Dengue virus infection among renal transplant recipients in Singapore: a 15-year, single-centre retrospective review by Sophie Seine Xuan Tan, Quan Yao Ho, Sobhana Thangaraju, Thuan Tong Tan, Terence Kee, Shimin Jasmine Chung

“…The diagnosis of dengue was made if there were a compatible clinical syndrome and a positive dengue diagnostic assay (dengue non-structural 1 antigen, immunoglobulin M or reverse transcriptase-polymerase chain reaction). Results: Of the 31 patients diagnosed with dengue, 18 (58.1%) were deceased donor recipients. …”
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Etiopathogenesis of Sheehan’s Syndrome: Roles of Coagulation Factors and TNF-Alpha by Halit Diri, Elif Funda Sener, Fahri Bayram, Nazife Tascioglu, Yasin Simsek, Munis Dundar

“…PAI-1 4G/5G and TNF-α (-308  G>A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. …”
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Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran by Danial Jahantigh, Saeedeh Salimi, Roya Alavi-Naini, Abolfazl Emamdadi, Hamid Owaysee Osquee, Farzaneh Farajian Mashhadi

“…The TLR4 genes Asp299Gly, Thr399Ile, and TLR9 gene T-1486C polymorphisms were amplified by polymerase chain reaction (PCR) and then detected by PCR-restriction fragment length polymorphism (RFLP). …”
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POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC by Ryana Budi Purnama, Setiadi Warata Logamarta, Agung Dhartono

“…The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. …”
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A Pilot Seroprevalence Study Suggests Silent Zika virus Transmission in Bhopal Region of Central India by Shashwati Nema, Dipesh Kale, Romesh Jain, Ajay Halder, Manisha Shrivastava, Deepak Vaishnav, Ashvini Kumar Yadav, Divya Namdeo, Debasis Biswas

“…We tested blood samples of febrile patients for ZIKV RNA using real-time polymerase chain reaction (PCR), and for the healthy individuals, we determined anti-zika immunoglobulin G (IgG) antibodies using enzyme-linked immunosorbent assay. …”
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Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells by Yoshinori Shinohara, Shuhei Tsuchiya, Kazuo Hatae, Masaki J. Honda

“…The subcultured EOE cells retained their capacity for ameloblast-related gene expression, as shown by semiquantitative reverse transcription-polymerase chain reaction. Amelogenin expression was detected in the subcultured EOE cells by immunostaining. …”
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Autophagy Mediates MMP-2 Expression in Glaucomatous Trabecular Meshwork Cells by Yan-Ling Xiao, Xiao-Rui Wang, Kejing Ye, Wan-Zhu Chen, Bing-Ru Zheng, Yi-Hong Huang, Yu-Yu Wu

“…The mRNA and protein expression of LC3-II, Beclin1, and MMP-2 were measured by reverse transcription-polymerase chain reaction and western blot, respectively. Results. …”
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The Association between Platelet Glycocalicin and High Microsatellite Instability in Colorectal Cancer by Zeng-yao Liu, Qing-chun Jia, Wen Wang, Yu-xi Liu, Rui-tao Wang, Jia-yu Li

“…The microsatellite status was determined with a polymerase chain reaction. The participants were separated into high microsatellite instability (MSI-H) and microsatellite stable (MSS) groups according to microsatellite status. …”
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Laser Capture Microdissection in Dentistry by Uraiwan Chokechanachaisakul, Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda, Jacques E. Nör

“…For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. …”
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Study of some virulence genes from uropathogenic Klebsiella pneumoniae and Pseudomonas aeruginosa isolated in Wasit Province, Iraq by Rana H. Raheema, Sabaa Hilal Hadi, Zainab Adil Ghani Chabuck

“…These findings were validated by the polymerase chain reaction method used to detect the virulence genes fimH gene, where 4/16 (25%) and mrkA gene, where result 7/16 (43.75%) were positive in recovered K. pneumoniae isolated. …”
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The Frequency Of Hematological Abnormalities in Children (Age 1 Month To 12 Years) Presenting With Sars-Cov-2 Infection At Shifa International Hospital by Sidra Tul Muntaha, Hammad Ayaz, Huma Mahmood, Afifa Kulsoom, Muhammad Tayyab, Arslan Farooq

