Effects of saffron-grape intercropping on saffron flower number and rhizosphere microbial community by Yuanyuan Tao, Guifen Zhou, Xingchang Zhang, Mengqing Feng, Liqin Li, Xiaodong Qian

“…Additionally, functional analysis revealed that intercropping was significantly higher (P < 0.01) than monoculture in dark hydrogen oxidation, denitrification, nitrate denitrification, nitrous oxide denitrification, nitrite denitrification, and manganese oxidation. Plant pathogens decreased from 6.13% in monoculture to 2.46% in intercropping. …”
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Pre-existing IgG antibodies to HCoVs NL63 and OC43 Spike increased during the pandemic and after COVID-19 vaccination by Zahra Hasan, Kiran Iqbal Masood, Marc Veldhoen, Shama Qaiser, Marta Alenquer, Mishgan Akhtar, Sadaf Balouch, Junaid Iqbal, Yaqub Wassan, Shahneel Hussain, Khalid Feroz, Sajid Muhammad, Atif Habib, Akbar Kanji, Erum Khan, Afsar Ali Mian, Rabia Hussain, Maria Joao Amorim, Zulfiqar A. Bhutta

“…Preexisting immunity may be associated with increased protection against non-related pathogens such as, SARS-CoV-2. There is little information regarding endemic human coronaviruses (HCOVs) from Pakistan, which experienced a relatively low COVID-19 morbidity and mortality. …”
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Association study of the JAK/STAT signaling pathway with susceptibility to COVID-19 in moroccan patient and in-silico analysis of rare variants by Meriem El Houdi, Hanaa Skhoun, Meriem El Fessikh, Reda Benmansour, Fatima-Zahra El Yousfi, Chaimae Nebhani, Mohamed Rida Tagajdid, Idriss Lahlou Amine, Hicham El Annaz, Rabii Ameziane El Hassani, Zohra Ouzzif, Redouane Abouqal, Khalid Ennibi, Ahmed Bouhouche, Jamila El Baghdadi

“…YASARA View allowed local 3D analysis comparing native and variant structures for pathogenic rare variants.The study encompassed 206 COVID-19 patients, averaging 45.70 ± 12.73 years and a control group (N=118). …”
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Importance of EQA/PT for the detection of genetic variants in comprehensive cancer genome testing by Kazuyuki Matsushita, Takayuki Ishige, Kousuke Watanabe, Toshiaki Akahane, Akihide Tanimoto, Michiko Yoshimoto, Munekazu Yamakuchi, Teruto Hashiguchi, Yoshinaga Okugawa, Makoto Ikejiri, Toshikazu Yamaguchi, Tadashi Yamasaki, Mayu Takeda, Masaaki Hibi, Naoki Akiyama, Kaho Shimizu, Naonori Hashimoto, Hiroko Sato, Yoshinori Tanaka, Fumie Amari, the EQA working group of Japan Association for Clinical Laboratory Science(JACLS)

“…Second, when DNA was extracted from FFPE samples, pathogenic variants and variants with companion diagnostic indications were detected in all 10 participating laboratories. …”
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Fibroblast growth factor receptor 3 mutation promotes HSPB6-mediated cuproptosis in hypochondroplasia by impairing chondrocyte autophagy by Jing Chen, Dan He, Chengrun Yuan, Na Li, Baohong Shi, Conway Niu, Jiangfei Yang, Liangkai Zheng, Lin Che, Ren Xu

“…Conclusions: Our study provides the first evidence for the pathogenicity of a gain-of-function mutation in FGFR3 (G382D) using mouse and cell models, and it underscores the potential of targeting the HSPB6-ERK-Drp1-FDX1 axis as a novel therapeutic approach for HCH. …”
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Identification of biomarkers for the diagnosis of type 2 diabetes mellitus with metabolic associated fatty liver disease by bioinformatics analysis and experimental validation by Guiling Wu, Guiling Wu, Sihui Wu, Sihui Wu, Tian Xiong, Tian Xiong, Tian Xiong, You Yao, You Yao, Yu Qiu, Yu Qiu, Yu Qiu, Liheng Meng, Cuihong Chen, Xi Yang, Xi Yang, Xi Yang, Xinghuan Liang, Yingfen Qin

“…PPI analysis revealed two important modules of T2DM-related pathogenic genes in MAFLD, which contained 49 nodes, suggesting their involvement in cell interaction, inflammation, and other processes. …”
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Systematic collection, annotation, and pattern analysis of viral vaccines in the VIOLIN vaccine knowledgebase by Anthony Huffman, Mehul Gautam, Arya Gandhi, Priscilla Du, Lauren Austin, Kallan Roan, Jie Zheng, Yongqun He, Yongqun He

