Leucine-rich alpha-2 glycoprotein in combination with C-reactive protein for predicting endoscopic activity in Crohn’s disease: a single-centre, cross-sectional study by Yoshiaki Takada, Hiroki Kiyohara, Yohei Mikami, Masataka Taguri, Ryoya Sakakibara, Yasuhiro Aoki, Kosaku Nanki, Takaaki Kawaguchi, Yusuke Yoshimatsu, Shinya Sugimoto, Tomohisa Sujino, Kaoru Takabayashi, Naoki Hosoe, Haruhiko Ogata, Motohiko Kato, Yasushi Iwao, Nobuhiro Nakamoto, Takanori Kanai

“…In diagnosing endoscopically moderate to severe activity (SES-CD > 6), the area under the ROC curve of LRG was greater than that of CRP (0.74 vs. 0.63; p = .037). The optimal cut-off value estimated by Youden’s index was 15.5 µg/mL for LRG, and 0.13 mg/dL for CRP. …”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The gene encoding natural proteinase K (PK-w) was designed and optimized for expression in K. phaffii K51. The proteinase K gene was synthesized and cloned within the plasmid pPICZα-A vector in E. coli TOP10 cells. …”
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Effects of tannic acid on growth performance, intestinal health, and tolerance in broiler chickens by Huiping Xu, Lu Gong, Xiaodan Zhang, Zhenyi Li, Jianyang Fu, Zengpeng Lv, Yuming Guo

“…This study investigated the optimal tannic acid dosage and assessed tolerance levels in broiler chickens. …”
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Ultrasound-assisted modification of oat protein isolates: Structural and functional enhancements by Hamad Rafique, Pai Peng, Xinzhong Hu, Kanza Saeed, Muhammad Zubair Khalid, Waseem Khalid, Sonia Morya, Tawfiq Alsulami, Robert Mugabi, Gulzar Ahmad Nayik

“…Moderate ultrasonic treatment (400 W) promoted protein dissolution, and maintained a good balance between β-sheets (71.04 ± 0.08), α-helix (16.27 ± 0.02) and β-turns (12.68 ± 0.03), exhibiting optimized flexibility and structural integrity. Whereas, higher strength (600 W) significantly destroyed protein structure. …”
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Technical progress of construction and safe operation of underground gas storage under complex geological conditions by Qiqi WANYAN, Yun WANG, Dongxu LI, Hongcheng XU, Chun LI, Kang LI, Jingcui LI, Lifeng LI

“…Further, efforts should be made to form an optimal design method of intensive injection and production, and to develop a domestic microseismic monitoring system. …”
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Association of daily step counts and step intensity with mortality among US adults: a cross–sectional study of NHANES 2005–2006 by Tianzhou Peng, Changhao Liu, Ti Yang, Leyi Liao, Qingping Li, Hanbiao Liang, Jiapeng Zhang, Chen Xie, Kai Wang, Chuanjiang Li

“…Our findings suggest that achieving 10,000 steps per day may be optimal for reducing the risk of all–cause mortality risk in this population.…”
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Development an Inflammation-Related Factor-Based Model for Predicting Organ Failure in Acute Pancreatitis: A Retrospective Cohort Study by Yunpeng Peng, Xiaole Zhu, Chaoqun Hou, Chenyuan Shi, Dongya Huang, Zipeng Lu, Yi Miao, Qiang Li

“…The AUC of the new score system was 0.86. The optimal cut-off value of the new scoring system was 6 points. …”
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Microbial Colonization and Antibiotic Resistance Profiles in Chronic Wounds: A Comparative Study of Hidradenitis Suppurativa and Venous Ulcers by Florica Sandru, Elena Poenaru, Smaranda Stoleru, Andreea-Maria Radu, Alexandra-Maria Roman, Corina Ionescu, Aurelian Zugravu, Jafal Mugurel Nader, Livia-Cristiana Băicoianu-Nițescu

“…Understanding microbial diversity and antibiotic resistance patterns is essential in order to optimize therapeutic strategies. This study aimed to investigate the microbial populations and antibiotic resistance profiles in HS and VU patients, comparing the prevalence of common pathogens and their antimicrobial resistance profiles. …”
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Addressing the gap in preterm resuscitation practices in high-income and low-middle income countries: a multicenter survey of the Asian neonatal network collaboration by Rinawati Rohsiswatmo, Rizalya Dewi, Jennie Sutantio, Zubair Amin, Young-Ah Youn, Sae Yun Kim, Su Jin Cho, Yun Sil Chang, Satoshi Kusuda, Fuyu Miyake, Tetsuya Isayama

“…Further enhancements are imperative to narrow this gap and optimize neonatal outcomes.…”
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Concentrations and exposure risks of per- and polyfluoroalkyl substances in human milk from Southern China during the first 400 days of lactation by Meng-Tao Yang, Fei Yang, Qiu-Ye Lan, Si-Yu Huang, Xiao-Yan Chen, Ying-Zhen Qiu, Hui-Lian Zhu

“…However, breastfeeding remains the optimal choice due to its significant health benefits. …”
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The Beneficial Application of Turmeric (Curcuma longa L.) on Health and Egg Production, in Layers: A Review by Saade Abdalkareem Jasim, Aiman Mohammed Baqir Al‐Dhalimy, Maryam Zokaei, Shadi Salimi, Mohammed Jawad Alnajar, Abhinav Kumar, Enas R. Alwaily, Ahmed Hussein Zwamel, Soura Alaa Hussein, Majid Gholami‐Ahangaran

“…However, further research is needed to fully understand the mechanisms of action, optimize dosage regimens and evaluate the long‐term effects of turmeric supplementation in layer diets.…”
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From bench to bedside: elucidating VEGF(R) inhibitor-related heart failure in cancer treatment by Shengkun Peng, MinHong Cai, Hongyu Kuang, Anqi Lin, Qinghua Ma, Xiaoqin Dai, Peng Luo, Yijun Liu, Guo Zhang, Yifeng Bai

