Fragile Histidine Triad (FHIT) Suppresses Proliferation and Promotes Apoptosis in Cholangiocarcinoma Cells by Blocking PI3K-Akt Pathway by Qiang Huang, Zhen Liu, Fang Xie, Chenhai Liu, Feng Shao, Cheng-lin Zhu, Sanyuan Hu

“…To determine whether FHIT can regulate this pathway in cholangiocarcinoma QBC939 cells, we constructed an FHIT expression plasmid and used it to transfect QBC939 cells. Protein and mRNA expression were measured by western blotting and qRT-PCR, respectively. …”
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Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case by Zoltán Tóth, Bence Balázs, Walter P. Pfliegler, Eszter Csoma, László Majoros, Dorka Szűcs, Renátó Kovács

“…Based on the WGS data, the isolate belonged to the K1-capsule-type ST23, harboured a pNTUH-2044-like plasmid, and was positive for all the virulence factors associated with this lineage. …”
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Codon optimized influenza H1 HA sequence but not CTLA-4 targeting of HA antigen to enhance the efficacy of DNA vaccines in an animal model by Dito Anurogo, Chia-Yuan Chen, Chu-Chi Lin, Jeanne Adiwinata Pawitan, Daniel W. Qiu, J. Timothy Qiu

“…In cytokine assays, all plasmid constructs, particularly pCTLA4-cHA, induced robust interferon (IFN)-γ production, while interleukin (IL)-4 levels remained uniformly non-significant. …”
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Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes by Esther López, M. Paz Zafra, Beatriz Sastre, Cristina Gámez, Carlos Lahoz, Victoria del Pozo

“…In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation. …”
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Upregulation of TCPTP in Macrophages Is Involved in IL-35 Mediated Attenuation of Experimental Colitis by Baoren Zhang, Chenglu Sun, Yanglin Zhu, Hong Qin, Dejun Kong, Jingyi Zhang, Bo Shao, Xiang Li, Shaohua Ren, Hongda Wang, Jingpeng Hao, Hao Wang

“…Mice were treated with Pichia/IL-35, empty plasmid-transformed Pichia (Pichia/0), or PBS by gavage, respectively. …”
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Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. by Junaid M Alam, Eric N Riha, Haris Ahmed, Hong M Thai, Kevin Garnepudi, Ramesh B Kesavan, Gnananandh Jayaraman, Anna Sangster, Dylan Curry, Heidi A Butz, Lori Smith, Maureen Vowles, Kelly F Oakeson, Erin L Young, Siva T Sarva

“…Both isolates had yersiniabactin, colibactin, aerobactin and salmochelin producing loci which likely confer these isolates hvKp phenotype. 2020CK-00441 and 2021CK-00720 had a unique pK2044 like plasmid.<h4>Conclusions</h4>HvKp strains capable of causing devastating metastatic septic infections have emerged in Texas. …”
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GPX3-Mediated Oxidative Stress Affects Pyrimidine Metabolism Levels in Stomach Adenocarcinoma via the AMPK/mTOR Pathway by Yaowen Zhang, Yixin Yang, Shanshan Kuang, Yang Zhang, Hancheng Qin, Jisheng Xie

“…Functional studies using an overexpressed GPX3 plasmid showed an enhanced migration and invasion of STAD cells as well as the expression of genes associated with pyrimidine metabolism and the AMPK/mTOR signaling pathway. …”
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Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils by Daria Augustyniak, Justyna Roszkowiak, Izabela Wiśniewska, Jacek Skała, Daiva Gorczyca, Zuzanna Drulis-Kawa

“…Next, using hBD-2-pGL4[luc2] plasmid with luciferase reporter gene, SP and CGRP were shown to inhibit the IL-1β-dependent expression of potent neutrophil chemoattractant, hBD-2 defensin, in transfected A549 epithelial cells (type II alveolar cells) upon OMV stimulation. …”
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Generation of transgenic chicken through ovarian injection by Jinghua Jiang, Caiying Wang, Xuguang Du, Fei Gao, Sen Wu

“…The injection of 20 μg plasmid yielded the highest transgenic efficiency at 12.1%. …”
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The Kat in the HAT: The Histone Acetyl Transferase Kat6b (MYST4) Is Downregulated in Murine Macrophages in Response to LPS by Smita Shukla, Carly Levine, Roopa Payanur Sripathi, Genie Elson, Carol Susan Lutz, Samuel Joseph Leibovich

