First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants by Nagwa Eisawi, Jabbar Ahmed, Mohammed A. Bakheit, Dina A. Hassan, Mohammed O. Hussien, Abdel Rahim M. El Hussein

“…This study aimed to detect and characterize spotted fever group (SFG) rickettsiae in ixodid ticks infesting domestic ruminants in Khartoum State. Methods Polymerase chain reaction targeting both genes that encode for citrate synthase (gltA) and outer membrane protein (ompA) was performed for the presence of SFG rickettsia followed by sequence and phylogenetic analysis. …”
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Role of HLA alleles polymorphism in systemic lupus erythematosus: A prospective study from North India by Ranjan S. Rana, Bitan Naik, Mahima Yadav, Usha Singh, Anup Singh, Shailja Singh

“…HLA typing was done by polymerase chain reaction (PCR)-sequence-specific oligonucleotide (SSO) method (SSO probe). …”
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Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains by C. Mary, F. Faraut, M. Deniau, J. Dereure, K. Aoun, S. Ranque, R. Piarroux

“…Relative quantification experiments by means of real-time polymerase chain reaction showed that multidrug resistance gene amplification is the more frequent event. …”
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Genetic tracking of Crimean-Congo haemorrhagic orthonairovirus in Hyalomma population infesting cattle in Nigeria. by Oluwafemi Babatunde Daodu, Joseph Ojonugwa Shaibu, Rosemary Ajuma Audu, Daniel Oladimeji Oluwayelu

“…The CCHFV S-segment was detected using specific primers via reverse transcriptase polymerase chain reaction, followed by sequencing of amplicons. …”
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CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens by Md. Rashidur Rahman, Toma Rani Majumder, Md. Aminul Islam Apu, Alok K. Paul, Afrina Afrose, Biplab Kumar Dash

“…However, standard pathogen detection methods, including culture and microscopic observation, antibody-based serologic tests, and primarily polymerase chain reaction (PCR)-oriented nucleic acid screening techniques, have shortcomings that limit their widespread use in responding to outbreaks and regular diagnosis, especially in remote resource-limited settings (RLSs). …”
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An Inflammatory Pseudotumor Arising from Pterygopalatine Fossa with Invasion to the Maxillary Sinus and Orbital Cavity by Hidenori Yokoi, Takuya Yazawa, Yuma Matsumoto, Tetsuya Ikeda, Masachika Fujiwara, Yasuo Ohkura, Naoyuki Kohno

“…To obtain a definitive diagnosis from histopathological analysis, the lesion was biopsied, and she was diagnosed as the inflammatory pseudotumor with the immunohistochemical study and multiplex polymerase chain reaction-based clonality assays. The patient had a lymphoid-predominant lesion that responded to radiotherapy but corticosteroids were not effective. …”
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Investigation of genetic structure of various cat breeds by using d-loop polimorphism in turkey by Elif Yılmaz Şahin, Vahdettin Altunok, Ercan Kurar

“…The products of Polymerase Chain Reaction (PCR) CEQ-8000 are determined by using the System of Genetic Analysis of Beckman Coulter and sequence of sequences resoluted with capillary electrophoresis are determined.…”
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A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii... by Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani

“…Also, IUS genes were detected by the polymerase chain reaction (PCR) method. Results. Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. …”
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Dietary Quercetin Regulates Gut Microbiome Diversity and Abundance in <i>Apis cerana</i> (Hymenoptera Apidae) by Haodong Wu, Conghui Ji, Ruisheng Wang, Lijiao Gao, Wenhua Luo, Jialin Liu

“…Gut samples were collected on the 5th and 9th days, subjected to a polymerase chain reaction and 16S rRNA sequencing, and analyzed. …”
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B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts by Qiao Kang, Wenhao Wang, Shengtao Wu, Ganggang Hu

