Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line by Nafi’u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris

“…Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route. …”
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The Combination of Host-Associated Bacillus megaterium R32 and Stachyose Promotes the Intestinal Health of Turbot (Scophthalmus maximus. L) by Yaoyao Kong, Sifan Zhao, Weihao Ou, Kangsen Mai, Yanjiao Zhang

“…The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (β-catenin, CyclinD1, BAX, Bid); diets RS and RC significantly increased the expression of intestinal tight junction protein claudin-4, while RS group significantly decreased the expression of myosin light chain kinase; diets RS and RC significantly decreased the expression of proinflammatory factors (IL-13, IL-15, IFN- γ), diet RS also significantly decreased the expression of TNF-α and AP-1, and increased the expression of TGF-β. 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (Corynebacterium, Desulfovibrio, Escherichia-Shigella). …”
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Two-hit mouse model of heart failure with preserved ejection fraction combining diet-induced obesity and renin-mediated hypertension by Justin H. Berger, Yuji Shi, Timothy R. Matsuura, Kirill Batmanov, Xian Chen, Kelly Tam, Mackenzie Marshall, Richard Kue, Jiten Patel, Renee Taing, Russell Callaway, Joanna Griffin, Attila Kovacs, Dinesh Hirenallur-Shanthappa, Russell Miller, Bei B. Zhang, Rachel J. Roth Flach, Daniel P. Kelly

“…The HFD-Renin mouse model recapitulates key features of human HFpEF and will enable studies dissecting the contribution of individual pathogenic drivers to this complex syndrome. Additional preclinical HFpEF models allow for orthogonal studies to increase validity in assessment of interventions.…”
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The Imbalance of FOXP3/GATA3 in Regulatory T Cells from the Peripheral Blood of Asthmatic Patients by Tiantian Chen, Xiaoxia Hou, Yingmeng Ni, Wei Du, Huize Han, Youchao Yu, Guochao Shi

“…Treg cells play an important role in the pathogenic progress of asthma. Objective. To address the alterations of Treg cells in asthma. …”
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Helicobacter pylori promotes intestinal flora imbalance and hepatic metabolic disorders under arsenic stress by Linmin Zhong, Qiling Yang, Yiming Shao, Shanwen Hu, Lianxian Guo

“…Therefore, this study aims to investigate the combined effects and underlying pathogenic mechanisms of concurrent exposure to these two hazardous factors by establishing a mouse model that is infected with H. pylori and exposed to inorganic arsenic through drinking water. …”
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The landscape of rare mitochondrial DNA variants in sudden cardiac death: A potential role for ATP synthase by Elena Luppi, Monica De Luise, Carla Bini, Guido Pelletti, Gaia Tioli, Ivana Kurelac, Luisa Iommarini, Susi Pelotti, Giuseppe Gasparre

“…No known or novel mtDNA pathogenic variants were found. Two rRNA and 8 missense variants were rarer than polymorphisms as they had a frequency lower than 1% in population databases. 5/8 missense variants clustered in ATP synthase genes and 4/8 missense variants were previously detected in patients with suspected mitochondriopathy. …”
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Exploring the antifungal potential of Cannabis sativa-derived stilbenoids and cannabinoids against novel targets through in silico protein interaction profiling by Kevser Kübra Kırboğa, Aman Karim, Ecir Uğur Küçüksille, Mithun Rudrapal, Johra Khan, Raghu Ram Achar, Ekaterina Silina, Natalia Manturova, Victor Stupin

“…The proteins investigated included Glycosylphosphatidylinositol (GPI), Enolase, Mannitol-2-dehydrogenase, GMP synthase, Dihydroorotate dehydrogenase (DHODH), Heat shock protein 90 homolog (Hsp90), Chitin Synthase 2 (CaChs2), and Mannitol-1-phosphate 5-dehydrogenase (M1P5DH), all of which play crucial roles in fungal survival and pathogenicity. This research evaluates the binding affinities and interaction profiles of selected cannabinoids and stilbenoids with these eight proteins using molecular docking and molecular dynamics simulations. …”
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Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice by Ana Paula M. S. Brandão, Fernanda S. Oliveira, Natalia B. Carvalho, Leda Q. Vieira, Vasco Azevedo, Gilson C. Macedo, Sergio C. Oliveira

“…Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. …”
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Construction and efficacy of recombinant Newcastle disease virus co-expressing VP2 and VP3 proteins of very virulent infectious bursal disease virus by Tongfei Liu, Lin Lin, Yun Pan, Xiaoling Lin, Ming Liang, Guanming Shao, Keyu Feng, Yaxin Liu, Xinheng Zhang, Qingmei Xie

“…The genetic stability and growth pattern of rNDV were evaluated and its immunogenicity was assessed in specific pathogen free (SPF) chickens. rNDV-VP2-VP3 vaccines induced higher levels of neutralising antibodies, no damage to immune organs, and significantly lower viral loads in the bursa of the falciparum. rNDV-VP2 group showed partial protection, while the placebo group exhibited severe lesions and higher mortality, suggesting that the vaccine was effective in preventing IBDV-induced damage. …”
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A Novel Nonsense Mutation of POU4F3 Gene Causes Autosomal Dominant Hearing Loss by Chi Zhang, Mingming Wang, Yun Xiao, Fengguo Zhang, Yicui Zhou, Jianfeng Li, Qingyin Zheng, Xiaohui Bai, Haibo Wang

