Comparison of Different Phenotypic Tests versus PCR in the Detection of Carbapenemase-Producing Pseudomonas aeruginosa Isolates in Hamadan, Iran by Masoumeh Beig, Mohammad Taheri, Mohammad Reza Arabestani

“…We evaluated the performance of MHT, CarbaNP, CDDT, and CIM tests in comparison to polymerase chain reaction (PCR) for the detection of carbapenemase-producing isolates. …”
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Development of a conjunctival contact–type drug delivery device for latanoprost using hyaluronic acid by Soomin Lee, Mi-Young Jung, Choul Yong Park

“…On day 10, we quantified gene expression of inflammatory cytokines and IOP-affecting proteins using polymerase chain reaction, and performed histological and immunohistochemical analyses. …”
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lncRNA RUNDC3A-AS1 Regulates Proliferation and Apoptosis of Thyroid Cancer Cells via the miR-151b/SNRPB Axis by Yan Deng, Jie Wu, Xin Li

“…Relative expression levels of RUNDC3A-AS1, microRNA (miR)-151b, and small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB) were examined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) in THCA cells. The localization of RUNDC3A-AS1 in THCA cells was detected by subcellular fractionation assay. …”
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Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq by Sura S. Aghwan, Enas S. Hussein, Salam A Esmaeel

“…To achieve this goal, microscopic and conventional polymerase chain reaction technology was used, and the genetic analysis of Cytauxzoon spp. was verified. …”
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16S rRNA and Omp31 Gene Based Molecular Characterization of Field Strains of B. melitensis from Aborted Foetus of Goats in India by Ajay Singh, Vivek Kumar Gupta, Amit Kumar, Vikas Kumar Singh, Shivasharanappa Nayakwadi

“…All the isolates produced amplification products of 1412 and 720 bp in polymerase chain reaction with genus and species specific 16S rRNA and omp31 gene based primers, respectively. …”
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Glutathione S-transferase polymorphism in ethnic groups living in Eastern Siberia by E. V. Belyaeva, O. A. Yershova, T. A. Astahova, O. V. Bugun

“…To this end, genetic typing of DNA samples was performed by polymerase chain reaction. Statistically significant differences in the frequency of occurrence of alleles A, B, C of the GSTP1 gene (р = 0.026) were found between the groups of teenagers compared. …”
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Epithelial and immune transcriptomic characteristics and possible regulatory mechanisms in asthma exacerbation: insights from integrated studies by Ye Liu, Yue Li, Ruhao Wu, Yu Wang, Pengfei Li, Tianci Jiang, Ke Wang, Yize Liu, Zhe Cheng

“…Asthma animal and in vitro cell line models were established to verify the key regulatory genes expression by employing quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsScRNA-seq analysis identified 7 epithelial subpopulations and 14 distinct immune cell types based on gene expression profiles. …”
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New insights into the DNA extraction and PCR amplification of minute ascomycetes in the genus Laboulbenia (Pezizomycotina, Laboulbeniales) by Warre Van Caenegem, Danny Haelewaters

“…In particular, the genus Laboulbenia is notorious for low success with DNA extraction and polymerase chain reaction (PCR) amplification. This is attributed to the presence of melanin, a molecule known to inhibit PCR, in the cells. …”
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CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis by Jianqiang Wu, Lixia Chen, Peijun Wen

“…Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blots (WB) were used to detect stable overexpression and knockdown of CRIP1 in melanoma cells. …”
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A Patient with 38 Years of Smoking History and Severe Shortness of Breath by E. R. Buklis, N. I. Kokina, E. L. Bueverova, M. V. Feldsherov, V. S. Komarova, V. T. Ivashkin

“…The diagnosis was established: bilateral bronchiolitis associated with a new coronavirus infection (SARS-CoV-2 polymerase chain reaction — positive); chronic obstructive pulmonary disease stage II according to GOLD (Global Initiative for Chronic Obstructive Lung Disease), exacerbation. …”
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Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal by Upasana Ghimire, Rupesh Kandel, Mary Neupane, Sanjit Shrestha, Sudeep K C, Santosh Khanal, Dev Raj Joshi

