The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis by Louise Tzung-Harn Hsieh, Belinda S Hall, Jane Newcombe, Tom A Mendum, Sonia Santana Varela, Yagnesh Umrania, Michael J Deery, Wei Q Shi, Josué Diaz-Delgado, Francisco J Salguero, Rachel E Simmonds

“…Loss of the glycocalyx is likely to be of particular mechanistic importance, since knockdown of galactosyltransferase II (beta-1,3-galactotransferase 6; B3GALT6), the GAG linker-building enzyme, phenocopied the permeability and phenotypic changes induced by mycolactone. Additionally, mycolactone depleted many secreted basement membrane components and microvascular basement membranes were disrupted in vivo during M. ulcerans infection in the mouse model. …”
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Second-line drug-resistant TB and associated risk factors in Karakalpakstan, Uzbekistan by S. Moe, I. Azamat, S. Allamuratova, M. Oluya, A. Khristusev, M.L. Rekart, K. Mamitova, G. Bidwell, C. Gomez-Restrepo, B. Kalmuratov, Z. Tigay, N. Parpieva, K. Safaev, N. Sitali, D. Gomez, A. Mikhail, A. Sinha

“…We assessed second-line drug-resistant TB (SLDR-TB) rates using phenotypic drug susceptibility testing (pDST). Demographic and clinical characteristics associated with SLDR-TB were analysed using multivariable logistic regression models based on the Allen-Cady approach. …”
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Treating Metabolic Dysregulation and Senescence by Caloric Restriction: Killing Two Birds with One Stone? by Lara Russo, Serena Babboni, Maria Grazia Andreassi, Jalil Daher, Paola Canale, Serena Del Turco, Giuseppina Basta

“…Cellular senescence is a state of permanent cell cycle arrest accompanied by metabolic activity and characteristic phenotypic changes. This process is crucial for developing age-related diseases, where excessive calorie intake accelerates metabolic dysfunction and aging. …”
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Tumor organoids in cancer medicine: from model systems to natural compound screening by Rong Cong, Can Lu, Xinying Li, Zhijie Xu, Yaqin Wang, Shusen Sun

“…These self-assembled 3D cell clusters closely replicate the histopathological, genetic, and phenotypic characteristics of primary tissues, making them invaluable tools in cancer research and drug screening.Objective This review addresses the challenges in developing in vitro models that accurately reflect tumor heterogeneity and explores the application of tumor organoids in cancer research, with a specific focus on the screening of natural products for antitumor therapies.Methods This review synthesizes information from major databases, including Chemical Abstracts, Medicinal and Aromatic Plants Abstracts, ScienceDirect, Google Scholar, Scopus, PubMed and Springer Link. …”
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Divergence in the Morphology and Energy Metabolism of Adult Polyphenism in the Cowpea Beetle <i>Callosobruchus maculatus</i> by Zhong Du, Xiaokun Liu, Sipei Liu, Lei Jiang, Le Zong, Wenjie Li, Weili Fan, Lijie Zhang, Fengming Wu, Siqin Ge

“…Adult polyphenism is a prevalent form of adaptive evolution that enables insects to generate discrete phenotypes based on environmental factors. However, the morphology and molecular mechanisms underlying adult dimorphism in <i>Callosobruchus maculatus</i> (a global storage pest) remain elusive. …”
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Mapping hippocampal glutamate in healthy aging with in vivo glutamate-weighted CEST (GluCEST) imaging by Maggie K. Pecsok, Heather Robinson, Ally Atkins, Monica E. Calkins, Monica E. Calkins, Mark A. Elliott, Arianna Mordy, Jacquelyn Stifelman, Ruben C. Gur, Ruben C. Gur, Paul J. Moberg, Ravi Prakash Reddy Nanga, Kosha Ruparel, Kosha Ruparel, Russell T. Shinohara, David A. Wolk, Ravinder Reddy, David R. Roalf, David R. Roalf

“…These findings offer a step toward elucidating the asymmetrical trajectory of hippocampal glutamatergic alterations and their relationship to cognitive phenotypes.…”
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Stabilized β-Catenin Ameliorates ALPS-Like Symptoms of B6/lpr Mice by Xiaoxie Xu, Jun Huang, Mei Zhao, Huanpeng Chen, Jinhua Mo, Xiaoqing Zhou, Qiao Su, Bolan Yu, Zhaofeng Huang

“…In the present study, we introduced β-catTg into lpr/lpr mice and aimed to explore the potential role of stabilized β-catenin (β-catTg) in the development of ALPS-like phenotypes of lpr/lpr mice. We found that the total splenocyte cells and some compositions were slightly downregulated in β-catTglpr/lpr mice, especially the CD4 and CD8 TEM cells were significantly reduced. …”
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The mutational landscape of Staphylococcus aureus during colonisation by Francesc Coll, Beth Blane, Katherine L. Bellis, Marta Matuszewska, Toska Wonfor, Dorota Jamrozy, Michelle S. Toleman, Joan A. Geoghegan, Julian Parkhill, Ruth C. Massey, Sharon J. Peacock, Ewan M. Harrison

“…We demonstrated the phenotypic effect of multiple adaptive mutations in vitro, including changes in haemolytic activity, antibiotic susceptibility, and metabolite utilisation. …”
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Spatial Distribution of Genetic, Ploidy, and Morphological Variation of the Edaphic Steno-Endemic <i>Alyssum moellendorfianum</i> (Brassicaceae) from the Western Balkans by Jasna Hanjalić Kurtović, Belma Kalamujić Stroil, Sonja Siljak-Yakovlev, Naris Pojskić, Adaleta Durmić-Pašić, Alma Hajrudinović-Bogunić, Lejla Lasić, Lejla Ušanović, Faruk Bogunić

