Variant rs4149584 (R92Q) of the TNFRSF1A gene in patients with familial multiple sclerosis by U. Gomez-Pinedo, J.A. Matías-Guiu, L. Torre-Fuentes, P. Montero-Escribano, L. Hernández-Lorenzo, V. Pytel, P. Maietta, S. Alvarez, I. Sanclemente-Alamán, L. Moreno-Jimenez, D. Ojeda-Hernandez, N. Villar-Gómez, M.S. Benito-Martin, B. Selma-Calvo, L. Vidorreta-Ballesteros, R. Madrid, J. Matías-Guiu

“…We also conducted a functional study to analyze the pathogenic mechanism of variant rs4149584 (-g.6442643C > G, NM_001065.4:c.362 G > A, R92Q) by plasmid transfection into human oligodendroglioma (HOG) cells, which behave like oligodendrocyte lineage cells; protein labeling was used to locate the protein within cells. …”
Get full text

Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps) by Timothy R. Ganderton, Daniel Ghete, Karen Hogg, Graeme J. Park, Christoph G. Baumann, Anthony J. Wilkinson, Paul R. Pryor

“…Numerous vap genes are found on virulence plasmids isolated from different species, and the Vaps share a high degree of sequence identity. …”
Get full text

A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods by Daniela Hübscher, Sabine Rebs, Luis Haupt, Thomas Borchert, Celina Isabell Guessoum, Franziska Treu, Steffen Köhne, Andreas Maus, Mario Hambrecht, Samuel Sossalla, Ralf Dressel, Angela Uy, Mark Jakob, Gerd Hasenfuss, Katrin Streckfuss-Bömeke

“…Reprogramming efficiencies were in accordance with the literature, indicating that the parental cell type and the reprogramming method play a major role for the reprogramming efficiencies (FB: STEMCCA: 1.30±0.18, Sendai virus: 1.37±0.01, and episomal plasmids: 0.04±0.02; PBMCs: Sendai virus: 0.002±0.001, episomal plasmids: 0) but result in the same characteristics of pluripotency. …”
Get full text

Exploiting CRISPR/Cas system in controlling antibiotic resistance of bacteria by Pourya Gholizadeh, Elnaz Faghfuri, Khudaverdi Ganbarov, Hossein Samadi Kafil

“…Antimicrobial resistance genes are mainly transmitted through horizontal gene transfer (HGT) mediated by transposable elements, phages, and plasmids, which leads to the emergence of multidrug-resistant bacteria. …”
Get full text

Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories by Thibaut Vedani, Matthieu Pot, Thomas Garrigos, Loïk Sababadichetty, Marion Daniel, David Wilkinson, Thierry Benoit-Cattin, Olivier Belmonte, Patrick Mavingui, Laurent Dortet, Guillaume Miltgen

“…Finally, all isolates (100%) carried the bla NDM-5 or bla OXA-181 genes on plasmids (IncF2, IncX3), most of which were conserved and identified in various STs. …”
Get full text

A functional SNP of the core promoter region within goat CDC25A gene affects litter size by Taiyuan Zhang, Jingxuan Wang, Yangyang Bai, Qian Wang, Ke Wang, Haijing Zhu, Lei Qu, Zhengang Guo, Chuanying Pan, Xianyong Lan

“…The results displayed that the higher relative luciferase activity was observed in plasmids carrying the A allele rather than the C allele. …”
Get full text

Recombinant Lactococcus lactis Carrying IL-4 and IL-10 Coding Vectors Protects against Type 1 Diabetes in NOD Mice and Attenuates Insulitis in the STZ-Induced Model by Tatiane M. Preisser, Vanessa P. da Cunha, Mariana P. Santana, Vanessa B. Pereira, Denise C. Cara, Bianca M. Souza, Anderson Miyoshi

“…Therefore, the aim of this study was to investigate the effect of orally administered Lactococcus lactis MG1363 FnBPA+ strains carrying plasmids encoding IL-4 and IL-10 in the streptozotocin- (STZ-) induced diabetes model and in nonobese diabetic (NOD) mice. …”
Get full text

‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. …”
Get full text

Production and characterization of nervous necrosis virus vaccine antigens in wild type and CRISPR/Cas9 genome edited Nicotiana benthamiana and edible crop lettuce Lactuca sativa by Iva Andrasevic, Hang Su, Espen Rimstad, Jihong Liu Clarke

“…In the present study, we report: (1) selection and design of recombinant plasmids encoding the capsid proteins (CPs) of two genotypes of NNV, red-spotted grouper nervous necrosis virus (RGNNV) and striped jack nervous necrosis virus (SJNNV); (2) design and optimization of plasmids for transient expression of NNV vaccine antigens in wild type Nicotiana benthamiana, CRISPR/Cas9 genome edited Nicotiana benthamiana and Lactuca sativa; (3) test of different Agrobacterium strains (LBA4404 and AGL1) for effective production of NNVCPs; (4) the expression patterns of NNVCPs over time post infiltration for different plants and cultivars; (5) successful production of NNV antigens in N. benthamiana lines and lettuce cultivars, indicating the potential of the plants as antigen producers in the development of a plant-based vaccine against VNN.…”
Get full text

