True cancer stem cells exhibit relative degrees of dormancy and genomic stability by Sanford H. Barsky, Krista Mcphail, Justin Wang, Jordan Dillard, Crystal J. Beard, Yin Ye

“…Methods: Using a human PDX, Mary-X, that overall expressed a strong cancer stem cell phenotype, the study conducted both GPP-labelled retroviral transfection and fluorescent microsphere uptake studies to distinguish proliferating from dormant cells and array CGH to identify regions of amplifications (gains) and deletions (losses) on the overall Mary-X population and then applied derived probes by FISH on individual cells to identify a genomically stable subpopulation. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…Efferocytosis promoted macrophage polarization from the M1 to the M2 phenotype, reduced the expression of proinflammatory cytokines, and decreased the levels of inflammation and oxidative stress. …”
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A Rare Case of Severe Congenital RYR1-Associated Myopathy by Nicola Laforgia, Manuela Capozza, Lucrezia De Cosmo, Antonio Di Mauro, Maria Elisabetta Baldassarre, Francesca Mercadante, Anna Laura Torella, Vincenzo Nigro, Nicoletta Resta

“…We report clinical and genetic characteristics of a male preterm newborn, whose phenotype was characterized by severe hypotonia and hyporeactivity, serious respiratory distress syndrome that required mechanical ventilation, clubfoot, and other dysmorphic features. …”
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Molecular biological research and molecular homologous modeling of Bw.03 subgroup by WANG Li, KONG Yongkui, JIN Huifang, LIU Xin, XIE Ying, LIU Xue, CHANG Yanli, WANG Yafang, YANG Shumiao, ZHU Di, YANG Qiankun

“…[Objective] To study the molecular biological mechanism for a case of ABO blood group B subtype, and perform three-dimensional modeling of the mutant enzyme. [Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence-specific primer polymerase chain reaction (PCR-SSP), and the exon 6 and 7 of the ABO gene were sequenced and analyzed. …”
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Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion by Mehul Vora, Jonathan Dietz, Zachary Wing, Karen George, Jun Kelly Liu, Christopher Rongo, Cathy Savage-Dunn

“…Mutations in sma-9 partially suppress the small body size phenotype of sma-3, suggesting some level of antagonism between these factors and challenging the prevailing model for Schnurri function. …”
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Karyopherin Alpha 2-Expressing Pancreatic Duct Glands and Intra-Islet Ducts in Aged Diabetic C414A-Mutant-CRY1 Transgenic Mice by Satoshi Okano, Akira Yasui, Shin-ichiro Kanno, Kennichi Satoh, Masahiko Igarashi, Osamu Nakajima

“…Our earlier studies demonstrated that cysteine414- (zinc-binding site of mCRY1-) alanine mutant mCRY1 transgenic mice (Tg mice) exhibit diabetes characterized by the reduction of β-cell proliferation and by β-cell dysfunction, presumably caused by senescence-associated secretory phenotype- (SASP-) like characters of islets. Earlier studies also showed that atypical duct-like structures in the pancreas developed age-dependently in Tg mice. …”
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Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus by Paola del Carmen Guerra-de Blas, Yael Sebastián Villaseñor-Talavera, Daniela de Jesús Cruz-González, Lourdes Baranda, Lesly Doníz-Padilla, Carlos Abud-Mendoza, Roberto González-Amaro, Adriana Elizabeth Monsiváis-Urenda

“…Although the inhibitory receptor ILT4/LILRB2 has been related with the tolerogenic phenotype of DC, the possible role of this receptor in the breakdown of DC tolerogenic function in systemic lupus erythematosus (SLE) has not been elucidated. …”
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Astrocyte-Derived Interleukin 11 Modulates Astrocyte–Microglia Crosstalk via Nuclear Factor-κB Signaling Pathway in Sepsis-Associated Encephalopathy by Dandan Zhu, Peng Wang, Xiyue Chen, Kaituo Wang, Yunsong Wu, Min Zhang, Jianhua Qin

“…Cytokine array analysis revealed that less interleukin 11 (IL11) was secreted by astrocytes following LPS treatment, which further promoted reprogramming of microglia to pro-inflammatory M1 phenotype via the nuclear factor-κB (NF-κB) signaling pathway. …”
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Adipose Tissue-Derived Stem Cells Differentiate into Carcinoma-Associated Fibroblast-Like Cells under the Influence of Tumor-Derived Factors by Constantin Jotzu, Eckhard Alt, Gabriel Welte, Jie Li, Bryan T. Hennessy, Eswaran Devarajan, Srinivasalu Krishnappa, Severin Pinilla, Lilly Droll, Yao-Hua Song

“…We demonstrated that a significant percentage of hASCs differentiated into a CAF-like myofibroblastic phenotype (e.g., expression of alpha smooth muscle actin and tenascin-C) when exposed to conditioned medium from the human breast cancer lines MDAMB231 and MCF7. …”
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Characteristic and Antimicrobial Resistance of Bacillus cereus Group Isolated from Food in Poland by Joanna Kowalska, Elżbieta Maćkiw, Dorota Korsak, Jacek Postupolski

