Fufang E’jiao Jiang’s effect on immunity, hematopoiesis, and angiogenesis via a systematic “compound-effect-target” analysis by Xiang Li, Xiao Xu, Ying Dong, Shusheng Fan, Xueyang Ren, Yuan Zheng, Jiamu Ma, Feng Zhang, Qingyue Deng, Xianxian Li, Yingyu He, Mengyu Sun, Wei Liu, Mengxia Li, Qing Xia, Yan Zhang, Gaimei She

“…Transcriptome data showed that several pathways such as complement and coagulation cascades, ECM-receptor interaction, and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ. 19 common targets were obtained through combined analysis of network pharmacology and transcriptomics, and 5 targets of them were verified by PCR. …”
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Impact of a positive end-expiratory pressure on oxygenation, respiratory compliance, and hemodynamics in obese patients undergoing laparoscopic surgery in reverse Trendelenburg pos... by Gulfairus A. Yessenbayeva, Aizhan M. Meyerbekova, Sergey I. Kim, Murat B. Zhumabayev, Gulbanu S. Berdiyarova, Sanzhar B. Shalekenov, Dinara S. Zharlyganova, Irina Y. Mukatova, Yekaterina A. Yukhnevich, Dmitriy A. Klyuyev, Andrey I. Yaroshetskiy

“…We calculated mean differences (MD) with 95% confidence intervals (CI), and predictive intervals (PI) using random-effects models. The Cochrane Bias Risk Assessment Tool was applied. …”
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miR-149-3p Is a Potential Prognosis Biomarker and Correlated with Immune Infiltrates in Uterine Corpus Endometrial Carcinoma by Xiaoyuan Lu, Li Jing, Sicong Liu, Haihong Wang, Buze Chen

“…MiR-149-3p may be involved in the occurrence and development of UCEC via pathways including PI3K-Akt signaling pathway, Ras signaling pathway, AGE-RAGE signaling pathway in diabetic complications, focal adhesion, and MAPK signaling pathway. miR-149-3p may inhibit the function of CD8 T cells, cytotoxic cells, eosinophils, iDC, mast cells, neutrophils, NK CD56bright cells, NK CD56dim cells, pDC, T cells, T helper cells, TFH, Th17 cells, and Treg. miR-149-3p was significantly upregulated in UCEC cell lines compared with endometriotic stromal cells. …”
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Temperature-dependent toxicity and mechanisms of florfenicol on the embryonic development of marine medaka (Oryzias melastigma) by Xingying Guo, Xu Gong, Jinhui Wang, Tan-Duc Nguyen, Syed Shabi Ul Hassan Kazmi, Jiezhang Mo, Feng Hua, Wenhua Liu, Zhen Wang

“…For genes related to DNA damage and apoptosis (pi3k, akt, foxo1, tp53, bcl-2, bax, apaf-1, caspase 9, and 3), alterations in gene expression were evident for all florfenicol treatments at both 20 °C and 30 °C. …”
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Absent in melanoma 2: a potent suppressor of retinal pigment epithelial-mesenchymal transition and experimental proliferative vitreoretinopathy by Yu Chen, Mingyuan Jiang, Liping Li, Shanshan Yang, Zuimeng Liu, Shiwen Lin, Wanxiao Wang, Jinyang Li, Feng Chen, Qiang Hou, Xiaoyin Ma, Ling Hou

“…Kyoto Encyclopedia of Genes and Genome analysis revealed that the PI3K-AKT signaling pathway was significantly related to RPE-EMT and that AIM2 inhibited AKT activation in RPE cells by reducing its phosphorylation. …”
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Enhancement of convolutional neural network for urban environment parking space classification by S. Rahman, M. Ramli, F. Arnia, R. Muharar, M. Ikhwan, S. Munzir

“…EfficientParkingNet is suitable for use in parking systems with low computing devices such as the Raspberry Pi because of the small number of parameters.…”
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Organic rare earth feed additive to enhance Penaeus monodon’s muscle quality: A comprehensive insights into texture, amino acid, and fatty acid composition by Jiaoping Yu, Wanqi Wang, Guowei Liao, Zhen Zhang, Yuanyuan Yao, Kaimin Cheng, Jun Hu, Yalin Yang, Zhigang Zhou, Lanfen Fan

