Antibiotic Resistance of Salmonella enterica Serovar Typhi in Kolkata, India, and In Vitro Experiments on Effect of Combined Chemotherapy by Shyamapada Mandal, Manisha DebMandal, Nishith Kumar Pal

“…Among the isolates resistance to ampicillin (A), chloramphenicol (C), cotrimoxazole (Co) and tetracycline (T) were plasmid mediated; the plasmid was unstable in S. Typhi, and the other enteric bacteria like Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris were found to be the potential source of dissemination of such plasmids into S. …”
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Nuclear-Targeting Delivery of CRISPRa System for Upregulation of β-Defensin against Virus Infection by Dexamethasone and Phenylalanine Dual-Modified Dendrimer by Mingxiang Zuo, Xiaoxia Li, Shuang Liu, Bin Chen, Du Cheng

“…The dual-modified dendrimer containing dexamethasone (DET) and phenylalanine (Phe) was prepared to deliver plasmid DNA encoding dCas9 and single-guide RNA (sgRNA) for specific upregulation of β-defensin. …”
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The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer by Sirapat Settayanon, Pithi Chanvorachote, Apiwat Mutirangura

“…Recently, we used Box A of HMGB1 plasmid as molecular scissors to produce DNA gaps in normal cells. …”
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Homology Modeling and Expression of Recombinant NS5-RdRp Based on the Indonesian Local Strain of Dengue Virus for Anti-Dengue Drug Development by Toto Subroto, Ade Rizqi Ridwan Firdaus, Shinta Kusumawardani, Taufik Ramdani Tohari, Theresia Adventina Nababan, Wulan Pertiwi, Muhammad Fadhlillah, Sri Agung Fitri Kusuma, Yaya Rukayadi

“…The isolated NS5-RdRp gene from the local strain was then inserted into the pET28a(+) plasmid and transformed into E. coli BL21(DE3) as the host cell. …”
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Preliminary analysis of the role of small hepatitis B surface proteins mutations in the pathogenesis of occult hepatitis B infection via the endoplasmic reticulum stress-induced UP... by Huang Chengrong, Zhang Hao, Wang Jing, Li Jianfei, Liu Qian, Zong Qiyin, Zhang Yunyun, Wang Qin, Zhou Qiang

“…Cells transfected with OBI-related SHB mutation-encoding plasmids exhibited lower supernatant HBsAg levels than cells transfected with WT plasmids. …”
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Optimization of Conditions for Production of Soluble <i>E. coli</i> Poly(A)-Polymerase for Biotechnological Applications by Igor P. Oscorbin, Maria S. Kunova, Maxim L. Filipenko

“…Rosetta 2 (DE3) and Rosetta Blue (DE3) hosting the pRARE plasmid exhibited the lowest solubility, likely due to excessive translation efficiency. …”
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IS15DIV-flanked composite transposon harboring blaNDM-5 in multidrug-resistant Salmonella Typhimurium by Kaiting Zhao, Jing Jin, Yuan Liao, Aixia Liu, Wugao Liu, Weiping Wu

“…Besides, conjugation experiments showed that the transconjugant carried the incompatibility group I1 (IncI1)-I(alpha) plasmid replicon with the blaNDM-5 and sul1 genes, indicating that pLS20223695_NDM5 is a conjugative plasmid with transferability. …”
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Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag by Enos C. Kline, Rose Duong, Qin Wang, Barry R. Lutz

“…Key developments in PLP controls have enhanced the packing efficiency of RNAs into particles, reduced the complexity of their plasmid expression systems, and shifted purification from ultracentrifugation to affinity chromatography, producing progressively greater yields with higher purity. …”
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The Viral Load of Human Cytomegalovirus Infection in Children following Hematopoietic Stem Cell Transplant by Chip Digital PCR by Wen-Jun Wang, Miao Feng, Feng He, Juan Song, Qin-Qin Song, Dong Xia, Rong Liu, Hai-Lan Yao, Jun Han

“…To detect viral load in human cytomegalovirus (HCMV) infection children after hematopoietic stem cell transplant (HSCT) by chip digital PCR (cdPCR). Methods. The plasmid pUC57-UL83 containing the HCMV-UL83 gene and HCMV AD169 strain were used to evaluate the sensitivity of cdPCR. …”
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In Vitro Susceptibility and Florfenicol Resistance in Citrobacter Isolates and Whole-Genome Analysis of Multidrug-Resistant Citrobacter freundii by Wangxiao Zhou, Qianqian Chen, Changrui Qian, Kai Shen, Xinyi Zhu, Danying Zhou, Wei Lu, Zhewei Sun, Hongmao Liu, Kewei Li, Teng Xu, Qiyu Bao, Junwan Lu

