A novel two-component system contributing the catabolism of c-di-GMP influences virulence in Aeromonas veronii by Chaolun Liu, Jia Shao, Xiang Ma, Yanqiong Tang, Juanjuan Li, Hong Li, Xue Chi, Zhu Liu

“…To evaluate the impact of ArrS/ArrR on intracellular c-di-GMP levels, biofilm detection, motility detection, fluorescence reporter plasmids, and LC-MS/MS analysis were employed. One-hybrid, EMSA, and RT-qPCR were used to demonstrate the function of ArgR on arrSR promoter. …”
Get full text

Restoration of Genetic Code in Macular Mouse Fibroblasts via APOBEC1-Mediated RNA Editing by Sonali Bhakta, Hiroko Kodama, Masakazu Mimaki, Toshifumi Tsukahara

“…Menkes disease is a recessive X-chromosome-linked hereditary syndrome in humans, caused by defective copper metabolism due to mutations in the <i>ATP7A</i> gene, which encodes a copper transport protein. Here, we generated plasmids encoding the MS2 system and the APOBEC1 deaminase domain and used a guide RNA with flanking MS2 sites to restore mutated <i>Atp7a</i> in fibroblasts from a macular mouse model of Menkes disease withs T>C mutation. …”
Get full text

Paeoniflorin alleviates high glucose-induced endothelial cell apoptosis in diabetes mellitus by inhibiting HRAS-activated RAS pathway by Wenting Yu, Hongchun Jiang

“…Human umbilical vein endothelial cells (HUVECs) were separately treated with HG, Pae (5, 10, 20 μM) and/or HRAS overexpression plasmids (oe-HRAS). The cell viability, apoptosis and the protein expressions of HRAS and Ras-GTP were evaluated. …”
Get full text

Use of a Sendai virus-based vector for effcient transduction of pinniped fbroblasts by V. R. Beklemisheva, A. G. Menzorov

“…There are several ways to deliver reprogramming transcription factors: RNA, proteins, plasmids, viral vectors etc. The most effective delivery systems for mouse and human cells are based on viral vectors. …”
Get full text

Extracellular vesicle surface engineering with integrins (ITGAL & ITGB2) to specifically target ICAM-1-expressing endothelial cells by Markus Bergqvist, Kyong-Su Park, Nasibeh Karimi, Lijuan Yu, Cecilia Lässer, Jan Lötvall

“…To target these cells, we overexpress the ICAM-1 binding receptor “lymphocyte function-associated antigen-1” (LFA-1) in HEK293F cells, by sequential transfection of plasmids of the two LFA-1 subunits, ITGAL and ITGB2 (CD11a and CD18). …”
Get full text

GENOME POLITICS: STRATEGY AND TACTICS by Jonas Rubikas

“…This is clearly seen when the comparison between the information content (the gene number) and the life cycle of small genomes (plasmids, viruses, singlecellular prokaryotic organisms) is made: large space is occupied by information on replication enzymes and the sequence of replication origin. …”
Get full text

Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis by Parsa Kamali, Gregory Fairn

“…Experimentally, the system introduces two plasmids: one carrying the Sleeping Beauty transposase and the other with an integration cassette carrying the gene of interest, a reverse-doxycycline controlled repressor gene, and an antibiotic resistance gene. …”
Get full text

Deep-Sea Ecosystems as an Unexpected Source of Antibiotic Resistance Genes by Wei Zhang, Yingdong Li, Yunmeng Chu, Hao Liu, Hongmei Jing, Qianfeng Xia

“…Mobile genetic elements, such as IncQ and RP4 plasmids, were also identified. The ratio of nonsynonymous to synonymous substitutions (pN/pS) values of these genes suggest different evolutionary strategies in response to deep-sea conditions and possible human impacts. …”
Get full text

Oxymatrine inhibits the development of radioresistance in NSCLC cells by reversing EMT through the DcR3/AKT/GSK-3β pathway by Jianming Tang, Yu Cao, Hong Zhang, Rui Wang

“…Invasion and migration by NSCLC cells were quantified using Transwell and wound healing assays. Plasmids were used to overexpress decoy receptor 3 (DcR3) in A549 cells. …”
Get full text

Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression by Michela Cioni, Christian Mittelholzer, Marion Wernli, Hans H. Hirsch

“…Methods UTA-6 or primary human renal proximal tubular epithelial cells (RPTEC) were co-transfected with plasmids constitutively expressing agnoprotein, or ICP47, and enhanced green-fluorescent protein (EGFP). …”
Get full text

The Formation and Features of Massive Vacuole Induced by Nutrient Deficiency in Human Embryonic Kidney Cells by Dakang Sun, Xinye An, Yanli Cheng

“…Methods: LysoTracker Deep Red probes and Enhanced Green Fluorescent Protein-tagged light chain 3B (LC3B) plasmids were employed to differentiate the types of massive vacuoles. …”
Get full text

Host-pathogen interaction in invasive Salmonellosis. by Hanna K de Jong, Chris M Parry, Tom van der Poll, W Joost Wiersinga

