First Detection and Genomic Characterization of Linezolid-Resistant <i>Enterococcus faecalis</i> Clinical Isolates in Bulgaria by Tanya V. Strateva, Preslava Hristova, Temenuga J. Stoeva, Hristina Hitkova, Slavil Peykov

“…Linezolid is an oxazolidinone antibiotic and is considered a last-resort treatment option for serious infections caused by problematic Gram-positive pathogens, including vancomycin-resistant enterococci. …”
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In silico evaluation of missense SNPs in cancer-associated Cystatin A protein and their potential to disrupt Cathepsin B interaction by Shafaat Hossain, Omar Hamza Bin Manjur, Mst Sharmin Sultana Shimu, Tamanna Sultana, Mustafizur Rahman Naim, Shahariar Siddique, Abdullah Al Mamun, Md Miftaur Rahman, Md Abu Saleh, Md Rakibul Hasan, Tania Rahman

“…Cystatin A (CSTA) functions as a cysteine protease inhibitor by forming tight complexes with the cathepsins. Pathogenic mutations in the CSTA gene can disrupt this interaction, potentially leading to physiological ailments. …”
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Broad Diversity of Fungi in Hospital Water by Máira Gazzola Arroyo, Adriano Menis Ferreira, Oleci Pereira Frota, Natalia Seron Brizzotti-Mazuchi, Jacqueline Tanury Macruz Peresi, Marcelo Alessandro Rigotti, Carlos Eduardo Macedo, Alvaro Francisco Lopes de Sousa, Denise de Andrade, Margarete Teresa Gottardo de Almeida

“…Considering these microorganisms, fungi are known to occur ubiquitously in the environment, including water, and some are pathogenic and may cause health problems, especially in immunocompromised individuals. …”
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Profiling the diversity of the village chicken faecal microbiota using 16S rRNA gene and metagenomic sequencing data to reveal patterns of gut microbiome signatures by Mxolisi Nene, Mxolisi Nene, Nokuthula Winfred Kunene, Rian Pierneef, Rian Pierneef, Rian Pierneef, Khanyisile Hadebe

“…Taxonomic profiles and functional gene annotations were derived, focusing on microbial diversity, antibiotic resistance genes (ARGs), and potential zoonotic pathogens.ResultsTaxonomic analysis showed that the predominant phyla in both provinces were Firmicutes, Bacteroidota, Proteobacteria, and Actinobacteria. …”
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Unmasking MRSA antibiotic resistance: the impact of environment, pollution, and healthcare quality—a nationwide exploration in China: a comprehensive analysis spanning 2014 to 2021... by Yi-Chang Zhao, Ming-Xuan Xiao, Zhi-Hua Sun, Jia-Kai Li, Huai-yuan Liu, Bi-Kui Zhang, Hua-Lin Cai, Wei Cao, Feng Yu, Miao Yan

“…Abstract Background The rise of antibiotic resistance has become a critical global health concern, resulting in numerous fatalities annually. Among resistant pathogens, methicillin-resistant Staphylococcus aureus (MRSA) poses a particularly severe and expanding threat. …”
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Evaluating comparative effectiveness of psychosocial interventions for persons receiving opioid agonist therapy for opioid use disorder: protocol for a systematic review by Brian Hutton, Beverley Shea, Kimberly Corace, David Moher, Danielle B Rice, Becky Skidmore, Kednapa Thavorn, Sheena Taha, Dianna Wolfe, Fatemeh Yazdi, Beth A Sproule, Melanie Willows, Patricia Poulin, Gary Garber, Amy Porath, Leila Esmaeilisaraji

“…Secondary outcomes will include self-reported opioid use, abstinence from illicit drugs, adherence to psychosocial therapy and opioid agonist therapy, risk for sexually transmitted disease, risk for blood borne pathogens, changes in mental health symptoms (eg, depression), measures of craving and changes in patients’ quality of life and relevant adverse events. …”
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Genetic and neurochemical profiles underlying cortical morphometric vulnerability to Parkinson’s disease by Su Yan, Jun Lu, Bingfang Duan, Hongquan Zhu, Tian Tian, Yuanyuan Qin, Yuanhao Li, Wenzhen Zhu

