Gut microbiota modulation in cardiac cell therapy with immunosuppression in a nonhuman primate ischemia/reperfusion model by Hung-Chih Chen, Yu-Che Cheng, Marvin L. Hsieh, Po-Ju Lin, Emily F. Wissel, Theodore Steward, Cindy M. C. Chang, Jennifer Coonen, Timothy A. Hacker, Timothy J. Kamp, Patrick C. H. Hsieh

“…Immunosuppression promoted anaerobes, such as Faecalibacterium, Streptococcus, Anaerovibrio and Dialister, and altered amino acid metabolism and nucleosides/nucleotides biosynthesis in host plasma. EC + CM cotreatment favors Phascolarctobacterium, Fusicatenibacter, Erysipelotrichaceae UCG-006, Veillonella and Mailhella. …”
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Brain-Derived Neurotrophic Factor Polymorphism and Aphasia after Stroke by Nathan T. Lee, Fatimah Ahmedy, Natiara Mohamad Hashim, Khin Nyein Yin, Kai Ling Chin

“…The remaining two studies have shown that Met allele genotype (either single or double nucleotides) was associated with poor aphasia recovery, in either acute or chronic stroke. …”
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Study on Effect of Producing Region on the Metabolome Composition of Rice by LIU Fu-ying, CHEN Xue-feng, WANG Xin, CHEN Guo-yan, GUO Ying, SUN Jing-han, YANG Shui-yan, NIE Xu-heng

“…The top 10 most significantly upregulated and downregulated metabolites with the largest fold changes in the 3 comparison groups were mainly flavonoids, phenolic acids, nucleotides and their derivatives. This study provides valuable insights into the metabolic composition and differences of rice from different producing regions. …”
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Plant MicroRNA Prediction by Supervised Machine Learning Using C5.0 Decision Trees by Philip H. Williams, Rod Eyles, Georg Weiller

“…MicroRNAs (miRNAs) are nonprotein coding RNAs between 20 and 22 nucleotides long that attenuate protein production. …”
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Use of miRNAs as Biomarkers in Sepsis by Raluca Dumache, Alexandru Florin Rogobete, Ovidiu Horea Bedreag, Mirela Sarandan, Alina Carmen Cradigati, Marius Papurica, Corina Maria Dumbuleu, Radu Nartita, Dorel Sandesc

“…Recent studies highlight the use of new biomarkers for sepsis, namely, miRNAs. miRNAs belong to a class of small, noncoding RNAs with an approximate content of 19–23 nucleotides. Following biochemical and physiological imbalances, the expression of miRNAs in blood or other body fluids changes significantly. …”
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Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions by Izabela Zaras, Olga Kujawa, Marcin Olszewski, Marta Jarczewska

“…Herein, we present studies on the electrochemical detection of lead ions using PS2M aptamer, which contains several guanine nucleotides, as the receptor element. Firstly, the method of aptamer-based layer fabrication was optimized along with the choice of a redox active indicator, which was a source of current signal. …”
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AFM observation of protein translocation mediated by one unit of SecYEG-SecA complex by Yui Kanaoka, Takaharu Mori, Wataru Nagaike, Seira Itaya, Yuto Nonaka, Hidetaka Kohga, Takamitsu Haruyama, Yasunori Sugano, Ryoji Miyazaki, Muneyoshi Ichikawa, Takayuki Uchihashi, Tomoya Tsukazaki

“…Additionally, the uniform unidirectional distribution of nanodiscs on a mica substrate enables the HS-AFM image data analysis, revealing dynamic structural changes in the polypeptide-crosslinking domain of SecA between wide-open and closed states depending on nucleotides. The nanodisc-AFM approach will allow us to execute detailed analyses of Sec proteins as well as visualize nano-scale events of other membrane proteins.…”
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The activation of Chlamydomonas reinhardtii alpha amylase 2 by glutamine requires its N‐terminal aspartate kinase–chorismate mutase–tyrA (ACT) domain by Lisa Scholtysek, Ansgar Poetsch, Eckhard Hofmann, Anja Hemschemeier

“…While none of the recombinant enzymes was directly affected by the presence of cGMP or other nucleotides, suggesting an indirect binding to cGMP, AMA2 activity was stimulated in the presence of L‐glutamine (Gln). …”
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The role of microRNAs in learning and long-term memory by L. N. Grinkevich

“…MicroRNAs are unique endogenous non-coding RNAs about 22 nucleotides in length; each can regulate translation of hundreds of messenger RNA targets, thereby controlling entire gene networks. …”
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Expression profiles of long and short RNA s in the cytoplasm and nuclei of growing chicken (Gallus gallus domesticus) oocytes by A. V. Krasikova, A. V. Fedorov