“…In this study, we describe a pathogenic nonsense mutation located in POU4F3 in a four-generation Chinese family. …”
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Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms by Chaitawat Sirisereewan, Thanh Che Nguyen, Nanthiya Iampraphat, Hongyao Lin, Leonardo Ellerma, Pisit Sirithanyakul, Roongtham Kedkovid, Roongroje Thanawongnuwech

“…Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. …”
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Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis by Nathalia Müller, Mateus Eugenio, Luciana F. Romão, Jorge Marcondes de Souza, Soniza V. Alves‐Leon, Loraine Campanati, Celso Sant’Anna

“…Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of microorganisms, including some pathogenic microorganisms. Herein, the antiproliferative effect of AgCl‐NPs on glioblastoma cell lines (GBM02 and GBM11) and on astrocytes was evaluated through automated quantitative image‐based analysis (HCA) of the cells. …”
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Thermosensitive In Situ Ophthalmic Gel for Effective Local Delivery and Antifungal Activity of Ketoconazole Nanoparticles by Chutima Chaiwut, Sarin Tadtong, Puriputt Akachaipaibul, Jutamas Jiaranaikulwanitch, Sudarshan Singh, Siriporn Okonogi, Dwi Marlina Syukri, Chuda Chittasupho

“…Fungal keratitis is a severe ocular infection caused by pathogenic fungi, leading to potential vision loss if untreated. …”
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A Sensitive, Specific and Fast Electrochemical‐based Nanobiosensor Diagnostic for Xanthomonas albilineans, the Cause of Sugarcane Leaf Scald Disease by Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Simon Strachan, Muhammad J.A. Shiddiky, Nam‐Trung Nguyen, Narshone Soda, Rebecca Ford

“…This EC‐based assay can be a commercially viable alternative, providing a DNA isolation/purification‐free solution, and can potentially be adapted into a handheld device for on‐farm detection and quantification of the LS‐causing pathogen.…”
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Reprogramming of iPSCs to NPCEC-like cells by biomimetic scaffolds for zonular fiber reconstruction by Tianhui Chen, Zhongxing Chen, Juan Du, Min Zhang, Zexu Chen, Qingyi Gao, Aodong Chen, Qinghao Meng, Yang Sun, Yan liu, Linghao Song, Xinyue Wang, Pranav Prakash Edavi, Chen Xu, Hongmei Zhang, Jinhai Huang, Yongxiang Jiang

“…Ocular tissue and aqueous humour samples from patients with EL were subjected to RNA sequencing and Olink high-throughput proteomic analysis, revealing the downregulation of pathogenic genes (FBN1, MFAP2) and upregulation of secretory proteins (IL-12, MMP-1). …”
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On‐chip label‐free impedance‐based detection of antibiotic permeation by Jaspreet Kaur, Hamed Ghorbanpoor, Yasin Öztürk, Özge Kaygusuz, Hüseyin Avcı, Cihan Darcan, Levent Trabzon, Fatma D. Güzel

“…In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. …”
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Trichoderma virens XZ11-1 producing siderophores inhibits the infection of Fusarium oxysporum and promotes plant growth in banana plants by Haiyang Cui, Qifeng Cheng, Tao Jing, Yufeng Chen, Xiaojuan Li, Miaoyi Zhang, Dengfeng Qi, Junting Feng, Farinaz Vafadar, Yongzan Wei, Kai Li, Yankun Zhao, Dengbo Zhou, Jianghui Xie

“…Use of antagonistic microbes to manage soil-borne pathogen is viewed as a promising strategy. Results Strain XZ11-1 isolated from tropical rainforest has the production ability of high siderophore. …”
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Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Cond... by Tatiane Aparecida Nascimento, Maria José Paes, Fernando Hercos Valicente, Margareth Maria de Carvalho Queiroz

“…The high pathogenic activity of <i>Bacillus thuringiensis</i> (<i>Bt</i>) strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. …”
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A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China by Zaixing Yang, Zaixing Yang, Yifan Li, Yuxuan Jiang, Yuxuan Jiang, Jingqi Wu, Zhenhong Guan, Junwei Ge, Lili Zhao

“…Aleutian mink disease (mink plasmacytosis) is a severe immune complex-mediated condition caused by the Aleutian Mink Disease Virus (AMDV), the most significant pathogen affecting mink health in the industry. Several studies have shown that AMDV epidemics can result in millions to tens of millions of dollars in economic losses worldwide each year. …”
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ANALYSIS OF CHANGES IN AFRICAN SWINE FEVER VIRUS GENETIC STRUCTURE AND BIOLOGICAL PROPERTIES DURING ADAPTATION TO CONTINUOUS CELL CULTURE by Ali Mazloum, N. G. Zinyakov, A. S. Pershin, I. V. Shevchenko, I. Yu. Zhukov, D. N. Fedoseyeva, D. V. Sharypova, A. S. Igolkin, N. N. Vlasova

“…Comparative analysis of the virus biological characteristics and detection of differences in its genome structure affecting certain phenotypic features is a main method used for the virus basic pathogenicity and immunogenicity examination. The most interesting and effective approach to addressing this problem is an analysis of changes in the gene structure during ASF virus adaptation to replication in continuous cell culture. …”
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