Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality by Mohammad Javad Zamani, Mohammad Reza Bihamta, Behnam Naserian Khiabani, Zahra Tahernezhad, Mohammad Taher Hallajian, Marzieh Varasteh Shamsi

“…Marker-assisted selection is the method which can recognize the alleles related to baking quality and this method is based on polymerase chain reaction. 10 pairs of specific primers related to Dx2, Dx2.1, Dx5, Dy10, and Dy12 subunits were used for recognizing baking quality of some wheat varieties and some mutant genotypes. …”
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Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese by Fengju Zhang, Tingzhun Zhu, Zhongjun Zhou, Yudong Wu, Yang Li

“…Genotyping was performed by direct sequencing after polymerase chain reaction(PCR) amplification and allele frequencies were tested for Hardy-Weinberg equilibrium. …”
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Epidemiology and Molecular Aspect of pediculosis among Primary School Children in Sulaimani Province Kurdistan-Iraq by Abdullah A. Haama

“…Direct contact, Sharing of clothing and combs can be a way for lice transmission from infected person to another.This study had been done to determine the pediculosis infection rate and risk factors asociassion with head lice among primary schools children in Sulaimani Province, all cases were evaluated by physical examination (visual inspection) and detail structured questionnaire which was including education levels, family size, hair washing frequency, type and length of hair, mother occupation and education, father occupation, and education was used, the polymerase chain reaction (PCR) used for confirming the head lice clade. …”
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Drug response is related to NR3C1 and FAAH polymorphism in Chinese pediatric epilepsy patients by Hongli Wang, Chu Li, Qian Li, Ning Li, Huiling Qin

“…Methods NR3C1 rs41423247 and FAAH rs324420 polymorphisms were detected by the polymerase chain reaction in 105 pediatric epilepsy patients. …”
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Markers of chronic hepatitis B at patients with HIV-infection and treatment approaches by S. I. Maksimov, S. P. Tsarenko, A. V. Kravchenko, N. Yu. Gankina, N. D. Yushchuk

“…All blood samples were investigated for the presence of НBV, HDV, HCV, HAV-virus markers in serum by enzyme-linked immunoassay and for the presence of RNA HIV, RNA HCV and DNA HBV by polymerase chain reaction.Results. It was revealed, that CHB markers are detected approximately at 5,5–6,8% of patients with HIV-infection, in most of the cases in combination to CHC or CHD markers. …”
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Endometriosis-Associated Massive Ascites in an Asian Woman: A Case Report of a Rare Clinical Entity by Nuntasiri Eamudomkarn, Naratassapol Likitdee, Pilaiwan Kleebkaow, Chumnan Kietpeerakool

“…Abdominal paracentesis was performed to yield fluid samples for evaluation, which revealed no malignant cells, and polymerase chain reaction (PCR) was negative for tuberculosis. …”
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Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis by Manar A. Abdul-Aziz, Amina K. Amin, Dalia H. El-Rouby, Olfat G. Shaker

“…The expression of VEGF-C was also assessed using immunohistochemistry and real-time polymerase chain reaction in 6 normal oral mucosa cases and 72 cases of OSCC. …”
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Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke by Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, Ya-guo Li

“…A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. …”
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Molecular Determination of Toxocara spp. Eggs Isolated from Public Parks and Playgrounds in Zahedan, Southeast Iran by Siavash Liravizadeh, Samaneh Abdolahi Khabisi, Alireza Salimi Khorashad, Hadi Mirahmadi

“…The modified Sheather’s flotation technique was employed to isolate Toxocara spp. eggs from the soil, followed by microscopic assessment and molecular evaluation of internal transcribed spacer 1 and 2 ribosomal deoxyribonucleic acid (ITS1 and 2 rDNA) using nested polymerase chain reaction (nested PCR) to identify the presence of Toxocara spp. eggs. …”
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The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran by Ali Taghipour, Majid Pirestani, Ramin Hamidi Farahani, Mohammad Barati

“…RNA extraction was performed, and then real time reverse-transcription polymerase chain reaction (rRT-PCR) assay was applied to detect viral RNA. …”
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Seroprevalence of Hepatitis C, Hepatitis B, Cytomegalovirus, and Human Immunodeficiency Viruses in Multitransfused Thalassemic Children in Upper Egypt by Ramadan A. Mahmoud, Abdel-Azeem M. El-Mazary, Ashraf Khodeary

