Mechanistic studies on regulation of the activity of GPI-anchored serine protease testisin by WAN Jiahui, ZHOU Aiwu

“…Objective·To explore the activation methods and activity regulation mechanisms of the testisin zymogen in vitro, laying a foundation for further research on the physiological functions of testisin in organisms.Methods·The eukaryotic expression plasmid for the mouse-derived testisin (mTN) zymogen was constructed through full-gene synthesis and subsequently transfected into eukaryotic HEK293S cells for expression. …”
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Model systems of human immunodef iciency virus (HIV-1) for in vitro eff icacy assessment of candidate vaccines and drugs against HIV-1 by N. B. Rudometova, D. N. Shcherbakov, A. P. Rudometov, A. A. Ilyichev, L. I. Karpenko

“…The classical method of virology, which makes it possible to evaluate the neutralizing activity of the sera of animals immunized with experimental vaccines and the eff icacy of chemotherapy agents is the method of neutralization using viral isolates and infectious molecular clones, i. e. infectious viral particles obtained via cell transfection with a plasmid vector including the full-length HIV-1 genome coding structural, regulatory, and accessory proteins of the virus required for the cultivation of replication-competent viral particles in cell culture. …”
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PEDF Overexpression Ameliorates Cardiac Lipotoxicity in Diabetic Cardiomyopathy via Regulation of Energy Metabolism by Mao T, Wang Y

“…Additionally, H9c2 cells were transfected with a PEDF plasmid and cultured under HG+PA conditions (33 mm glucose + 250 μM palmitic acid) for 24 hours. …”
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Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris by Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti

“…Three copies of the lipase gene in pPIC9K_T1.2RQ(3x) recombinant plasmid were integrated into the genome of Pichia pastoris GS115, and quantitative analysis using qPCR technique confirmed that the GS115 transformant strain contained six copies of T1.2RQ gene, indicating two integration events. …”
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Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation by Huanjin Song, Hao Wu, Jun Dong, Sihua Huang, Jintao Ye, Ruoxi Liu

“…Then, TNF-α-treated MH7A cells were incubated with EA alone or together with MTA1 overexpression plasmid (pcDNA-MTA1), and we found that EA inhibited proliferation, inflammation cytokine levels, and oxidative stress marker protein levels and promoted apoptosis in MH7A cells, while MTA1 overexpression abolished these effects. …”
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INFORMACINIS GENOMAS, INDIVIDO IR POPULIACIJOS GENOMAI by Jonas Rubikas

“…Information genome is the DNA or RNA molecule carrying information for the beginning of replication (plasmid), for the replication of enzymes and other proteins and structures connected with the replication (viruses). …”
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INFORMACINIS GENOMAS, INDIVIDO IR POPULIACIJOS GENOMAI by Jonas Rubikas

“…Information genome is the DNA or RNA molecule carrying information for the beginning of replication (plasmid), for the replication of enzymes and other proteins and structures connected with the replication (viruses). …”
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N6-methyladenosine-modification of USP15 regulates chemotherapy resistance by inhibiting LGALS3 ubiquitin-mediated degradation via AKT/mTOR signaling activation pathway in hepatoce... by Ronghuan Fang, Zhigang Jia, Yuhang Xin, Kai Zhao, Wei Qin, Haoran Lu, Yi Zhou, Yongsheng Yang, He Fang

“…Functional analyses of immortalized human liver and HCC cell lines were conducted, including quantitative real-time PCR; western blot; plasmid, lentivirus, and siRNA transfection; co-immunoprecipitation; mass spectrometry; MeRIP-qPCR; and ubiquitination, cell growth, colony formation, and spheroid formation assays. …”
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Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells by Yueguo Wang, Zhenxing Ding, Youhui Tu, Xu Wu, Wenying Zhang, Shuang Ji, Jilong Shen, Li Zhang, Huimei Wu, Guanghe Fei

“…The small interfering RNA (siRNA) or plasmid targeting Nox4 was used to downregulate or upregulate Nox4, and the lentiviral vector encoding Poldip2 was used to downregulate or upregulate Poldip2. …”
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Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…The 2D PCR system underwent optimization, with its detection performance assessed in terms of specificity and sensitivity. Plasmid mixtures was used to simulate multiple infections of HPV, providing preliminary insights into the detection efficacy and throughput of the 2D PCR technology. …”
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Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB by Xia Huang, Yi Zeng, Yujie Jiang, Yueqiu Qin, Weigui Luo, Shulin Xiang, Suren R. Sooranna, Liao Pinhu

