Interactions of the emerging fungus Candida auris with Acanthamoeba castellanii reveal phenotypic changes with direct implications on the response to stress and virulence by Michele Ramos Valente, Lucas Martins Alcântara, Deborah Santos Cintra, Susana Ruiz Mendoza, Elisa Gonçalves Medeiros, Kamilla Xavier Gomes, Leandro Honorato, Marcos de Abreu Almeida, Carmen Baur Vieira, Joshua Daniel Nosanchuk, Diana Bridon da Graça Sgarbi, Marcia Ribeiro Pinto, Leonardo Nimrichter, Allan Jefferson Guimarães

“…It may be essential to understand the various interactions C. auris could perform in the environment, directly impacting the outcome of human infections under the One Health approach.…”
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Clonal hematopoiesis of indeterminate potential is a risk factor of gastric cancer: A Prospective Cohort in UK Biobank study by Zhihui Xi, Huolun Feng, Kunling Chen, Xin Guo, Dandan Zhu, Jiabin Zheng, Yong Li

“…Exposures: Whole-exome sequencing was performed on blood samples collected at baseline. A CHIP status was identified based on the mutations on 43 CHIP-related genes. …”
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Evaluation of health care providers’ ability to identify patient-ventilator triggering asynchrony in intensive care unit: a translational observational study in China by Shengjun Liu, Zhangyi Zhao, Xiangyu Chen, Yi Chi, Siyi Yuan, Fuhong Cai, Zhangwei Song, Yue Ma, Huaiwu He, Longxiang Su, Yun Long

“…Professionals who published more than 2 articles in the field of critical respiratory performed better on auto-triggers [41.7% vs. 15.9% (p = 0.001)] and reverse triggers [38.9% vs. 19.2% (p = 0.018)]. …”
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PINK1 modulates Prdx2 to reduce lipotoxicity‐induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction by Hao Zhang, Tianyu Xu, Xiyuan Mei, Qiming Zhao, Qiling Yang, Xianghui Zeng, Zhuang Ma, Haobin Zhou, Qingchun Zeng, Dingli Xu, Hao Ren

“…Immunoprecipitation, western blotting and immunofluorescence analysis were performed to elucidate the molecular mechanisms involved. …”
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A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response by Emilie Crouchet, Nuno Almeida, Sarah C. Durand, Marie Parnot, Marine A. Oudot, Fabio Giannone, Cloé Gadenne, Natascha Roehlen, Antonio Saviano, Emanuele Felli, Patrick Pessaux, Hong Tuan Duong, Hideki Ohdan, Hiroshi Aikata, Kazuaki Chayama, Thomas F. Baumert, Catherine Schuster

“…Characterization of the tumor spheroid cell populations was performed by flow cytometry, immunohistochemistry (IHC), and functional assays. …”
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The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells by Weronika Zarychta-Wiśniewska, Anna Burdzińska, Katarzyna Zielniok, Marta Koblowska, Kamila Gala, Piotr Pędzisz, Roksana Iwanicka- Nowicka, Anna Fogtman, Aleksandra Aksamit, Agnieszka Kulesza, Aleksandra Zołocińska, Leszek Pączek

“…The microarray analysis was performed on RNA isolated from hASC and hBM-MSC A cells. …”
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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…The immunofluorescence staining, flow cytometry, qPCR, single-cell contraction, intracellular calcium transients, patch-clamp, dual luciferase reporter assay and western blot were performed for the study. Results Modeling TTC with high doses of epinephrine (Epi) treatment in hiPSC-CMs shows suppression of depolarization velocity (Vmax), prolongation of action potential duration (APD), and induction of arrhythmic events. …”
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Target trial emulation to evaluate the effect of immune-related adverse events on outcomes in metastatic urothelial cancer by Renate Pichler, Josef Fritz, Sarah Maier, Melanie R. Hassler, Johanna Krauter, David D`Andrea, Ekaterina Laukhtina, Kilian Gust, Keiichiro Mori, Karl H. Tully, Dora Niedersuess-Beke, Lea Korber, Jasmin Alija Spiegelberg, Thomas Bauernhofer, José D. Subiela, Roman Mayr, Andreas Kronbichler, Marco Moschini, Jeremy Teoh, Benjamin Pradere, Shahrokh F. Shariat, Hanno Ulmer, Laura S. Mertens, European Association of Urology–Young Academic Urologists (EAU-YAU): Urothelial Carcinoma Working Group

