Bone Mass Loss and Sarcopenia in Ecuadorian Patients by M. Intriago, G. Maldonado, R. Guerrero, O. D. Messina, C. Rios

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Methyl Canthin-6-one-2-carboxylate Inhibits the Activation of the NLRP3 Inflammasome in Synovial Macrophages by Upregulating Nrf2 Expression by Yuanyuan Chen, Zongying Zhang, Yuan Yao, Xiaorong Zhou, Yong Ling, Liming Mao, Zhifeng Gu

“…These alterations were associated with changes in the activation of various signaling pathways, including NF-kB, MAPK, and PI3K-AKT pathways. Notably, pretreatment with Cant significantly reduced LPS/ATP-induced activation of the NLRP3 inflammasome, as evidenced by the decline in the cleaved forms of IL-1β and caspase-1 in cell culture supernatants of BMDMs. …”
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Characterization of phospholipid profiles of egg yolks: Newly classified plasmalogens, distribution of polyunsaturated fatty acids, and the effects of dietary enrichment by Le Cheng, Xinlei Yuan, Ming Zhang, Jianguo Dong, Yao Wu, Rang Wang, Yixuan Li, Lishui Chen, Bing Fang

“…Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), α-Linolenic acid (ALA), and arachidonic acid (ARA) were esterified at sn-1 position of PC and sn-2 position of PE, phosphatidyl inositol (PI) and phosphatidic acid (PA). Microalgae feeding contributed to the deposition of PUFAs at sn-2 position and increased the contents of plasmalogens. …”
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Cardiovascular diseases health literacy among Italian navy personnel: A cross-sectional survey by L. Rondinini, V. Di Fiore, M. Pasquale, G. Cruschelli, D. Rocchi, F. Baglivo, A. Baggiani, A.D. Porretta

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Phenotypic stability of spring bread wheat regenerant lines by V. Yu. Stupko, A. V. Sidorov

“…The yield-based superiority measure (Pi) was also calculated.Results. RLs from cv. ‘Novosibirskaya 15’ were more stable than their donor genotype from the biological viewpoint as well as from the agronomic one. …”
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Methylome profiling of cell-free DNA during the early life course in (un)complicated pregnancies using MeD-seq: Protocol for a cohort study embedded in the prospective Rotterdam pe... by Marjolein M van Vliet, Sam Schoenmakers, Ruben G Boers, Lotte E van der Meeren, Joost Gribnau, Régine P M Steegers-Theunissen

“…A methylation-dependent restriction enzyme (LpnPI) will be used to generate DNA fragments followed by sequencing on the Illumina NextSeq2000 platform. …”
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大鼠脑梗死后缺氧组织显像的动态变化

“…【方法】 利用大鼠大脑中动脉闭塞(MCAO)模型,分别采用99Tcm-HL91放射自显影和EF5免疫荧光的方法进行缺氧显像。分持续缺血组(PI)、1.5 h缺血再灌注组(1.5 h IR)、2 h缺血再灌注组和3 h缺血再灌注组4组,放射自显影的观察时间点选取手术后1 d和14 d,免疫荧光则选取术后1、3、7、14、21 d,每个时间点各3只大鼠。…”
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硫化氢对化学性缺氧诱导PC12细胞凋亡的影响

“…【目的】 探讨硫化氢(H2S)对抗二氯化钴(CoCl2)诱导PC12细胞凋亡的作用及其初步机制65377;【方法】 应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠(NaHS)作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶(PI)染色流式细胞技术(FCM)检测细胞凋亡率; Hoechst33258 染色检测细胞凋亡的形态学变化; 罗丹明123(Rh123) 染色荧光显微镜照相检测细胞线粒体膜电位(MMP); 2’,7’-二氯荧光黄双乙酸盐(DCFH-DA)染色荧光显微镜照相检测细胞内的活性氧 (ROS)水平65377;【结果】 CoCl2引起PC12细胞的存活率显著降低及凋亡率明显增加, 同时引起PC12细胞的MMP 明显降低及ROS 生成显著增加65377;在600 μmol/L CoCl2处理PC12细胞前,应用100 ~ 400 μmol/L NaHS 预处理30 min,呈浓度依赖性地阻断CoCl2引起PC12细胞的存活率降低; 200 μmol/L65380;400 μmol/L NaHS 预处理能显著地降低600 μmol/L CoCl2 引起PC12 细胞的凋亡率增加并阻断CoCl2 引起的MMP降低及ROS升高65377;【结论】 H2S 具有神经细胞保护作用, 能明显地对抗CoCl2诱导的PC12细胞凋亡,此作用可能与其减少ROS生成,提高MMP有关65377;…”
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Intratumor microbiome-derived butyrate promotes chemo-resistance in colorectal cancer by Linsheng Xu, Linsheng Xu, Bingde Hu, Jingli He, Xin Fu, Na Liu

“…Mechanistically, this resistance was attributed to butyrate’s activation of the PI3K-AKT signaling pathway.ConclusionOur results suggest that intratumor microbiome-derived butyrate contributes to chemoresistance in colorectal cancer, highlighting the potential prognostic and therapeutic significance of the intratumor microbiome.…”
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Discapacidad física, personalidad y competencias laborales. Estudio comparativo con población normativa by Sylvia Sylvia Lara Paredes, Marta Alzina Carreró, Begoña Mariné Basterra, Ariana Sabaté García

