Molecular Programming Design of Glyconucleic Acid Aptamer with High Stability by Yongqi Han, Rongjun Zhang, Hong‐Liang Bao, Mei Yang, Yuan Gao, Xiaobo Gao, Ruowen Wang, Weihong Tan, Ding‐Kun Ji

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Diagnostic accuracy of cartridge-based nucleic acid amplification test and resistance pattern in new and previously treated tuberculosis cases: A prospective observational study by Ranjit Suva, Gaurang Khadiya, Kinjal D. Rami, Urvashi Modh, Firoz Ghanchi, Yogesh Murugan

Subjects: “…cartridge-based nucleic acid amplification test…”
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Tumor derived cell‐free nucleic acid upregulates programmed death‐ligand 1 expression in neutrophil via intracellular Toll‐like receptor signaling by Suguru Saito, Duo‐Yao Cao, Tomohiro Shibata, Yan Liu, Aoi Otagiri‐Hoshi, Xiaojiang Cui, Kenneth E. Bernstein

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A preliminary evaluation of a fast, low-cost, and high-throughput nucleic acid extraction method for bacterial microbiota profiling in low-microbial biomass samples by Xiangning Bai, Simon Rayner, Linda Ellevog Skuggen, Kine Kragl Engseth, Pål Marius Bjørnstad, Marianne Dalland, Gregor D. Gilfillan, Magnar Bjørås, Andreas Matussek

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Evaluation of the performance of nucleic acid amplification tests (NAATs) in detection of chlamydia and gonorrhoea infection in vaginal specimens relative to patient infection status: a systematic review by Minttu M Rönn, Louise Mc Grath-Lone, Bethan Davies, Janet D Wilson, Helen Ward

“…Objective We evaluated the performance of nucleic acid amplification tests (NAATs) using vaginal specimens in comparison to specimens from the cervix or urine in their ability to detect chlamydia and gonorrhoea infection in women based on patient infection status (PIS).Design Systematic review.Data sources EMBASE and Ovid MEDLINE databases were searched through 3 October 2017.Eligibility criteria for selecting studies We included studies that tested samples from the vagina and ≥1 other site (cervix and/or urine) with ≥2 NAATs for chlamydia and ≥2 NAATs or 1 NAAT and culture for gonorrhoea for each site.Data extraction and synthesis Performance is defined as the sensitivity of a NAAT using a specimen site and PIS of the patient. …”
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Unveiling sequence-agnostic mixed-chemical modification patterns for splice-switching oligonucleotides using the NATURA platform by Tommaso Tabaglio, Taniya Agarwal, Wei Yuan Cher, Jin Rong Ow, Ah Keng Chew, Priscila Yun Qian Sun, Raja Sekhar Reddy Gurrampati, Hongfang Lu, Praveena Naidu, Hong Kai Ng, Xavier Le Guezennec, Shi Yan Ng, Manikandan Lakshmanan, Ernesto Guccione, Keng Boon Wee

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Nucleic Acid Aptamers for Human Norovirus GII.4 and GII.17 Virus-Like Particles (VLPs) Exhibit Specific Binding and Inhibit VLPs from Entering Cells by Cheng C, Sun M, Li J, Xue Y, Cai X, Liu J, Wang X, Xu S, Xie Y, Zhang J

“…Here, we screened nucleic acid aptamers against the widespread HuNoV GII.4 and emerging HuNoV GII.17.Methods: After ten rounds of sieving for HuNoV GII.4 and GII.17 virus-like particles (VLPs), eight ssDNA aptamers were generated and characterized for each genotype.Results: Four of the eight aptamers generated for GII.4 VLP had dissociation constants (Kd) less than 100 nM, and all aptamers for GII.17 VLP had Kd less than 10 nM. …”
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Design and application of a bar-code scanning device for nucleic acid testing (一种核酸检测用辅助扫码装置设计与应用) by ZHANG Hongling (张红伶), YU Xinying (虞新颖), JIANG Hongli (蒋宏利)

Subjects: “…nucleic acid testing…”
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Evaluation of One Step Nucleic Acid Amplification for detection of lymph node metastases compared to histopathological ultrastaging in women with endometrial cancer: a protocol for a diagnostic accuracy study by Jan Kostun, Alessandra Mescalchin, Martin Pešta, Robert Slunečko, Khaled M. Ismail, Vendula Smoligová, Jiri Presl

“…Abstract Background This is a multicentre, European, prospective trial evaluating the diagnostic accuracy of One Step Nucleic Acid Amplification (OSNA) compared to sentinel lymph nodes histopathological ultrastaging in endometrial cancer patients. …”
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Management modes and effects analysis of strengthening the volunteer’s normalized nucleic acid sampling (加强志愿者常态化核酸采样的管理方法与效果) by HAN Chunying (韩春英), YOU Chenggang (尤诚刚), PAN Meili (潘美丽), JIANG Yuting (蒋玉婷), XU Yiwen (徐伊雯), LU Weijie (陆玮婕), XIA Hui (夏卉), CHEN Haiyan (陈海燕), HUANG Ying (黄莹), GU Yuanlu (顾元露), CHEN Huiying (陈慧颖), ZHANG Qi (张琪), LI Xu (李旭), WU Weina (吴维娜), SHEN Xiaojie (沈晓洁), ZHOU Yi (周毅), ZHANG Xian (张宪), LI Qin (李琴)

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The amount of DNA and RNA in primary cancer cells and their metastases by Oleksandr Herasymenko, Kostyantyn Gerasymenko, Olha Herasymenko

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IMPLEMENTATION OF 454 LIFE SCIENCES TECHNOLOGY LABORATORY PRACTICES by N. G. Zinyakov, Ye. V. Ovchinnikova, S. P. Lazareva, A. A. Kozlov, I. A. Chvalas

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Development of a Copro-RPA-CRISPR/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method. by Guoqing Shao, Xiaowei Zhu, Ruiqi Hua, Yanxin Chen, Guangyou Yang

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An innovative mode of whole-process non-contact single-person sample collection of nucleic acid testing (一种全程无接触式单人核酸检测样本采集创新模式) by ZHANG Lijun (张丽君), TANG Ling (唐玲)

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Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota by Usman Hussain, Jim Downie, Amy Ellison, Sandra Denman, James McDonald, Marine C. Cambon

“…Abstract Background Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or binding within the amplified region of non-target DNA to stop the function of DNA polymerase. …”
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Assessing traditional Chinese medicine from a molecular perspective by Minhui Su, Wenxi Wang, Xudong Sun, Lihong Li, Weihong Tan

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A Century-Old Threat: Disseminated Tuberculosis in a Healthy Host – Case Report by Poongodi Santhana Kumarasamy, Bala Ponnuthurai, Stanley James Benjamin Swamidoss, Mohamed Arafath Mohamed

Subjects: “…cartridge-based nucleic acid amplification test…”
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A prospective study to evaluate the clinical specificity of the cobas® MPX test kit for screening for HIV RNA, HCV RNA, and HBV DNA in blood donation samples using the cobas® 6800... by Lei Zhou, Lin Wang, Xiaofang Gong, Xiaochun Liu, Yaxuan Zou, Yingying Wang, Jinfeng Zeng, Liang Zang

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Challenges in observing transcription–translation for bottom-up synthetic biology by Vadim G. Bogatyr, Gijs J. L. Wuite

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Hepatitis E virus infection among blood donors in Zhengzhou by ZHAO Hongna, WEI Yueguang, YAN Lumin, TU Tiantian, WANG Shumin, WEI Yihui, WANG Yifang, ZHAO Lei, CHEN Mingjun

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