PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression by Caroline N. Rivera, Jason S. Hinkle, Rachel M. Watne, Trent C. Macgowan, Andrew J. Wommack, Roger A. Vaughan

“…Metabolic gene and protein expression were assessed via quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. …”
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Efficacy of vaccines based on chimeric or multiepitope antigens for protection against visceral leishmaniasis: A systematic review. by Karine Ferreira Lopes, Mariana Lourenço Freire, Silvane Maria Fonseca Murta, Edward Oliveira

“…Limiting dilution was the most used method for assessing parasite load, followed by quantitative real-time polymerase chain reaction (qPCR). Most domains had an uncertain risk of bias due to insufficient information described.…”
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Preamplification Procedure for the Analysis of Ancient DNA Samples by Stefania Del Gaudio, Alessandra Cirillo, Giovanni Di Bernardo, Umberto Galderisi, Theodoros Thanassoulas, Theodoros Pitsios, Marilena Cipollaro

“…Real-time quantitative polymerase chain reaction (qPCR) was used to detect in the extracts the presence of PCR inhibitors and to estimate the DNA content. …”
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The external validity of machine learning-based prediction scores from hematological parameters of COVID-19: A study using hospital records from Brazil, Italy, and Western Europe. by Ali Safdari, Chanda Sai Keshav, Deepanshu Mody, Kshitij Verma, Utsav Kaushal, Vaadeendra Kumar Burra, Sibnath Ray, Debashree Bandyopadhyay

“…The gold standard for COVID-19 detection, quantitative-Real-Time-Polymerase-Chain-Reaction (qRT-PCR), is expensive and time-consuming. …”
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Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses by Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Koji Nomoto, Nobuhiko Okada

“…The predominant species in the growing colonies were identified using colony polymerase chain reaction, and the colonies were counted.ResultsThe vaginal microbiota was classified into four categories based on the characterization of the dominant bacterial population: Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, and a diversity group. …”
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EZH2 Might Affect Macrophage Chemotaxis and Anti-Inflammatory Factors by Regulating CCL2 in Dental Pulp Inflammation by Ziqi Hu, Yingyi Chen, Jie He, He Liu, Tianqian Hui

“…The expressions of anti-inflammatory cytokine interleukins (IL-4 and IL-10) and transforming growth factor-β (TGF-β) in HDPCs which were treated by EZH2 complex, CCL2 complex, and CCL2 antibody were examined by quantitative real-time polymerase chain reaction (q-PCR). Results. The expression of TNF-α gradually increased in dental pulp inflammation. …”
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Plasma mcfDNA Sequencing May Improve Usual Care Diagnostics to Detect HHV-8 Among Outpatient People with Advanced HIV by Sarah Park, Brian Epling, Morgan Richey, Daniel Lupu, Mona Mughar, Irini Sereti

“…Yet, diagnosing KS can be challenging given the diverse manifestations and current limited usual care diagnostic methods (UC; polymerase chain reaction, histopathology). Methods: Pathogen-agnostic plasma microbial cell-free DNA sequencing was applied to banked samples from 116 outpatients included in 2 previous prospective studies of patients with antiretroviral treatment-naïve, advanced HIV (CD4 count ≤100 cells/µL). …”
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Geospatial Analysis and Molecular Epidemiologic Study of Emerging Pulmonary Lophomoniasis in Iran: A National Registry-Based Study by Mohammad Amin Ghatee, Maryam Nakhaei, Ali Sharifpour, Mahdi Fakhar, Niloufar Mohamadi, Mostafa Soleymani, Siavash Abedi, Masoud Aliyali, Hossein Mehravaran

“…They were tested for BPL by a newly developed polymerase chain reaction test. The study assessed the association of Normalized difference vegetation index (NDVI), digital elevation model (DEM), and geographic latitude as environmental factors and sex and age as demographic factors on the prevalence of BPL. …”
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Dataset for comparative analysis of precision metagenomics and traditional methods in urinary tract infection diagnosticsNCBI by Rob E. Carpenter, Sadia Almas, Vaibhav K. Tamrakar, Rahul Sharma

“…This study presents a comprehensive dataset comparing three diagnostic methodologies—microbial culture, polymerase chain reaction (PCR), and precision metagenomics (precision metagenomics)—for the detection and classification of uropathogens in urine samples from patients with suspected urinary tract infections (UTIs). …”
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Factors in Vaccine Refusal by Patients Applying for COVID-19 PCR Test by Ferhat ARSLAN, Behcet AL, Gorkem Alper SOLAKOGLU, Omer Faruk GULSOY, Cagatay NUHOGLU, Sema AYTEN