“…The annotated information included basic vaccine names, pathogens and diseases, vaccine components, vaccine formulations, and their induced host responses. …”
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Emerging carbapenem-resistant Klebsiella pneumoniae in a tertiary care hospital in Lima, Peru by Fiorella Krapp, Diego Cuicapuza, Guillermo Salvatierra, Jean P. Buteau, Catherine Amaro, Lizeth Astocondor, Noemí Hinostroza, Jan Jacobs, Coralith García, Pablo Tsukayama

“…In conclusion, our study provides a comprehensive picture of the intra-hospital dynamics of these emerging pathogens and provides a framework for studying their genomic diversity in the understudied South American region.IMPORTANCEThis study provides novel insights into the transmission and genetic diversity of carbapenem-resistant Klebsiella pneumoniae, a bacteria responsible for severe infections, with limited treatment options. …”
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«Green-Ligand» in Metallodrugs Design—Cu(II) Complex with Phytic Acid: Synthetic Approach, EPR-Spectroscopy, and Antimycobacterial Activity by Kseniya A. Koshenskova, Natalia V. Makarenko, Fedor M. Dolgushin, Dmitriy S. Yambulatov, Olga B. Bekker, Matvey V. Fedin, Sergei A. Dementev, Olesya A. Krumkacheva, Igor L. Eremenko, Irina A. Lutsenko

“…The biological activity of <b>1</b> in vitro against the non-pathogenic (model for <i>Mycobacterium tuberculosis</i>) strain of <i>Mycolicibacterium smegmatis</i> is comparable to the first-line drug for tuberculosis treatment, rifampicin.…”
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Adjuvant-free, self−assembling ferritin nanoparticle vaccine coupled with influenza virus hemagglutinin protein carrying M1 and PADRE epitopes elicits cross-protective immune respo... by Yongqiang Zhao, Shuangshuang Guo, Jia Liu, Yating Wang, Bo Wang, Chun Peng, Enqi Du, Enqi Du, Enqi Du

“…Importantly, intranasal immunization with HMP-NPs, without any adjuvant, induced efficient mucosal IgA responses and conferred complete protection against the H3N2 virus, as well as partial protection against the H1N1 and H9N2 viruses and significantly reduced lung viral loads.DiscussionOverall, our results indicated that the self-assembled nanovaccines increased the potency and breadth of the immune response against various influenza viruses and are a promising delivery platform for developing vaccines with broader protection against emerging influenza viruses and other pathogens.…”
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Identifying subgroup of severe community-acquired pneumonia based on clinical metagenomics, a multicenter retrospective cohort study by Mingqiang Wang, Mingqiang Wang, Yue Jin, Yue Jin, Wenxiao Zhang, Wenxiao Zhang, Ling Ye, Ling Ye, Huanzhang Shao, Huanzhang Shao

“…We collected data on potentially pathogenic species reported by clinical metagenomics and clinical information for all patients. …”
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An exceptionally rare case of a diffuse midline glioma with concomitant H3.1 K27M and G34R mutations in the HIST1H3C (H3C3) gene by Zita Reisz, Rita Pereira, Smitha Nevis, Alan Mackay, Leena Bhaw, Yura Grabovska, Ross Laxton, Valeria Molinari, Anna Burford, Barnaby Clark, Cristina Bleil, Bassel Zebian, Erika Pace, Annette Weiser, Fernando Carceller, Lynley Marshall, Andrew King, Istvan Bodi, Safa Al-Sarraj, Chris Jones, Matthew Clarke

“…Further molecular studies (whole exome, whole genome sequencing) revealed concurrent H3.1 K27M and G34R mutations (clonal, in the same reads) of H3C3, FGF11 and PIK3CA somatic variants, and a pathogenic germline NBN variant. The RNAseq profile clustered with H3K27M-mutant tumours. …”
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Rat models of postintracerebral hemorrhage pneumonia induced by nasal inoculation with Klebsiella pneumoniae or intratracheal inoculation with LPS by Ruihua Wang, Changlian Gan, Rui Mao, Yang Chen, Ru Yan, Geng Li, Tianqin Xiong, Jianwen Guo