“…Western blotting for protein expression detection together with real-time quantitative polymerase chain reaction for mRNA expression measurement were carried out on those related to hBMSC proliferation and differentiation. …”
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Clinical Performance of Rapid Antigen Tests for the Detection of SARS-CoV-2 Infection in the Emergency Department and Community: A Retrospective Study by Pei-Chin Lin, Hung-Pin Chiu, Fang-Yi Cheng, Chih-Chun Chang, Fang-Yeh Chu

“…This study aimed to provide evidence regarding the diagnostic performance of SARS-CoV-2 rapid antigen tests compared with the real-time reverse transcription-polymerase chain reaction (RT-PCR) test in the emergency department (ED) and community. …”
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Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from Milk in Shire Dairy Farms, Tigray, Ethiopia by Weldemelak Girmay, Getachew Gugsa, Habtamu Taddele, Yisehak Tsegaye, Nesibu Awol, Meselu Ahmed, Aklilu Feleke

“…Furthermore, multiplex polymerase chain reaction (PCR) was done for the detection of mec A and fem A genes. …”
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Genetic Analysis Reveals A Missense Mutation In Reactivation Gene-2 Causing Severe Combined Immunodeficiency Disease In A Pakistani Family by Shahida Awais, Lubna Siddique, Nida Shafi, Sobia Humerah, Summyah Niazi, Wafa Omer

“…Exons specific polymerase chain reaction (PCR) was performed using oligonucleotide primers and subsequently DNA Sanger sequenced which resulted into an missense variant in the gene RAG2. …”
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Tumor Necrosis Factor-Alpha Gene Promoter Region Polymorphism and the Risk of Coronary Heart Disease by Gul Zareen Asifa, Afrose Liaquat, Iram Murtaza, Syed Ali Raza Kazmi, Qamar Javed

“…Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). …”
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Molecular identification and genetic diversity analysis of Cryptosporidium spp. infecting dogs from central and northern Jordan: Detection of zoonotic genotype IId. by Rami M Mukbel, Eman M Etoom, Haifa B Hammad, Heidi L Enemark, Marwan M Abu Halaweh

“…Faecal samples were screened for Cryptosporidium using polymerase chain reaction (PCR) targeting the 18S rRNA gene, revealing an overall infection rate of 18.9% (47 out of 249). …”
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LncRNA MANCR is downregulated in non-small cell lung cancer and predicts poor survival by Yunming Tao, Jie Liu, Wenxiao Qiu, Yuanyuan Li

“…Methods After NSCLC (n = 60) and control (healthy subjects, n = 60) plasma samples, as well as NSCLC and paired non-tumor tissues from patients were collected, the levels of MANCR expression in plasma and tissues was detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Then the correlations of MANCR expression with clinical stages were confirmed. …”
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Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass by Yibin Yang, Ping Deng, Hao He, Qiuhong Yang, Xiaohui Ai, Xia Zhu

“…Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes simA, pdi, sagA, and cfi. …”
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Association of XRCC3, XRCC4, BAX, and BCL-2 Polymorphisms with the Risk of Breast Cancer by Emre Ozoran, Fadime Didem Can Trabulus, Duygu Erhan, Bahadir Batar, Mehmet Guven

“…Gene polymorphisms were detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. 175 patients and 158 healthy controls were enrolled in the study. …”
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Fatal Multiorgan Failure Associated with Disseminated Herpes Simplex Virus-1 Infection: A Case Report by Michael Glas, Sigrun Smola, Thorsten Pfuhl, Juliane Pokorny, Rainer M. Bohle, Arno Bücker, Jörn Kamradt, Thomas Volk

“…Post-mortem polymerase chain reaction analyses confirmed a disseminated infection with HSV-1 involving various organs and tissues but excluding the central nervous system. …”
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Streptomyces sp. MUM 195J: A Promising Probiotic for Controlling Vibrio parahaemolyticus Infection in Aquaculture by Joanna Xuan Hui Goh, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Giva Kuppusamy, Janaranjani M, Kooi-Yeong Khaw, Wei Hsum Yap, Kok-Gan Chan, Vengadesh Letchumanan, Learn-Han Lee, Bey-Hing Goh

“…Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed that probiotic supplementation elevated the immune function of the animal. …”
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