TFE3 Regulates the Function of the Autophagy-Lysosome Pathway to Drive the Invasion and Metastasis of Papillary Thyroid Carcinoma by Hongsheng Lu, Chumeng Zhu, Yanyun Ruan, Lilong Fan, Kena Wei, Zhaohui Yang, Qi Chen

“…After inducing TFE3 overexpression by plasmid or TFE3 downregulation by small interfering RNA (siRNA) transfection, MTT, wound healing, and cell migration and invasion assays were used to verify the effects on invasion, migration, and the levels of autophagy-lysosome system-related proteins such as P62/SQSTM1, LC3, CTSL, and CTSB. …”
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Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p by Lei Xu, Gang Zhao, Hong Zhu, Shijun Wang, Aijun Sun, Yunzeng Zou, Junbo Ge

“…Retaining PPAR-γ in cytoplasm by transfecting with PPAR-γ⊿NLS plasmid in HUVECs failed to activate miR-590-5p. Mutation of the promoter region of PPAR-γ also reduced the miR-590-5p promoter luciferase activity. …”
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A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…Structural modeling assessed the potential impact of the variant. A mutant plasmid was constructed and transfected into 293 T cells. …”
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Immunogenicity of HIV-1 <i>Env</i> mRNA and <i>Env-Gag</i> VLP mRNA Vaccines in Mice by Qi Ma, Jing Yang, Xiaoguang Zhang, Hongxia Li, Yanzhe Hao, Xia Feng

“…BALB/c mice were immunized with env mRNA, env–gag VLP mRNA, env plasmid DNA vaccine, or lipid nanoparticle (LNP) controls. …”
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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

“…Based on the qualitative detection method, we established a relatively accurate quantitative detection method using the plasmid as a standard. Four water samples, totaling 64 samples, were analyzed using this method and compared with the qPCR method. …”
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Culturing of Giardia lamblia under microaerobic conditions can impact metronidazole susceptibility by inducing increased expression of antioxidant enzymes by Kateryna Starynets, Ana Paunkov, Anja Wagner, Klaus Kratochwill, Christian Klotz, David Leitsch

“…Indeed, overexpression of pyridoxamine 5′-phosphate oxidase in WB C6 from a plasmid carrying the respective gene behind the arginine deiminase promoter significantly improved growth of the transfected cell line under microaerobic conditions. …”
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VEGFR3 mitigates hypertensive nephropathy by enhancing mitophagy via regulating crotonylation of HSPA1L by Qiuwen Wu, Jiaxin Fu, Bin Zhu, Wei Meng, Jingyi Ma, Ying Lv, Wenqi Zhao, Fan Wang, Jingjin Liu, Yongshun Wang, Cong Peng, Shuo Zhang

“…Finally, the protective effect of VEGFR3 on mitophagy and oxidative stress were attenuated by transfection K130 (HSPA1L-K130R) mutant plasmid in vivo and in vitro. These findings indicated that VEGFR3 alleviated oxidative stress by promoting PARKIN-dependent mitophagy pathway via regulating HSPA1L crotonylation at K130 site in Ang II-induced PTCs, which provided a mechanistic basis for the therapeutic target in hypertensive renal injury.…”
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Recombinant Production of Bovine α_S1-Casein in Genome-Reduced <i>Bacillus subtilis</i> Strain IIG-Bs-20-5-1 by Lennart Biermann, Lea Rahel Tadele, Elvio Henrique Benatto Perino, Reed Nicholson, Lars Lilge, Rudolf Hausmann

“…However, the potential of genome-reduced <i>Bacillus subtilis</i> was applied for the recombinant production of bovine α_S1-casein protein. Methods: A plasmid-based gene expression system was established in <i>B. subtilis</i> allowing the production of his-tagged codon-optimized bovine α_S1-casein. …”
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Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens by Changjing Zhao, Cheng Yuan, Qingsen Zhao, Aihong Xia, Yaqing Dong, Shuying Xu, Shihan Chen, Yue Yuan, Yongjuan Wang

“…As templates for the sensitivity test, the recombinant plasmid standards were detected with a limit of 103 copies/μL or less. …”
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Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells by Junjie Wang, Dongdong Fu, Soulixay Senouthai, Yan Jiang, Rentong Hu, Yanwu You

“…HEK293T cells were cultured, and Cyr61 knockdown was achieved by transfection of the CRISPR/Cas9 KO plasmid. lncRNA and mRNA microarrays were used to identify differentially expressed genes (DEGs). …”
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A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy by N. P. Malakhova, Y. A. Skiba, G. A. Iskakova, D. A. Naizabayeva, B. K. Tezekbaeva, G. A. Ismagulova, E. R. Maltseva

