Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia by Ying Chen, Chenchen Zhou, Xiaobo Zhao, Ronghua Che, Yuelin Wu, Sheng Wan, Jinda Pei, Liping Yao, Xiaolin Hua

“…The messenger ribonucleic acid (mRNA) levels of miR-195 and tissue factor pathway inhibitor 2 (TFPI2) were measured in placental tissues derived from normal and preeclampsia patients by real-time polymerase chain reaction (PCR). Human umbilical cord mesenchymal stem cell (hUC-MSC)-derived extracellular vesicles were verified by western blot. …”
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Artificial Neural Network-Based Identification of Associations between UCP2 and UCP3 Gene Polymorphisms and Meat Quantity Traits by Javed Ahmed Ujjan, Sapna Waheed Memon, Majeeda Ruk, Zuneera Akram, Allahwadhayo Ghoto, Betty Nokobi Dugbakie

“…For better analysis, the output of the ANN model is loaded into the Primer Premier 3 software. Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing, the results of this study indicated 07 variations in the exon 4 region of the UCP2 gene and 03 variants in the exon 3 area of the UCP3 gene among 215 indigenous cow breeds. …”
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Identification of Mutations that Encode Drug Resistance in the Polymerase Gene of the Human Immunodeficiency Virus by Zhengxian Gu, Hengsheng Fang, Horacio Salomon, Qing Gao, Mark A Wainberg

“…The complete reverse transcriptase open reading frames of these viruses, and portions of flanking protease and integrase within the pol gene, were cloned and sequenced by polymerase chain reaction (PCR) techniques. Mulalions were observed at each of amino acid sites 65 (Lys → Arg: AAA → AGA) and 184 (Met → Val: ATG → GTG) when ddC was used in this protocol, and at site 184 only when either 3TC or ddl was employed. …”
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The Effect of Clopidogrel and Ticagrelor on Human Adipose Mesenchymal Stem Cell Osteogenic Differentiation Potential: In Vitro Comparative Study by Sally S. Mohamed, Hala F. Zaki, Shereen N. Raafat

“…Quantitative determination for osteogenic markers included osteocalcin (OC); runt-related transcription factor 2 (RUNX2) performed using western blot; osteoprotegerin (OPG) using enzyme-linked immunosorbent assay (ELISA) and inflammatory markers; and tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) measured using real-time polymerase chain reaction quantitative (RT-PCR) and ELISA. …”
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Expression Profiles of 12 Late Embryogenesis Abundant Protein Genes from Tamarix hispida in Response to Abiotic Stress by Caiqiu Gao, Yali Liu, Chao Wang, Kaimin Zhang, Yucheng Wang

“…The expression profiles of these genes in response to NaCl, PEG, and abscisic acid (ABA) in roots, stems, and leaves of T. hispida were assessed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). These ThLEAs all showed tissue-specific expression patterns in roots, stems, and leaves under normal growth conditions. …”
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Application of Next-Generation Sequencing Technology Based on Single Gene Locus in Species Identification of Mixed Meat Products by Xinmei Liu, Zhiyang Liu, Yiyu Cheng, Haijing Wu, Wei Shen, Yan Liu, Qiushi Feng, Jun Yang

“…Polymerase chain reaction (PCR) detection is a commonly used method for species identification of meat products. …”
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Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa by Sandeep Vasaikar, Larry Obi, Isaac Morobe, Mary Bisi-Johnson

“…The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of blaCTX-M, blaTEM, blaSHV, blaKPC, and blaNDM genes. …”
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Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning by P. Janczyk, R. Pieper, V. Urubschurov, K. R. Wendler, W. B. Souffrant

“…Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. …”
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Comparison of Prevalence of blaNDM-1 Gene among Metallo-Beta Lactamase-Producing Multidrug-Resistant Fermenters and Nonfermenters in a Tertiary Care Hospital by Shaveta Kataria, Shipra Galhotra, Mohit Kumar, Priyanka Thandi, Neerja Jindal

“…Combined disk synergy test (CDST) and polymerase chain reaction (PCR) analysis are used for the detection of MBL production. …”
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Endocervical adenocarcinomas and HPV genotyping in an HIV endemic milieu – a retrospective study by Lucília Lovane, Gabriella Lillsunde Larsson, Satish Tulsidás, Carla Carrilho, Sören Andersson, Christina Karlsson

