Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates by Amin Hadi, Abbas Rastgoo, Maryam Eskandarian, Nooshin Haghighipour, Azam Bolhassani

“…Cell viability and low nucleic acid expression are the problems related to dendritic cells (DCs) and mesenchymal stem cells (MSCs), which are transfected with plasmid DNA. Thus, novel in vitro strategies are needed to improve DNA transfection into these cells. …”
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Biodesulfurization enhancement via targeted re-insertion of the flavin reductase dszD in the genome of the model strain Rhodococcus qingshengii IGTS8 by Olga Martzoukou, Fotios Klenias, Eleni Kopsini, Dimitris G. Hatzinikolaou

“…The model desulfurizing strain Rhodococcus qingshengii IGTS8 is responsible for the removal of sulfur through the 4S metabolic pathway, operating through a plasmid-borne dszABC operon, as well as the chromosomal gene for the flavin reductase, dszD. …”
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A Helicobacter pylori flagellar motor accessory is needed to maintain the barrier function of the outer membrane during flagellar rotation. by Kyle Rosinke, Shoichi Tachiyama, Jan Mrásek, Jun Liu, Timothy R Hoover

“…Deletion analysis of hp0838 in H. pylori B128 showed that the gene is not required for motility in soft agar medium, but the mutant displayed a reduced growth rate and an increased sensitivity to bacitracin, which is an antibiotic that is normally excluded by the outer membrane. Introducing a plasmid-borne copy of hp0838 into the H. pylori Δhp0838 mutant suppressed the fitness defect and antibiotic sensitivity of the strain. …”
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Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells by Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong

“…FTO overexpression was achieved by transiently transfecting an FTO overexpression plasmid into the HCT15 SW480 colorectal cancer cell line. …”
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DNA Cleavage, Cytotoxic Activities, and Antimicrobial Studies of Ternary Copper(II) Complexes of Isoxazole Schiff Base and Heterocyclic Compounds by Vijay Kumar Chityala, K. Sathish Kumar, Ramesh Macha, Parthasarathy Tigulla, Shivaraj

“…The DNA cleavage studies have also been carried out on pBR 322 and it is observed that these Cu(II) complexes are capable of cleaving supercoiled plasmid DNA in the presence of H2O2 and UV light.…”
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Expression of Helicobacter pylori hspA Gene in Lactococcus lactis NICE System and Experimental Study on Its Immunoreactivity by Xiao-Juan Zhang, Shu-Ying Feng, Zhi-Tao Li, Yan-Ming Feng

“…After L. lactis NZ3900/pNZ8110-hspA was constructed, growth curves were plotted to study whether the growth of recombinant L. lactis was affected after hspA was cloned into L. lactis and whether the growth of empty bacteria, empty plasmid bacteria, and recombinant L. lactis was affected by different concentrations of Nisin; SDS-PAGE and Western blot were adopted, respectively, to detect the HspA expressed by recombinant L. lactis and its immunoreactivity. …”
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The Transcription Factor Creb is Involved in Sorafenib-Inhibited Renal Cancer Cell Proliferation, Migration and Invasion by Huang Shuaishuai, Cui Pinger, Lin Shuangxia, Yao Xuping, Wang Xue, Ren Yu, Weng Guobin

“…Moreover, addition of the recombinant plasmid pCI-neo/ CREB (PN) reversed the sorafenib-induced inhibition of cell proliferation, migration and invasion. …”
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Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts by Adelina Simamora, Adit W. Santoso, Kris H. Timotius, Ika Rahayu

“…The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. …”
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Potential links between human bloodstream infection by Salmonella enterica serovar Typhimurium and international transmission to Colombia. by Yan Li, Caisey V Pulford, Paula Díaz, Blanca M Perez-Sepulveda, Carolina Duarte, Alexander V Predeus, Magdalena Wiesner, Darren Heavens, Ross Low, Christian Schudoma, Angeline Montaño, Neil Hall, Jaime Moreno, Jay C D Hinton