“…A total of 325 pediatric patients of 1 month to 12 years of age who presented with symptoms of COVID-19 having positive reverse transcription polymerase chain reaction results were included in this study. …”
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Preservation of Aquatic Environmental DNA Using Cationic Detergents by Viresh Thamke, Yared H. Bezabhe, Jana Jass, Per‐Erik Olsson

“…Analysis by quantitative polymerase chain reaction (qPCR) demonstrated a mtDNA retention rate of 17.6%, 26.3%, and 2.2% for CPC, CTAB, and BAC, respectively, compared to 0.1% in untreated water after 30 days. …”
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Transcription Factor FXR Activates DHRS9 to Inhibit the Cell Oxidative Phosphorylation and Suppress Colon Cancer Progression by Jinlai Zhao, Yigang Wang, Yang Wang, Jianchao Gao, Haichao Yang, Xiaotang Wu, Hua Li

“…The mRNA and protein expression of FXR in colon cancer cells were measured by quantitative real-time polymerase chain reaction and Western blot. The effects of FXR on the biological function of colon cancer cells were measured by Cell Counting Kit-8, colony formation, and transwell assays. …”
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Species identification based on the fecal DNA samples of the Caprinae by Abdurakhim E. Kuchboev, Oybek O. Amirov, Makhamadi B. Abramatov, Bakhtiyor H. Ruziev, Mehmonjon Kh. Egamberdiyev, Rokhatoy R. Karimova

“…We used species-specific 16S rRNA mitochondrial gene fragments for polymerase chain reaction amplification for species identification. …”
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Multiplex-PCR technique to predict polymorphic antigens – M, N, S and s – and associations between their alleles and Mia-associated hybrid glycophorins by Thanaporn Kerdthip, Oytip Nathalang, Sarisa Chidtrakoon, Tanaporn Choychimplee, Dollapak Apipongrat, Kamphon Intharanut

“…Alternatively, multiplex polymerase chain reaction (PCR) is a commonly used genotyping tool due to its potency and ability to amplify several DNA targets simultaneously in a single reaction. …”
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Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates by Kadhim Jwad Jamin, Hussein O. M. Al-Dahmosi, Sura Ihsan Abed Jabuk

“…The antibiotics susceptibility test was carried out by using 12 antibiotics from different classes The molecular identification was done by using polymerase chain reaction amplification of the three bla CTX-M gene (bla CTX-MI, bla CTX-MIV, bla CTX-MII(. …”
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The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway by Nana Wang, Tiegang Li, Ping Han

“…Area under the curve (AUC) and homeostatic model assessment of insulin resistance (HOMA-IR) were calculated, and PI3-K/AKT signal pathway-related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis in muscle, adipose, and liver tissues, respectively. …”
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Association of Human Leukocyte Antigen Class I Polymorphism with Spontaneous Clearance of Hepatitis B Surface Antigen in Qidong Han Population by Fengqin Miao, Hang Sun, Ning Pan, Jinhuan Xu, Jie Qiu, Yuqing Shen, Wei Xie, Jianqiong Zhang

“…We genotyped HLA-A, -B, and -C loci of 448 individuals with HBV persistent infection and 140 persons with spontaneous clearance of HBsAg by polymerase chain reaction with sequencing based typing (PCR/SBT). …”
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Prevalence of Trypanosoma congolense and Trypanosoma vivax in Lira District, Uganda by Aziz, Katabazi, Adamu, Almustapha Aliero, Simon Peter, Musinguzi

“…This study was designed to compare the prevalence of animal African trypanosomes in cattle in Lira District using microscopy and polymerase chain reaction amplification (PCR) methods. In this cross-sectional study, a total of 254 cattle from the three villages of Acanakwo A, Barropok, and Acungkena in Lira District, Uganda, were selected by simple random sampling technique and screened for trypanosomiasis using microscopy and PCR methods. …”
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Identification of the BBX gene family in blueberry at different chromosome ploidy levels and fruit development and response under stress by Yujian Xue, Jiazhuo Chen, Jia Hao, Xiaoyu Bao, Luodan Kuang, Dong Zhang, Chengwen Zong

“…The analysis of transcriptome and quantitative reverse transcription–polymerase chain reaction data revealed that the expression pattern of BBX genes depended on the developmental stage of blueberry fruit. …”
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