Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1 by Shaofeng Xia, Chenliang Wang

“…The NSCLC cell lines H460 and A549 were employed in present work. hsa_circ_0003220, miR-489-3p, and insulin-like growth factors (IGF1) mRNA levels were assessed with a quantitative real time polymerase chain reaction (qRT-PCR). The cisplatin, docetaxel, and paclitaxel (PTX) resistances were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the enzyme linked immunosorbent assay (ELISA) test measured IGF1 expression. …”
Get full text

Effects of 0.01 mM strontium on human periodontal ligament stem cell osteogenic differentiation via the Wnt/-catenin signaling pathway by Tongke Sun, Dan Ma, Yang Song, Jing Hu, Ziqing Yang, Xu Wang, Jun Zhang

“…Quantitative reverse transcription polymerase chain reaction and western blots were used to examine the expression levels of relevant factors, such as collagen I (COL-1), ALP, and Runx family transcription factor 2 (RUNX2). …”
Get full text

Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs by Yubin Chen, Fen Liu, Fei Han, Lizhi Lv, Can-e Tang, Fanyan Luo

“…Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. …”
Get full text

Evaluation of miR-146a as a potential biomarker for diagnosis of cardiotoxicity induced by chemotherapy in patients with breast cancer by Nasrin Zare, Nasim Dana, Azam Mosayebi, Golnaz Vaseghi, Shaghayegh Haghjooy Javanmard

“…Materials and Methods: Using quantitative reverse transcription-polymerase chain reaction, the levels of miR-146a were assessed in the blood of 37 breast cancer patients receiving anthracyclines without cardiotoxicity and 33 breast cancer patients experiencing cardiotoxicity brought on by chemotherapy after chemotherapy. …”
Get full text

Molecular Detection of Minimal Residual Cancer in Surgical Margins of Head and Neck Cancer Patients by A. Peggy Graveland, Michiel de Maaker, Boudewijn J. M. Braakhuis, Remco de Bree, Simone E. J. Eerenstein, Elisabeth Bloemena, C. René Leemans, Ruud H. Brakenhoff

“…Here we investigated the suitability of quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) using human Ly-6D (hLy-6D) transcripts as molecular marker to detect MRC in surgical margins.…”
Get full text

Chronic Myeloid Leukemia Relapsing 25 Years after Allogenic Stem Cell Transplantation by Håkon Reikvam, Jørn Skavland, Stein-Erik Gullaksen, Randi Hovland, Tobias Gedde-Dahl, Øystein Bruserud, Bjørn Tore Gjertsen

“…We present a patient who relapsed 25 years after allo-SCT for chronic phase CML. Polymerase chain reaction (PCR) detected gradually evaluated levels of BCR-ABL1 transcripts, eventually leading to the diagnosis of relapsed disease. …”
Get full text

Prevalence of feline viral leukemia in Moscow and the Moscow Oblast with the analysis of hematological and biochemical blood parameters by A. P. Zenchenkova

“…Despite the statistical records that have been successfully kept in other countries for many years, there is little data on the prevalence of the infection in the Russian Federation, and the samples are represented by a small number of animals. 11,807 blood samples collected in Moscow and the Moscow Oblast were tested for FeLV antigen and antibodies against FeLV by polymerase chain reaction, enzyme immunoassay, and immunochromatographic assay. …”
Get full text

Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) by Roberta Lima Caldeira, Liana Konovaloffi Jannotti-Passos, Omar Dos Santos Carvalho

“…The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. …”
Get full text

Segmentasi Citra X-Ray Dada Menggunakan Metode Modifikasi Deeplabv3+ by Rima Tri Wahyuningrum, Maughfirotul Jannah, Budi Dwi Satoto, Amillia Kartika Sari, Anggraini Dwi Sensusiati

“…Reserve Transcription Polymerase Chain Reaction (RT-PCR) merupakan alat yang paling banyak digunakan untuk mendeteksi COVID-19. …”
Get full text

Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells by Yazi Huang, Beilei Zhao, Yahan Liu, Nanping Wang

“…In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPARγ increased the mRNA level of LPP1. …”
Get full text

The role of polymorphism (rs1800566) of NAD(F)H quinone oxidoreductase-1 in development of acute pancreatitis by T. A. Samgina, Y. V. Kanishchev, S. N. Grigor’Yev, P. M. Nazarenko, A. V. Polonikov