“…Additionally, the relative expression levels of muscle growth-related genes PI3K (Phosphoinositide 3-kinase), TOR (target of rapamycin), AKT (Protein kinase B), S6K1 (ribosomal protein S6 kinase 1) and 4E-BP1 (4E-binding protein 1) were significantly higher in shrimp muscle compared to the CON group (P < 0.05). …”
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高浓度雌二醇对刀豆蛋白A诱导小鼠急性自身免疫性肝炎的影响 by 张译文, 汪黎, 邱素苈, 李秋阳, 黎哲, 许成芳

“…利用小鼠肝细胞系AML2，各处理组加入不同浓度的雌二醇，处理24 h和48 h，CCK8法检测各组细胞增殖情况，Annexin V-PI 法检测各组细胞凋亡率。结果苏木素-伊红染色结果显示：分别与Control组和ConA组相比，EE+ConA组肝脏损伤程度加重，且血清ALT和AST水平明显增高，（<italic>P</italic>＜0.000 1； <italic>P</italic>＜0.000 1），差异具有统计学意义。…”
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ABT737通过延迟宫颈癌细胞放射后DNA损伤修复及诱导凋亡而增敏放疗

“…流式细胞术结果显示,单纯放疗组早期凋亡率(Annexin V+,PI-)为23.3%,ABT737联合放疗可以明显提高放射线诱导的细胞凋亡,早期凋亡率最高达50.3%?…”
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Annealing Study on Praseodymium-Doped Indium Zinc Oxide Thin-Film Transistors and Fabrication of Flexible Devices by Zhenyu Wu, Honglong Ning, Han Li, Xiaoqin Wei, Dongxiang Luo, Dong Yuan, Zhihao Liang, Guoping Su, Rihui Yao, Junbiao Peng

“…The flexible devices were prepared using the optimized parameters on the Polyimide (PI) substrate and subjected to static bending tests. …”
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Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model by Zigui Chen, Chunyuan Zhang, Yuhua Fang, He Zhang, Jiawei Luo, Changfeng Miao, Jiale Li, Jun Peng, Yingqi Qiu, Ying Xia, Qisheng Luo

“…Moreover, we also observed elevated phosphorylation of PI3K/AKT and suppressed BAX protein expression. …”
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Effects of high-pressure homogenization on phenolics profile, antioxidant activity, α-glucosidase inhibitory activity, and insulin resistance of peach juice during simulated gastro... by Yijin Peng, Shenke Bie, Shengbao Cai, Linyan Zhou, Chaofan Guo

“…Molecular docking analysis further revealed that key phenolic compounds, including phlorizin and quercetin, interacted effectively with IRS1/PI3K/AKT pathway proteins, contributing to the alleviation of insulin resistance. …”
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外源性人β防御素-2调节子宫内膜异位症细胞增殖/凋亡及炎性因子的表达 by 徐丽南, 王子莲, 陈淑琴

“…【方法】 组织块消化法原代培养EMs细胞,Western Blot法检测EMs细胞hβD-2蛋白、TNF-α及IL-1β的蛋白表达;Western Blot法检测加入重组人hβD-2蛋白(40μg/mL)前后EMs细胞内TNF-α,IL-1β蛋白表达变化;按不同梯度浓度(80、40、20、10 μg/mL)加入人β防御素-2蛋白(hβD-2)后,ELISA法检测EMs细胞培养液上清中TNF-α,IL-1β细胞因子的浓度;加入高剂量hβD-2蛋白(120 μg/mL)并培养EMs细胞24H设为实验组,对照组为完全培养基培养的EMs细胞,MTT法检测实验组及对照组EMs细胞增殖情况;PI法检测实验组及对照组EMs细胞凋亡情况.【结果】 原代培养的EMs细胞可表达hβD-2蛋白、TNF-α、IL-1β蛋白;加入外源性重组人hβD-2蛋白后,EMs细胞表达TNF-α、IL-1β蛋白下调;EMs细胞培养液上清中可检测出TNF-α,IL-1β的表达;在加入hβD-2后,EMs细胞培养液上清内TNF-α、IL-1β表达下降,且随着加入hβD-2浓度增高其下降趋势增大,差异有统计学意义(80>40>20 > 10 μg/mL,P < 0.05);在EMs细胞培养基中加入高浓度外源性人β防御素2蛋白孵育后(120 μg/mL),与对照组相比,EMs细胞的增殖受到明显抑制,且凋亡明显增加(P < 0.05).…”
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Proteomic and serologic assessments of responses to mRNA-1273 and BNT162b2 vaccines in human recipient sera by Thomas E. Hickey, Uma Mudunuri, Heidi A. Hempel, Troy J. Kemp, Nancy V. Roche, Keyur Talsania, Brian A. Sellers, James M. Cherry, Ligia A. Pinto