“…The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test, and target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth, whereas 32.75% (19/58) isolates were identified as A. baumannii. …”
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Clonally Related Plasmablastic Lymphoma Simultaneously Occurring with Diffuse Large B-Cell Lymphoma by Norisato Hashimoto, Tomoki Ueda, Shinichiro Hiraiwa, Takuma Tajiri, Naoya Nakamura, Kenji Yokoyama

“…We extracted DNA from the two lymphomas for polymerase chain reaction and sequenced the amplified immunoglobulin heavy variable genes and the complementarity-determining region- (CDR-) 3. …”
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Exploring Unique Extracellular Vesicles Associated Signatures: Prognostic Insights, Immune Microenvironment Dynamics, and Therapeutic Responses in Pancreatic Adenocarcinoma by Kai Nan, Ming Zhang, Zilong Geng, Yuankai Zhang, Lin Liu, Zhi Yang, Peng Xu

“…Differential expression of selected lncRNAs in both cellular and exosomal fractions of PAAD was further confirmed through quantitative polymerase chain reaction (qPCR). Eight DELs were identified from TCGA and GTEx datasets, and two exosomal lncRNAs exhibited a significant correlation with overall survival, warranting further investigation. …”
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Blood Borne Viral Infections in Transplantation: Hepatitis Viruses and Retroviruses by RP Bryce Larke

“…Despite recent improvements in anti-HCV testing, current screening assays underestimate the incidence of transmission and prevalence of HCV infection among immunosuppressed organ recipients: evidence of ongoing HCV infection depends on detection of HCV RNA by polymerase chain reaction. Determination of I-IIV-1 p24 antigen may facilitate identification of prospective organ donors in ll1e window period of early infection and may enhance serological follow-up of allograft recipients al risk of transplantation-associated HIV-1 infection. …”
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Occurrence of Bordetella Infection in Pigs in Northern India by Sandeep Kumar, Bhoj R. Singh, Monika Bhardwaj, Vidya Singh

“…In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). …”
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Circulating Tumor DNA in a Breast Cancer Patient's Plasma Represents Driver Alterations in the Tumor Tissue by Jieun Lee, Sung-Min Cho, Min Sung Kim, Sug Hyung Lee, Yeun-Jun Chung, Seung-Hyun Jung

“…This mutation was subsequently validated by digital polymerase chain reaction in liquid biopsy. Our result confirm that driver alterations identified in the tumor tissue were detected in liquid biopsy by targeted NGS as well, and suggest that a higher depth of sequencing coverage is needed for detection of genomic alterations in a liquid biopsy.…”
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METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway by Jinghua Wang, Shushan Yan, Hongying Lu, Shufeng Wang, Donghua Xu

“…We used quantitative real-time polymerase chain reaction to detect the expression of METTL3 in RA patients and controls as well as the macrophage cell line. …”
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Varicella Zoster Virus Meningitis in a Young Immunocompetent Adult without Rash: A Misleading Clinical Presentation by Thomas Pasedag, Karin Weissenborn, Ulrich Wurster, Tina Ganzenmueller, Martin Stangel, Thomas Skripuletz

“…Further CSF analyses, including polymerase chain reaction (PCR) and detection of intrathecal synthesis of antibodies, showed a VZV infection. …”
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Antibiotic resistance gene occurrence in poultry farms in northeast Brazil by Henrique Francisco de Almeida, Paulo Ricardo Conceição Marques Trindade, César Roberto Viana Teixeira, Claudson Oliveira Brito, Silvio Santana Dolabella, Sona Jain, Maíra Pompeu Martins, Ana Andréa Barbosa

“…The presence of ARGs in the obtained material was tested by Polymerase Chain Reaction (PCR) using primers for selected ARGs. …”
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Progressive Multifocal Leukoencephalopathy in a Multiple Sclerosis Patient Diagnosed after Switching from Natalizumab to Fingolimod by Tim Sinnecker, Jalal Othman, Marc Kühl, Imke Metz, Thoralf Niendorf, Annett Kunkel, Friedemann Paul, Jens Wuerfel, Juergen Faiss

“…We report a MS patient in whom PML was highly suspected solely based on MRI findings after switching from NTZ to fingolimod despite repeatedly negative (ultrasensitive) polymerase chain reaction (PCR) testing for JC virus DNA in cerebrospinal fluid. …”
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