“…Conclusions: Collectively, the present study offered interpretation of cortical morphological alterations and the cortical pathogenic theory in PD from genetic and neurochemical perspectives, which inspire further research on new pharmacotherapeutic approaches.…”
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Unravelling hybridization in Phytophthora using phylogenomics and genome size estimation by Kris Van Poucke, Annelies Haegeman, Thomas Goedefroit, Fran Focquet, Leen Leus, Marília Horta Jung, Corina Nave, Miguel Angel Redondo, Claude Husson, Kaloyan Kostov, Aneta Lyubenova, Petya Christova, Anne Chandelier, Slavcho Slavov, Arthur de Cock, Peter Bonants, Sabine Werres, Jonàs Oliva Palau, Benoit Marçais, Thomas Jung, Jan Stenlid, Tom Ruttink, Kurt Heungens

“…Abstract The genus Phytophthora comprises many economically and ecologically important plant pathogens. Hybrid species have previously been identified in at least six of the 12 phylogenetic clades. …”
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Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022–2023... by Anthony Loor-Giler, Silvana Santander-Parra, Sara Castillo-Reyes, Martin Campos, Renán Mena-Pérez, Santiago Prado-Chiriboga, Luis Nuñez

“…Furthermore, it was discovered that in certain samples, a genetic modification at position 297 was connected to the virus’s pathogenicity.…”
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The role of cyclooxygenase-2 (COX-2) and inflammatory markers in the progress of Alport syndrome in Egyptian children by Moushira Zaki, Hisham A. Orban, Mohamed A. Shahba, Rehab S. I. Moustafa, Ahmed Adel, Fatina I. Fadel, Abeer Selim, Hala T. El-Bassyouni, Eman R. Youness

“…This contributes to the pathogenic mechanisms and advancement of the disease in Alport syndrome. …”
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Immune system effectors as biomarkers of prognosis after acute burns in a case-control study by Alejandro Ferrari, Lorena Di Croce, Sabrina Saavedra, Marisel Hartwig, Omar Perrone, María Alejandra Guzmán

“…Following the initial injury, it has been demonstrated that the immune system plays a key role in early inflammation, tissue regeneration, and the response against pathogens. In this study, the performance of laboratory determinations as biomarkers of prognosis in acute burned patients was evaluated in a retrospective case-control protocol. …”
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Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis by Virginie Carrière, Muhammad Imran Arshad, Jacques Le Seyec, Benjamin Lefevre, Muhammad Farooq, Aurélien Jan, Christelle Manuel, Laurence Touami-Bernard, Catherine Lucas-Clerc, Valentine Genet, Hugues Gascan, Jean-Philippe Girard, Frédéric Chalmel, Lucie Lamontagne, Claire Piquet-Pellorce, Michel Samson

“…We aimed to decipher its function by infecting IL-33-deficient mice (IL-33 KO) and their wild-type (WT) littermates with pathogenic mouse hepatitis virus (L2-MHV3). The IL-33 KO mice were more sensitive to L2-MHV3 infection exhibiting higher levels of AST/ALT, higher tissue damage, significant weight loss, and earlier death. …”
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Discovery of the bacterial HslV protease activators as lead molecules with novel mode of action by Aurangzeb Sana, Aurongzeb Muhammad, Shamim Shahbaz, Rashid Yasmeen, Khan Khalid Mohammed, Aziz Tariq, Alharbi Metab, Alasmari Abdullah F.

“…The objective of this research was to identify certain promising drug candidates that could excessively stimulate the HslV protease, leading to uncontrolled protein breakdown in the pathogens. Four dihydropyrimidone derivatives have been identified as potential activators of HslV protease exhibiting high docking scores, favorable binding patterns, and significant in vitro activation capabilities. …”
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Isolation and Genomic Characterization of a Novel Porcine Reproductive and Respiratory Syndrome Virus 1 from Severely Diseased Piglets in China in 2024 by Shuai Yang, Meng Cui, Chen Li, Ming Qiu, Xiaoyang Zhu, Yanhan Lin, Yifan Meng, Yuejia Qiu, Wenhao Qi, Hong Lin, Wanglong Zheng, Jianzhong Zhu, Kewei Fan, Nanhua Chen