“…Methods TTE was contrasted to adjusted naïve and time-updated Cox models. We performed a multi-institutional retrospective study involving mUC patients under ICI. …”
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Epithelial NSD2 maintains FMO‐mediated taurine biosynthesis to prevent intestinal barrier disruption by Yue Xu, Xiuying Xiao, Chunxiao Ma, Ziyi Wang, Wenxin Feng, Hanyu Rao, Wei Zhang, Ningyuan Liu, Rebiguli Aji, Xiangjun Meng, Wei‐Qiang Gao, Li Li

“…Finally, rescue experiments were performed to confirm the molecular mechanism of NSD2 in the development of IBD. …”
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Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response by Aquib Ehtram, Mohd Shariq, Neha Quadir, Neha Quadir, Salma Jamal, Manjunath Pichipalli, Sheeba Zarin, Sheeba Zarin, Javaid Ahmad Sheikh, Nasreen Z. Ehtesham, Seyed E. Hasnain, Seyed E. Hasnain

“…Functional assays, including in vitro cytokine production and antigen presentation studies, were performed using TLR2/Myd88 knockout and wild-type macrophages. …”
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Therapeutic role of miR-26a on cardiorenal injury in a mice model of angiotensin-II induced chronic kidney disease through inhibition of LIMS1/ILK pathway by Weijie Ni, Yajie Zhao, Jinxin Shen, Qing Yin, Yao Wang, Zuolin Li, Taotao Tang, Yi Wen, Yilin Zhang, Wei Jiang, Liangyunzi Jiang, Jinxuan Wei, Weihua Gan, Aiqing Zhang, Xiaoyu Zhou, Bin Wang, Bi-Cheng Liu, Yuanyuan Ji

“…We generated an microRNA (miR)-26a knockout (KO) mouse model to investigate the role of miR-26a in angiotensin (Ang)-II-induced cardiac and renal injury. We performed Ang-II modeling in wild type (WT) mice and miR-26a KO mice, with six mice in each group. …”
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Impact of Immune Checkpoint Inhibitors and Local Radical Treatment on Survival Outcomes in Synchronous Oligometastatic NSCLC by Mandy Jongbloed, MD, Valentina Bartolomeo, MD, Martina Bortolot, MD, Shahan Darwesh, MD, Jarno W.J. Huijs, MD, Safiye Dursun, MD, Juliette Degens, MD, PhD, Ben E.E.M. van den Borne, MD, PhD, Maggy Youssef-El Soud, MD, PhD, Marcel Westenend, MD, PhD, Cordula Pitz, MD, PhD, Dirk K.M. De Ruysscher, MD, PhD, Lizza E.L. Hendriks, MD, PhD

“…Subgroup analyses were performed for patients who were deemed candidates for LRT in the multidisciplinary meeting and those proceeding to LRT. …”
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Global comparison of the economic costs of coronary heart disease: a systematic review and meta-analysis by Lan Gao, Susan Brumby, Crystal Man Ying Lee, George Mnatzaganian, Suzanne Robinson, Helen Brown, Rachel Huxley, Anna Peeters, Dan Xu, James Boyd, Sean Randall, Adrienne O’Neil, Sangita Shakya, Sarah Gauci, Anita Shrestha, James Lucas

“…Meta-regression and bias assessment were performed.Results Out of 20 100 records identified, 37 studies (including 2 564 189 individuals) from 22 countries were included in the qualitative synthesis and the quantitative meta-analysis. …”
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Residence-based disparities of composite index of anthropometric failures in East African under five children; multivariate decomposition analysis by Aznamariam Ayres, Yawkal Tsega, Abel Endawkie, Shimels Derso Kebede, Eyob Tilahun Abeje, Ermias Bekele Enyew, Chala Daba, Lakew Asmare, Amare Muche, Fekade Demeke Bayou, Mastewal Arefaynie, Asnakew Molla Mekonen, Abiyu Abadi Tareke, Awoke Keleb, Kaleab Mesfin Abera, Natnael Kebede, Endalkachew Mesfin Gebeyehu

“…The country specific survey data analysis was performed. Results The current study revealed that the burden of composite index of anthropometric failure (CIAF) in under-5 children were 40.69%, 22.04%, 34.06%, 31.99%, and 33.27% in Ethiopia, Kenya, Rwanda, Uganda, and Tanzania respectively. …”
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Identification of serotype O3b and high-risk clone ST37 of Klebsiella pneumoniae revealed by comparative genomic analysis by Sivaraman Gopalan Krishnan, Sudha Sajeev, Visnuvinayagam Sivam, Raja Swaminathan T., Rakshit Ojha, Bibek Ranjan Shome, Mark Holmes, Thanigaivel Sundaram, Ramesh M. D., Saranya Vinayagam, Suseela Meesala, Tabarak Malik, Tabarak Malik, Pavan Kumar Dara

