A CRISPR-Cas9 system protecting E. coli against acquisition of antibiotic resistance genes by Danna Lee, Petra Muir, Sara Lundberg, August Lundholm, Linus Sandegren, Sanna Koskiniemi

“…Furthermore, we show that the system blocks conjugation of a set of clinical plasmids, and that the system is also able to protect the probiotic bacterium E. coli Nissle 1917 from acquiring AMR genes.…”
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Modulating gene expression as a strategy to investigate thyroid cancer biology by Diego Claro de Mello, Joice Moraes Menezes, Antonio Tarelo Freitas de Oliveira, Marcella Maringolo Cristovão, Edna Teruko Kimura, Cesar Seigi Fuziwara

“…In this review, we cover the most frequently used current techniques for gene modulation in the thyroid cancer field, such as RNA interference (RNAi), short hairpin RNA (shRNA), and gene editing with CRISPR/Cas9 for inhibiting a target gene, and strategies to overexpress a gene, such as plasmid cloning and CRISPRa. Exploring the possibilities for gene modulation allows the improvement of the scientific quality of the studies and the integration of clinicians and basic scientists, leading to better development of translational research.…”
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First Description of KPC-2-Producing Klebsiella oxytoca Isolated from a Pediatric Patient with Nosocomial Pneumonia in Venezuela by Indira Labrador, María Araque

“…The isolate LMM-SA26 carried a ~21 kb conjugative plasmid that harbored the blaKPC-2, blaCTX-M-8, and blaTEM-15 genes. …”
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HDAC1 and HDAC2 Are Involved in Influenza A Virus-Induced Nuclear Translocation of Ectopically Expressed STAT3-GFP by Jessica Leong, Matloob Husain

“…We employed a quantitative confocal microscopy approach and analyzed the nuclear translocation of plasmid-expressed STAT3-GFP in IAV-infected cells depleted with the expression of HDAC1 or HDAC2. …”
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Researchizs on biochemical, antibiotics sensitivity and transferable drug - resistance ( r - factor) characteristics of e. c ali strains isolated from diarrheal calves in konya reg... by Mehmet ATEŞ, İ. Yavuz SEZEN, Osman ERGANİŞ, Mehmet ÇORLU

“…<p> The rates of R &#8211; plasmid transmission of the strain were found to be 27.1 per cent.…”
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DEPENDENCE OF A GAS-PHASE DNA GLOBULE SIZE ON CHAIN LENGTH by T. N. Goryachkovskaya, A. S. Kozlov, V. M. Popik, N. A. Kolchanov, S. E. Peltek

“…Comparison of diffusion sizes of plasmid pUC18 aerosol particles with those obtained by AFM indicated that DNA molecules experienced condensation in the gas phase. …”
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Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish by . Alimuddin, G. Yoshizaki, O. Carman, T. Takeuchi

“…Pada penelitian ini, kami melakukan seleksi kontruksi plasmid yang tepat yang menghasilkan tingkat ekspresi yang tinggi dari gen D6-desaturase-like ikan masu salmon (Oncorhynchus masou) yang ditransfer ke ikan zebra (Danio rerio) untuk meningkatkan kemampuannya dalam mensintesa EPA/DHA.  …”
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CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE by Ali Mazloum, N. G. Zinyakov, A. S. Igolkin, N. N. Vlasova

“…Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. …”
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Tree polynomials identify a link between co-transcriptional R-loops and nascent RNA folding. by Pengyu Liu, Jacob Lusk, Nataša Jonoska, Mariel Vázquez

“…By applying this pipeline to plasmid sequences that contain R-loop forming genes, we establish a strong correlation between the coefficient sums of tree-polynomials and the experimental probability of R-loop formation. …”
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Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae: Possible Role in the Entry into Its Host Cells by Tuula A. Peñate Medina, Juha T. Korhonen, Riitta Lahesmaa, Mirja Puolakkainen, Oula Peñate Medina, Paavo K. J. Kinnunen

“…SMase activity was measured in vitro from isolated C. pneumoniae elementary bodies (EB) and in the lysate from E. coli cells transfected with a plasmid expressing CPn0300 protein having sequence similarity to SMase. …”
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Molecular Characterization of Antibiotic Resistance and Genetic Diversity of Klebsiella pneumoniae Strains by Bahareh Arabzadeh, Zeynab Ahmadi, Reza Ranjbar