“…Thirty markers that contributed most to the model were associated with complement cascade and activation; IL-17, TNFR pro-apoptotic, and PI3K signaling; and cell cycle progression.DiscussionThese results demonstrate the utility of proteomics to evaluate correlates or predictors of serological responses to SARS-CoV-2 vaccination.…”
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Evaluation of Non-Invasive Hemoglobin Monitoring in Perioperative Patients: A Retrospective Study of the Rad-67^TM (Masimo) by Philipp Helmer, Andreas Steinisch, Sebastian Hottenrott, Tobias Schlesinger, Michael Sammeth, Patrick Meybohm, Peter Kranke

“…., patient age, skin temperature, sex, perfusion index (PI), and atrial fibrillation, were also analyzed. …”
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去分化联合IDO基因修饰增强人脐带间充质干细胞的抗氧化应激存活及Treg细胞诱导能力 by 匡梅娜, 黄思瑞, 鲁欣, 周畅, 胡耀华, 唐震林, 袁茵

“…通过Western blot检测被感染细胞的IDO蛋白表达，应用流式细胞术测定IDO基因修饰型De-hMSC（IDO/De-hMSC）的免疫表型，同时鉴定其成骨和成脂分化能力。利用Annexin V-FITC/PI双染流式细胞术比较各组细胞在300μmol/Lt-BHP作用下的抗氧化应激生存能力，采用三色荧光标记流式细胞术测定含各组细胞培养上清的条件培养基对正常人外周血单个核细胞（PBMC）中Treg细胞含量的影响。…”
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Five-Year Predictors of Insulin Initiation in People with Type 2 Diabetes under Real-Life Conditions by Sandro Gentile, Felice Strollo, Francesca Viazzi, Giuseppina Russo, Pamela Piscitelli, Antonio Ceriello, Carlo Giorda, Piero Guida, Paola Fioretto, Roberto Pontremoli, Salvatore De Cosmo, the AMD-Annals Study Group

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Platelet-derived extracellular vesicles induced through different activation pathways drive melanoma progression by functional and transcriptional changes by Zeynep Tavukcuoglu, Umar Butt, Alessandra V. S. Faria, Johannes Oesterreicher, Wolfgang Holnthoner, Saara Laitinen, Mari Palviainen, Pia R-M Siljander

“…Distinct tetraspanin signatures of melanoma EVs were induced by all PEV types. While the PI3K-Akt and MAPK signaling pathways were activated by both CRP and FFV PEVs, they differently upregulated the immunomodulatory TGF-β and type-I interferon signaling pathways, respectively. …”
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RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia by Tim Kong, Angelo B. A. Laranjeira, Christopher T. Letson, LaYow Yu, Shuyang Lin, Jared S. Fowles, Daniel A. C. Fisher, Sherwin Ng, Wei Yang, Fan He, Minyoung Youn, Kailen Mark, Ana San Jose, Jingxian Liu, Alexander B. Kim, Maggie J. Cox, Mary C. Fulbright, Aarthi Jayanthan, Gerrit Los, Stacey L. Rentschler, Li Ding, Kathleen M. Sakamoto, Sandra E. Dunn, Grant A. Challen, Stephen T. Oh

“…Supported by mass cytometry (CyTOF) profiling, we reveal aberrant networks of PI3K/AKT/mTOR signalling and NFκB-mediated hyper-inflammation. …”
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Localized COUP‐TFII pDNA Delivery Modulates Stem/Progenitor Cell Differentiation to Enhance Endothelialization and Inhibit Calcification of Decellularized Allografts by Mengmeng Xing, Fei Wang, Ruowen Chu, He Wang, Yuyao Sun, Meng Qian, Huan Jiang, Adam C. Midgley, Guohao Dai, Qiang Zhao

“…COUP‐TFII augmented endothelialization and inhibited calcification in decellularized allografts by modulating the Ang1/Tie2/PI3K/AKT signaling pathway that dictates the fate of Sca‐1+ stem/progenitor cells. …”
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