Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate by Lijuan Pei, Hongjie Zhang, Meihui Zhang, Yixuan Wang, Ke Wei

“…Rcor2, the corepressor 2 of REST, a transcriptional repressor, is predominantly expressed in embryonic stem cells (ESCs) and plays a major role in regulating ESC pluripotency and neurogenesis. The function of Rcor2 in development of other germ layers is yet unclear. …”
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Embryonic Stem Cell-Derived Cardiomyocyte Heterogeneity and the Isolation of Immature and Committed Cells for Cardiac Remodeling and Regeneration by Kenneth R. Boheler, Robert N. Joodi, Hui Qiao, Ondrej Juhasz, Amanda L. Urick, Sandra L. Chuppa, Rebekah L. Gundry, Robert P. Wersto, Rong Zhou

“…Pluripotent stem cells represent one promising source for cell replacement therapy in heart, but differentiating embryonic stem cell-derived cardiomyocytes (ESC-CMs) are highly heterogeneous and show a variety of maturation states. …”
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Culture and Use of Mesenchymal Stromal Cells in Phase I and II Clinical Trials by Bourin Philippe, Sensebé Luc, Planat-Bénard Valérie, Roncalli Jérôme, Bura-Rivière Alessandra, Casteilla Louis

“…Their potential has been extended to pluripotency, by their possibility of differentiating into tissues and cells of nonmesodermic origin. …”
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The Role of Epigenetic in Dental and Oral Regenerative Medicine by Different Types of Dental Stem Cells: A Comprehensive Overview by Ahmed Hussain, Hamid Tebyaniyan, Danial Khayatan

“…Several lines of evidence have shown that epigenetic modification of DNA and DNA-correlated histones are necessary for determining cells’ phenotypes and regulating stem cells’ pluripotency and renewal capacity. It is increasingly recognized that nuclear enzyme activities, such as histone deacetylases, can be used pharmacologically to induce stem cell differentiation and dedifferentiation. …”
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Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents by Foued Ghanjati, Simeon Santourlidis

“…Unrestricted somatic stem cells (USSCs) from human umbilical cord blood, which have a broad differentiation spectrum, reside in an uncommitted epigenetic state with partial methylation of the regulatory region of the gene coding for the pluripotency master regulator OCT4. Thus we hypothesized that further opening of this “poised” epigenetic state could broaden the differentiation potential of USSCs. …”
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Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts by Rangarajan Sambathkumar, Eric Kalo, Rob Van Rossom, Marijke M. Faas, Paul de Vos, Catherine M. Verfaillie

“…Interestingly, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts. …”
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An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro by Li Zhang, Yenan Wu, Xiang Li, Shao Wei, Yiming Xing, Zhengxing Lian, Hongbing Han

“…The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.…”
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Enigma of Retrotransposon Biology in Mammalian Early Embryos and Embryonic Stem Cells by Ying Yin, Liquan Zhou, Shuiqiao Yuan

“…Additionally, endeavors made to unveil the roles of these mysterious elements may facilitate stem cell status conversion and manipulation of pluripotency.…”
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Collagen Modulates the Biological Characteristics of WJ-MSCs in Basal and Osteoinduced Conditions by Vun Vun Hiew, Peik Lin Teoh

“…Gene expression profiles showed that COL not only maintained the pluripotency, self-renewal, and immunophenotype of WJ-MSCs but also primed cells toward lineage differentiations by upregulating BMP2 and TGFB1 genes. …”
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Mesenchymal Stem/Stromal Cells from Discarded Neonatal Sternal Tissue: In Vitro Characterization and Angiogenic Properties by Shuyun Wang, Lakshmi Mundada, Eric Colomb, Richard G. Ohye, Ming-Sing Si

“…Neonatal sternal bone MSCs (sbMSCs) were clonogenic, had a surface marker expression profile that was characteristic of bone marrow MSCs, were multipotent, and expressed pluripotency-related genes at low levels. Neonatal sbMSCs also demonstrated in vitro proangiogenic properties. …”
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TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6 by Sheng Luo, Wenjin Wang, Jingfang Feng, Rui Li

