Exploring the therapeutic effect of human recombinant IL11 on lesioned OA human osteochondral explants by Margo Tuerlings, Evelyn Houtman, Elisa J.H. Muusers, Janneke Simon, Maurice W. de Haan, Ilja Boone, Yolande F.M. Ramos, Rachid Mahdad, Ingrid Meulenbelt

“…Upon treatment of ex vivo OA articular cartilage with hrIL11, we overall observed unbeneficial effects on chondrocyte phenotype, as illustrated by upregulation of MMP13, EPAS1, RUNX2, and POSTN. …”
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Culture dependent and independent approaches reveal the role of specific bacteria in human skin aging by Jing‐jing Xia, Qian Zhong, Zhi‐ming Li, Qing‐zhen Wei, Liu‐yi‐qi Jiang, Cheng Duan, Hui‐jue Jia, Yi‐mei Tan, Lian‐yi Han, Jean Krutmann, Jiucun Wang, Xiao Liu

“…Abstract Skin aging is a dynamic process involving a spectrum of phenotypic changes, making it an attractive model for studying microbiome‐phenotype interactions. …”
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Phenology analysis for trait prediction using UAVs in a MAGIC rice population with different transplanting protocols by Shoji Taniguchi, Toshihiro Sakamoto, Haruki Nakamura, Yasunori Nonoue, Di Guan, Akari Fukuda, Hirofumi Fukuda, Kaede C. Wada, Takuro Ishii, Jun-Ichi Yonemaru, Daisuke Ogawa

“…However, whether CH parameters are applicable to other rice populations and to different cultivation methods, and whether vegetation indices such as the chlorophyll index green (CIg) can function for phenotype prediction remain to be elucidated. Here we show that CH and CIg exhibit different patterns with different cultivation protocols, and each has its own character for the prediction of rice phenotypes. …”
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ERG mediates the differentiation of hepatic progenitor cells towards immunosuppressive PDGFRα+ cancer-associated fibroblasts during hepatocarcinogenesis by Haoran Bai, Xinyu Zhu, Lu Gao, Shiyao Feng, Hegen Li, Xiaoqiang Gu, Jiahua Xu, Chen Zong, Xiaojuan Hou, Xue Yang, Jinghua Jiang, Qiudong Zhao, Lixin Wei, Li Zhang, Zhipeng Han, Wenting Liu, Jianxin Qian

“…On the other hand, they produce TGFβ, inducing the polarization of these macrophages towards an immunosuppressive phenotype. According to the in vitro and in vivo results, hepatic progenitor cells (HPCs) can aberrantly differentiate into PDGFRα+ CAFs upon stimulation with inflammatory cytokine. …”
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Exploiting Cancer Dormancy Signaling Mechanisms in Epithelial Ovarian Cancer Through Spheroid and Organoid Analysis by Emily J. Tomas, Yudith Ramos Valdes, Jennifer Davis, Bart Kolendowski, Adrian Buensuceso, Gabriel E. DiMattia, Trevor G. Shepherd

“…Our results indicated that AMP-activated protein kinase (AMPK) activity was significantly upregulated and protein kinase B (Akt) was downregulated in EOC spheroids compared to organoids, suggesting a clear differential phenotype. Through RNA sequencing analysis, we further supported these phenotypic differences and highlighted the significance of cell cycle regulation in organoids. …”
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Genotypic Spectrum in a Cohort of Sri Lankan Patients With Homocystinuria by Hewa Warawitage Dilanthi, Kandana Liyanage Subhashinie Jayasena, Nambage Dona Priyani Dhammika, Neluwa Liyanage Ruwan Indika, Matara Mahavidanage Nishani De Silva, Imalke Kankananarachchi, Pushpa Malkanthi Gardiye Punchihewa, Dharma Irugalbandara, Sabine Schroeder, Kosala Karunaratne, Eresha Jasinge

