In addition, the genotyping with simple sequence repeat (SSR) markers (PGS1.21, PGS1.23) and ParPMC2 allele-specific polymerase chain reaction (PCR) for the purpose of evaluating PPV resistance. …”
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The Effect of High Levels Carbohydrate on Intestinal Microbiota, Metabolites, and Health of Common Carp (Cyprinus carpio L.) by Jinrui Xiong, Liping Yang, Luming Wang, Shaoyang Zhi, Mengjuan Zhao, Chunchu Xu, Leya Qu, Xiaorui Guo, Xiao Yan, Chaobin Qin, Guoxing Nie

“…Feeding with a HCD led to decreased activity of catalase and increased malondialdehyde content, which was consistent with reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) analysis results (p<0.05). …”
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Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells by Oliver Pieles, Marcus Höring, Sadiyeh Adel, Torsten E. Reichert, Gerhard Liebisch, Christian Morsczeck

“…Expression and activity of metabolic markers were evaluated by western blot analysis and specific assays, relative amount of mitochondrial DNA was measured by real-time quantitative polymerase chain reaction, the oxidative state of cells was determined by a glutathione assay, and the lipidome of cells was analyzed via mass spectrometry (MS). …”
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Incidence, Molecular Detection, and Partial Nucleotide Sequencing of Some Viruses Causing Fig Mosaic Disease (FMD) on Fig Plants in Egypt by Neven I. Toima, Om-Hashem M. El-Banna, Ali M. Sayed, Sahar A. Youssef, Ahmed A. Shalaby

“…Symptomatic samples were tested for the presence of fig mosaic virus (FMV), fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV), fig latent virus 1 (FLV-1), fig fleck-associated virus (FFkaV), and fig cryptic virus (FCV) using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers. Three viruses were detected in mixed infections and showed positive results. …”
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CircTRRAP Knockdown Has Cardioprotective Function in Cardiomyocytes via the Signal Regulation of miR-370-3p/PAWR Axis by Yuan Zhang, Zhenggong Li, Jiao Wang, Hao Chen, Rui He, Hongkun Wu

“…CircTRRAP, microRNA-370-3p (miR-370-3p), and Pro-Apoptotic WT1 Regulator (PAWR) levels were assayed by reverse transcription-quantitative polymerase chain reaction. Cell proliferation analysis was performed via 5-ethynyl-2′-deoxyuridine (EdU) assay. …”
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Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals by El sayed Hamdy Mohammed, Ahmed Elsadek Fakhr, Hanan Mohammed El sayed, Said abd Elmohsen Al Johery, Wesam Abdel Ghani Hassanein

“…Production of blaVIM, blaTEM, blaSHV, and blaCTX-M genes was done by polymerase chain reaction (PCR). Direct sequencing from PCR products was subsequently carried out to identify and confirm these β-lactamases genes. …”
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Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children by Chung-Min Kang, Seong-Oh Kim, Mijeong Jeon, Hyung-Jun Choi, Han-Sung Jung, Jae-Ho Lee

“…The expression of development, chemotaxis, mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSs) related genes was assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Histological analysis was performed using hematoxylin-eosin and immunohistochemical staining. …”
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The Effect of IL-35 on the Expression of Nasal Epithelial-Derived Proinflammatory Cytokines by Mingrong Nie, Qingxiang Zeng, Luo Xi, Yiquan Tang, Renzhong Luo, Wenlong Liu

“…The expression of IL-33, IL-25, TSLP, eotaxin-1, eotaxin-2, and eotaxin-3 from supernatant was measured using real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). …”
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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal by Rúben Fernandes, Paula Amador, Carla Oliveira, Cristina Prudêncio

“…In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. …”
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Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa by Lungile N. Mlangeni, Tsepo Ramatla, Kgaugelo E. Lekota, Cormac Price, Oriel Thekisoe, Che Weldon

“…The isolates were identified using polymerase chain reaction (PCR) by amplifying the invA gene and were further confirmed by 16S rRNA gene sequencing. …”
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Using spectrometric analysis for indirect estimation of 146S component concentration while measuring FMDV RNA amount by M. I. Doronin, D. V. Mikhalishin, N. Ye. Kamalova, A. V. Borisov

“…The actual results obtained in real-time reverse transcription – polymerase chain reaction (rtRT-PCR) were 97.0–99.9% consistent with the expected results of the spectrometric analysis used to determine cultural FMDV 146S component concentration. …”
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