Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction by Reza Yesica, Andreas Bandang Hardian, Yudit Oktanella, Shelly Kusumarini, Gabriela Jeanne Giacynta Prasetyo

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Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments by Robert B. Page, Arnold J. Stromberg

“…Relative expression quantitative real-time polymerase chain reaction (RT-qPCR) experiments are a common means of estimating transcript abundances across biological groups and experimental treatments. …”
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Development of polymerase chain reaction kit for detection of SARS-CoV-2 RNA in biological samples collected from animals by A. M. Timina, A. S. Yakovleva, M. V. Timanov, M. V. Biryuchenkova, Ye. S. Orlova

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A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid by Tatjana Luzius, Samuel D. Jeske, Julia Baer, Uta Goelnitz, Ulrike Protzer, Jochen M. Wettengel

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Positive real-time PCR in pneumococcal meningitis 12 hours after initiation of antibiotic therapy – case report by Cem Thunstedt, Carla Palleis, Johannes Wischmann, Suzette Heck, Konstantinos Dimitriadis, Matthias Klein

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Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

Subjects: “…novel reverse transcriptase polymerase chain reaction…”
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Identification of Common Bacterial Pathogens Causing Meningitis in Culture-Negative Cerebrospinal Fluid Samples Using Real-Time Polymerase Chain Reaction by Walaa Shawky Khater, Safia Hamed Elabd

“…Forty culture-negative CSF samples from suspected cases of bacterial meningitis were examined by real-time polymerase chain reaction (real-time PCR) for the presence of lytA, bexA, and ctrA genes specific for Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, respectively. …”
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Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem by James Ian Stuart, Johan Delport, Robert Lannigan, George Zahariadis

“…BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. …”
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METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS by M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, A. V. Borisov

“…Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. …”
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Impact of viral load on sample pooling for reverse-transcription polymerase chain reaction detection-based diagnosis of coronavirus disease 2019 in Nigeria by Timan T. Eliya, Elvis E. Isere, Bassey Emmana, Chukwuebuka Ugwu, Jonathan Kushim, Precious Ishaku, Aisha E. Ibrahim, John S. Bimba

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Clinical Presentation of Patients with Aseptic Meningitis, Factors Influencing Treatment and Hospitalization, and Consequences of Enterovirus Cerebrospinal Fluid Polymerase Chain Reaction Testing by Glenn Patriquin, Jill Hatchette, Kevin Forward

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Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction by Chen Chen, Jia Chen, Yan Zhang, Yongbo Li, Zan Wang, Xian Li, Wei Zhou, Zhisheng Zhang

“…A single-copy specific primer was designed based on beef and duck samples and through drop digital polymerase chain reaction (ddPCR) for the quantitative analysis. …”
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METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS by M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, А. V. Borisov

“…Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. …”
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Detection of new single nucleotide polymorphisms in plasma cells carcinoma (myeloma) by polymerase chain reaction–single strand conformation polymorphism and sequencing technical methods by Sarah K. Obayes, Sabah H. Enayah, Shaimaa A. Al-Oubaidy, Rana A. Ghaleb

“…Genotyping of miRNA was accomplished using the polymorphism techniques of polymerase chain reaction and single strand conformation polymorphism (SSCP). …”
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Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction by Mohammed Taha Ahmed Baban, Dena Nadhim Mohammad, Natheer Al-Rawi

“…This research aims to assess the precision of simultaneously amplifying both genes using multiplex polymerase chain reaction on samples derived from teeth that have been subjected to various forensic conditions. …”
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Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid by Yusaku Noma, Hayate Sawayama, Kouta Yamashiro, Keitaro Nishi, Haruka Yoshinaga, Ryota Takaoka, Tsuyoshi Sugiura, Tomonori Sasahira, Tomofumi Hamada, Yasuo Suda, Tatsuo Okui

“…We therefore sought to develop a simple screening method for the diagnosis of oral cancer by conducting a melting curve analysis in a quantitative real-time polymerase chain reaction (qPCR). The assay was performed by a qPCR of DNA from the gargle fluid of 20 patients with oral cancer and 20 healthy control subjects in the training set. …”
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eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers by Bo‐Young Lee, Grant A. Milne, Corwin Freedman, Jenifer Miksis‐Olds, Bonnie L. Brown

“…The available 12S rRNA fish universal primer analysis system (MiFish and 12S‐V5) was modified using nested polymerase chain reaction (PCR) to improve targeting of fish products and reduce non‐target products. …”
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Mutational Analysis of KCNQ1 Gene in Type 2 Diabetes Mellitus: A Case-control Study by Ankush Ganjewar, Ishwar B Bagoji, Sharan Badiger, Rudragouda B Bulagouda, Gurushnatappa Kadakol

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Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits by V. V. Evstifeev, F. M. Khusainov, S. I. Yakovlev, R. I. Shangaraev, V. I. Eremets

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Effect of the host-specific toxin SnTOX3 from Stagonospora nodorum on ethylene signaling pathway regulation and redox-state in common wheat by S. V. Veselova, G. F. Burkhanova, T. V. Nuzhnaya, S. D. Rumyantsev, I. V. Maksimov

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