“…The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. …”
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Molecular genetic detection and differentiation of <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i>, bacterial leaf streak agents of rice by M. L. Koroleva, S. A. Blinova, A. A. Shvartsev, V. E. Kurochkin, Ya. I. Alekseev

“…To evaluate the analytical sensitivity of the assay, a vector construct based on the pAL2-T plasmid was created through the insertion of X. o. pv. oryzicola target fragment (290 bp). …”
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Newly identified single-nucleotide polymorphism associated with the transition from nonalcoholic fatty liver disease to liver fibrosis: results from a nested case-control study in... by Yitong Ling, Yu Xuan Yang, Yan Chun Chen, Jing Hao Wang, Dong Ge Feng, Shi Jian Xiang, Xiaoyu Zhang, Jun Lyu, Sha Sha Li

“…Our procedures included cultivating these cells, employing SAMM50-rs2073080 plasmid techniques to enhance the expression of recently discovered SNPs, and conducting biochemical assays. …”
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Draft genome sequence of a co-harbouring blaNDM-5 and mcr-1.1 Escherichia coli phylogroup A isolate associated with patient colonisation in Ireland by Anna Tumeo, Francesca McDonagh, Aneta Kovarova, Kate Ryan, Christina Clarke, Georgios Miliotis

“…Forty-seven chromosomal VFs were identified, involved in adhesion and iron acquisition amongst other properties. Plasmid replicons associated with the spread of MDR genes such as IncHI2/HI2A were detected. wgMLST analysis showed the closest relative being a strain from the UK, exhibiting differences in the sequences of 851 genes. …”
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Study on the multidrug resistance and transmission factors of Staphylococcus aureus at the ‘animal–environment–human’ interface in the broiler feeding cycle by Fangyuan Hu, Fangyuan Hu, Yaopeng Liu, Yaopeng Liu, Lin Wang, Juan Wang, Na Liu, Yan Li, Xiaoxiao Duan, Junwei Wang, Mingzhe Lu, Junhui Liu, Zhina Qu, Keguang Han

“…The 42 representative strains carried mobile elements, mainly plasmid replicons (10 types), transposons (3 types), and 20 antibiotic resistance genes in 9 classes. …”
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Repressor Element 1 Silencing Transcription Factor (REST) Governs Microglia-Like BV2 Cell Migration via Progranulin (PGRN) by Tongya Yu, Yingying Lin, Yuzhen Xu, Yunxiao Dou, Feihong Wang, Hui Quan, Yanxin Zhao, Xueyuan Liu

“…On the contrary, REST was overexpressed by REST recombinant plasmid transfection, which repressed BV2 cell migration, indicating that REST may act as a repressor of cell migration. …”
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DNA PROTECTIVE ACTIVITY OF Artemisia spp HAIRY ROOT EXTRACTS by Duplij V., Afanasieva K., Chopei M., Matvieieva N. A.

“…At the same time, there were no significant differences in the degree of protection of plasmid DNA from damage (percentage of supercoiled DNA) when extracts from hairy root lines of A. vulgaris and A. tilesii were added to the reaction mixture. …”
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A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China by Zaixing Yang, Zaixing Yang, Yifan Li, Yuxuan Jiang, Yuxuan Jiang, Jingqi Wu, Zhenhong Guan, Junwei Ge, Lili Zhao

“…In this study, we developed a TaqMan probe-based real-time PCR technology (TaqMan-qPCR) for the specific, sensitive, and reproducible detection and quantification of AMDV in mink tissues by the VP2 gene, achieving detection limits as low as 1.69 × 101 copies/uL of plasmid DNA and 8.50 × 10−3 ng/uL of viral DNA, and the established TaqMan-qPCR assay is 100 times more sensitive than PCR. …”
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Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25 by Yuanyuan Ying, Fei Wu, Chongyang Wu, Yi Jiang, Min Yin, Wangxiao Zhou, Xinyi Zhu, Cong Cheng, Licheng Zhu, Kewei Li, Junwan Lu, Teng Xu, Qiyu Bao

“…The sequences sharing the greatest similarities to pLA-64 are the plasmids p02085-tetA of Citrobacter freundii and p234 and p388, both from Enterobacter cloacae. …”
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Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study by Zijuan Jian, Yanjun Liu, Zhiqian Wang, Peilin Liu, Jiahui Wang, Qun Yan, Wenen Liu

“…The epidemiological characteristics, virulome, resistome, plasmid replicons and two-component systems associated with colistin resistance of colistin-resistant isolates were explored by whole-genome sequencing. …”
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