Evaluation of Leea rubra Leaf Extract for Oxidative Damage Protection and Antitumor and Antimicrobial Potential by Nibedita Das, Sanowar Hossain, Jaytirmoy Barmon, Shahnaj Parvin, Mahadi Hasan, Masuma Akter, Ekramul Islam

“…EAF effectively inhibited AAPH-induced oxidative damage to DNA because it can inhibit the transformation of the supercoiled form of plasmid DNA to open circular and further linear form. …”
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Studies of the Antiproliferative Activity of Ruthenium (II) Cyclopentadienyl-Derived Complexes with Nitrogen Coordinated Ligands by Virtudes Moreno, Julia Lorenzo, Francesc X. Aviles, M. Helena Garcia, João P. Ribeiro, Tânia S. Morais, Pedro Florindo, M. Paula Robalo

“…Changes in the tertiary structure of pBR322 plasmid DNA were also observed in gel electrophoresis experiment and the images obtained by atomic force microscopy (AFM) suggest strong interaction with pBR322 plasmid DNA; the observed decreasing of the viscosity with time indicates that the complexes do not intercalate between DNA base pairs. …”
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Recombinant AAV batch profiling by nanopore sequencing elucidates product-related DNA impurities and vector genome length distribution by Florian Dunker-Seidler, Kathrin Breunig, Magdalena Haubner, Florian Sonntag, Markus Hörer, Rebecca C. Feiner

“…Our data highlight a high vector batch quality with low plasmid-derived and host cell DNA impurities of random origin, critical for mitigating associated safety risks. …”
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Emergence of a blaNDM-5-carrying extensively drug-resistant Enterobacter cloacae ST1718 in Saudi Arabia: Insights from comprehensive genome analysis by Ibrahim A. Al-Zahrani, Thamer M. Brek

“…The strain was found to harbor IncFIB(pECLA) and IncX3 plasmid replicons, with the latter encoding the blaNDM-5 gene. …”
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Mapping Antimicrobial Resistance in <i>Staphylococcus epidermidis</i> Isolates from Subclinical Mastitis in Danish Dairy Cows by Desiree Corvera Kløve, Mikael Lenz Strube, Peter M. H. Heegaard, Lærke Boye Astrup

“…Furthermore, the presence of plasmid replicon genes and the strain diversity among the <i>S. epidermidis</i> isolates was investigated to associate these findings with the observed AMR patterns. …”
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Production of a new variant of soluble trimer Env of HIV-1 CRF63_02A6 SOSIP.664 by Nadezhda B. Rudometova, Andrey P. Rudometov, Anastasia A. Fando, Danil I. Vahitov, Alexander A. Ilyichev, Alexander P. Agafonov, Larisa I. Karpenko

“…The designed gene was synthesized and cloned as part of an integration plasmid vector. A stable producer of trimer Env was obtained by transfection of the CHO-K1 cell line using the developed plasmid vector. …”
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Using genomics to explore the epidemiology of vancomycin resistance in a sewage system by Emilie Egholm Bruun Jensen, Saria Otani, Ivan Liachko, Benjamin Auch, Frank M. Aarestrup

“…The other end of these reads was mapped to generated metagenomic-assembled genomes and NCBI using BLAST. vanHBX could only be linked to the phylum level (Bacillota). Plasmid analysis of vanHBX Hi-C contigs showed that these were mainly located on plasmids reported found in enterococci species. …”
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Spontaneous Yersinia enterocolitica Septicemia in a Patient with Iron Overload by Steven S Larigakis, Michael A Noble, Terence G Sparling

“…The organism was biotype 1 serotype 0:5,27 and carried a virulence-encoding plasmid. It was calcium-dependent, autoagglutinating and virulent to orally challenged mice, but not resistant to the bacteriocidal activity of serum. …”
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RfxCas13d-mediated inhibition of Circ1647 alleviates renal fibrosis via PI3K/AKT signaling pathway by Yufang Ni, Hongmin Zhang, Qianwen Xian, Wenjie Qin, Hongwei Su, Li Wang, Jianchun Li

“…This study aimed to investigate the role of Circ1647 in renal fibrosis, which is a hallmark of CKD.Methods In this study, we established a unilateral ureteral obstruction (UUO) model and delivered Circ1647 RfxCas13d knockdown plasmid into renal parenchymal cells via retrograde injection through the ureter followed by electroporation. …”
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Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease by S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina

