Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp. by Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Alok K. Paul, Sonia M. Rodrigues Oliveira, Maria L. Pereira, Tajudeen O. Jimoh, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Ajoy K. Verma, Veeranoot Nissapatorn

“…Background and Aim: Acanthamoeba spp. are pathogenic microorganisms linked to severe infections in humans and animals, requiring a deeper understanding of their encystation process for effective diagnostics and research. …”
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Impact of COVID-19 on testing, positive cases, patient characteristics, and hospital contacts for respiratory syncytial virus, influenza, and pneumococcus in Danish children by Andreas Jensen, Lone Graff Stensballe

“…The frequency of testing for respiratory pathogens increased over time, with a rising proportion of healthy individuals without underlying chronic disease being tested. …”
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Frequent mutation of K-RAS in addition to BRAF V600E in Ameloblastoma – An immunohistochemical study by Aishwariya Mohanty, Abikshyeet Panda, Pallavi Mishra, Lipsa Bhuyan, Harish Kumar

“…Background: Ameloblastoma is the most frequently encountered, enigmatic odontogenic tumor whose etiopathogenesis is still controversial. Understanding the pathogenic mechanism involved in Ameloblastoma and its proliferation aids in determining the best treatment option at an early stage. …”
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A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis by Reyhaneh Sadat Moosavi-Kohnehsari, Mahnaz Jafari-Sohi, Tohid Piri-Gharaghie, Shakiba Tolou-Shikhzadeh-Yazdi, Mona Aghassizadeh-Sherbaf, Romina Hosseinzadeh

“…The results provide a fresh method for creating new immunological candidate multi-epitome genes by using the food-grade, non-pathogenic Lactococcus lactis as a protein cell factory. …”
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Deep-Sea Ecosystems as an Unexpected Source of Antibiotic Resistance Genes by Wei Zhang, Yingdong Li, Yunmeng Chu, Hao Liu, Hongmei Jing, Qianfeng Xia

“…Our metagenomics study employed short-read-based (SRB) and assembled-contig-based (ACB) methods to identify ARGs in deep-sea waters and sediments and assess their potential pathogenicity. SRB prediction was found to be more effective for studying the abundance and diversity of these genes, while combining both methods better illustrated the relationship of ARGs with the hosts. …”
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Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens by Gopu Sriram, Vaishali Prakash Natu, Intekhab Islam, Xin Fu, Chaminda Jayampath Seneviratne, Kai Soo Tan, Tong Cao

“…Toll-like receptors (TLRs) play a role in recognizing microbial pathogens and eliciting an innate immune response. …”
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Systemic Proteomic Analysis Reveals Distinct Exosomal Protein Profiles in Rheumatoid Arthritis by Qiu Qin, Ronghua Song, Peng Du, Chaoqun Gao, Qiuming Yao, Jin-an Zhang

“…These proteins not only take important parts in the vicious circle in the pathogenic process of RA but also serve as novel biomarkers in RA diagnosis and prognosis.…”
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Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity by Thunchanok Yaikhan, Kamonnut Singkhamanan, Pawarisa Luenglusontigit, Arnon Chukamnerd, Natakorn Nokchan, Nutwadee Chintakovid, Sarunyou Chusri, Rattanaruji Pomwised, Monwadee Wonglapsuwan, Kittinun Leetanaporn, Surasak Sangkhathat, Komwit Surachat

“…Abstract In this study, we conducted a comprehensive investigation into the Enterobacter cloacae complex (ECC), a group of notorious pathogens responsible for various hospital-acquired infections. …”
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Determination of the melanin and anthocyanin content in barley grains by digital image analysis using machine learning methods by E. G. Komyshev, M. A. Genaev, I. D. Busov, M. V. Kozhekin, N. V. Artemenko, A.  Y. Glagoleva, V. S. Koval, D. A. Afonnikov

“…The pigment composition of plant seed coat affects important properties such as resistance to pathogens, pre-harvest sprouting, and mechanical hardness. …”
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First Detection and Genomic Characterization of Linezolid-Resistant <i>Enterococcus faecalis</i> Clinical Isolates in Bulgaria by Tanya V. Strateva, Preslava Hristova, Temenuga J. Stoeva, Hristina Hitkova, Slavil Peykov

“…Linezolid is an oxazolidinone antibiotic and is considered a last-resort treatment option for serious infections caused by problematic Gram-positive pathogens, including vancomycin-resistant enterococci. …”
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In silico evaluation of missense SNPs in cancer-associated Cystatin A protein and their potential to disrupt Cathepsin B interaction by Shafaat Hossain, Omar Hamza Bin Manjur, Mst Sharmin Sultana Shimu, Tamanna Sultana, Mustafizur Rahman Naim, Shahariar Siddique, Abdullah Al Mamun, Md Miftaur Rahman, Md Abu Saleh, Md Rakibul Hasan, Tania Rahman

“…Cystatin A (CSTA) functions as a cysteine protease inhibitor by forming tight complexes with the cathepsins. Pathogenic mutations in the CSTA gene can disrupt this interaction, potentially leading to physiological ailments. …”
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In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products by Guesh Mulaw, Tesfaye Sisay Tessema, Diriba Muleta, Anteneh Tesfaye