“…Conclusions Our findings present the first comprehensive characterization of VirHF clinical features and elucidate its underlying molecular mechanisms, thereby providing a theoretical framework for optimizing the clinical safety of VEGF(R)i therapy.…”
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Hot press sintering of Y2O3-Al2O3 glasses: Impact of particle size on thermal behaviour, sintering ability and mechanical properties of sintered bodies by Melinda Majerová, Anna Prnová, Jana Valúchová, Monika Michálková, Beáta Pecušová, Michal Žitňan, Róbert Klement, Dušan Galusek

“…The work was conducted with the aim of determining the optimal particle size and grinding conditions of glassy powder used for hot-pressing of ceramic and glass-ceramic materials with desired mechanical properties. …”
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Multiple Gene Deletion Mutants of Equine Herpesvirus 1 Exhibit Strong Protective Efficacy Against Wild Virus Challenge in a Murine Model by Stephanie S. Pradhan, Vekataramireddy Balena, Bidhan Chandra Bera, Taruna Anand, Rhushikesh Khetmalis, Aashwina Madhwal, Supriya Kandasamy, Selvaraj Pavulraj, Manju Bernela, Priya Mor, Bhupendra Nath Tripathi, Nitin Virmani

“…Both mutants induced an optimal level of EHV1-specific humoral and cell-mediated immune responses, as determined by virus neutralization assay, ELISA, and immunophenotyping. …”
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Effect of carbon quantum dots on the anticorrosive action of the tin coatings by Taiane Fíngolo Duarte, Victor Magno Paiva, Braulio Soares Archanjo, Clara Muniz da Silva de Almeida, Natasha Midori Suguihiro, Rodrigo José Corrêa, Emerson Schwingel Ribeiro, Eliane D'Elia

“…The electrochemical tests show an optimal working concentration equivalent to 0.05 g L−1 of CDs in the bath. …”
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Assessing the detection and interaction of Lawsonia intracellularis and porcine circovirus 2 in low and high-performance wean-to-finish pig groups in different porcine reproductive... by Guilherme Cezar, Fernando L. Leite, Eduardo Fano, Reid Phillips, John Waddell, Kate Dion, Edison Magalhães, Giovani Trevisan, Gustavo Silva, Daniel C. Linhares

“…IntroductionEffective disease management strategies are essential for achieving optimal pig performance, ensuring high-quality animal health and welfare, and maintaining the economic viability of swine systems. …”
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Endothelial Activation and Stress Index (EASIX) to predict mortality after allogeneic stem cell transplantation: a prospective study by Axel Benner, Peter Dreger, Grzegorz W Basak, Christian Koenecke, Olaf Penack, Christophe Peczynski, Lucia López Corral, Ivan Moiseev, Hélène Schoemans, Zinaida Peric, Thomas Luft, Simona Sica, Mutlu Arat, Maija Itäla-Remes, Nicolaas P M Schaap, Michal Karas, Ludek Raida, Thomas Schroeder, Elisabetta Metafuni, Tulay Ozcelik, Brenda M Sandmaier, Lambros Kordelas

“…For broad clinical implementation, a prospectively validated EASIX-pre cut-off is needed that defines a high-risk cohort and is easy to use.Method In the current study, we first performed a retrospective cohort analysis in n=2022 alloSCT recipients and identified an optimal cut-off for predicting non-relapse mortality (NRM) as EASIX-pre=3. …”
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A network comparison on efficacy and safety profiling of PD-1/PD-L1 inhibitors in first-line treatment of advanced non-small cell lung cancer by Jie Fu, Jie Fu, Yi-Dan Yan, Xu Wan, Xiao-Fan Sun, Xiao-Fan Sun, Xiu-Mei Ma, Ying-Jie Su

“…Regarding OS, for patients with PD-L1 expression ≥50%, 1%–49% and <1%, camrelizumab + chemotherapy (HR 0.48, 95% CI: 0.21–1.11), cemiplimab + chemotherapy (HR 0.50, 95% CI: 0.32–0.79) and nivolumab + ipilimumab (HR 0.64, 95% CI: 0.51–0.81) were considered optimal treatments. Compared with chemotherapy, monotherapy with nivolumab, cemiplimab, pembrolizumab, atezolizumab and durvalumab had lower odds of TRAE grade ≥3.ConclusionIn all patients, penpulimab plus chemotherapy was the most effective therapy, but treatment preferences varied by PD-L1 expression, histology type and associated outcomes. …”
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Rapid Determination of Pemetrexed Concentration and Distribution in Human Breast Cancer Cells (McF-7) Based on UHPLC-MS/MS by Mengwei Zhang, Zhipeng Wang, Qiqiang Zhang, Qing Ye, Fengjing Xu, Lili Cui, Yanping Liu, Shouhong Gao, Wansheng Chen, Yan Liu

“…Sample pretreatment was completed by protein precipitation using methanol. The optimized chromatographic separation was achieved on a ZORBAX SB-C18 column (2.1 × 150 mm, 3.5 μm). …”
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Predicted indirectly recognizable HLA epitopes scores and clinical outcomes after haploidentical stem cell transplantation in pediatric patients with relapsed neuroblastoma by Eun Seop Seo, Eun Seop Seo, In Hwa Jeong, In Hwa Jeong, Hee Young Ju, Ju Kyung Hyun, Ji Won Lee, Keon Hee Yoo, Won Young Heo, Ki Woong Sung, Hee Won Cho, Eun-Suk Kang

“…Incorporating PIRCHE scores into donor selection is expected to optimize transplant outcomes.…”
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