“…Spectrum of genetic variants in CBS gene and their correlation with the phenotypes of homocystinuria in Sri Lankan patients have not been reported to date. …”
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Isolation and Characterization of an Adult Stem Cell Population from Human Epidural Fat by Nedaa Al-Jezani, Roger Cho, Anand O. Masson, Brian Lenehan, Roman Krawetz, Frank G. Lyons

“…Furthermore, all patient lines demonstrated collagen contraction capacity, suggesting either a contaminating activated fibroblast population or HEF MSCs/progenitors also demonstrating a fibroblast-like phenotype. In vivo analysis suggests that these cell populations may contribute to the dura. …”
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Xenopus laevis neural stem progenitor cells exhibit a transient metabolic shift toward glycolysis during spinal cord regeneration by Paula G. Slater, Paula G. Slater, Miguel E. Domínguez-Romero, Guillermo Campos, Guillermo Campos, Vania Aravena, Vania Aravena, Javier Cavieres-Lepe, Verónica Eisner

“…Furthermore, mitochondria adopted a swollen phenotype, which did not impair mitochondrial function or cellular energy balance. …”
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Prognostic Value of N1/N2 Neutrophils Heterogeneity and Tertiary Lymphoid Structure in Hepatocellular Carcinoma Patients by Yanfei Lang, Weiwei Fu, Wei Xu, Chao Ma, Xiuyun Tian, Chunyi Hao, Shigang Ding

“…Tumor‐associated neutrophils exhibit plasticity, adopting either an antitumorigenic N1 (MPO+ CD206−) or a pro‐tumorigenic N2 (MPO+ CD206+) phenotype. We explored the prognostic value of neutrophil plasticity and TLS maturity in HCC in both tumor and peritumoral tissues and addressed their interaction. …”
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Circulating Interleukin-6 (but Not Other Immune Mediators) Associates with Criteria for Fried’s Frailty among Very Old Adults by Gilberto Santos Morais Junior, Diego Ignacio Valenzuela Perez, Audrey Cecília Tonet-Furioso, Lucy Gomes, Karla Helena Coelho Vilaça, Vicente Paulo Alves, Clayton Franco Moraes, Otávio Toledo Nóbrega

“…The present research aimed to investigate the association of components of Fried’s frailty (as well as of the phenotype as a whole) with total serum levels of a panel of inflammatory mediators. …”
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Clinical characteristics and outcomes in Asian patients with heart failure with mildly reduced ejection fraction by Julian Cheong Kiat Tay, Shaw Yang Chia, Siew Hui Michelle Koh, David Kheng Leng Sim, Ping Chai, Seet Yoong Loh, Fazlur Rehman Jaufeerally, Sheldon Shao Guang Lee, Patrick Zhan Yun Lim, Jonathan Yap

“…HFmrEF represents a distinct HF phenotype with high atherosclerotic burden and clinical outcomes saddled in between those of HFrEF and HFpEF. …”
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A case of 49,XXXYY followed-up from infancy to adulthood with review of literature by Junko Kanno, Akinobu Miura, Sayaka Kawashima, Hirohito Shima, Dai Suzuki, Miki Kamimura, Ikuma Fujiwara, Masayuki Kamimura, Mitsugu Uematsu, Masataka Kudo, Atsuo Kikuchi

“…This report will contribute to an enhanced understanding of the 49,XXXYY phenotype, aiding in the diagnosis, treatment, and genetic counseling of future cases.…”
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Neuroprotective and Antiapoptotic Activity of Lineage-Negative Bone Marrow Cells after Intravitreal Injection in a Mouse Model of Acute Retinal Injury by Anna Machalińska, Dorota Rogińska, Ewa Pius-Sadowska, Miłosz P. Kawa, Edyta Paczkowska, Michał Rudnicki, Renata Lejkowska, Bartłomiej Baumert, Barbara Wiszniewska, Bogusław Machaliński

