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201
Systematic Screening for SARS-CoV-2 to Detect Asymptomatic Infections: An Epitome of Taiwan’s Outbreak
Published 2022-01-01“…As care providers and gatekeepers of infectious diseases, Taipei City Hospital has to perform regular polymerase chain reaction (PCR) results of admitted patients and healthcare workers (HCWs) to achieve these goals. …”
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202
Flavonoids-rich extract of Aframomum melegueta (black pepper) improves antioxidant status and modulates aging process in lead-induced neurotoxic Drosophila melanogaster
Published 2024-06-01“…The expression and activities of the phase II antioxidant and age-related enzymes were determined using quantitative polymerase chain reaction (qPCR) to assess the oxidative and longevity effects of the extract. …”
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203
Clinical Significance of Molecular Diagnostic Tools for Bacterial Bloodstream Infections: A Systematic Review
Published 2016-01-01“…The majority of these tools focus on amplification based techniques such as polymerase chain reaction (PCR) which allows the detection of nucleic acids (both DNA and small RNAs) that are specific to bacterial species and sequencing or nucleic acid hybridization that allows the detection of bacteria in order to reduce delay of appropriate antibiotic therapy. …”
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204
Genetic Analysis of CYP2C9 with Reference to Drug Response in Epilepsy Patients of Pakistan
Published 2022-01-01“…Genomic DNA was isolated from blood, and amplification of rs1799853 (430C > T) and rs1057910 was carried out by polymerase chain reaction. Genotypes of CYP2C9 SNPs were determined by Sanger’s sequencing. …”
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205
Genetic Study In a Pakistani Family Reveals Autosomal Recessive Type of Artemis Deficiency
Published 2025-01-01“…Genetic analysis was performed on patients and available parents. Polymerase Chain Reaction (PCR) based amplification and electrophoresis in patients, parents and control was performed. …”
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206
Rare but Not so Rare? The Evolving Spectrum of Whipple's Disease
Published 2012-01-01“…Once considered the ideal case report, recent characterization of Tropheryma whippelii by means of broad range bacterial ribosomal DNA polymerase chain reaction (PCR) analysis (2,3) and its subsequent cultivation (4) has led to a veritable explosion of individual case reports, case series and hitherto unrecognized manifestations of the disease, such that it is now considered an underdiagnosed infectious disease (5). …”
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207
HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS
Published 2019-01-01“…No serological relatedness was identifed between the tested isolates and reference strain of serogroup A both using agglutination test (≤23.6%) and HI (≤12.2%). Polymerase chain reaction demonstrated that all the isolates recovered in the Russian Federation and Republic of Belarus in 2014-2016 belonged to serogroup B.…”
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208
Changes in Anterior Chamber Parameters Measured by Pentacam in Coronavirus Disease 2019 Infection: A Longitudinal Study
Published 2022-01-01“…All subjects exhibited a confirmed diagnosis of COVID-19 with a positive result of nasopharyngeal reverse transcription-polymerase chain reaction evaluation. Corneal Pentacam (Oculus, Dutenhofen, Germany) imaging was performed at least two weeks after recovery from systemic COVID-19. …”
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209
Brainstem and Cerebellar Involvement in Ramsay Hunt Syndrome
Published 2019-01-01“…Clinical awareness that Ramsay Hunt syndrome may also involve the brainstem and cerebellum is critical in evaluating the clinical neurologic findings and expanding the diagnostic workup to include brain magnetic resonance imaging and cerebrospinal fluid analysis, including varicella zoster polymerase chain reaction. Encephalitis requires longer duration administration of high-dose intravenous acyclovir in conjunction with steroids. …”
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210
Detection of Leishmania donovani DNA within Field-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) in Three Cutaneous Leishmaniasis Endemic Foci of Kurunegala District, Sri La...
Published 2021-01-01“…Field-caught sand flies were identified to the species level followed by DNA extraction. The polymerase chain reaction (PCR) was performed using species-specific primers to detect Leishmania donovani parasites. …”
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211
Antibiotic Susceptibility Profile of Aeromonas Species Isolated from Wastewater Treatment Plant
Published 2012-01-01“…Antibiotic susceptibility was determined using the disc diffusion method, and polymerase chain reaction (PCR) assay was employed for the detection of antibiotics resistance genes. …”
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212
Prevalence of Blood-Borne Viruses in Health Care Workers of a Northern District in Pakistan: Risk Factors and Preventive Behaviors
Published 2016-01-01“…Prevalence was investigated using serological assays followed by real time polymerase chain reaction (RT-PCR) based characterization. …”
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213
Herpes Simplex Virus Pneumonia: Importance of Aspiration Etiology
Published 2019-01-01“…HSV-1 infection was confirmed by immunostaining with monospecific monoclonal antibodies and by type-specific real-time polymerase chain reaction. It is very likely that HSV pneumonia was provoked by aspiration of infected exudates from the upper airway (namely, sequential infection from the tongue, epiglottis, and trachea to lung). …”
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214
Absence of the Genetic Marker IS6110 from a Strain of Mycobacterium tuberculosis Isolated in Ontario
Published 1998-01-01“…However, the insertion element IS6110, which is used for epidemiological tracing of M tuberculosis, was not detected in this strain by either restriction fragment length polymorphism analysis or by polymerase chain reaction. Two other genetic markers associated with the M tuberculosis complex, IS1081 and the direct repeat element, were present. …”
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215
Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models
Published 2020-01-01“…Histological staining techniques were used to verify the morphology and quantify the amount of lipid droplets present in the liver. Real-time polymerase chain reaction and immunoblotting were employed for monitoring protein expression and gene expression levels, respectively. …”
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216
Impacts of Horticultural Mineral Oils and Two Insecticide Practices on Population Fluctuation of Diaphorina citri and Spread of Huanglongbing in a Citrus Orchard in Sarawak
Published 2012-01-01“…Levels of HLB as determined by Polymerase Chain Reaction (PCR) were 42.2%, 9.4%, 11.4%, and 22.7%, respectively. …”
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217
Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe
Published 2024-09-01“…Acanthamoeba castellannii species were isolated and confirmed present in Budiriro borehole water samples using non-nutrient agar, microscope, real-time Polymerase Chain Reaction (PCR), and gel electrophoresis. The selected plant samples, Murumanyama (Xeroderris stuhlmannii) bark extract, Munhundurwa (Solanum incanum) fruit extract and Mufandichimuka (Myrothamnus flabellifolius) stem and leaf extracts were then investigated for their anti-amoebic effects against the isolated Acanthamoeba castellannii, using agar well diffusion method. …”
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218
Circulating LncRNA Is Potentially Up-regulated in Coronary Artery Disease
Published 2018-12-01“…The expression level was examined by semi-quantitative reverse transcriptase polymerase chain reaction technique. Our data shows that expression of HOTAIR is up-regulated in blood samples of patients with CAD.…”
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219
Cardiopulmonary Arrest and Pulmonary Hypertension in an Infant with Pertussis Case Report
Published 2021-01-01“…Bordetella pertussis infection was confirmed on respiratory polymerase chain reaction. She was treated with antibiotics, ventilatory management, and other supportive care. …”
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220
Association of Low Expression of NUMB in Peripheral Blood with Acute Myocardial Infarction
Published 2022-01-01“…The real-time quantitative polymerase chain reaction was used to measure the mRNA expression level of the NUMB gene in peripheral blood. …”
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