“…Genotyping of NQO1 gene polymorphism (rs1800566) was carried out by real-time polymerase chain reaction. Results. The association of polymorphism (rs1800566) of NQO1 gene T/T genotype with high risk of AP development, mostly - acute and non-biliary pancreatitis, in males. …”
Get full text

Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging by Md Sayed Ali Sheikh

“…Plasma miR-21 expression levels were determined by real-time polymerase chain reaction. To evaluate the diagnosis accuracy, the receiver operating characteristic (ROC) curves were used. …”
Get full text

Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis by Hua He, Tian Li

“…Analyses of NSCLC expression of hsa_circ_0000190, miR-1253, and interleukin 6 (IL-6) were conducted via a quantitative real-time polymerase chain reaction (qPCR) approach, while the ability of these tumor cells to resist DDP treatment was evaluated with a CCK-8 assay. …”
Get full text

A Case of Early Disseminated Neurological Lyme Disease Followed by Atypical Cutaneous Manifestations by Vamsi Kantamaneni, Vikas Sunder, Mohammad Bilal, Scott Vargo

“…The patient’s symptoms including facial droop resolved within 24 hours of antibiotic therapy. Polymerase chain reaction of the blood, IgM ELISA, and IgM Western blot testing for LD came back positive a few days after initiation of therapy. …”
Get full text

A Cytokine That Is Involved in Immune Responses in Chronic Hepatitis B Virus Patients by Noor R. Abady, Zeana Shakir Al-Al Hindi

“…Materials and Methods: There were four groups in the study: asymptomatic HBsAg carriers (n = 42), chronic hepatitis B (CHB, n = 57), liver cirrhosis (LC, n = 59), and the normal control group (n = 80). an Enzyme-Linked was used to measure the amounts of IL-17, while reverse transcription polymerase chain reaction was used to find IL-17 mRNA in peripheral blood mononuclear cells (PBMC). …”
Get full text

Polymorphisms of ATG5 Gene Are Associated with Autoimmune Thyroid Diseases, Especially Thyroid Eye Disease by Wen Wang, Zheng-yao Yu, Rong-hua Song, Shuang-tao He, Liang-feng Shi, Jin-an Zhang

“…Their frequencies in 824 AITD patients, including 271 HT patients and 553 GD patients, and 764 healthy controls were tested using both ligase detection reaction and multiplex polymerase chain reaction. Results. Allele A frequency of rs6568431 in AITDs patients (p=0.016, OR=1.201, 95% CI=1.034−1.394) and allele G frequency of rs6937876 in AITDs patients (p=0.009, OR=1.223, 95% CI=1.052−1.422) and in GD patients (p=0.009, OR=1.247, 95% CI=1.056−1.473) were significantly higher than those in the healthy controls. …”
Get full text

DNA MITOKONDRIA (mtDNA) SEBAGAI SALAH SATU PEMERIKSAAN ALTERNATIF UNTUK IDENTIFIKASI BAYI PADA KASUS INFANTISIDA by Taufik Hidayat

“…Perbandingan antara sampel DNA bayi dengan sampel DNA tersangka ibu menggunakan metode sekuensing PCR (Polymerase Chain Reaction). Pengambilan kesimpulan akhir pada pemeriksaan hubungan keibuan pada mtDNA harus dikombinasi dengan pemeriksaan forensik lainnya.…”
Get full text

Quantification and discovery of PCR inhibitors found in food matrices commonly associated with foodborne viruses by Cassandra Suther, Matthew D. Moore

“…Detection and quantification of norovirus commonly involves the use of reverse transcriptase quantitative polymerase chain reaction (RT-qPCR); however, the presence of inhibitory compounds in foods limit detection and accurate quantification. …”
Get full text

Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya by Ouko Abigael, Okoth Sheila, Amugune Nelson, Vesa Joutsjoki

“…Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay. The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein. …”
Get full text

A Canadian Isolate of Hepatitis D (delta) Virus by Véronique Poisson, Daniel B Ménard, Éric Frost, Jean-Pierre Perreault

“…The viral RNA from serum was amplified using reverse transcription coupled to polymerase chain reaction amplification. The resulting complementary DNA was cloned and sequenced. …”
Get full text