“…We used whole-genome sequencing to analyse multidrug-resistant (MDR) profiles, plasmid distribution, and to define phylogenetic relationships. …”
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One-Step Formation of Chondrocytes through Direct Reprogramming via Polysaccharide-Based Gene Delivery by Qingtong Yu, Yan Wang, Xia Cao, Wenwen Deng, Michael Adu Frimpong, Jiangnan Yu, Ximing Xu

“…Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. …”
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Purification and expression of a novel bacteriocin, JUQZ-1, against Pseudomonas syringae pv. Actinidiae (PSA), secreted by Brevibacillus laterosporus Wq-1, isolated from the rhizos... by Yang Shuai, Yi Langbo, Yang Yi, Chen Danni, Peng Qingzhong

“…The gene of the 01021 protein was cloned into the plasmid pPICZa, and the recombinant bacteriocin was successfully expressed using the Pichia pastoris X33 expression system. …”
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Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p by Mei Zheng, Panpan Fan, Pu Yang, Junwen Zheng, Dongchi Zhao

“…The immunofluorescence analysis showed that the plasmid-mediated NS1 protein was observed in both the cytoplasm and nucleus. …”
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Development and characterization of a reverse genetics system for the lineage II Chicava strain of Machupo virus in a guinea pig model. by Emily Mantlo, Junki Maruyama, John T Manning, Rachel A Reyna, Cheng Huang, Slobodan Paessler

“…<h4>Methods</h4>We report the development of a reverse genetics system for the MACV lineage II Chicava strain, using a pRF42 plasmid encoding the L and S segment genomic RNA under the transcriptional control of a murine DNA-dependent RNA polymerase I promoter sequence. …”
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Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System by Pelin Kolçak Yaşlı, Seda Kulabaş, Evren Doruk Engin

“…In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. …”
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Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani by Silvana Pompéia Val-Moraes, Eliamar Aparecida Nascimbem Pedrinho, Eliana Gertrudes Macedo Lemos, Lucia Maria Carareto-Alves

“…This metagenomic DNA was used as a template to amplify fungal 18S rDNA sequences, and libraries were constructed in Escherichia coli by cloning PCR products. The plasmid inserts were sequenced and compared to known rDNA sequences in the GenBank database. …”
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Occurrence of Bordetella Infection in Pigs in Northern India by Sandeep Kumar, Bhoj R. Singh, Monika Bhardwaj, Vidya Singh

“…Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile. The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively. …”
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The Human IL-22 Receptor Is Regulated through the Action of the Novel E3 Ligase Subunit FBXW12, Which Functions as an Epithelial Growth Suppressor by Joseph Franz, Jacob Jerome, Travis Lear, Qiaoke Gong, Nathaniel M. Weathington

“…FBXW12 causes depletion of endogenous and plasmid-derived IL-22R in lung epithelia, binds the E3 ligase constituent Skp-1, and facilitates ubiquitination of IL-22R in vitro. …”
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Cu(II) and Ni(II) Complexes with New Tridentate NNS Thiosemicarbazones: Synthesis, Characterisation, DNA Interaction, and Antibacterial Activity by Dorian Polo-Cerón

“…Cu(II) and Ni(II) complexes can wind DNA strands through groove interactions and promote strand breakage of the plasmid pmCherry under oxidative stress conditions. …”
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Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt by Shaymaa H. Abdel-Rhman, Rehab M. Elbargisy, Dina E. Rizk

“…Only scarce studies investigated the association between plasmid-mediated quinolone resistance (PMQR) genes and integrons in clinical isolates of E. coli. …”
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HMGB1 Box A gene therapy to alleviate bleomycin-induced pulmonary fibrosis in rats by Rathasapa Patarat, Suchanart Chuaybudda, Sakawdaurn Yasom, Apiwat Mutirangura

“…Previously, a new gene therapy technology using Box A of the HMGB1 plasmid (Box A) was used to reverse cellular senescence and cure liver fibrosis in aged rats. …”
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