“…Ampicillin and penicillin resistance was the most common resistance phenotype and was identified in 100% of the B. cereus isolates. …”
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Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa by Hongyao Mei, Jiajun Yan, Xuexin Jia, Weilin Wang, Shuangshuang Li, Ruiqi Sun, Hongjiao Jiang, Lijun Xie, Chuanen Zhou, Shiqie Bai, Lu Han

“…In this study, to elucidate the mechanism of compound leaf development, we isolated and examined the alfalfa phenotype Chuancao No.7, exhibiting five leaflets. …”
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Refining prognostic assessment of diffuse large B-cell lymphoma: insights from multi-omics and single-cell analysis unveil SRM as a key target for regulating immunotherapy by Xiaojie Liang, Jia Guo, Baiwei Luo, Weixiang Lu, Qiumin Chen, Yeling Deng, Yunong Yang, Liang Wang

“…HPSI shared malignant proliferative phenotype resembling Burkitt lymphoma. Genomic analysis revealed extensive copy-number loss in the chemokine and interleukin coding regions within HPSI. …”
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Reactivation of Denervated Schwann Cells by Embryonic Spinal Cord Neurons to Promote Axon Regeneration and Remyelination by Xinyu Fang, Chaofan Zhang, Chongjing Zhang, Yuanqing Cai, Zibo Yu, Zida Huang, Wenbo Li, Wenming Zhang

“…After excision of the transplanted cells, the SCs returned to their dedifferentiated phenotype and upregulated growth-associated gene expression. …”
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Endometrial factors in the implantation failure spectrum: protocol of a MUltidisciplinary observational cohort study in women with Repeated Implantation failure and recurrent Misca... by Servaas Morré, Ron van Golde, Andrea Romano, Linda Stevens Brentjens, Denise Habets, Janneke Den Hartog, Salwan Al-Nasiry, Lotte Wieten, Aafke Van Montfoort

“…The study parameters are the steroid profile (steroid levels and mRNA levels, protein expression and activity of steroid enzymes) in endometrial tissue and peripheral blood, as well as the activating or inhibitory phenotype of uNK cells based on receptor expression in menstrual blood and endometrial tissue and determination of the vaginal and endometrial microbiome using the inter spacer bacterial profiling technique.Ethics and dissemination The protocol is approved by the local medical ethical review committee at the Maastricht University Medical Centre. …”
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Insecticide resistant Anopheles from Ethiopia but not Burkina Faso show a microbiota composition shift upon insecticide exposure by Netsanet Worku, Antoine Sanou, Juliane Hartke, Marion Morris, Fatoumata Cissé, Salimata Ouédraogo, Madou Tapsoba, Nicola Vallon, Tewodros Debebe Akilu, Ligabaw Worku, Moussa Wamdaogo Guelbeogo, Victoria A. Ingham

“…Understanding the underlying mechanisms contributing to this phenotype is necessary to both track the spread of resistance and to design new tools to overcome it. …”
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A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…Conclusion This study identified a novel likely pathogenic GABRA2 extracellular domain variant in a Chinese family causing the DEE phenotype. The results expand the genotypic and phenotypic spectrum of GABRA2‐related DEE.…”
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Magnetic resonance imaging does not distinguish Kallmann syndrome from normosmic isolated hypogonadotropic hypogonadism by Małgorzata Kałużna, Katarzyna Katulska, Katarzyna Ziemnicka, Pola Kompf, Bartłomiej Budny, Paweł Komarnicki, Michał Rabijewski, Jerzy Moczko, Jarosław Kałużny, Marek Ruchała

“…We aimed to correlate findings of magnetic resonance imaging (MRI) of the pituitary gland and olfactory system in IHH patients with the patients’ olfactory phenotype. Patients and methods: The present research was a single-center retrospective case–control study. …”
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Bioinformatic identification of important roles of COL1A1 and TNFRSF12A in cartilage injury and osteoporosis by Muzi Liu, Shiguo Gong, Xin Sheng, Zihong Zhang, Xichun Wang

“…Through WGCNA, we identified gene modules that were closely associated with disease phenotypes and then screened for key genes that intersected with differentially expressed genes. …”
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Epithelial cell diversity and immune remodeling in bladder cancer progression: insights from single-cell transcriptomics by Jianpeng Li, Yunzhong Jiang, Minghai Ma, Lu Wang, Minxuan Jing, Zezhong Yang, Mengzhao Zhang, Ke Chen, Jinhai Fan

“…Macrophages polarized toward a pro-tumorigenic phenotype, while VEGFA + mast cells promoted angiogenesis in early-stage BC, suggesting their role as potential targets for therapeutic intervention in NMIBC. …”
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Association between P2X7 Receptor Polymorphisms and Bone Status in Mice by Susanne Syberg, Peter Schwarz, Solveig Petersen, Thomas H. Steinberg, Jens-Erik Beck Jensen, Jenni Teilmann, Niklas Rye Jørgensen

“…Because P2X7 receptors are expressed in bone cells and are implicated in bone physiology, we asked whether strains with the P451L mutation have a different bone phenotype. By sequencing the most common strains of inbred mice, we found that only a few strains (BALB, NOD, NZW, and 129) were harboring the wild allelic version of the mutation (P451) in the gene for the purinergic receptor P2X7. …”
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