“…The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. …”
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DJK-5, an anti-biofilm peptide, increases Staphylococcus aureus sensitivity to colistin killing in co-biofilms with Pseudomonas aeruginosa by Samuel J. T. Wardell, Deborah B. Y. Yung, Anupriya Gupta, Mihnea Bostina, Joerg Overhage, Robert E. W. Hancock, Daniel Pletzer

“…Biofilms, which are bacterial communities encased in an extracellular polysaccharide matrix, contribute to approximately 80% of these infections. In particular, pathogens such as Pseudomonas aeruginosa and Staphylococcus aureus are frequently co-isolated from the sputum of patients with cystic fibrosis and are commonly found in chronic wound infections. …”
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Broad Diversity of Fungi in Hospital Water by Máira Gazzola Arroyo, Adriano Menis Ferreira, Oleci Pereira Frota, Natalia Seron Brizzotti-Mazuchi, Jacqueline Tanury Macruz Peresi, Marcelo Alessandro Rigotti, Carlos Eduardo Macedo, Alvaro Francisco Lopes de Sousa, Denise de Andrade, Margarete Teresa Gottardo de Almeida

“…Considering these microorganisms, fungi are known to occur ubiquitously in the environment, including water, and some are pathogenic and may cause health problems, especially in immunocompromised individuals. …”
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Knockdown resistance (kdr)-associated organochlorine resistance in mosquito-borne diseases (Culex pipiens): A systematic review and meta-analysis by Ebrahim Abbasi, Salman Daliri, Asghar Talbalaghi, Fatemeh Mehrpouya, Maryam Hasanzadeh arab, Atena Aslvaeli, Mohammad Djaefar Moemenbellah-Fard

“…Background: Culex pipiens is the vector of a large number of pathogens in humans. Use of insecticides to deal with this vector is the most important way to controlling it. …”
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Profiling the diversity of the village chicken faecal microbiota using 16S rRNA gene and metagenomic sequencing data to reveal patterns of gut microbiome signatures by Mxolisi Nene, Mxolisi Nene, Nokuthula Winfred Kunene, Rian Pierneef, Rian Pierneef, Rian Pierneef, Khanyisile Hadebe

“…Taxonomic profiles and functional gene annotations were derived, focusing on microbial diversity, antibiotic resistance genes (ARGs), and potential zoonotic pathogens.ResultsTaxonomic analysis showed that the predominant phyla in both provinces were Firmicutes, Bacteroidota, Proteobacteria, and Actinobacteria. …”
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Detection of inversion with breakpoints in ARSB causing MPS VI by whole-genome sequencing: lessons learned and best practices by Yufeng Huang, Wenyue Deng, Hui Huang, Xiankai Zhang, Xiaohong Chen, Jian Ye, Sukun Luo, Ting Yu, Hui Yao, Hao Du, Xuelian He

“…IntroductionMucopolysaccharidosis type VI (MPSVI), an autosomal recessive lysosomal storage disorder caused by pathogenic variants in ARSB gene. Usually, whole exome sequencing (WES) can identify these variants, and if WES failed to detect causative variants, whole-genome sequencing (WGS) may be considered to investigate deep intronic variations and structural alterations in patients.MethodsWhole-exome sequencing (WES) and whole genome sequencing (WGS) were performed in a Chinese family having a boy with suspected diagnosis of MPS with macrocephaly, coarse facial features, broad forehead, thick lips, frontal bossing, craniosynostosis, blue spots, frequent upper respiratory infections, inguinal hernia, and dysostosis multiplex. …”
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Unmasking MRSA antibiotic resistance: the impact of environment, pollution, and healthcare quality—a nationwide exploration in China: a comprehensive analysis spanning 2014 to 2021... by Yi-Chang Zhao, Ming-Xuan Xiao, Zhi-Hua Sun, Jia-Kai Li, Huai-yuan Liu, Bi-Kui Zhang, Hua-Lin Cai, Wei Cao, Feng Yu, Miao Yan

“…Abstract Background The rise of antibiotic resistance has become a critical global health concern, resulting in numerous fatalities annually. Among resistant pathogens, methicillin-resistant Staphylococcus aureus (MRSA) poses a particularly severe and expanding threat. …”
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Evaluating comparative effectiveness of psychosocial interventions for persons receiving opioid agonist therapy for opioid use disorder: protocol for a systematic review by Brian Hutton, Beverley Shea, Kimberly Corace, David Moher, Danielle B Rice, Becky Skidmore, Kednapa Thavorn, Sheena Taha, Dianna Wolfe, Fatemeh Yazdi, Beth A Sproule, Melanie Willows, Patricia Poulin, Gary Garber, Amy Porath, Leila Esmaeilisaraji

“…Secondary outcomes will include self-reported opioid use, abstinence from illicit drugs, adherence to psychosocial therapy and opioid agonist therapy, risk for sexually transmitted disease, risk for blood borne pathogens, changes in mental health symptoms (eg, depression), measures of craving and changes in patients’ quality of life and relevant adverse events. …”
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Genetic and neurochemical profiles underlying cortical morphometric vulnerability to Parkinson’s disease by Su Yan, Jun Lu, Bingfang Duan, Hongquan Zhu, Tian Tian, Yuanyuan Qin, Yuanhao Li, Wenzhen Zhu

“…Conclusions: Collectively, the present study offered interpretation of cortical morphological alterations and the cortical pathogenic theory in PD from genetic and neurochemical perspectives, which inspire further research on new pharmacotherapeutic approaches.…”
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