Recent advances in recombinant production of soluble proteins in E. coli by Ario de Marco

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Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 by Xiaofang Xie, Xiaofang Xie, Yaxuan Zhai, Zhichen Zhu, Feinan Qian, Feinan Qian, Jie Zhu, Qizhao Gao, Hong Du, Hong Du

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Resistome phylodynamics of multidrug-resistant Shigella isolated from diarrheal patients by Asaduzzaman Asad, Md. Abu Jaher Nayeem, Md. Golam Mostafa, Ruma Begum, Shah Nayeem Faruque, Suraia Nusrin, Israt Jahan, Shoma Hayat, Zhahirul Islam

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Electroporation of mouse embryonic stem cells with Neon device by I. P. Savchenkova, A. A. Savchenkova

Subjects: “…insertion of exogenous dna plasmid…”
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First Detection and Genomic Characterization of Linezolid-Resistant <i>Enterococcus faecalis</i> Clinical Isolates in Bulgaria by Tanya V. Strateva, Preslava Hristova, Temenuga J. Stoeva, Hristina Hitkova, Slavil Peykov

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Plasmidome of Salmonella enterica serovar Infantis recovered from surface waters in a major agricultural region for leafy greens in California. by Beatriz Quiñones, Bertram G Lee, Ashley Avilés Noriega, Lisa Gorski

“…Plasmid nucleotide queries did not identify the emerging megaplasmid pESI in the examined Infantis strains; however, the detected plasmids each had similarity to a plasmid sequence already cataloged in the nucleotide databases. …”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The newly constructed plasmid was then transformed into the outbreak and non-outbreak strains of S. …”
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Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation by Simonetta Geninatti Crich, Stefania Lanzardo, Alessandro Barge, Giovanna Esposito, Lorenzo Tei, Guido Forni, Silvio Aime

“…Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. …”
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Enhancing DNA Vaccine Delivery Through Stearyl-Modified Cell-Penetrating Peptides: Improved Antigen Expression and Immune Response In Vitro and In Vivo by Sheng Jiang, Cheng Zu, Bin Wang, Yiwei Zhong

“…High-content imaging revealed that the S-Cr9T–plasmid complex stably adhered to the cell membrane, leading to enhanced plasmid uptake and transfection. …”
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Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria by donya kazemi, abbas doosti, mostafa Shakhsi niaee

“…The recombinant plasmid was extracted from transformed bacteria. L. lactis transformed by electroporation both with the recombinant plasmid containing the target gene pNZ8148-Usp45-BAB1-0278 and the plasmid without the target gene. …”
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STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES

“…It was found that 75% of the Gram negative and 58% Gram positive clinical isolates have plasmid and probably the higher resistance pattern was due to presence of R-plasmid. …”
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CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

“…To deal with this obstacle, we have constructed a finetuned, inducible two-plasmid CRISPR/Cas9-system for chromosomal gene insertion in L. plantarum. …”
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A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran by Sahar Samsami, Sahar Namavari, Saeed Ataei, Abdolmajid Ghasemian, Ava Yazdanpanah, Neda Sepahi, Gholamreza Hatam, Hossein Faramarzi, Hadi Mirzaei, Razie Ranjbar, Ali Ghanbariasad

“…Nested PCR exhibited a lower detection limit for L. infantum of 4 × 106 plasmid copy number compared to multiplex LAMP and RT-qPCR. …”
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Construction and Antiapoptosis Activities of Recombinant Adenoviral Expression Vector Carrying EBV Latent Membrane Protein 2A by Xishuang Liu, Yu Gao, Bing Luo, Youan Zhao

“…To evaluate the possible effects of LMP2A (EBV latent membrane protein 2A) on human gastric cancer cell line SGC-7901, LMP2A coding gene was subcloned into shuttle plasmid pAdTrackCMV to form transfer plasmid pAdTrackCMV-2A, which was linearized with PmeI and cotransformed into E.coli BJ5183 with adenovirus genomic plasmid of pAdeasy-1. …”
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Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia by Vengadesh Letchumanan, Nurul-Syakima Ab Mutalib, Sunny Hei Wong, Kok-Gan Chan, Learn-Han Lee

“…Only 338 V. parahaemolyticus isolates had 1-7 different plasmids and could be categorized into 27 patterns based on the number and pattern of plasmid present. …”
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Cryo-EM structure and evolutionary history of the conjugation surface exclusion protein TraT by Chloe Seddon, Sophia David, Joshua L. C. Wong, Naito Ishimoto, Shan He, Jonathan Bradshaw, Wen Wen Low, Gad Frankel, Konstantinos Beis

“…Following transfer of IncF-like plasmids, recipients become refractory to a second wave of conjugation with the same plasmid via entry (TraS) and surface (TraT) exclusion mechanisms. …”
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SNX30 inhibits lung adenocarcinoma cell proliferation and induces cell ferroptosis through regulating SETDB1 by Xinjie Fan, Qichu Zhu, Chengzhuo Du, Jinlai Chen, Yingming Su

“…We observed that the level of SETDB1 was lower in the SNX30-plasmid group than in the control-plasmid group, whereas the opposite results in ferrostatin-1 treated cells. …”
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Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation by Shozo Sonoda, Katsuro Tachibana, Toshifumi Yamashita, Makoto Shirasawa, Hiroto Terasaki, Eisuke Uchino, Ryo Suzuki, Kazuo Maruyama, Taiji Sakamoto

“…This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. …”
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Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia by Vurmila Venggadasamy, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Hooi-Leng Ser, Vengadesh Letchumanan, Priyia Pusparajah

“…The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. …”
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Molecular Epidemiology of Escherichia coli Resistant to Carbapenems, Fluoroquinolones, and Aminoglycosides Isolated from One of the Largest Hospitals in Vietnam in 2014–2019 by Tohru Miyoshi-Akiyama, Do Van Thanh, Truong Thai Phuong, Nguyen Quang Huy, Pham Thi Phuong Thuy, Teruo Kirikae, Pham Hong Nhung, Norio Ohmagari

“…Dominant plasmid type carrying drug resistance gene was IncQ1_1. …”
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