“…Furthermore, transplanted Lin−BMCs differentiated locally into cells with a macrophage-like phenotype. Finally, Lin−BMCs treatment was associated with generation of two distinct transcriptomic patterns. …”
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Nuclear farnesoid X receptor protects against bone loss by driving osteoblast differentiation through stabilizing RUNX2 by Qi Dong, Haoyuan Fu, Wenxiao Li, Xinyu Ji, Yingchao Yin, Yiran Zhang, Yanbo Zhu, Guoqiang Li, Huiyang Jia, Heng Zhang, Haofei Wang, Jinglue Hu, Ganggang Wang, Zhihao Wu, Yingze Zhang, Sujuan Xu, Zhiyong Hou

“…In this study, in vitro and in vivo models revealed delayed bone development and an osteoporosis phenotype in mice lacking FXR in bone marrow mesenchymal stem cells (BMSCs) and osteoblasts due to impaired osteoblast differentiation. …”
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Prospective Longitudinal Characterization of the Relationship between Diabetes and Cardiac Structural and Functional Changes by Amrit Chowdhary, Nicholas Jex, Sharmaine Thirunavukarasu, Amanda MacCannell, Natalie Haywood, Altaf Almutairi, Lavanya Athithan, Manali Jain, Thomas Craven, Arka Das, Noor Sharrack, Christopher E. D. Saunderson, Anshuman Sengupta, Lee Roberts, Peter Swoboda, Richard Cubbon, Klaus Witte, John Greenwood, Sven Plein, Eylem Levelt

“…The long-term impact of T2D on cardiac phenotype in the absence of cardiovascular disease and other clinical events is unknown. …”
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Stemness-related gene signatures as a predictive tool for breast cancer radiosensitivity by Jinzhi Lai, Rongfu Huang, Jingshan Huang

“…Comparative clonogenic and CCK8 assays demonstrated a radioresistant phenotype in the MCF-7/IR compared to MCF-7. In vitro studies utilizing both the MCF-7/IR and MCF-7 cell lines validated the expression of two radiosensitivity genes.ConclusionThis study identified a stemness-related gene signature predictive of radiosensitivity in breast cancer. …”
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Long-read sequencing revealed complex biallelic pentanucleotide repeat expansions in RFC1-related Parkinson’s disease by Peng Liu, Fan Zhang, Xinhui Chen, Xiaosheng Zheng, Miao Chen, Zhiru Lin, Shuqi Chen, Lebo Wang, Xinchen Wang, Nan Jin, Chenxin Ying, Fei Xie, Bo Wang, Sheng Wu, Zhidong Cen, Wei Luo

“…However, the genotype-phenotype correlation and underlying mechanism are mostly unknown. …”
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Development and Validation of a Machine Learning Method Using Vocal Biomarkers for Identifying Frailty in Community-Dwelling Older Adults: Cross-Sectional Study by Taehwan Kim, Jung-Yeon Choi, Myung Jin Ko, Kwang-il Kim

“… Abstract BackgroundThe two most commonly used methods to identify frailty are the frailty phenotype and the frailty index. However, both methods have limitations in clinical application. …”
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Effectiveness of dupilumab treatment against refractory eosinophilic chronic rhinosinusitis by Daiki Nakashima, MD, Tsuguhisa Nakayama, MD, PhD, Shunsuke Minagawa, MD, PhD, Tetsuya Adachi, MD, PhD, Chieko Mitsuyama, MD, Yoko Shida, MD, Tsuneya Nakajima, MD, PhD, Shin-ichi Haruna, MD, PhD, Yoshinori Matsuwaki, MD, PhD

“…Few studies that reported the effect of dupilumab on CRSwNP focused on a single phenotype of CRSwNP, such as ECRS. Objectives: This study aimed to determine the effectiveness of dupilumab in ECRS with postoperative recurrence. …”
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Identification of prognostic biomarker of non-small cell lung cancer based on mitochondrial permeability transition-driven necrosis-related genes and determination of anti-tumor ef... by Zhifei Ma, Wen Chen, Aiping Zhang, Xiaokang Shen, Lin Zheng

“…Finally, ARL14 knockdown inhibited the malignant phenotype of NSCLC cells. Conclusion We first performed the comprehensive analysis of MPTDN in NSCLC and screened three NSCLC-related biomarkers as promising biomarkers. …”
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