“…Los instrumentos estandarizados empleados para el estudio fueron el Inventario de Personalidad (NEO-PI-R) y el Cuestionario SOSIA para la Gestión por Competencias. …”
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A CROSS-SECTIONAL STUDY ASSESSING THE ASSOCIATION BETWEEN PERIODONTAL DISEASE AND SOCIO-BEHAVIORAL RISK FACTORS by Oana Chipirliu, Marian Viorel Crăciun, Mădălina Nicoleta Matei

“…To assess local hygiene and inflammation status, in this study we used the O’Leary plaque index (PI) and the Silness and Löe gingival index (GI). …”
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Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways by Liang Zhu, Yanchi Bi, Ting Liang, Po Zhang, Xiao Xiao, Tengbo Yu

“…Results Network pharmacology has revealed that GK regulates OA via several key pathways, especially NF-κB, HIF-1, PI3K-AKT, and substances like reactive oxygen species. …”
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To Compare and Evaluate the Efficacy of 1% Alendronate Gel and 10% Doxycycline Gel as an Adjunct to SRP in Patients with Chronic Periodontitis: A Randomized Controlled Clinical Tri... by Laloo Phassang, Radhika Kapoor, Prashant Tyagi, Amit Wadhawan

“…Changes in gingival index (GI), plaque index (PI), probing pocket depth (PPD), and relative attachment level (RAL) were measured across three groups: a control group (Group C) and two experimental groups (Group A and B). …”
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Gene expression analysis reveals mir-29 as a linker regulatory molecule among rheumatoid arthritis, inflammatory bowel disease, and dementia: Insights from systems biology approach... by Devi Soorya Narayana S, Vino Sundararajan

“…These hub genes were mostly enriched in significant processes and pathways like tissue development, collagen binding, cell adhesion, regulation of ERK1/2 cascade, PI3K-AKT signaling, and cell surface receptor signaling. …”
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Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…The apoptosis of multiple myeloma cells was studied using AO/EB and Annexin V-FITC/PI staining methods. Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. …”
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氧化苏木素诱导人乳癌MCF-7细胞凋亡及其作用机制

“…【目的】 探讨氧化苏木素对人乳癌MCF-7细胞的凋亡作用及其相关作用机制 【方法】 MTT实验检测氧化苏木素对MCF-7细胞和正常乳腺上皮细胞MCF 10A的细胞毒作用;Annexin V/PI双染,流式细胞仪检测细胞凋亡的发生;荧光染料DiOC6染色,流式细胞仪检测细胞内线粒体膜电位的变化;Western blot实验检测细胞浆细胞色素C及细胞内caspase-9和survivin蛋白的变化 【结果】 氧化苏木素对人乳癌MCF-7细胞具有高效的细胞毒作用,而对正常乳腺上皮MCF 10A细胞毒性较弱,IC50值分别为(7.15 ± 0.43)μmol/L 和(33.56 ± 2.87) μmol/L;07.51530 μmol/L的氧化苏木素处理MCF-7细胞48 h后,细胞的凋亡率从(3.37 ± 0.66)%依次升高到(16.8 ± 1.27)%(31.31 ± 4.22)%(51.23 ± 5.55)%;DiOC6染色流式细胞仪检测结果显示氧化苏木素可以剂量依赖性地降低线粒体膜电位;Western blot结果显示氧化苏木素诱发了线粒体内细胞色素C的释放和细胞内caspase-9蛋白的剪切激活,同时氧化苏木素也可剂量依赖性地降低survivin蛋白的表达 【结论】 氧化苏木素可通过线粒体通路诱导MCF-7细胞凋亡,下调survivin蛋白可能是其诱导细胞凋亡的主要机制…”
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Oncogenic RIT1 mutations confer ferroptosis vulnerability in lung adenocarcinoma by Ruilan Ma, Dian Yang, Peng Wang, Ziyi Zhang, Xuehong Zhang, Jialiang Song, Han Liu, Shuyan Liu, Yingqiu Zhang, Lijuan Zou

“…Transcriptomic profiling indicated that oncogenic RIT1 mutant expression influenced common tumorigenic RAS/MAPK, PI3K/AKT, and E2F1 pathways, in addition to altered NFE2L2 target expression. …”
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Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis by Zhi-Bo Zhang, Zheng Shi, Lan-Fang Yang, Hong-Bin Gao

“…SMMC7721 cell proliferation, cycle, apoptosis, and invasiveness were detected by MTT, flow cytometry, Annexin V-FITC/PI, and invasion assay, respectively. Phospho-eNOS levels in human umbilical vein endothelial cells (HUVECs) cocultured with SMMC7721 cell supernatants were analyzed by Western blot. …”
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mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer by E. D. Kulaeva, E. S. Muzlaeva, E. V. Mashkina

“…The search for common genes among the most strongly differentially expressed genes among all comparison groups resulted in the identification of an expression signature consisting of the CCL20, CDKN2A, CTCFL, piR-55219, TRH, SLC27A6 and EPHA5 genes. The transcription level of the CCL20 and CDKN2A genes becomes increased at the stage of neoplastic epithelial changes and stays so in cervical cancer. …”
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Efficacy of osmotic laxative polyethylene glycol 4000 in treatment of constipation in pediatrics by S. I. Erdes, B. O. Matsukatova, S. A. Revyakina, Ye. V. Casanave

“…Dynamics of PEGEG parameters, namely electrical activity (Pi/Рs) of every region of GIT before treatment, quotient of various gut regions interrelation, and rhythmicity quotient (Kritm) in general, proved presence of concomitant pathology of the upper parts of GIT, severe dysfunctional disorders. …”
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