“…Sequencing and comparative analysis of the plasmids from a multidrug-resistant C. freundii isolate named R47 showed that the floR-containing region in the plasmid pR47-54 was a truncated transposon-like structure and could be found on both plasmids and chromosomes of bacteria of either animal or human origin. …”
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Intricacies for Posttranslational Tumor-Targeted Cytokine Gene Therapy by Jeffry Cutrera, Denada Dibra, Arun Satelli, Xuexing Xia, Shulin Li

“…Electroporation-mediated transfection of cytokine plasmid DNA including a tumor-targeting peptide-encoding sequence is one method for obtaining a tumor-targeted cytokine produced by the tumor-bearing patient’s tissues. …”
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Second-Generation Triple Reporter for Bioluminescence, Micro–Positron Emission Tomography, and Fluorescence Imaging by Aparna H. Kesarwala, Julie L. Prior, Jinwu Sun, Scott E. Harpstrite, Vijay Sharma, David Piwnica-Worms

“…In living mice, somatic gene transfer of a ubiquitin promoter-driven FLuc-mNLS-SR39TK-IRES-MGFP plasmid showed a > 1,000-fold increase in liver photon flux and a > 2-fold increase in liver retention of 18 F-FHBG by microPET compared with mice treated with control plasmid. …”
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Complete genome sequence of Pseudarthrobacter sp. NIBRBAC000502770 from coal mine of Hongcheon on Republic of Korea by Min-Kyu Park, Yeong-Jun Park, Myung-Suk Kang, Min-Ha Kim, Soo-Young Kim, Jae-Ho Shin

“…The genome contains a chromosome (4,403,796 bp) and a plasmid (74,326 bp, named pMK-1) with 286-fold coverage. …”
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Antibiotics Resistance Pattern of Food-Borne Bacteria Isolated from Ice Cream in Bangladesh: A Multidisciplinary Study by Md Sohel, Moushumi Akter, Md. Fahmid Hasan, Shahin Mahmud, Mohammod Johirul Islam, Ashekul Islam, Khairul Islam, Abdullah Al Mamun

“…All individual isolates were screened for plasmid DNA, and we found that seven strains harbored a single or more than two plasmids sized approximately between 1.9 and 140 MDa, indicating a possible connection between resistance phenotype pattern and genotype.…”
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Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans by W. Buwembo, S. Aery, C. M. Rwenyonyi, G. Swedberg, F. Kironde

“…A set of recombinant plasmids carrying different point mutations in cloned folP were separately transformed into folP-deficient Escherichia coli. …”
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Establishment of a rapid method for the detection of Brucella canis based on recombinase-mediated thermostable nucleic acid amplification technology by Shao-Zheng Song, Zi-Yuan Li, Yuan-Yuan Liu, Ying-Chao Wu, Kang-Ying Yu, Zhengyi He

“…Primers and fluorescent probes were designed and synthesized, and recombinant plasmids were constructed as standards. The RAA assay was optimized by screening primers and establishing a fluorescent reaction system. …”
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Genome Characteristic of NDM-5-Producing Klebsiella Quasipneumoniae and Klebsiella Pneumoniae in China by Chen L, Liu R, Ye H, Qian J, Lou D

“…Both strains exhibited multidrug resistance, including reduced susceptibility to carbapenems, and carried transferable NDM-5-bearing plasmids. Specifically, S1-pulsed-field gel electrophoresis (PFGE), southern blotting, and whole-genome sequencing revealed that ACESH02628 contained an IncHI2/IncHI2A-type plasmid, while ACESH02857 carried an IncX3-type plasmid, both associated with the regional spread of NDM-5. …”
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Patterns of Antibiotic Resistance in Enterobacteriaceae Isolates from Broiler Chicken in the West Region of Cameroon: A Cross-Sectional Study by Jude Fonbah Leinyuy, Innocent Mbulli Ali, Ousenu Karimo, Christopher B. Tume

“…Antibiotic susceptibility was tested on Mueller-Hinton-Agar by disc diffusion. Plasmid-borne genes for extended-spectrum beta lactamase (ESBL) and resistance to Quinolones (PMQR) and Aminoglycosides were detected by standard endpoint polymerase chain reaction (PCR). …”
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The effect of thioredoxin and prochymosin coexpression on the refolding of recombinant alpaca chymosin by S. V. Belenkaya, D. N. Shcherbakov, A. I. Chapoval, T. I. Esina, V. V. Elchaninov

“…It is shown that Escherichia coli strain BL21 transformed with the pET-TrxProChn plasmid provides an efficient synthesis of the thioredoxin-prochymosin chimeric molecule. …”
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Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower by Jude E. Maul, John Lydon, Dilip Lakshman, Colin Willard, Hyesuk Kong, Daniel P. Roberts

“…The pTagA-like plasmid contained a complete Type IV secretion system (T4SS) with associated putative killer protein. …”
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