“…Spearman’s correlation analyses further revealed that the reduction or depletion of some beneficial bacteria, such as Allobaculum and Faecalitalea, and the blooming of some opportunistic pathogens, such as Turicibacter, Dietzia, Corynebacterium and Clostridium_sensu_stricto_1 in Kp-induced SAP rats were associated with the disordered sphingolipid and Trp metabolism. …”
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Exploring anticancer, antioxidant, and antimicrobial potential of Aspergillus flavus, a fungal endophyte isolated from Dillenia indica leaf callus by Ashish Gupta, Brajesh Chandra Pandey, Mohd Yaseen, Renu Kushwaha, Madhavenda Shukla, Pratima Chaudhary, Partha Pratim Manna, Aparna Singh, Ida Tiwari, Gopal Nath, Nishi Kumari

“…They foster a symbiotic association with their host plants, frequently conferring advantages such as augmented growth, enhanced resilience to stressors, and safeguarding against pathogens. Study design: Dillenia indica is a medicinal tree of Dilleniaceae. …”
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Prevalence of Antibiotic Resistance Genes in Differently Processed Smoothies and Fresh Produce from Austria by Sonia Galazka, Valerie Vigl, Melanie Kuffner, Irina Dielacher, Kathrin Spettel, Richard Kriz, Norbert Kreuzinger, Julia Vierheilig, Markus Woegerbauer

“…The bacterial microbiome, potential pathogens, bacterial ARG carriers and competent bacteria able to take up ARGs were identified by Illumina 16S rRNA gene sequencing. …”
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Targeting the G-quadruplex as a novel strategy for developing antibiotics against hypervirulent drug-resistant Staphylococcus aureus by Maria Sultan, Maria Razzaq, Joohyun Lee, Shreyasi Das, Shrute Kannappan, Vinod Kumar Subramani, Wanki Yoo, Truc Kim, Hye-Ra Lee, Akhilesh K. Chaurasia, Kyeong Kyu Kim

“…Abstract Background The rapid emergence of multiple drug-resistant (MDR) bacterial pathogens and the lack of a novel antibiotic pipeline pose a serious threat to global healthcare. …”
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MiR- 146b-5p inhibits Candida albicans-induced inflammatory response through targeting HMGB1 in mouse primary peritoneal macrophages by Jing Cheng, Xiaoxing Tian, Chuanxin Wu, Jiaojiao Wang, Huiling Liu, Sha Cheng, Hang Sun

“…Background: Candida albicans (C. albicans) is one of the most common pathogens associated with deep fungal infection, which represents a serious threat to human health. …”
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Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance by Qiwen Tian, Hailv Ye, Xuan Zhou, Junyi Wang, Lifang Zhang, Wenxuan Sun, Chenxin Duan, Minyu Fan, Wei Zhou, Chuyun Bi, Qiong Ye, Aloysius Wong

“…Studies conducted on probiotics from increasingly popular health supplements have raised the possibility of transmitting ARGs to commensals in the human gut, concomitantly establishing a reservoir of ARGs and risking acquisition by opportunistic pathogens. Building on our previous study that reported multiple antibiotic resistance in probiotics of health supplements, in this research, we have attempted to detect their ARGs that may account for resistant phenotypes. …”
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Identification of serotype O3b and high-risk clone ST37 of Klebsiella pneumoniae revealed by comparative genomic analysis by Sivaraman Gopalan Krishnan, Sudha Sajeev, Visnuvinayagam Sivam, Raja Swaminathan T., Rakshit Ojha, Bibek Ranjan Shome, Mark Holmes, Thanigaivel Sundaram, Ramesh M. D., Saranya Vinayagam, Suseela Meesala, Tabarak Malik, Tabarak Malik, Pavan Kumar Dara

“…Whole-genome sequencing (WGS) has become a powerful tool to complement traditional microbiological characterization of foodborne pathogens. Moreover, K. pneumoniae has several species complexes (KpSC) and is very difficult to differentiate using routine microbiological methods. …”
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Prevalence and characterization of IncQ1α-mediated multi-drug resistance in Proteus mirabilis Isolated from pigs in Kunming, Yunnan, China by Hongmei Liu, Hongmei Liu, Hongmei Liu, Na Xia, Fanan Suksawat, Bundit Tengjaroenkul, Yue Hu, Xiaofeng Zhou, Xiaojiang Li, Cuiqin Huang, Yinli Bao, Qiong Wu, Chunrong Zhang, Sunpetch Angkititrakul, Bin Xiang, Xin Wu

“…BackgroundProteus mirabilis is a conditionally pathogenic bacterium that is inherently resistant to polymyxin and tigecycline, largely due to antibiotic resistance genes (ARGs). …”
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