“…Site-directed plant genome modifcation by this technology involves the construction of Cas endonuclease- and guide-RNA-encoding vectors, delivery of the plasmid DNA into plant cells, processing of the chosen genomic target site by the corresponding gene products and regeneration of plants from modifed cells. …”
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Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatum by Peng Zhou, Bibek G C, Bo Hu, Chenggang Wu

“…We demonstrated that F. nucleatum cells expressing sacB in either a shuttle or suicide plasmid exhibit a lethal sensitivity to supplemental sucrose. …”
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DNA vaccines as promising immuno-therapeutics against cancer: a new insight by Alireza Shariati, Arya Khezrpour, Fatemeh Shariati, Hamed Afkhami, Hamed Afkhami, Hamed Afkhami, Aref Yarahmadi, Sajad Alavimanesh, Sajad Alavimanesh, Sina Kamrani, Mohammad Hossein Modarressi, Pouria Khani

“…Following the internalization of plasmids into cells, their genes are expressed and the tumor antigens are loaded on major histocompatibility molecules to be presented to T-cells. …”
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Deciphering the potential ability of DExD/H-box helicase 60 (DDX60) on the proliferation, diagnostic and prognostic biomarker in pancreatic cancer: a research based on silico, RNA-... by Dongdong Zhang, Enze Zhang, Ying Cai, Yixin sun, Peiji Zeng, Xiaohua Jiang, Yifan Lian

“…First, we constructed the plasmid and tested the plasmid effect by WB. Then MTT assay was performed to explore the effect of DDX60 knockout on the proliferation of pancreatic cancer cells. …”
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DNA Binding and Cleavage Activity of Binuclear Metal Complexes with Benzil-α-Monoxime Thiosemicarbzone by M. S. Surendra Babu, T. B. Patrudu, K. Hussain Reddy

“…The nuclease activity of these complexes has been investigated on double-stranded pBR322 circular plasmid DNA by using the gel electrophoresis experiments in presence and absence of oxidant (H2O2). …”
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Pathogenicity, Host Responses and Implications for Management of Enterohemorrhagic Escherichia coli O157:H7 Infection by Nathan K Ho, Aleah C Henry, Kathene Johnson-Henry, Philip M Sherman

“…The present review focuses on the history, epidemiology and evolution of the pathogen; provides a mechanistic overview of major virulence factors (including Shiga toxins, locus of enterocyte effacement pathogenicity island and pO157 plasmid); discusses host immune responses to infection; considers available animal models; and provides an overview of current and potential future management considerations.…”
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Biodegradation of Phenol at High Initial Concentration by <i>Rhodococcus opacus</i> 3D Strain: Biochemical and Genetic Aspects by Tatiana O. Anokhina, Tatiana Z. Esikova, Valentina N. Polivtseva, Nataliya E. Suzina, Inna P. Solyanikova

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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The resultant recombinant plasmid (pPICZα-A/PK-w) was transfected into K. phaffii K51 cells. …”
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Genetic profiling of multidrug-resistant Acinetobacter baumannii from a tertiary care center in Malaysia by Aisyah Syakirah Shahari, Navindra Kumari Palanisamy, Fadzilah Mohd Nor

“…The antibiotic resistance genes blaOXA-23, blaVIM, and blaADC were detected in both chromosomal and plasmid DNA. The ISAba1/blaOXA-23 gene was detected in 99.22% of the isolates. …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…Results. We developed a two-plasmid vector system to produce the recombinant FM1 antibody targeting the RSV F glycoprotein, using CHO cells as transient producers. …”
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DNA-loaded targeted nanoparticles as a safe platform to produce exogenous proteins in tumor B cells by Maria Cristina Grimaldi, Sara Bozzer, Dick J. Sjöström, Dick J. Sjöström, Linnea I. Andersson, Tom Eirik Mollnes, Tom Eirik Mollnes, Per H. Nilsson, Per H. Nilsson, Luca De Maso, Federico Riccardi, Michele Dal Bo, Daniele Sblattero, Paolo Macor

“…The targeting mechanism facilitated the interaction of NPs with target cells, thereby enhancing their internalization and subsequent exogenous plasmid DNA (pDNA) translation and protein expression. …”
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