“…Polyploidy is a powerful mechanism driving genetic, physiological, and phenotypic changes among cytotypes of the same species across both large and small geographic scales. …”
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Domestication of medicinal plants (Lonicera japonica Thunb.) in China: comparison of morphological, resistance and biochemical traits between wild and cultivated populations by Congzhe Hou, Luyao Huang, Zhuangzhuang Li, Nan Sun, Sheng Yang, Jia Li, Zhenhua Liu

“…However, in terms of resistance against powdery mildew, wild individuals displayed evident advantages over their cultivated counterparts.ConclusionThe observed phenotypic variation within the honeysuckle field provides empirical support for the hypothesis that farmer management practices influence domestication syndrome, as indicated by the deliberate enhancement of specific desirable traits during species domestication.…”
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Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans by Shakun Karki, Melissa G. Farb, Samantha Myers, Caroline Apovian, Donald T. Hess, Noyan Gokce

“…Functional alterations in lipolysis in human adipose tissue may play a role in shaping cardiometabolic phenotypes in human obesity.…”
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Impact of ABO gene polymorphism and von Willebrand factor on genetic susceptibility to acute lymphoblastic leukemia in Egyptian pediatric patients by Elsayed I. Salim, Ahmed N. Shaaban, Amal K. Seleem, Zeinab I. Attia

“…Methods Seventy-two ALL patients and 36 healthy controls were ABO phenotyped and genotyped for the ABO rs2519093 polymorphism using PCR–RFLP. …”
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ANALYSIS OF CHANGES IN AFRICAN SWINE FEVER VIRUS GENETIC STRUCTURE AND BIOLOGICAL PROPERTIES DURING ADAPTATION TO CONTINUOUS CELL CULTURE by Ali Mazloum, N. G. Zinyakov, A. S. Pershin, I. V. Shevchenko, I. Yu. Zhukov, D. N. Fedoseyeva, D. V. Sharypova, A. S. Igolkin, N. N. Vlasova

“…Comparative analysis of the virus biological characteristics and detection of differences in its genome structure affecting certain phenotypic features is a main method used for the virus basic pathogenicity and immunogenicity examination. …”
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Age and moisture affect the relationship between competition and tree growth by Xinyu Han, Lushuang Gao, Mingqian Liu, Yihong Zhu, Keda Cui, Xinyu Zhang, Xiuhai Zhao, Ruibo Zhang, Klaus von Gadow, Yiyu Tian, Kangchen Wang

“…Our findings provide robust evidence that competition indirectly influences tree growth by modifying their phenotypic traits. Notably, the crown, serving as a crucial organ for nutrient acquisition in trees, was a mediating factor between competition and growth. …”
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pDC Activation by TLR7/8 Ligand CL097 Compared to TLR7 Ligand IMQ or TLR9 Ligand CpG by Jing Wu, Shuang Li, Tete Li, Xinping Lv, Mingyou Zhang, Guoxia Zang, Chong Qi, Yong-Jun Liu, Liang Xu, Jingtao Chen

“…Moreover, we observed a distinctive morphological, phenotypic change in pDCs after activation by IMQ and CpG A. …”
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TB drug susceptibility testing in high fluoroquinolone resistance settings by F. Saluzzo, F. Masood, V. Batignani, F. Di Marco, U. Majeed, A. Ghazal, D.M. Cirillo, S. Tahseen

“…Xpert® MTB/RIF Ultra, MTB/XDR, Genotype® MTBDRplus, Genotype® MTBDRsl, culture, and phenotypic drug susceptibility testing (pDST) for relevant drugs (including bedaquiline and levofloxacin) were performed. …”
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Infectious bursal disease virus: identification of the novel genetic group and reassortant viruses by L. O. Scherbakova, Ye. V. Ovchinnikova, T. N. Zybina, S. N. Kolosov, N. G. Zinyakov, Z. B. Nikonova, D. B. Andreychuk, I. A. Chvala

“…However, not all isolates belonging to one genetic group have the same phenotypic characteristics. This is related to the fact that the virulence is determined not only based on the characteristics of the VP2 gene (A segment) but on the characteristics of the VP1 gene (B segment) as well. …”
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Closer look at circulating nitric oxide levels and their association with polycystic ovary syndrome: A meta-analytical exploration by Seyed Sobhan Bahreiny, Akram Ahangarpour, Elnaz Harooni, Mahdi Amraei, Mojtaba Aghaei, Reza Mohammadpour Fard

“…Subgroup analyses further demonstrated variations in NO levels between different PCOS phenotypes and in relation to metabolic parameters. …”
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The impact of nutritional status on centenarians’ physical, mental, and functional health by Ivan David Lozada-Martinez, Laura Vindas-Meza, Sandra Castelblanco-Toro, Juan Carlos Salazar-Uribe, Juan-Manuel Anaya

“…Summary: Background &amp; Aims: Nutrition has been described as a lifestyle factor strongly associated with health phenotypes in extreme longevity. However, little is known about its relationship with health-related outcomes in centenarians. …”
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Open-Label Fosmetpantotenate, a Phosphopantothenate Replacement Therapy in a Single Patient with Atypical PKAN by Yiolanda-Panayiota Christou, George A. Tanteles, Elena Kkolou, Annita Ormiston, Kostas Konstantopoulos, Maria Beconi, Randall D. Marshall, Horacio Plotkin, Kleopas A. Kleopa

“…Pantothenate kinase-associated neurodegeneration (PKAN) is an autosomal recessive disorder with variable onset, rate of progression, and phenotypic expression. Later-onset, more slowly progressive PKAN often presents with neuropsychiatric as well as motor manifestations that include speech difficulties, progressive dystonia, rigidity, and parkinsonism. …”
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