“…A549 cells were transfected with LBP or LBP shRNA plasmid DNA or pretreated with SB203580 or SC-514 following LPS treatment. …”
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Surveillance and characteristics of vancomycin-resistant Enterococcus isolates in a Chinese tertiary hospital in Shenzhen, 2018 to 2024 by Hongwei Shen, Qiaomin Zhang, Shaobo Li, Tingting Huang, Wen Ma, Daming Wang, Peng Li

“…Furthermore, an ongoing outbreak of ST80 and ST78 VREfm with vanA-harboring plasmid was identified. It is imperative that continuous surveillance be conducted in order to inform public health interventions.…”
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Inhibition of TFF3 synergizes with c-MET inhibitors to decrease the CSC-like phenotype and metastatic burden in ER+HER2+ mammary carcinoma by Chuyu He, Xuejuan Wang, Yi-Shiou Chiou, Basappa Basappa, Tao Zhu, Vijay Pandey, Peter E. Lobie

“…Targeting TFF3 with an interfering RNA plasmid or a small-molecule inhibitor (AMPC) inhibited these oncogenic characteristics, highlighting the therapeutic potential of targeting TFF3 in ER+HER2+ MC. …”
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Strategies for genetic manipulation of the halotolerant black yeast Hortaea werneckii: ectopic DNA integration and marker-free CRISPR/Cas9 transformation by Yainitza Hernandez-Rodriguez, A. Makenzie Bullard, Rebecca J. Busch, Aidan Marshall, José M. Vargas-Muñiz

“…Here, we report on two strategies to transform H. werneckii: ectopic integration of a plasmid and gene deletion using CRISPR/Cas9. These approaches will be fundamental to expanding the experimental techniques available to study H. werneckii, including live-cell imaging of cellular processes and reverse genetic approaches.…”
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Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T by Christian Suarez, Stefan Ratering, Torsten Hain, Moritz Fritzenwanker, Alexander Goesmann, Jochen Blom, Trinad Chakraborty, Boyke Bunk, Cathrin Spröer, Jörg Overmann, Sylvia Schnell

“…The E19T genome comprises one circular chromosome and one plasmid containing several genes involved in salt adaptation and genes related to plant growth-promoting traits under salt stress. …”
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Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells by Qiao Xie, Lie-ni Jia, Hong-yu Xu, Xiang-gang Hu, Wei Wang, Jun Jia

“…In this study, the bioactive PEI (polyethylenimine)/pBMP2- (bone morphogenetic protein-2 plasmid-) PLGA (poly(D, L-lactic-co-glycolic acid)) core-shell scaffolds were prepared using coaxial electrospinning for a controlled gene delivery to hPDLSCs (human periodontal ligament stem cells). …”
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Phenotypic and WGS-derived antibiotic resistance patterns of Salmonella Enteritidis isolates from retail meat and environment during 2014 to 2019 in China by Liya Zheng, Qiannan Di, Xuebin Xu, Liyuan Liu, Chunbo Qu, Phil Bremer, Phil Bremer, Xiujuan Zhou

“…The co-carrying of blaTEM, sul2 and aph(6)-Id/aph(3″)-Ib was likely mediated by an X1-type plasmid, corresponding to resistance against ampicillin, sulfisoxazole, and streptomycin. …”
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Astaxanthin Protects Primary Hippocampal Neurons against Noxious Effects of Aβ-Oligomers by Pedro Lobos, Barbara Bruna, Alex Cordova, Pablo Barattini, Jose Luis Galáz, Tatiana Adasme, Cecilia Hidalgo, Pablo Muñoz, Andrea Paula-Lima

“…To determine mitochondrial H2O2 production or NFATc4 nuclear translocation, neurons were transfected with plasmids coding for HyperMito or NFATc4-eGFP, respectively. …”
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Rapid detection of the invasive tomato leaf miner, Phthorimaea absoluta using simple template LAMP assay by Arindam Kumar, Damini Diksha, Susheel Kumar Sharma, P. R. Shashank, D. Nandhini, Soham Ray, Nitika Gupta, Mukesh Kumar Dhillon

“…The TLM-LAMP assay showed high sensitivity to P. absoluta DNA up to 1 × 10− 8 ng/µL and in plasmid DNA template up to 1 × 10–14 ng/µL. In addition, the TLM-LAMP assay was successful in laboratory detection of larvae, pupa, and adult stages of P. absoluta. …”
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Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing by Chun-Liang Li, Feng Qin, Rong-rong Li, Jia Zhuan, Hai-Yong Zhu, Yu Wang, Kai Wang

“…In this paper, PEI was covalently linked to chitosan, and the rat CGRP plasmid was encapsulated in a CS-PEI complex to construct CS-PEI/pCGRP nanoparticles. …”
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