“…Objective: This study was designed to determine the reasons for vaccine rejection in patients who applied for the coronavirus disease-2019 (COVID-19) polymerase chain reaction (PCR) test but did not receive the vaccine. …”
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Polydatin ameliorates early brain injury after subarachnoid hemorrhage through up-regulating SIRT1 to suppress endoplasmic reticulum stress by Yuwei Han, Guangzhi Hao, Song Han, Tingzhun Zhu, Yushu Dong, Ligang Chen, Xinyu Yang, Xiaoming Li, Hai Jin, Guobiao Liang

“…Western blot and quantitative real-time polymerase chain reaction (PCR) analyses were performed to examine protein and mRNA expressions related to ER stress. …”
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Association between the aromatase (CYP19A1) gene variant rs10046 and cardiovascular risk in postmenopausal women by Betânia Rodrigues dos Santos, Gislaine Casanova, Thais Rasia da Silva, Karen Oppermann, Poli Mara Spritzer

“…Genotyping of the rs10046 polymorphism of the CYP19A1 gene was carried out using real-time polymerase chain reaction with allelic discrimination assays. …”
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Rational Primer and Probe Construction in PCR-Based Assays for the Efficient Diagnosis of Drifting Variants of SARS-CoV-2 by Divya RSJB Rana, Nischal Pokhrel, Santosh Dulal

“…The construction of polymerase chain reaction (PCR) assays for the reliable, efficient, and specific diagnosis of the drifting variants of SARS-CoV-2 is specifically governed by the selection and construction of primers and probes. …”
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The Anti-proliferative Effect of a Novel Glutaminase Inhibitor IN-3 on Prostate Cancer Cells by Ummuhan DEMIR, Ayse Busranur CELIK

“…The expression levels of GLS isoforms were determined by real-time polymerase chain reaction after IN-3 treatment. The metastatic prostate cancer dataset was downloaded from C-Bioportal and the expressions of GLS isoforms were analyzed. …”
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Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR by Mahsa Sadat Hashemi Doulabi, Reza Goleyjani Moghaddam, Ali Salehzadeh

“…This MDM2 polymorphism was investigated in DNA samples (extracted from biopsy samples) by amplification-refractory mutation system polymerase chain reaction. The mean age of patients with ulcerative colitis was 46.5 years (range, 28 to 69 years) and that of control individuals was 45.3 years (range, 26 to 71 years). …”
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Pleiotropic effects of rabeprazole at acid-related diseases by A. V. Zaborovsky, I. V. Mayev, D. N. Andreyev, L. A. Tararina

“…Genotyping of IL-1 gene (rs16944) polymorphisms, IL-6 gene (rs1800795), IL-10 gene (rs1800896) was carried out by polymerase chain reaction with allele discrimination by TaqMan-probes. …”
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Dysregulation of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in obese people and their predictive values for metabolic syndrome by Chenxi Yang, Yi Chen, Guangfeng Tang, Tongtong Shen, Li Li

“…Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the messenger RNA levels of JUN and FOSB in the peripheral blood of healthy volunteers (lean and obese) and patients with MetS (lean and obese), along with that in the adipose tissue and peripheral blood of obese mouse model. …”
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Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens by Ji Yun Bae, Ina Yun, Kang Il Jun, Chung-Jong Kim, Miae Lee, Hee Jung Choi

“…A. baumannii isolated from respiratory specimens was examined for the expression levels of ompA, uspA, hfq, hisF, feoA, and bfnL by quantitative reverse-transcription polymerase chain reaction. Among 156 patients with CRAB from respiratory specimens, 17 and 24 met the criteria for inclusion in the pneumonia and colonization groups, respectively. …”
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Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer by Jong-Lyul Park, Mirang Kim, Kyu-Sang Song, Seon-Young Kim, Yong Sung Kim

“…We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. …”
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Metformin Exhibits Anti‐Inflammatory Effects by Regulating microRNA‐451/CXCL16 and B Cell Leukemia/Lymphoma 2 in Patients With Osteoarthritis by Nahid Alimoradi, Amin Ramezani, Mohammad Tahami, Negar Firouzabadi

“…In addition to evaluating the clinical response using the Knee Injury and Osteoarthritis Outcome Score questionnaire, miR‐451 and miR‐15b expression levels were detected using real‐time polymerase chain reaction. The serum levels of CXCL16 and BCL‐2 were evaluated using enzyme‐linked immunosorbent assay kits before (time zero) and after treatment (month four). …”
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