“…HPV detection was performed using a multi-methodological approach, including Anyplex II, in-house polymerase chain reaction (PCR), and chromogenic and fluorescent in situ hybridisation techniques. …”
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Correlation between Follicular Fluid Fatty Acids and Cell-Free Mitochondrial DNA in Women Undergoing Intra-Cytoplasmic Sperm Injections by Zainab M. Alawad, Hanan L. Al-Omary

“…The samples were assessed for fatty acids, utilizing gas chromatography, and for relatively cell-free mitochondrial DNA, real-time polymerase chain reaction (PCR) was used. Results: There was a strong significant positive correlation between follicular fluid margaric acid and follicular fluid relative to cell-free mitochondrial DNA, as the correlation coefficient was 0.869, and the P value was 0.025. …”
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Downregulation of HBx Restrains Proliferation, Migration, and Invasion of HepG2 Cells by Chaoqun Huang, Wei Liu, Xiaochuan Zhao, Libin Zhao, Fuxiang Wang

“…HBx and XB130 expression was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot. Overexpression of HBx and XB130 was found in liver cancer tissues and cells. …”
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Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism and Small Vessel Cerebral Stroke in Indian Population by Puttachandra Prabhakar, Tanima De, Dindagur Nagaraja, Rita Christopher

“…ACE I/D polymorphism was detected by polymerase chain reaction. Results. Hypertension was significantly more prevalent in the patient group and was associated with 6-fold increase in risk for stroke. …”
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A Universal Analysis Pipeline for Hybrid Capture-Based Targeted Sequencing Data with Unique Molecular Indexes by Min-Jung Kim, Si-Cho Kim, Young-Joon Kim

“…Unique molecular index (UMI) technology has recently been developed and helps to increase the accuracy of variant calling by minimizing polymerase chain reaction biases and sequencing errors. However, UMI-adopted targeted sequencing data analysis is slightly different from the methods for other types of omics data, and its pipeline for variant calling is still being optimized in various study groups for their own purposes. …”
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Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma by RUAN Zhao-wei, JIAO Xue-feng, XIAO Gao-tian, CHEN Jin-ling, LI Jun, LV Shu-ying

“…The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot. Finally, the effects of TMED2 knockdown and overexpression on the expression levels of TMED2, ADP-ribosylation factor 1, extracellular signal-regulated kinase ½, and phospho-extracellular signal-regulated kinase ½ proteins were examined in cells using the Western blot. …”
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CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance by Dijie Zheng, Yazhu Deng, Lu Deng, Zhiwei He, Xinghao Sun, Yanyu Gong, Binbin Shi, Deqin Lu, Chao Yu

“…Western blotting, immunohistochemistry, and real-time polymerase chain reaction were performed to detect CDCA7 expression in PC cells and tissues. …”
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Molecular diagnosis and epidemiological aspects of cutaneous Leishmaniasis in Aleppo: Current status by Lana Kourieh, Mohammad Y. Abajy, Mahasen Alkebajy, Silva Ishkhanian, Ream Nayal

“…This study aimed to evaluate the effectiveness of PCR (polymerase chain reaction) in diagnosing suspected CL cases. …”
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Determination of FMDV 146S particle concentration by spectrometric method during viral RNA quantification by M. I. Doronin, D. V. Mikhalishin, V. A. Starikov, D. A. Lozovoy, Yu. S. El’kina, A. V. Borisov

“…The spectrometric method showed 94.5–99.5% correlation with real time reverse transcription polymerase chain reaction and complement fixation test based on the results of tests of 360 non-inactivated suspensions of FMDV of all types. …”
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HMGB2 Deficiency Mitigates Abdominal Aortic Aneurysm by Suppressing Ang-II-Caused Ferroptosis and Inflammation via NF-κβ Pathway by Hao Wu, Legao Chen, Kaiping Lu, Yi Liu, Weiqin Lu, Jinsong Jiang, Chao Weng

“…Reverse transcription–quantitative polymerase chain reaction and western blot were utilized to assess HMGB2 levels. …”
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Network Pharmacology Analysis and Experimental Validation to Explore the Anti-inflammatory Mechanism of Asiatic Acid on Alcoholic Steatohepatitis by Tao Jiang, Jinhong Xu, Yang Lu, Xiaojin Chen, Yongxia Li

“…In addition, in the cellular model, real-time reverse transcriptase-polymerase chain reaction (Real-Time RT-PCR) and enzyme-linked immunosorbent assay (ELISA) results showed that AA could alleviate alcohol-induced cellular inflammation, while western blot and immunofluorescence results showed that AA might alleviate alcohol-induced cellular inflammation by inhibiting the nuclear factor-κB (NF-κB) pathway. …”
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