Complete genome sequence of Pseudomonas aeruginosa YK01, a sequence type 16 isolated from a patient with keratitis by Shuo Jiang, Mengmin Ye, Ke Liu, Huiluo Cao, Xiaoshan Lin

“…The assembly is fully complete with chromosomal genome size of 6,183,266 bp, presenting a GC content of 66.7%, and a plasmid with the size of 46,067 bp, presenting GC content of 59.0%. …”
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Association of qnr Genes and OqxAB Efflux Pump in Fluoroquinolone-Resistant Klebsiella pneumoniae Strains by Fereshteh Amereh, Mohammad Reza Arabestani, Seyed Mostafa Hosseini, Leili Shokoohizadeh

“…The aim of this study was to investigate the frequency and relationship between plasmid-mediated quinolone resistance genes and OqxAB pump genes, as well as the genetic linkage in K. pneumoniae strains isolated from Hamadan hospitals in the west of Iran. …”
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Investigating the Existence of Ribosomal Protein L5 Gene in Syrian Strain of Leishmania tropica Genome: Sequencing It and Evaluating Its Immune Response as DNA Vaccine by Mohammad Maarouf, Alyaa A. Abdlwahab

“…After proving the existence of the ribosomal protein L5 gene in a Syrian strain of Leishmania tropica (LCED Syrian 01), it was sequenced and cloned into a pCI plasmid, and the designed DNA vaccine was administered to BALB/c mice. …”
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Severe Wound Infection with Photobacterium damselae ssp. damselae and Vibrio harveyi, following a Laceration Injury in Marine Environment: A Case Report and Review of the Literatur... by Jörg Hundenborn, Steffi Thurig, Mechthild Kommerell, Heike Haag, Oliver Nolte

“…The isolate tested positive for pPHDD1, a plasmid conferring high-level virulence. Furthermore, the wound was coinfected with Vibrio harveyi, a halophile bacterium, which has never been reported from human infections before. …”
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Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice by E. Hattinger, S. Scheiblhofer, E. Roesler, T. Thalhamer, J. Thalhamer, R. Weiss

“…Recently, mRNA vaccines have been introduced as a safety-optimized alternative to plasmid DNA-based vaccines for protection against allergy. …”
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LysR-Type Transcriptional Regulator VirR Responds to Temperature and pH and Directly Activates the Transcription of virS-Containing Operon in Rhodococcus equi by Tsutomu Kakuda, Takashi Sato, Mari Takuhara, Hirofumi Hagiuda, Yasunori Suzuki

“…Virulent strains of R. equi possess a virulence-associated plasmid, which encodes a 15- to 17-kDa surface protein called virulence-associated protein A (VapA). …”
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A Constitutively Mannose-Sensitive Agglutinating Salmonella enterica subsp. enterica Serovar Typhimurium Strain, Carrying a Transposon in the Fimbrial Usher Gene stbC, Exhibits Mul... by Kuan-Hsun Wu, Ke-Chuan Wang, Lin-Wen Lee, Yi-Ning Huang, Kuang-Sheng Yeh

“…A complementation test revealed that transforming a recombinant plasmid possessing the stbC restored the mannose-sensitive agglutination phenotype to the stbC mutant much as that in the parental Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.…”
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BMSCs Regulate Astrocytes through TSG-6 to Protect the Blood-Brain Barrier after Subarachnoid Hemorrhage by Yilv Wan, Min Song, Xun Xie, Zhen Chen, Ziyun Gao, Xiang Wu, Rui Huang, Min Chen

“…BMSCs were transfected with TSG-6 inhibitor plasmid and cocultured with astrocytes. Intravenous transplantation of BMSCs was utilized to treat SAH rats. …”
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Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction by Thiago dos Santos Gomes, Mariana Coimbra Garcia, Flavia de Souza Cunha, Heloisa Werneck de Macedo, José Mauro Peralta, Regina Helena Saramago Peralta

“…For E. hartmanni, the Tm was 73°C and the amplification was successful down to 0.03 fg of plasmid DNA. Negative controls and other intestinal parasites presented no amplification. …”
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The 256 kb mitochondrial genome of Clavaria fumosa is the largest among phylum Basidiomycota and is rich in introns and intronic ORFs by Xu Wang, Yajie Wang, Wen Yao, Jinwen Shen, Mingyue Chen, Ming Gao, Jiening Ren, Qiang Li, Na Liu

“…Large intergenic regions, intronic regions, accumulation of repeat sequences and plasmid-derived genes together promoted the size expansion of the C. fumosa mitogenome. …”
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Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model by Alireza Vasiee, Fereshteh Falah, Mojtaba Sankian, Farideh Tabatabaei-Yazdi, Seyed Ali Mortazavi