“…Three groups of short RNAs differing in size (from 20 to 40 nucleotides) were recognized in chicken oocytes. They might correspond to short regulatory RNA classes. …”
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Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host. by Martine Bangratz, Aurore Comte, Estelle Billard, Abdoul Kader Guigma, Guillaume Gandolfi, Abalo Itolou Kassankogno, Drissa Sérémé, Nils Poulicard, Charlotte Tollenaere

“…This method was validated by the successful reconstruction of two viral genomes that were less than a hundred nucleotides apart (out of a genome of 4450nt length, i.e. 2-3%), and present in artificial mixes at a ratio of up to a 99/1. …”
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Report of a Novel Mutation in MLH1 Gene in a Hispanic Family from Puerto Rico Fulfilling Classic Amsterdam Criteria for Lynch Syndrome by Juan M. Marqués-Lespier, Yaritza Diaz-Algorri, Maria Gonzalez-Pons, Marcia Cruz-Correa

“…A novel mutation at c.2044_2045 in hMLH1 consisting of the deletion of two consecutive nucleotides (AT) at exon 18 was identified. This deletion causes a frameshift in the protein coding sequence at p.682 resulting in premature termination and a truncated MLH1 protein. …”
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A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9 by Lan Hu, Jing Han, Hao-Da Wang, Zhou-Hua Cheng, Chang-Ce Lv, Dong-Feng Liu, Han-Qing Yu

“…The deaminase domain-inlaid FrCas9n exhibits an editing range covering 38 nucleotides upstream and downstream of the palindromic PAM, without context preference, which is 6.3 times larger than that of traditional CBEs. …”
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Profiling of small RNAs derived from tomato brown rugose fruit virus in infected Solanum lycopersicum plants by deep sequencing by Mangle Chen, Donghai Wang, Jiali Yang, Yuhao Cao, Xuemei Song, Yuwen Lu, Hongying Zheng, Jiejun Peng, Guanwei Wu, Jian Wu, Junmin Li, Jianping Chen, Fei Yan, Shaofei Rao

“…VsiRNAs were mainly 21 and 22 nucleotides in length and had a U-bias at the 5′ end. …”
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Shedding light on DNA methylation and its clinical implications: the impact of long-read-based nanopore technology by Alexandra Chera, Mircea Stancu-Cretu, Nicolae Radu Zabet, Octavian Bucur

“…Interestingly, nanopore sequencing can also extract epigenetic status of the nucleotides (including both 5-Methylcytosine and 5-hydroxyMethylcytosine), and a large variety of bioinformatic tools have been developed for improving its detection properties. …”
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High-throughput single-molecule quantification of individual base stacking energies in nucleic acids by Jibin Abraham Punnoose, Kevin J. Thomas, Arun Richard Chandrasekaran, Javier Vilcapoma, Andrew Hayden, Kacey Kilpatrick, Sweta Vangaveti, Alan Chen, Thomas Banco, Ken Halvorsen

“…Hybrid stacking with phosphorylated, methylated, and RNA nucleotides had no measurable effect, but a fluorophore modification reduced stacking energy. …”
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Epidermal Growth Factor Receptor Exon 19 Deletion Subtypes Do Not Influence Survival Outcomes Following First-line Epidermal Growth Factor Receptor-tyrosine Kinase Inhibitors in Ad... by Yan-Jei Tang, John Wen-Cheng Chang, Chen-Yang Huang, Yueh-Fu Fang, Ching-Fu Chang, Cheng-Ta Yang, Chih-Hsi Scott Kuo, Ping-Chih Hsu, Chiao-En Wu

“…E746_A750 mutation, a common 15 nucleotide deletion, or a pure deletion without insertion/substitution. …”
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Taxonomic assessment of the Oxytropis species from South-East of Kazakhstan by S. Almerekova, S. Abugalieva, N. Mukhitdinov

“…Out of the 601 aligned positions of ITS 33 (5.6 %) sites were found to be polymorphic nucleotides and used in evaluation of the genetic relationship of species. …”
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Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells by Fumiko Taniuchi, Koji Higai, Tomomi Tanaka, Yutaro Azuma, Kojiro Matsumoto

“…In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). …”
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Antisense oligonucleotides for the arterial hypertension mechanisms study and therapy by L. O. Klimov, A. A. Seryapina, V. F. Zarytova, A. S. Levina, A. L. Markel

“…The main modifications of antisense nucleotides, designed to increase the duration of their effects and simplify the delivery of this type of drugs to the targets are discussed, in particular, combining antisense oligonucleotides with adenovirus-based expression vectors. …”
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