“…The present study aims to investigate the prevalence of K. pneumoniae in fish available in the retail market using WGS.MethodsIsolation of K. pneumoniae, identification of K. pneumoniae isolates, and determination of the minimum inhibitory concentration (MIC) were performed. Whole-genome sequencing of K. pneumoniae genomes and phylogenomic analysis were conducted for visual comparison of the genomes. …”
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Research involvement among undergraduate medical students in Bangladesh: a multicenter cross-sectional study by Mohammad Jahid Hasan, Salwa Islam, Hasnat Sujon, Fahmida Islam Chowdhury, Muna Islam, Mostak Ahmed, Mahbuba Shabnam, Mahbub Mayukh Rishad, Mohammad Nurunnabi, Md Marufur Rahman, Jannatul Fardous, Mir Susmita Zaman, Mir Paramita Zaman, Mir Ahsan Ali, Md Rabiual Awal, Taha Choudhury, Ahmed Ullah Shuvo, Sabrina Sultana, Tanjina Tabassum, Monjur Rahman, Anindita Das Barshan, Tamanna Tabassum, Md. Abdur Rafi, Sharmeen Yasmeen

“…Statistical analyses were performed using SPSS 25. Results The mean age of the students was 21.8 ± 1.8 (SD) years, with the majority being female (61.8%). …”
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The Application of Magnetic-Controlled Capsule Gastroscopy in Patients Refusing C-EGD: A Single-Center 5-Year Observational Study by Lihan Zhou, Sijia Wang, Jian Li, Jie Zhong, Ling Zhang, Ruizhe Shen, Bielike Kouken, Chunhua Zhou, Qi Wang, Yuting Qian, Duowu Zou, Ye Chu

“…We aimed to explore the efficiency and safety of magnetic-controlled capsule gastroscopy (MCCG) in symptomatic outpatients who refused C-EGD. Methods. We performed a retrospective study of 76794 consecutive symptomatic outpatients from January 2014 to October 2019. …”
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Steatohepatitis-induced vascular niche alterations promote melanoma metastasis by Johannes Hoffmann, Julia Schüler, Bianca Dietsch, Sina Wietje Kürschner-Zacharias, Carsten Sticht, Felix A. Trogisch, Maren Schreitmüller, Tinja Baljkas, Kai Schledzewski, Manuel Reinhart, Sebastian A. Wohlfeil, Manuel Winkler, Christian David Schmid, Joerg Heineke, Cyrill Géraud, Sergij Goerdt, Philipp-Sebastian Reiners-Koch, Victor Olsavszky

“…Bulk RNA-Seq of isolated LSECs from CDAA-fed and control mice was performed. Levels of adhesion molecules (VCAM1, ICAM1, E-selectin) were monitored, and ICAM1 and VCAM1 antibody therapy was employed. …”
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Characteristics and multi-omics analysis of spontaneous spondyloarthritis in non-human primates: Case report by Lei Cai, Qing Lv, Ronghua Ma, Wei Liu, Yalun Guan, Zhongqiang Huang, Qingyu Liu, Yunfeng Li, Shuhua Liu, Ge Li, Yu Zhang

“…Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on these co-expressed genes and proteins, indicating a predominant enrichment in the IL-17 signaling pathway, with S100A8 and S100A9 identified as the core proteins. …”
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Characteristics of adherent and non-adherent patients with hypertension in a Norwegian cross-sectional study by Eirik Olsen, Lene V. Halvorsen, Stine Rognstad, Arleen Aune, Karl Marius Brobak, Ola Undrum Bergland, Vibeke N. Kjær, Knut Liestøl, Marit D. Solbu, Rune Mo, Eva Gerdts, Morten Rostrup, Sverre E. Kjeldsen, Aud Høieggen, Mimi S. Opdal, Anne Cecilie K. Larstorp, Camilla L. Søraas

“…Methods: Patients with hypertension using ≥ 2 antihypertensive agents were invited to participate in this national study performed in a semi-blinded fashion. Exclusion criteria were dementia, substance or alcohol abuse, pregnancy, terminal illnesses, poor Norwegian language skills, or severe kidney failure [glomerular filtration rate (GFR) < 30 mL/min/1.73 m2]. …”
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