“…The main gene of this allergen, Ama r 2, was cloned into the food grade plasmid pNZ7025 and then was electrotransformed into the food grade Lactococcus lactis NZ1330. …”
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Utilizing the subtractive proteomics approach to design ensemble vaccine against Candida lusitaniae for immune response stimulation; a bioinformatics study. by Habiba Naz, Rimsha Timotheous, Muhammad Farhan Sarwar, Tariq Nadeem, Mudassar Fareed Awan, Sajed Ali, Sophia Awais, Irfan Ahmed

“…Afterwards, the codon optimization was executed, followed by in-silico cloning of the final vaccine construct. The pet28A plasmid was incorporated for this purpose while, the SnapGene tool was utilized for this particular analysis. …”
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Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients by Shinwan Kany, Johann-Philipp Horstmann, Ramona Sturm, Katharina Mörs, Borna Relja

“…Transfection of monocytes with NLRP3-encoding plasmid recovered the functionality of monocytes from TP regarding the IL-1β secretion. …”
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Neuron-Specific Fluorescence Reporter-Based Live Cell Tracing for Transdifferentiation of Mesenchymal Stem Cells into Neurons by Chemical Compound by Do Won Hwang, Hyun Woo Kwon, Jaeho Jang, Hee Jung Jung, Kwang Rok Kim, Dong Soo Lee

“…In this study, using time-lapse live cell imaging, we assessed the neuronal differentiation of MSCs induced by a small molecule “NHPDQC (N-hydroxy-2-oxo-3-(3-phenylprophyl)-1,2-dihydroquinoxaline-6-carboxamide, C18H17N3O3).” Plasmid vector containing red fluorescence reporter genes under the control of the tubulin α1 (Tα1) promoter (pTα1-DsRed2) traced the neuronal differentiation of MSCs. …”
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Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies by Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Rita Lasickienė, Artiomas Akatov, Gabrielis Kundrotas, Vilimas Sereika, Raimundas Lelešius, Aurelija Žvirblienė, Kęstutis Sasnauskas

“…The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and inserted into yeast Saccharomyces cerevisiae expression plasmid. Recombinant PPV VP2 protein was efficiently expressed in yeast and purified using density gradient centrifugation. …”
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Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma by Haideh Namdari, Farhad Rezaei, Fatemeh Heidarnejad, Mohammad Yaghoubzad-Maleki, Maryam Karamigolbaghi

“…In silico codon optimization and cloning of the vaccine nucleic acid sequence were accomplished using the pET28a plasmid. Furthermore, this vaccine displayed the capacity to modulate humoral and cellular immune responses. …”
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Comparison of chicoric acid, and its metabolites caffeic acid and caftaric acid: In vitro protection of biological macromolecules and inflammatory responses in BV2 microglial cells by Qian Liu, Fan Liu, Liling Zhang, Yajie Niu, Zhigang Liu, Xuebo Liu

“…Moreover, CA, CTA and CFA all exerted remarkable inhibition capacities on linoleic acid and soybean lecithin liposomes peroxidation in a dose-dependent manner, and restrained the oxidation of herring sperm DNA, as well as the breakage of pBR322 plasmid DNA. Furthermore, CA and its metabolites suppressed lipopolysaccharide-induced decline of BV2 cell viability and the production of NO and ROS. …”
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Two cellular models for analyzing mitochondrial heteroplasmy by Yaning Hu, Lin Jiang, Wen Liu, Xingbo Zhao

“…Methods: Model 1: the yak cell line (Bos grunniens) was transfected with p-eGFP-neo plasmid while mammary alveolar cell-T (MAC-T) cell line from cattle cells (Bos taurus) was stained with MitoTracker Deep Red FM. …”
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Leveraging Next-Generation Sequencing Application from Identity to Purity Profiling of Nucleic Acid-Based Products by Rucha Wadapurkar, Swarda Deo, Renuka Khanzode, Ajay Singh

“…<b>Results</b>: Our workflow predicted most of the critical quality controls of mRNA vaccine and plasmid DNA samples, especially focusing on the identity and the nucleotide-based impurities. …”
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Heterologous Ectoine Production in Escherichia coli: Optimization Using Response Surface Methodology by I Putu Parwata, Deana Wahyuningrum, Sony Suhandono, Rukman Hertadi

“…The ectoine gene cluster from the halophilic bacterium was isolated and inserted into an expression plasmid of pET30(a) and subsequently transformed into E. coli BL21 (DE3). …”
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