Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran by Farhad Lajmorak, Seyedeh Ommolbanin Ghasemian, Majid Gholami-Ahangaran

“…The conventional microbiological and biochemical tests were applied for isolation and typing of Salmonella spp. The polymerase chain reaction (PCR) was utilized for detection and typing of Salmonella strains. …”
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Integrated Approach for Biomarker Discovery and Mechanistic Insights into the Co-Pathogenesis of Type 2 Diabetes Mellitus and Non-Hodgkin Lymphoma by Zhu Y, Liu J, Wang B

“…Finally, quantitative real-time polymerase chain reaction was performed to validate hub genes in clinical samples.Results: Intersection analysis of DEGs from the T2DM and NHL datasets identified 81 shared genes. …”
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Characterization of patients admitted with pneumonia and a negative diagnostic test for COVID-19 in the intensive care unit by Rodolfo Javier Rivero Morey, Lietter Aguilar Jure, Yenisel Chang Cuesta, Inte Santana Carballosa, Victor René Navarro Machado, Lya del Rosario Magariño Abreus

“…<br /><strong>Methods</strong>: descriptive study, carried out between March/2020 and March/2021, in a series of 338 patients admitted to the Intensive Care Unit with a history of negative polymerase chain reaction to COVID-19, but with a diagnosis of acquired pneumonia in the community. …”
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Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone by Man Yang, Yunqing Pang, Minyu Pei, Yuanyuan Li, Xuemin Yuan, Rongbing Tang, Jing Wang

“…The levels of Runx2 mRNA and ALP mRNA in the gingival epithelium were examined by quantitative real-time polymerase chain reaction. Results. LIRA decreased alveolar bone resorption, improved the microstructure of alveolar bone, and reduced periodontal inflammation and damage (P<0.05). …”
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Neuroprotective Effect of Wharton’s Jelly-Derived Mesenchymal Stem Cell-Conditioned Medium (WJMSC-CM) on Diabetes-Associated Cognitive Impairment by Improving Oxidative Stress, Neu... by Zohre Aghaei, Narges Karbalaei, Mohammad Reza Namavar, Masoud Haghani, Mahboobeh Razmkhah, Mahdi Khorsand Ghaffari, Marzieh Nemati

“…The expression levels of Bax, Bcl-2, BDNF, and TNF-α were detected by real-time polymerase chain reaction (RT-PCR). According to our findings, WJMSC-CM significantly reduced and increased blood glucose and insulin levels, respectively. …”
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Seasonal variations of microbial community in a full scale oil field produced water treatment plant by Q. Xie, S. Bai, Y. Li, L. Liu, S. Wang, J. Xi

“…The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. …”
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Navigating uncertainty in environmental DNA detection of a nuisance marine macroalga. by Patrick K Nichols, Kaʻuaʻoa M S Fraiola, Alison R Sherwood, Brian B Hauk, Keolohilani H Lopes, Colt A Davis, James T Fumo, Chelsie W W Counsell, Taylor M Williams, Heather L Spalding, Peter B Marko

“…We verified the eDNA assay using site-occupancy detection modeling from quantitative polymerase chain reaction (qPCR) data, calibrated with visual estimates of benthic cover of C. tumulosa that ranged from < 1% to 95%. …”
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Effect of Porous Chitosan Microspheres Loaded with Platelet-Rich Plasma and Bone Marrow-Derived Mesenchymal Stem Cells on Regeneration of Tibia Defect by Qi Chen, Ziru Zhao, Guoyong Yin

“…MTT assay was used to detect the proliferative ability of BMSCs after hypoxia/reoxygenation (H/R) treatment and the proliferative ability of BMSCs loaded by PCMs; polymerase chain reaction (PCR) was used to detect the expression of alkaline phosphatase (ALP), type I collagen (Col I), and type II collagen (Col II) in BMSCs after hypoxia and in BMSCs induced by PRP-loaded PCMs; PCR was used to detect the expression of Runt-associated transcription factor 2 (Runx2) and osteocalcin (OC) in the newly generated bone tissue; micro-CT scanning was applied to measure the bone mineral density and bone volume of the newly generated bone tissue in rats. …”
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Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY by Paulo C. Carvalho-Filho, Lilia F. Moura-Costa, Ana C. M. Pimentel, Mabel P. P. Lopes, Sibelle A. Freitas, Patrícia M. Miranda, Ryan S. Costa, Camila A. V. Figueirêdo, Roberto Meyer, Isaac S. Gomes-Filho, Teresa Olczak, Márcia T. Xavier, Soraya C. Trindade

“…PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. …”
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Infection Dynamics of Severe Acute Respiratory Syndrome Coronavirus-2 Wild-type and Delta Variant of Concern in a Pediatric Cohort in Southern India by Asha Jyothi Penugonda, Deepayan Biswas, Murugan Thimiri Palani, Sangeetha Priya, Rajesh Kumar Subaschandrabose, Koshy Alan Valiaveetil, Belavendra Antonisamy, Rajeev Zachariah Kompithra, Leni Grace Mathew, Mahesh Moorthy