“…Using the pLightswitch 3′UTR reporter plasmid, the miR-487b binding site in the Kat6b 3′UTR was found to play a role in the LPS-mediated suppression of Kat6B expression, but other as-yet unidentified factors are also involved. …”
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Genomic and phylogenetic analysis of a NDM-1 producing ST152 Klebsiella pneumoniae isolated from a bloodstream infection in China by Renchi Fang, Lingfang Di, Xuebin Tian, Yongfeng Bai

“…The blaNDM-1 gene was identified on the IncX3 plasmid pHZKP1-NDM (54,035 bp), located downstream of the ISKox3-IS3000-ISAba125-IS5 segment and upstream of the bleMBL-IS26-blaSHV-69-IS26 segment. …”
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Extracellular Vesicles and Tunnelling Nanotubes as Mediators of Prostate Cancer Intercellular Communication by Jessica K. Heatlie, Joanna Lazniewska, Courtney R. Moore, Ian R. D. Johnson, Bukuru D. Nturubika, Ruth Williams, Mark P. Ward, John J. O’Leary, Lisa M. Butler, Doug A. Brooks

“…Overexpression assays of a newly designed androgen receptor (AR) plasmid revealed its novel localization in PCa cellular vesicles, which were also transferred to neighbouring cells. …”
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Developing a framework for tracking antimicrobial resistance gene movement in a persistent environmental reservoir by Amy J. Mathers, Thomas J. X. Li, Qijun He, Sharvari Narendra, Nicole Stoesser, David W. Eyre, A. Sarah Walker, Katie E. Barry, Salvador Castañeda-Barba, Fenix W. Huang, Hardik Parikh, Shireen Kotay, Derrick W. Crook, Christian Reidys

“…From the validated model, we demonstrate frequent transposition events of bla KPC from plasmids to other plasmids, as well as integration into the bacterial chromosome within specific drains. …”
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CXC chemokine receptor 7 ameliorates renal fibrosis by inhibiting β-catenin signaling and epithelial-to-mesenchymal transition in tubular epithelial cells by Ping Meng, Chunli Liu, Jingchun Li, Ping Fang, Bo Yang, Wei Sun, Yunfang Zhang

“…Administration of pFlag-CXCR7, an overexpression plasmid for CXCR7, significantly inhibited the activation of β-catenin signaling and protected against the progression of epithelial-to-mesenchymal transition (EMT) and renal fibrosis in a UIRI mouse model. …”
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A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis by Reyhaneh Sadat Moosavi-Kohnehsari, Mahnaz Jafari-Sohi, Tohid Piri-Gharaghie, Shakiba Tolou-Shikhzadeh-Yazdi, Mona Aghassizadeh-Sherbaf, Romina Hosseinzadeh

“…The genetically engineered L. lactis was given orally to birds as a vaccine after a multi-epitope vector was created using a reverse vaccinology technique. After the plasmid was digested, the target group produced a 72 kDa protein called multi-epitop. …”
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A Ferritin-Based Eg95 Nanoparticle Vaccine Adjuvanted with pCpG Eliciting Robust Immune Responses Against Cystic Echinococcosis in Mice Model by Gao X, Zhu X, Liu X, Zhou C, Shang Y, Wu T, Jia H, Zhang Z, Li Y, Xin T

“…The commercial Quil-A-adjuvanted Eg95 vaccine was empirically effective for CE control; however, it is expensive and has side effects and insufficient immunity.Purpose: This study aimed to employ a novel adjuvant consisting of a delivery system and an immune potentiator and assess its adjuvanticity to Eg95 antigen, thereby developing a safe and cost-effective novel vaccine against the disease.Methods: A ferritin-based Eg95 nanoparticle antigen was prepared and then mixed with a plasmid containing the TLR9 agonist CpG to formulate a novel nanovaccine. …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…To decrease the immunogenicity of this protein, a recombinant plasmid coding for shortened TNF-BD protein of VARV in Escherichia coli cells has been constructed. …”
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Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study by Qin Wang, Sayyed Salman, Ye Luo, XiaoMei Tong

“…Additionally, the genome harbours three antibiotic resistance genes and a newly discovered plasmid, pL3897_NDM, highlighting the need for surveillance of drug-resistant pathogens. …”
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miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading Keap1 by Wei Geng, Shaohua Yan, Xinyue Li, Qiumei Liu, Xuefei Zhang, Xinshun Gu, Xiang Tian, Yunfa Jiang

“…The mimic of miR-432-5p plasmid was constructed to verify the effect of miR-432-5p on ferroptosis. …”
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Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens by Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue

“…The assay’s detection limit was 5 copies/μL of plasmid and 4 CFU/μL of bacterial DNA. Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. …”
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