“…Genes for these factors are commonly carried on Salmonella Pathogenicity Islands (SPIs). Plasmids also carry putative virulence-associated genes as well as those responsible for antimicrobial resistance. …”
Get full text

Adaptive modification of antiviral defense systems in microbial community under Cr-induced stress by Dan Huang, Jingqiu Liao, Jose Luis Balcazar, Mao Ye, Ruonan Wu, Dongsheng Wang, Pedro J. J. Alvarez, Pingfeng Yu

“…Furthermore, the proportion of antiviral defense systems carried by mobile genetic elements (MGEs), including plasmids and viruses, increased by approximately 43% and 39%, respectively, with rising Cr concentrations. …”
Get full text

Isolation and Characterization of Colistin-Resistant <i>Enterobacteriaceae</i> from Foods in Two Italian Regions in the South of Italy by Rosa Fraccalvieri, Angelica Bianco, Laura Maria Difato, Loredana Capozzi, Laura Del Sambro, Stefano Castellana, Adelia Donatiello, Luigina Serrecchia, Lorenzo Pace, Donatella Farina, Domenico Galante, Marta Caruso, Maria Tempesta, Antonio Parisi

“…Additionally, in four of them were predicted the IncHI2 plasmids, known to facilitate the spread of colistin resistance. …”
Get full text

Grem1 inhibits osteogenic differentiation of MBMSCs in OVX rats through BMP/Smad1/5 signaling pathway by Shan-shan Yang, Min LinHu, Xiao-hua Hu, Si-si Jiang, Wen-yue Hu, Xiao-hong Yang

“…Techniques such as Western Blot (WB) and Real-Time Quantitative PCR (RT-qPCR) were employed to determine changes in Grem1 expression in MBMSCs of postmenopausal rats and its effect on osteogenic differentiation. Plasmids for Grem1 overexpression and siRNA for Grem1 knockdown were transfected into MBMSCs, and WB was used to assess the regulatory role of Grem1 on MBMSCs osteogenic differentiation. …”
Get full text

Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity by Thunchanok Yaikhan, Kamonnut Singkhamanan, Pawarisa Luenglusontigit, Arnon Chukamnerd, Natakorn Nokchan, Nutwadee Chintakovid, Sarunyou Chusri, Rattanaruji Pomwised, Monwadee Wonglapsuwan, Kittinun Leetanaporn, Surasak Sangkhathat, Komwit Surachat

“…In addition to the AMR complexity, we found that three isolates carried the bla NDM gene on IncN2 plasmids, further emphasizing the urgency of monitoring and managing ECC-related infections. …”
Get full text

Prevalence and genomic insights of carbapenem resistant and ESBL producing Multidrug resistant Escherichia coli in urinary tract infections by Vidhyalakshmi Sivarajan, Amirtha Varshini Ganesh, Pavithra Subramani, Priyanka Ganesapandi, R. N. Sivanandan, Sneha Prakash, Nithyasri Manikandan, Arunasalam Dharmarajan, Frank Arfuso, Sudha Warrier, Marquess Raj, Kumar Perumal

“…Virulence factors of CREC 3 (fliC, shuA), CREC 4 (spaS), CREC 5 (iucA, papH, papG, iucB, yigF), and plasmids (IncFIA, IncFIB) were identified to be significant. …”
Get full text

Identification of the Bovine <i>CSN3</i> Core Promoter Region and the Relationships Between <i>CSN3</i> Promoter Polymorphisms and the <i>CSN3</i> A and B Alleles by Wenqing Li, Xiaoyang Wang, Xiuyang Xu, Pinhui Wu, Tong Fu, Liyang Zhang, Tengyun Gao

“…Therefore, a series of 5′-deleted promoter plasmids were constructed to define the core promoter of <i>CSN3</i>. …”
Get full text

From Traditional Typing to Genomic Precision: Whole-Genome Sequencing of <i>Listeria monocytogenes</i> Isolated from Refrigerated Foods in Chile by Julio Parra-Flores, Beatriz Daza-Prieto, Pamela Chavarria, Miriam Troncoso, Anna Stöger, Guillermo Figueroa, Jetsi Mancilla-Rojano, Ariadnna Cruz-Córdova, Aleksandra Martinovic, Werner Ruppitsch

“…The pCFSAN010068_01 plasmids were prevalent, and insertion sequences (ISLs) and composite transposons (cns) were detected in 87.5% of the strains. …”
Get full text

Identification of the RHD novel allele c. 801+2T>G and study of its effect on RhD phenotype in vitro by JIA Shuangshuang, WEN Jizhi, WEI Ling, ZHANG Runqing, LUO Guangping, JI Yanli

“…Sanger sequencing was used to analyze the sequence of RHD genes and the newly identified splicing site mutations of RHD genes were used to construct pSplicePOLR2G micro gene expression plasmids. By using an in vitro micro gene splicing system, the mRNA splicing results were detected and analyzed using agarose and capillary electrophoresis to predict their impact on RhD phenotype. …”
Get full text