“…Overall, this study not only supported the idea that PRRSV-1 is rapidly evolving in Chinese swine herds, but also pulled the alarm that novel PRRSV-1 isolates with potentially increased pathogenicity might already exist in China, although they are still rarely detected among Chinese pigs.…”
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Crimean-Congo haemorrhagic fever virus screening among African individuals in Spain: Lessons to learn by Helena Miriam Lorenzo Juanes, Amparo López-Bernus, Belen Vicente, Montserrat Alonso-Sardón, Beatriz Rodríguez Alonso, Josue Pendones Ulerio, Pedro Fernandez Soto, Juan Luis Muñoz Bellido, Antonio Muro, Moncef Belhassen-García

“…Although the risk is considered very low in these collectives, clinicians should be alert to the possibility of new CCHF cases, given the high pathogenicity of CCHFV and the risk of transmission.…”
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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders by Nincy Debeuf, Sahine Lameire, Manon Vanheerswynghels, Julie Deckers, Caroline De Wolf, Wendy Toussaint, Rein Verbeke, Kevin Verstaen, Hamida Hammad, Stijn Vanhee, Bart N Lambrecht

“…To overcome this detection problem, adoptive transfer of a cohort of T cells purified from T cell receptor (TCR) transgenic donors has been extensively used but is not readily available for emerging pathogens. Constructing TCR transgenic mice from T cell hybridomas is a labor-intensive and sometimes erratic process, since the best clones are selected based on antigen-induced CD69 upregulation or IL-2 production in vitro, and TCR chains are polymerase chain reaction (PCR)-cloned into expression vectors. …”
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A Novel Wild-Type <i>Lacticaseibacillus paracasei</i> Strain Suitable for the Production of Functional Yoghurt and Ayran Products by Ioanna Prapa, Chrysoula Pavlatou, Vasiliki Kompoura, Anastasios Nikolaou, Electra Stylianopoulou, George Skavdis, Maria E. Grigoriou, Yiannis Kourkoutas

“…In addition, strong adhesion capacity to differentiated Caco-2 cells (1.77–21.04%) was mapped, and the lactobacilli strains exhibited strong antagonistic activity against foodborne pathogens compared to lactococci. The strains were able to grow at low pH and high NaCl concentrations, conditions that prevail in food systems (cell counts ranged from 1.77 to 8.48 log CFU/mL after exposure to pH 3 and from 5.66 to 9.52 log CFU/mL after exposure to NaCl concentrations up to 8%). …”
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Diagnosis, screening, and follow-up of patients with familial interstitial lung disease: Results from an international survey by Emil Vilstrup Moen, Thomas Skovhus Prior, Michael Kreuter, Wim A. Wuyts, Maria Molina-Molina, Marlies Wijsenbeek, Antonió Morais, Argyrios Tzouvelekis, Christopher J. Ryerson, Fabian Caro, Ivette Buendia-Roldan, Jesper M. Magnusson, Joyce S. Lee, Julie Morisett, Justin M. Oldham, Lauren K. Troy, Manuela Funke-Chambour, Maria Laura Alberti, Raphael Borie, Simon L. F. Walsh, Sujeet Rajan, Yasuhiro Kondoh, Yet H Khor, Elisabeth Bendstrup

“…Many respondents were unaware of specific genetic testing methods. Pathogenic genetic variants were seen as a potential contraindication for lung transplantation in 6–8% of respondents. …”
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A novel TGFBR2 mutation causes Loeys-Dietz syndrome in a Chinese infant: A case report by Xin Liu, Kaiqing Liu, Lifu Hu, Zixiao Liu, Xinhua Liu, Jiantao Wang

“…The diagnosis of this disease is mainly based on clinical features combined with the detection of pathogenic gene mutations, mainly mutations in the transforming growth factor-beta (TGF-β) signaling pathway. …”
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Role of acute respiratory diseases in pathogenesis of distal limb infections in cattle by A. D. Alekseev, O. G. Petrova, M. I. Barashkin, I. M. Milshtein, V. D. Moskvin

“….), streptococcosis (Streptococcus spp.) and other pathogens. In addition, favorable conditions evolve for the development of mixed infection due to reduction in the overall organism resistance, which is observed for both respiratory and distal limb infections.…”
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