Toxin and capsule production by Bacillus cereus biovar anthracis influence pathogenicity in macrophages and animal models. by Treenate Jiranantasak, Andrew P Bluhm, Donald J Chabot, Arthur Friedlander, Richard Bowen, Ian A McMillan, Ted L Hadfield, Airn Hartwig, Jason K Blackburn, Michael H Norris

“…Genomic analyses revealed Bcbva as a member of the B. cereus species that carries two plasmids, pBCXO1 and pBCXO2, which have high sequence homology to the B. anthracis toxin and polyglutamate capsule encoding plasmids pXO1 and pXO2, respectively. …”
Get full text

Priestia flexa as a Novel Urinary Tract Pathogen in Daloa, Côte d’Ivoire: Insights From Genomic Sequencing by Dého Aristide Gbégbé, Okran Beyosse Christophe Kacou, N’goran Parfait N’zi, Djédoux Maxime Angaman

“…Strain 21LM07 was characterised by the absence of plasmids in its genome. Two plasmids were predicted in the genomes of isolates 21LM367 and 21LM1136; however, rep7a and IncI2 were predicted to contain the tet(K) resistance gene. …”
Get full text

High-quality draft genome and characterization of commercially potent probiotic strains by Ayesha Sulthana, Suvarna G. Lakshmi, Ratna Sudha Madempudi

“…The genomic analysis of all the strains confirmed the absence of genes encoding putative virulence factors, antibiotic resistance, and the plasmids.…”
Get full text

Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli by Tianzhen Xiong, Xinmeng Li, Wei Liu, Huidie Yue, Junling Liu, Dingyuan Bai, Wei Li, Guangyan Fan

“…We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production. …”
Get full text

Characterizing Opine Physiological and Genetic Diversity in Moroccan strains of Allorhizobium vitis, the Causal Agent of Grapevine Crown Gall by Khaoula Habbadi, Hajar Hamzaoui, Imane Hourmatallah, Hiba Yahyaoui, Faiçal Aoujil, Majida Hafidi, Aziz Aziz, Salma El Iraqui El Houssaini

“…These results suggest a homogeneity of octopine/vitopine tumour-inducing plasmids (pTi), which contribute to the uniformity of opine-related genes. …”
Get full text

Expression and delivery of HA1-M2e antigen using an innovative attenuated Salmonella–mediated delivery system confers promising protection against H9N2 avian influenza challenge by Ram Prasad Aganja, In-Shik Kim, Hyun-Jin Tae, John Hwa Lee

“…To enhance immune protection against H9N2 avian influenza virus (AIV), which threatens poultry and humans, we used the previously constructed pJHL270 and pJHL305 plasmids with the Ptrc and CMV promoters to stimulate MHC class II and I responses through exogenous and endogenous antigenic presentation. …”
Get full text

Production of antibodies and antibody fragments containing non-natural amino acids in Escherichia coli by Jacquelyn Blake-Hedges, Dan Groff, Wilson Foo, Jeffrey Hanson, Elenor Castillo, Miao Wen, Diana Cheung, Mary Rose Masikat, Jian Lu, Young Park, Nina Abi Carlos, Hans Usman, Kevin Fong, Abigail Yu, Sihong Zhou, Joyce Kwong, Cuong Tran, Xiaofan Li, Dawei Yuan, Trevor Hallam, Gang Yin

“…Escherichia coli (E. coli) is a facile host for therapeutic nnAA protein synthesis because it can stably replicate plasmids encoding genes for product and nnAA incorporation. …”
Get full text

Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…The introduction of the system into the cell interior occurs with plasmids, RNA or ribonucleoproteins. A nuclear localization sequence allows CRISPR / Cas9 to enter the nucleus. …”
Get full text

Plasma Optimization as a Novel Tool to Explore Plant–Microbe Interactions in Climate Smart Agriculture by Binoop Mohan, Chandrima Karthik, Doni Thingujam, Karolina M. Pajerowska-Mukhtar, Vinoy Thomas, M Shahid Mukhtar

“…Furthermore, we discuss the optimization of plasma parameters for effective microbiome interaction and the role of plasmids in plant–microbe interactions. By characterizing plasmidome responses to plasma exposure and investigating transcriptional and metabolomic shifts, we provide insights into the potential of plasma as a tool for engineering beneficial plant–microbe interactions. …”
Get full text

Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water by Ashley D. Duffitt, Robert T. Reber, Andrew Whipple, Christian Chauret

“…Cells incubated in soil microcosms for 14 days expressed genes for antibiotic resistance, biosynthesis, DNA replication and modification, metabolism, phages, transposons, plasmids, pathogenesis and virulence, antibiotic resistance, ribosomal proteins, the stress response, transcription, translation, and transport and binding proteins at significantly higher levels than cells grown in Luria broth. …”
Get full text

Mechanisms of RCD-1 pore formation and membrane bending by Keli Ren, James Daniel Farrell, Yueyue Li, Xinrui Guo, Ruipei Xie, Xin Liu, Qiaozhen Kang, Qihui Fan, Fangfu Ye, Jingjin Ding, Fang Jiao

“…Abstract Regulator of cell death-1 (RCD-1) governs the heteroallelic expression of RCD-1-1 and RCD-1-2, a pair of fungal gasdermin (GSDM)-like proteins, which prevent cytoplasmic mixing during allorecognition and safeguard against mycoparasitism, genome exploitation, and deleterious cytoplasmic elements (e.g., senescence plasmids) by effecting a form of cytolytic cell death. …”
Get full text