“…As a novel core part of pluripotency circuitry, testicular expression 10 (TEX10) plays an actively noticeable effect on reprogramming, early embryo development, and embryonic stem cell self-renewal. …”
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Long-Term Cultured Human Term Placenta-Derived Mesenchymal Stem Cells of Maternal Origin Displays Plasticity by Vikram Sabapathy, Saranya Ravi, Vivi Srivastava, Alok Srivastava, Sanjay Kumar

“…Flow analysis established bonafied MSCs phenotypic characteristics, staining positively for CD29, CD73, CD90, CD105 and negatively for CD14, CD34, CD45 markers. Pluripotency of the cultured MSCs was assessed by in vitro differentiation towards not only intralineage cells like adipocytes, osteocytes, chondrocytes, and myotubules cells but also translineage differentiated towards pancreatic progenitor cells, neural cells, and retinal cells displaying plasticity. …”
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Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis by Jurate Savickiene, Grazina Treigyte, Sandra Baronaite, Giedre Valiuliene, Algirdas Kaupinis, Mindaugas Valius, Audrone Arlauskiene, Ruta Navakauskiene

“…Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. …”
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Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro by Divya S. Varghese, Shama Parween, Mustafa T. Ardah, Bright Starling Emerald, Suraiya A. Ansari

“…In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers. However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade. …”
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The Impact of Bioactive Lipids on Cardiovascular Development by Alexander Kleger, Stefan Liebau, Qiong Lin, Götz von Wichert, Thomas Seufferlein

“…Interestingly, S1P may also act to maintain human stem cell pluripotency. Both LPA and S1P positively regulate the proliferative capacity of murine ES cells. …”
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Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production by Chloë Deelkens, Elly De Vlieghere, Mario Van Poucke, Masaki Kinoshita, Jeffrey Aalders, Lieven Thorrez, Bert Devriendt, Ann Van Soom, Luc Peelman, Björn Menten, Catharina De Schauwer, Jolanda van Hengel

“…These cells maintained core pluripotency markers (POU5F1, SOX2, SALL4, NANOG) and demonstrated the ability to differentiate into the three germ layers, indicating their potential for sustainable food applications. …”
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Migration, Proliferation, and Differentiation of Cord Blood Mesenchymal Stromal Cells Treated with Histone Deacetylase Inhibitor Valproic Acid by Leah A. Marquez-Curtis, Yuanyuan Qiu, April Xu, Anna Janowska-Wieczorek

“…We found that in MSC, VPA increased (i) the gene and total protein expression of CXCR4 and CXCR7 and primed migration towards a low gradient of SDF-1, (ii) the gene expression of MMP-2 and secretion and activation of proMMP-2, (iii) the proliferation and gene expression of pluripotency markers SOX2 and Oct-4, and exposure to lower concentrations of VPA (≤5 mM) had no effect on their differentiation to osteocytes and chondrocytes. …”
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Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis by Shiki Fujino, Norikatsu Miyoshi

“…The Oct4 gene plays an important role in undifferentiated embryonic stem cells and regulates stem cell pluripotency. The aim of this study was to examine the relationship between Oct4 expression and liver metastasis of colorectal cancer (CRC) in clinical samples and investigate the role and abilities of Oct4-positive CRC cells. …”
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Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore by Zhenkui Qin, Aichang Ji, Meng Yan, Danwen Liu, Xixi Li, Xiaoli Hu, Zhifeng Zhang

“…Immunofluorescence detection of KLF4, c-MYC, MYOSIN, and 5-HT indicated that CfT cells may possess a certain degree of pluripotency. Four gene transfer methods were tested in the CfT cell line; however, the transfer efficiency (0.5 %-1 %) requires further optimization. …”
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A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells by Hossein Khodadadi, Hiroaki Taniguchi

“…Validation of the differentiation was performed through quantitative reverse transcription PCR (RT-qPCR) analysis, measuring the expression levels of key neuronal markers, including Map2 and Pax6 along with the pluripotency marker Oct4. Additionally, the efficiency of the siRNA-mediated knockdown was confirmed by western blot analysis, which demonstrated significant gene silencing at protein levels. …”
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