“…It is therefore important to implement measures to improve blood transfusion screening, such as polymerase chain reaction, in order to reduce TTIs from blood donor units.…”
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Analysis of a Functional IL-6 Gene Polymorphism in HLAB27 Associated and Intermediate Uveitis Gives New Insight in Disease Pathogenesis and Commonality with Other Autoimmune Diseas... by Lindner Ewald, Langner-Wegscheider Beate, Sarny Stephanie, Renner Wilfried, El-Shabrawi Yosuf

“…Main outcome measures were genotype distribution and allelic frequencies determined by polymerase chain reaction. Results. The frequency of carriers of the minor allele for rs1800795 was significantly higher in patients with intermediate uveitis compared to controls (p=0.04; OR: 1.46; CI: 1.02–2.11). …”
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COVID-19 Outbreak among Healthcare Workers in a Specialty Unit in a Low-Middle Income Setting by Regina E. Oladokun, Babatunde O. Ogunbosi, Oreoluwa E. Morakinyo

“…Severe-acute-respiratory-syndrome-related coronavirus laboratory tests followed the World Health Organization recommendations, with nasopharyngeal swabs obtained for real-time reverse transcription–polymerase chain reaction. Results: Among the HCWs, a 49% COVID-19 positivity rate was found, with cases peaking in the third week. …”
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Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro by Sandra M. Ayuk, Nicolette N. Houreld, Heidi Abrahamse

“…Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of 84 genes in a PCR array. …”
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Promyelocytic Leukemia with No Retinoic Acid Receptor Alpha Abnormality but with RUNX1T1 Insertion to Chromosome 7q: A Classification and Management Dilemma by Kathleen Overholt, Terri L. Guinipero, Nyla A. Heerema, Michael R. Loken, Samir B. Kahwash

“…Bone marrow examination showed morphologic and immunophenotypic findings typical of APL, but conventional cytogenetics, fluorescence in situ hybridization (FISH), and real-time polymerase chain reaction (RT-PCR) showed no evidence of RARA rearrangements. …”
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Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India by Sonjoy Kumar Borthakur, Dilip Kumar Deka, Saidul Islam, Dilip Kumar Sarma, Prabhat Chandra Sarmah

“…Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. …”
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Expression and Secretion of CXCL-8 and CXCL-10 From <emph>Mycobacterium Bovis</emph> BCG-Infected Human Epithelial Cells: Role of IL-4

“…Gene expression of CXCL-8 and CXCL-10 was determined by the reverse transcription (RT)-polymerase chain reaction method. The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. …”
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Detection of Candida tropicalis from camel mastitis by S. A. Esmaeel, S. F. H. Al-Abedi, M. A. Altaliby, Q. T. Al-Obaidi

“…The present study was conducted to detect Candida tropicalis as a cause of camel mastitis (CM) us-ing routine diagnostic tests including Sabouraud’s dextrose agar (SDA) and API (analytical profile index) test strips, which were confirmed through amplification of the 18S rRNA gene of C. tropicalis using conventional polymerase chain reaction (c-PCR) technique and to investigate of the virulence genes of C. tropicalis including secreted aspartyl proteinase (SAPT4) and agglutinin-like sequence (ALST1) genes using c-PCR. …”

Homology Modeling and Expression of Recombinant NS5-RdRp Based on the Indonesian Local Strain of Dengue Virus for Anti-Dengue Drug Development by Toto Subroto, Ade Rizqi Ridwan Firdaus, Shinta Kusumawardani, Taufik Ramdani Tohari, Theresia Adventina Nababan, Wulan Pertiwi, Muhammad Fadhlillah, Sri Agung Fitri Kusuma, Yaya Rukayadi

“…We utilize reverse transcription- polymerase chain reaction (RT-PCR) and nested PCR for serotyping the DENV isolates, obtained from Hasan Sadikin Hospital, Indonesia. …”
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Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells by Wei Yu, Qing Li, Yuhua Yao, Yanping Quan, Yaozhou Zhang

“…Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction. Both genes were expressed in BmN cells using the Bac-to-Bac system. …”
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