“…Materials and Methods: Between November 2020 and August 2021, we enrolled children under 16 years testing positive for SARS-CoV-2 by polymerase chain reaction (PCR). We compared virus characteristics (viral load, virus variant - Wild-type or Delta), disease severity (asymptomatic, mild-to-moderate, and severe), and immunity levels to the virus (anti-N and anti-S) after 4–6 weeks. …”
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Differentiation value of miR-26b for major depressive disorder, schizophrenia, generalized anxiety disorder by Lingming Kong, Xiaoli Zhu, Liyi Zhang, Shengdong Chen

“…Quantitative real-time reverse transcription polymerase chain reaction was used to further verify aberrant miRNAs of previous identified in MDD and investigate expression level of these peripheral miRNAs in SZ and GAD. …”
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Relationship between Metabolic Syndrome Components and COVID-19 Disease Severity in Hospitalized Patients: A Pilot Study by Hande Erman, Banu Boyuk, Meltem Sertbas, Ali Ozdemir

“…The study population consisted of inpatients who tested positive by the reverse transcription polymerase chain reaction (RT-PCR) for SARS-CoV-2. Patients with critical COVID-19 disease on admission were excluded. …”
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Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil by Xiaoyan Zhao, Shu He, Rui Rui, Jingying Hei, Xiahong He, Shu Wang

“…Therefore, assessing the variability in NFB due to the cultivation of sanqi in the SPA system becomes crucial.MethodsThe seasonal dynamics in the abundance, diversity, and community structure of NFB in the soil of monocropping pine (MP) and SPA systems were assessed using real-time quantitative polymerase chain reaction and high-throughput sequencing technology.Results and discussionSanqi cultivation triggered a decrease in the abundance of NFB but increased α diversity. …”
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Next generation sequencing of multiple SARS-CoV-2 infections in the Omicron Era by Marzia Cavalli, Giulia Campoli, Anna Anselmo, Rossella Brandi, Antonella Fortunato, Maria Di Spirito, Anella Monte, Martina Lipari, Manfredo Bortone, Vanessa Vera Fain, Raffaele D’Amelio, Florigio Lista, Silvia Fillo

“…This analysis involved Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR) and Next Generation Sequencing (NGS). …”
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Therapeutic effect of selective interleukin-2-inducible tyrosine kinase inhibitor in orbital fibroblasts from patients with Graves’ orbitopathy by Yeonjung Yoon, Hyun Young Park, Min Kyung Chae, Sun Young Jang, Jin Sook Yoon

“…ITK mRNA expression in orbital tissues from GO and normal controls was compared using real-time polymerase chain reaction (RT-PCR) and immunohistochemistry. …”
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Association of functional variants in miRNA genes with the risk of coronary heart disease by Taqweem Ul Haq, Muhammad Riaz Khan, Sajjad Ali, Tariq Aziz, Thamer H. Albekairi, Aftab Ali Shah

“…Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction (T-ARMS-PCR) was used for genotyping, and Sanger sequencing was applied for validation in selected cases. …”
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Aetiology of haemorrhagic cystitis: BK Polyomavirus and Adenovirus detection by Yoliswa Z. Chili, Menzi B. Nkosi, Nicky-Louise Byrne, Moepeng J. Maseko, Stephen N.J. Korsman, Gert U. Van Zyl

“…During chemotherapy cycles, quantitative polymerase chain reaction (PCR) assays found high HAdV load, similar to a HAdV-B2 genotype 78, a rare genotype that is closely related to HAdV-11. …”
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Polymorphism T3111C of the Clock gene in ethnic groups of women from Russia and Buryatia by O. V. Kaluzhnaya, N. V. Semenova, T. A. Bairova, I. M.  Madaeva, E. V. Belyaeva, L. I. Kolesnikova

“…To this end, we conducted genotyping of DNA samples by polymerase chain reaction. It was shown that the compared groups have statistically significant differences in genotypes frequency (р = 0.001). …”
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Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. by Sarah I. Khaleel, Jaffar N. AlAlsaidissa

“…Expression analysis of MicroRNA-155-5p was performed using reverse transcription-quantitative polymerase chain reaction (qRT-PCR) on plasma isolated from peripheral blood. …”
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UCH-L1 Inhibitor Alleviates Nerve Damage Caused by Moyamoya Disease by Minghua Xu, Xiaomin Zhao, Jiang Zhao, Zhisheng Tan, Chengshi Zhang, Yun Huang, Huiping Zhong, Meifeng Guo, Chen Zhang, Ping Ye, Wentao Zheng

“…The total RNA was extracted from the exosomes, and the level of GFAP and UCH-L1 between the serum exosomes of the two groups was analyzed by a quantitative reverse transcription-polymerase chain reaction and western blot. Exosome labeling and uptake by SH-SY5Y cells were observed by confocal laser microscopy. …”
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