“…PCR was used to amplify the gyrA gene in quinolone-resistant isolates and sequencing was performed for the detection of probable mutations between the isolates. The occurrence of plasmid-mediated quinolone resistance genes (qnrA, qnrB, and qnrS) was also investigated by PCR. …”
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Cotrimoxazole-Resistant Escherichia coli Bacteremia in Neutropenic Patients at a Regional Oncology Hospital by Daniel B Gregson, Anne G Matlow, Andrew E Simor, Peter G Tuffnell, Donald E Low, Ronald Feld, James E Brunton

“…In 1988, 11 of 32 Gram-negative rod bacteremias were secondary to other strains of indole positive E coli of several different biotypes and plasmid profiles. Indole negative strains all exhibited low level trimethoprim resistance, whereas indole positive strains which subsequently appeared exhibited high level trimethoprim resistance. …”
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CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE by Ali Mazloum, N. G. Zinyakov, A. S. Igolkin, N. N. Vlasova

“…Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. …”
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RECONSTRUCTION OF MECHANISMS REGULATING THE EXPRESSION OF THE ESCHERICHIA COLI YFIA GENE UNDER STRESS CONDITIONS by T. M. Khlebodarova, D. Yu. Oshchepkov, N. V. Tikunova, I. V. Babkin, A. D. Gruzdev, V. A. Likhoshvai

“…Simulation of the response of E. coli cells transformed with pYfi-gfp plasmid to oxidative stress indicated that the maximum agreement with experimental data was achieved in a model implying a complex action, apparently mediated by several transcription factors (TFs). …”
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Current understanding of antimicrobial resistance mechanisms in bacteria (analytical review) by O. V. Pruntova, V. S. Russaleyev, N. B. Shadrova

“…Horizontal gene transfer, including free DNA transformation, transduction by bacteriophages and plasmid-involving conjugation, is believed to play an important role in antimicrobial resistance spread. …”
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Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2 by Shi-Weng Li, Meng-Yuan Liu, Rui-Qi Yang

“…The genome of strain DM2 consists of a circular chromosome of 4,238,631 bp for 4458 protein-coding genes and a plasmid of 84,240 bp coding for 103 genes. Thirty-four genomic islands coding for 330 proteins and 5 prophages are found in the genome. …”
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Blocking REDD1/TXNIP Complex Ameliorates HG-Induced Renal Tubular Epithelial Cell Apoptosis and EMT through Repressing Oxidative Stress by Lin Mu, Nan Chen, Yakun Chen, Zhifen Yang, Huandi Zhou, Shan Song, Yonghong Shi

“…Similarly, the transfection of REDD1 siRNA plasmid also suppressed HG-induced apoptosis and EMT. …”
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IL-17 Mildly Rescued the Impaired Proliferation of Alveolar Epithelial Cells Induced by LCN2 Overexpression by Tingting Lv, Ziliang Hou, Kaiyuan Yang, Jinxiang Wang

“…Moreover, human pulmonary alveolar epithelial cells (HPAEpiCs) were transfected with the LCN2 overexpression plasmid vector in vitro. Recombinant human interleukin-17 (IL-17) protein (rhIL-17) at different concentrations was administered to intervene in HPAEpiCs, observing cell viability and analyzing the concentration-dependent effect of IL-17. …”
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RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis by Xinqing Wang, Hao Fan, Xiaoping Ye, Yu Hu, Yan Xiao, Ming Zhang, Yonghui Xu, Jianjun Song, Yongyun Luo

“…DAZAP1 knockdown was achieved through plasmid transfection, and its effects on ferroptosis and PC progression were evaluated using RT–qPCR, western blotting, CCK-8 assays, EdU staining, Fe2+ content measurement, reactive oxygen species (ROS) detection, wound healing and Transwell migration assays. …”
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Obtaining a pancreatic cancer cell line stably expressing doxycycline-dependent endonuclease Cas9 by A.K. Nurgalieva, V.S. Skripova, L.F. Minigulova, R.G. Kiyamova

“…The aim of this work is modification of the pancreatic cancer cell line MIA PaCa-2 to express a doxycycline-inducible Cas9 endonuclease gene. Several plasmid vectors have been used to create a lentivirus containing the Cas9 endonuclease gene. …”
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