Investigation into the sero-molecular prevalence of Brucella melitensis in small ruminants in districts Mohmand and Charsadda Khyber Pakhtunkhwa Pakistan. by Sohaib Ul Hassan, Farhan Anwar Khan, Muhammad Shuaib, Muhammad Shahid, Said Sajjad Ali Shah, Shahrood Ahmed Siddiqui, Anthony Pokoo-Aikins, Ayman A Swelum

“…A total of 400 blood samples were collected from sheep and goats (n = 200 from each species) and analyzed by Rose Bengal precipitation test (RBPT), the indirect enzyme-linked immunosorbent assay (i-ELISA) and polymerase chain reaction (PCR). The findings of the study indicated 13.5% and 7% of sheep while 12.5% and 12.5% of goat's samples by RBPT and (i-ELISA) respectively. …”
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Evidence of High-Risk Human Papillomavirus in Esophageal Cancer in East Azerbaijan Province, Northwest of Iran by Zahra Sadeghian, Hossein Bannazadeh Baghi, Vahdat Poortahmasebi, Javid Sadeghi, Alka Hasani, Arezoo Azadi, Mahin Ahangar Oskouee

“…Methods. 140 paraffin-embedded specimens of esophageal tissues were investigated using nested-polymerase chain reaction (nested-PCR) with primer designing for the L1 region of HPV genome. …”
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ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway by Liang Jiang, Shuai Zhang, Ning An, Guoqing Chai, Changhong Ye

“…The data from two data sets (GSE76039 and GSE33630) were extracted and analyzed for the expression of ASPM, followed by a further validation in collected ATC patients using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The effect of ASPM on cell proliferation, migration, invasion, and cell cycle was explored in ATC cell lines by in vitro inhibition of ASPM, while ASPM-mediated tumorigenicity was investigated in a xenograft tumor model. …”
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Comparison of different methods for identification of meat species in meat products by Yusuf Özşensoy, Seyda Şahin

“…The identification of meat species in meat products was investigated using Agar Gel Immunodiffusion (AGID), and Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) methods.<p> <b>Results:</b> All of AGID, ELISA and PCR methods detected positivity for cattle meat in same samples. …”
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Determine the prevalence and fluoroquinolone resistance pattern of Escherichia coli ST131 among clinical isolates from vaginitis patients by Noor Ismeal Nasser, Mohammed Sabri Abdul-Razzaq, Nadia M. Al-Hili

“…Materials and Methods: A total of 150 high vaginal swabs were taken from female patients with aerobic vaginitis and were screened for the isolation and identification of E. coli ST131 using polymerase chain reaction technology that targets ST131-specific sequence polymorphisms in both the mdh and gyrB genes. …”
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COVID-19 and Pulmonary Tuberculosis Coinfection in a Moroccan Patient with Pulmonary Embolism: A Case Report and Literature Review by Imane Zouaki, Zakaria Chahbi, Mohamed Raiteb, Mohamed Zyani

“…We present a case of a sixty-seven-year-old male patient who was admitted to our COVID-19 emergency department with a diagnosis of COVID-19 pneumonia, confirmed by nasopharyngeal swab’s polymerase chain reaction (PCR) for detection of SARS-CoV-2. …”
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M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p by Shan Chen, Jian Liu, Lilei Zhu

“…Osteoclastogenesis was assessed using tartrate-resistant acid phosphatase staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The molecular mechanism was evaluated using microarray analysis, RT-qPCR, dual-luciferase reporter analysis, and RNA pull-down assay. …”
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Detection of Helicobacter pylori in City Water, Dental Units' Water, and Bottled Mineral Water in Isfahan, Iran by Ahmad Reza Bahrami, Ebrahim Rahimi, Hajieh Ghasemian Safaei

“…In the present study, totally 200 water samples were collected in Isfahan province and tested for H. pylori by cultural method and polymerase chain reaction (PCR) by the detection of the ureC (glmM) gene. …”
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CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway by Yu Ye, Yue Ke, Liu Liu, Tong Xiao, Jinhua Yu

“…The abilities of circFAT1 and miR-4781-3p in regulating PDLSC differentiation were analyzed by western blot, reverse transcription-polymerase chain reaction (RT-PCR), alkaline phosphatase (ALP), and Alizarin red staining (ARS). …”
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Acute Pancreatitis Complicating Acute Hepatitis E Virus Infection: A Case Report and Review by Hemanta Kumar Nayak, Nitish L. Kamble, Nishant Raizada, Sandeep Garg, Mradul Kumar Daga

“…The diagnosis of acute viral hepatitis E was confirmed by serology and reverse transcriptase polymerase chain reaction (RT-PCR) to demonstrate Hepatitis E virus (HEV) RNA in both stool and serum. …”
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Clinical Features and Severity of Leptospirosis Cases Reported in the Hawke’s Bay Region of New Zealand by Paul Sellors, Rebecca F. Watson, Rachel Bate, Gemma L. Bentham, Kathryn Haigh

“…Inclusion criteria were IgM positivity and diagnosis confirmed with either polymerase chain reaction (PCR) or microscopic agglutination test (MAT). …”
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Issues occult hepatitis B virus infection by I. A. Morozov, L. Yu. Ilchenko, N. I. Gromova, I. G. Fyedorov, I. V. Gordeychuk, A. K. Knyazhentseva, E. V. Zverkova, M. I. Mikhaylov

“…Molecular biologic enzymelinked immunoassay (ELISA), polymerase chain reaction (PCR) and morphological (immunohistochemistry, electron microscopy) methods were utilized for diagnostics.Results. …”
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A Case of Leptospirosis-Associated Severe Pulmonary Hemorrhagic Syndrome Successfully Treated with Venovenous Extracorporeal Membrane Oxygenation by Nao Umei, Shingo Ichiba

“…The leptospirosis diagnosis was confirmed by leptospiral DNA detection in urine samples by polymerase chain reaction and the results of paired serum antibody titer testing. …”
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Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection by José M. Alvarez, Ricardo J. Ordás

“…Evidence of the stable transgene integration was obtained by polymerase chain reaction for the nptII and uidA genes and expression of the uidA gene. …”
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Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata by Amira A. T. Al-Hosary, Jabbar Ahmed, Ann Nordengrahn, Malik Merza

“…As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. …”
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COVID-19 in Coincidence with Transient Distal Renal Tubular Acidosis in an Infant by Seyedeh-Kiana Razavi-Amoli, Hamid Mohammadjafari, Daniel Zamanfar, Mohammad Reza Navaeifar, Zahra Sadati-Lamradi, Mohammad Sadegh Rezai

“…The primary lab test showed leukocytosis, neutrophilia, elevated inflammatory markers, and non-anion-gap metabolic acidosis. Real-time polymerase chain reaction (RT-PCR) and elevated SARS-CoV-2 immunoglobulin M confirmed COVID-19, while echocardiography and spiral chest computed tomography scan were normal. …”
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Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe by Kudzai Fambisai, Petros Muchesa, Farisai Chidzwondo, Rumbidzai Mangoyi

“…Acanthamoeba castellannii species were isolated and confirmed present in Budiriro borehole water samples using non-nutrient agar, microscope, real-time Polymerase Chain Reaction (PCR), and gel electrophoresis. The selected plant samples, Murumanyama (Xeroderris stuhlmannii) bark extract, Munhundurwa (Solanum incanum) fruit extract and Mufandichimuka (Myrothamnus flabellifolius) stem and leaf extracts were then investigated for their anti-amoebic effects against the isolated Acanthamoeba castellannii, using agar well diffusion method. …”
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Analysis on the Susceptibility Genes in Two Chinese Pedigrees with Familial Parkinson's Disease by Changshui Xu, Jun Xu, Yanmin Zhang, Jianjun Ma, Hideshi Kawakami, Hirofumi Maruyama, Masaki Kamada

“…Fifty-one genomic DNA samples extracted from two Chinese pedigrees with FPD, the alpha-synuclein genes (SNCA), the leucine-rich repeat kinase 2(LRRK2), PINK1(PTEN-induced putative kinase 1), PARK7(Protein DJ1), PARK2(Parkinson juvenile disease protein 2), the glucocerebrosidase (GBA), and ATP(Ezrin-binding protein PACE-1), were sequenced by the use of polymerase chain reaction (PCR) technique. The gene dose of SNCA was checked. …”
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The Role of PCR in Diagnosis of a Rare Appendicular Tuberculosis and Mini Literature Review by Asmerom Tesfamariam Sengal, Ahmed Abdalla Mohamedani, Hanan Hasaan Hussein, Alaa Kamal

“…To confirm the diagnosis, molecular biology [polymerase chain reaction (PCR)] study was performed from the formalin fixed paraffin embedded (FFPE) appendicular tissue and revealed presence of Mycobacterium tuberculosis. …”
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Expression of protein phosphatase 4 in different tissues under hypoxia by Yanyan Ma, Jing Hou, Dengliang Huang, Yaogang Zhang, Zhe Liu, Meiyuan Tian

“…Quantitative polymerase chain reaction (qPCR) and western blot were used to detect the changes of protein phosphatase 4 catalytic subunit (PP4C) gene and protein in heart, lung, and brain tissues. …”
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